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111,247 results for “cells”
Fig. 10 in Effects of Roscovitine on Schedule of Divisional Morphogenesis, Basal Bodies Proliferation and Cell Divisions in Tetrahymena thermophila
Fig. 10. Western blot of T. thermophila fractions with monoclonal antibody anti-cdc14A. H – homogenate, S – supernatant, C – cortical fraction (pellet).
Fig. 2 in Sarcocystis falcatula-like derived from opossum in Northeastern Brazil: In vitro propagation in avian cells, molecular characterization and bioassay in birds
Fig. 2. (A) A mature schyzont of Sarcocystis falcatula-like (Sarco-BA1 strain) in a permanent chicken cell line (UMNSAH/DF-1). May-Grüenwald-Giemsa stain. Bar = 20 μm. (B) Extracellular merozoites of Sarco-BA1 on a monolayer of UMNSAH/DF-1 cells. Bar = 10 μm.
Fig. 1 in Sarcocystis falcatula-like derived from opossum in Northeastern Brazil: In vitro propagation in avian cells, molecular characterization and bioassay in birds
Fig. 1. Sporocyst of Sarcocystis falcatula-like. Four sporozoites are visualized inside the sporocyst by light microscopy (A). Autofluorescence of the sporocyst wall is observed after excitation with ultraviolet on a fluorescence microscope (B).
Text-fig. 2: Microscopic photo of the wood from Kučlín (specimen No. G 4723, NM, transverse section) shoving growth ring boundary with markedly rounded tracheids and abundant axial parenchyma (dark cells) present both in late- and earlywood (scale bar = 100 µm). in Silicified Stem From The Late Eocene Fossil Locality Of Kučlín (Czech Republic): Overview And New Remarks
Text-fig. 2: Microscopic photo of the wood from Kučlín (specimen No. G 4723, NM, transverse section) shoving growth ring boundary with markedly rounded tracheids and abundant axial parenchyma (dark cells) present both in late- and earlywood (scale bar = 100 µm).
Figure 1 in High Efficiency All-Polymer Solar Cells
Figure 1. – Location (✱) of the catch of Desmodema polystictum in the Northwestern Indian Ocean waters.
Altered T-cell Reactivity to β-amyloid-related Antigens in Early Alzheimer's Disease
<p>The code generated during this study is available at this online repository with free access. Further information and requests for resources should be directed to and will be fulfilled by the Lead Contact, Dr. Christoph Gericke (christoph.gericke@irem.uzh.ch).</p>
HIV-1 control in vivo is related to the number but not the fraction of infected cells with viral unspliced RNA
<p>In the absence of antiretroviral therapy (ART), a subset of individuals, termed HIV controllers, have levels of plasma viremia that are orders of magnitude lower than non-controllers who are at higher risk for HIV disease progression. In addition to having fewer infected cells resulting in fewer cells with HIV RNA, it is possible that lower levels of plasma viremia in controllers is due to a lower fraction of the infected cells having HIV-1 unspliced RNA (HIV usRNA) compared with non-controllers. To directly test this possibility, we used sensitive and quantitative single cell sequencing methods to compare the fraction of infected cells that contain one or more copies of HIV usRNA in peripheral blood mononuclear cells (PBMC) obtained from controllers and non-controllers. The fraction of infected cells containing HIV usRNA did not differ between the two groups. Rather, the levels of viremia were strongly associated with the total number of infected cells that had HIV usRNA, as reported by others, with controllers having 34-fold fewer infected cells per million PBMC. These results reveal for the first time that viremic control is not associated with a lower fraction of proviruses expressing HIV usRNA, unlike what is reported for elite controllers, but is only related to having fewer infected cells overall, maybe reflecting greater immune clearance of infected cells. Our findings show that proviral silencing is not a key mechanism for viremic control and will help to refine strategies towards achieving HIV remission without ART.</p>
Data sets for de Oliveira et al.: "Leishmania major telomerase RNA knockout: from altered cell proliferation to decreased parasite infectivity"
<p><strong><span>This file contains relevant data about the article: "</span></strong><em><span>Leishmania major</span></em><strong><span> telomerase RNA knockout: from altered cell proliferation to decreased parasite infectivity"</span></strong></p>
Dataset of single-cell transcriptomic matrix of 10 human glioblastoma tissue
<p>Dataset of single-cell transcriptomic matrix of 10 human glioblastoma tissue. <span>scRNA-seq was performed using the droplet-based 10x Genomics platform</span><span> </span><span>(10x Genomics, Pleasanton, CA, USA)<span>. <span>GBM tissues for single-cell RNA sequencing (<a name="_Hlk147870629"></a>scRNA-seq)</span> were collected from patients admitted to Xiangya Hospital, Central South University.</span></span></p>
