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3,947 results for “Requirement”
Data from: Dynamic relocalization of replication origins by Fkh1 requires execution of DDK function and Cdc45 loading at origins
Chromosomal DNA elements are organized into spatial domains within the eukaryotic nucleus. For example, sites actively undergoing DNA replication, high-level transcription, and repair of double-strand breaks coalesce into foci, although the significance and mechanisms giving rise to these dynamic structures are poorly understood. In S. cerevisiae, replication origins exhibit characteristic subnuclear localizations prior to S phase that anticipate their initiation timing and/or efficiency during S phase: origins found within the nuclear interior in G1 phase initiate early and efficiently in S phase while origins found associated with the nuclear periphery in G1 phase initiate later and less efficiently. In this study, we link interior localization of replication origins in G1 phase with Fkh1 activity, which is required for their early replication timing. Using a Fkh1-dependent origin relocalization assay, we determine that execution of Dbf4-dependent kinase function, including Cdc45 loading, results in dynamic relocalization of a replication origin from the nuclear periphery to the interior in G1 phase. Origin mobility increases substantially with Fkh1-driven relocalization. These findings provide novel molecular insight into the mechanisms that govern dynamics and spatial organization of DNA replication origins and possibly other functional DNA elements.
APRICOT-1.0 files required for tutorial and demonstration
<p>This folder contains the basic files required to run the APRICOT pipeline for tutorial and demonstration purpose.</p> <p>APRICOT is a computational pipeline for the identification of specific functional classes of interest in large protein sets. The pipeline uses efficient sequence-based algorithms and predictive models like signature motifs of protein families for the characterization of user-provided query proteins with specific functional features. The dynamic framework of APRICOT allows the identification of unexplored functional classes of interest in the large protein sets or the entire proteome.</p> <p>The step-by-step tutorial that requires this dataset is available at: https://github.com/malvikasharan/APRICOT/blob/master/APRICOT_tutorial.md</p> <p>The source codes of APRICOT are available via git https://github.com/malvikasharan/APRICOT and pip https://pypi.python.org/pypi/bio-apricot.</p>
Optimization of global crop distribution could reduce greenhouse gas emissions by one third but requires expanding international trade
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REQUIREMENTS FOR PSYCHOLOGICAL AND PEDAGOGICAL TRAINING OF CADETS OF HIGHER MILITARY EDUCATIONAL INSTITUTIONS
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PEDAGOGICAL REQUIREMENTS OF TEACHING STUDENTS TO USE LANDSCAPE COMPOSITION IN PAINTING
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Removal of contaminants of emerging concern from the supernatant of anaerobically digested sludge by O3 and O3/H2O2: Ozone requirements, effects of the matrix, and toxicity
<p>Digestate is a rich source of nutrients that can be applied in agricultural fields as fertilizer or irrigation water.<br>However, most of the research about application of digestate have focused on its agronomic properties and<br>neglected the potential harm of the presence of contaminants of emerging concern (CECs). Aadvanced oxidation processes (AOPs) have proved to be effective for removing these compounds from drinking water, yet there are some constrains to treat wastewater and digestate mainly due to their complex matrix. In this study, the feasibility to remove different CECs from digestate using O3 and O3/H2O2 was assessed, and the general effect of the matrix in the oxidation was explained. While the lab-scale ozonation provided an ozone dose of 1.49 mg O3/mg DOC in 5 h treatment, almost all the compounds were removed at a lower ozone dose of maximum 0.48 mg O3/ mg DOC; only ibuprofen required a higher dose of 1.1 mg O3/mg DOC to be oxidized. The digestate matrix slowed down the kinetic ozonation rate to approximately 1% compared to the removal rate in demineralized water. The combined treatment (O3/H2O2) showed the additional contribution of H2O2 by decreasing the ozone demand by 59–75% for all the compounds. The acute toxicity of the digestate, measured by the inhibition of Vibrio fisheries luminescence, decreased by 18.1% during 5 h ozonation, and by 34% during 5 h O3/H2O2 treatment. Despite the high ozone consumption, the ozone dose (mg O3/mg DOC) required to remove all CECs from digestate supernatant was in the range or lower than what has been reported for other (waste-)water matrix, implying that ozonation can be considered as a post-AD treatment to produce cleaner stream for agricultural purposes.</p>
Data Supporting High-Level to Low-Level Requirements Coverage Reviewing with Large Language Models
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Combining Goal-Oriented Requirements Analysis and Testing for Software Energy-Awareness
<p>Artifacts for the paper "Combining GORE Analysis and Testing for Software Energy-Awareness"</p>
Data for: Evaluating Different i*-based Approaches for Selecting Functional Requirements while Balancing and Optimizing Non-Functional Requirements: A Controlled Experiment
<p>This dataset is associated with the study published in <em>Information and Software Technology</em>, Volume 106, February 2019, Pages 68-84. The study investigates the effectiveness of an alternative automated i* post-processed model for requirements engineering. The primary objective of the study was to provide empirical evidence that requirement engineers may perform better at selecting Functional Requirements (FRs) while optimizing and balancing Non-Functional Requirements (NFRs) using this post-processed model, compared to the original i* model.</p> <p>The data in this dataset was used for the analyses presented in the publication and includes the inputs required to evaluate the performance of requirement engineers in selecting FRs and optimizing NFRs. It provides the basis for comparing the decision-making efficiency and accuracy in FR selection, as well as the optimization process for NFRs in both the original and automated post-processed i* models.</p>
DRIP: Segmenting Individual Requirements from the Paragraphs in Software Requirement Documents
<p>Software Requirement Document datasets in past 5 years of RE, ICSE, REFSQ, REJ</p>
Distribution. Ivory Coast and Liberia. Its presence in SE Guinea requires confirmation. in Viverridae
Distribution. Ivory Coast and Liberia. Its presence in SE Guinea requires confirmation.
Geographic range estimates and environmental requirements for the harpy eagle derived from spatial models of current and past distribution
<p>Understanding species-environment relationships is key to defining the spatial structure of species distributions and develop effective conservation plans. However, for many species this baseline information does not exist. With reliable presence data, spatial models that predict geographical ranges and identify environmental processes regulating distribution are a cost-effective and rapid method to achieve this. Yet these spatial models are lacking for many rare and threatened species, particularly in tropical regions. The harpy eagle (<i>Harpia harpyja</i>) is a Neotropical forest raptor of conservation concern with a continental distribution across lowland tropical forests in Central and South America.Currently the harpy eagle faces threats from habitat loss and persecution and is categorised as Near-Threatened by the International Union for the Conservation of Nature (IUCN). Within a point process modelling (PPM) framework, we use presence-only occurrences with climatic and topographical predictors to estimate current and past distributions and define environmental requirements using Ecological Niche Factor Analysis. The current PPM prediction had high calibration accuracy (Continuous Boyce Index = 0.838) and was robust to null expectations (pROC ratio = 1.407). Three predictors contributed 96 % to the PPM prediction, with Climatic Moisture Index the most important (72.1 %), followed by minimum temperature of the warmest month (15.6 %) and Terrain Roughness Index (8.3 %). Assessing distribution in environmental space confirmed the same predictors explaining distribution, along with precipitation in the wettest month. Our reclassified binary model estimated a current range size 11 % smaller than the current IUCN range polygon. Paleoclimatic projections combined with the current model predicted stable climatic refugia in the central Amazon, Guyana, eastern Colombia, and Panama. We propose a data-driven geographical range to complement the current IUCN range estimate, and that despite its continental distribution this tropical forest raptor is highly specialized to specific environmental requirements.</p> <p> </p>
Inputs and outputs for monolayers simulations in "Accurate Simulations of Lipid Monolayers Require a Water Model With Correct Surface Tension"
<p>Inputs and outputs file for simulations of POPC and DPPC Monolayers at 298K. For details see:</p>
Supplementary material to "Food and habitats requirements of the Scops Owl (Otus scops) in Switzerland revealed by very high-resolution multi-scale models"
<p><strong>Abstract</strong></p> <p>In Europe, agricultural practices have progressively evolved towards high productivity leading either to the intensification of productive and accessible areas or to the abandonment of less profitable sites. Both processes have led to the degradation of semi-natural habitats like extensive grasslands, threatening species such as the Eurasian Scops Owl <em>Otus scops</em> that rely on extensively managed agricultural landscapes. In this work, we aimed to assess the habitat preferences of the Scops Owl using habitat suitability models combined with a multi-scale approach. We generated a set of multi-scale predictors, considering both biotic and abiotic variables, built on two newly developed vegetation management and orthopteran abundance models. To select the variables to incorporate in a ‘best multi-scale model’, we chose the best spatial scale for each variable using univariate models and by calculating their relative importance through multi-model inference. Next, we built ensembles of small models (ESMs) at 10 different scales from 50 to 1000 m, and an additional model with each variable at its best scale (‘best multi-scale model’). The latter performed better than most of the other ESMs and allowed the creation of a high-resolution habitat suitability map for the species. Scops Owls showed a preference for dry sites with extensive and well-structured habitats with 30–40% bush cover, and relied strongly on semi-extensive grasslands covering at least 30% of the surface within 300 m of the territory centre and with high orthopteran availability near the centre (50-m radius), revealing a need for good foraging grounds near the nest. At a larger spatial scale within a radius of 1000 m, the habitat suitability of Scops Owls was negatively related to forest cover. The resulting ESM predictions provide valuable tools for conservation planning, highlighting sites in need of particular conservation efforts together with offering estimates of the percentage of habitat types and necessary prey abundance that could be used as targets in future management plans to ensure the persistence of the population.</p>
Taxonomy of privacy requirements LGPD based
<p>A taxonomy of privacy requirements based on the LGPD, Brazilian privacy law.</p>
Supplementary material 3 from: Nelufule T, Robertson MP, Wilson JRU, Faulkner KT (2022) Native-alien populations—an apparent oxymoron that requires specific conservation attention. NeoBiota 74: 57-74. https://doi.org/10.3897/neobiota.74.81671
File S3
Supplementary material 2 from: Nelufule T, Robertson MP, Wilson JRU, Faulkner KT (2022) Native-alien populations—an apparent oxymoron that requires specific conservation attention. NeoBiota 74: 57-74. https://doi.org/10.3897/neobiota.74.81671
File S2
Supplementary material 1 from: Nelufule T, Robertson MP, Wilson JRU, Faulkner KT (2022) Native-alien populations—an apparent oxymoron that requires specific conservation attention. NeoBiota 74: 57-74. https://doi.org/10.3897/neobiota.74.81671
File S1
Distribution. NE Chile and NW Argentina; occurrence in SW Bolivia requires confirmation. in Cricetidae
Distribution. NE Chile and NW Argentina; occurrence in SW Bolivia requires confirmation.
Nicotine-mediated rescue of α-synuclein toxicity requires synaptic vesicle glycoprotein 2
<p>Tabular data underlying all figures. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.