3D super-resolution datasets associated with the paper "Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet"
<p>3D single-molecule super-resolution datasets corresponding to reconstructions shown in <em>Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet</em> by Saliba & Gagliano, Gustavsson et. al.</p>
Single-cell and spatial transcriptomics delineate molecular traits and immunosuppressive landscape during histological progression of lung adenocarcinoma
<p>Two specimens of lung adenocarcinoma, each corresponding to the lepidic and solid histologic patterns as confirmed through histologic scrutiny, were procured in accordance with standard surgical protocols. These specimens underwent a process of formalin fixation and were subsequently encapsulated within paraffin-embedded tissue blocks. The specimens were then sectioned and subjected to hematoxylin and eosin (H&E) staining to facilitate subsequent imaging at a resolution of 40x (equivalent to 0.25 micron/pixel) via the use of Aperio GT450 scanners. The tissue slides were then conveyed to the Genomics core, where following the decoverslipping of the tissue, the Visium CytAssist device was employed to transfer transcriptomic probes from the original glass slides to capture areas on Visium slides measuring 11mm x 11mm. Comprehensive transcriptomic profiling was achieved post mRNA permeabilization, through poly(A) capture and probe hybridization. The resultant libraries were sequenced utilizing the Illumina Novaseq 6000, using paired-end sequencing with a read length of 150 base pairs.</p>
Figure 1 in Blood cells and some hematological parameters of red drum (Linnaeus, 1766) in Vietnam
Figure 1. Erythrocytes of red drum. a - location L1, b - location L2, c - location L3. The red arrow indicates the deformed erythrocyte nuclear/uneven nuclear-matter distribution. The blue arrow indicates the slightly alkaline erythrocytes.
Genome Sizes of Bacterial Species Detected in Cell-Free DNA of Patients with Acute Leukemia and Sepsis, Including Those Undergoing Bone Marrow Transplantation
<p>Next Generation Sequencing (NGS) analysis of Cell-Free DNA provides valuable insights into a spectrum of pathogenic species (particularly bacterial) in blood. Patients with Sepsis often face problems like delays in treatment regimens (combination or cocktail of antibiotics) due to the long turnaround time (TAT) of classical and standard blood culture procedures. NGS gives results with lower TAT along with high-depth coverage. The use of NGS may be a possible solution to deciding treatment regimens for patients without losing precious time and more accurately possibly saving lives.</p> <p>Our curated dataset is of bacterial species or strains detected along with their genome size in 107 AML patients diagnosed with Sepsis clinically. Cell-free DNA profiles of patients were built and sequencing was done in Illumina (NovaSeq and NextSeq). Bioinformatic analysis was performed using two classification algorithms namely kraken2 and kaiju. For kraken2 based classification reference bacterial index developed by Carlo Ferravante et al (Zenodo 2020) (link: https://zenodo.org/records/4055180) was used, while for kaiju-based classification reference database named "nr_euk" dated "2023-05-10" (link: https://bioinformatics-centre.github.io/kaiju/downloads.html) was used.</p> <p>Genome size annotation is important in metagenomics since for the use of depth of coverage (abundance), genome size is required. In metagenomic classification algorithms like kraken/kraken2 and kaiju output computes reads assigned only and not abundance. In kaiju, the problem is more complicated since the reference database does not have a fasta file but only an index file from which alignment is done. </p> <p>To address the above challenges to compute "depth of coverage" or simply abundance, we build a Genome size annotator tool (https://github.com/patkarlab/Genome-Size-Annotation) which provides genome size for each species detected given its taxid is available. In this tool, the NCBI Datasets tool, NCBI Genome API check tool, and Data Mining from AI search engines like perplexity.ai are used. </p> <p>We have curated two datasets</p> <p>Kraken2 dataset named "FINAL METAGENOMIC DATA MASTERSHEET - kraken_genome_annotation"<br>Kaiju dataset named "FINAL METAGENOMIC DATA MASTERSHEET - kaiju_genome_annotation"</p> <p>*Please note that for kraken2 curated dataset, we used data mining from the AI search engine perplexity.ai while for kaiju we did not use perplexity, ai, and any species whose genome size was not found was labeled "NA"</p>
scPerturb Single-Cell Perturbation Data: RNA and protein h5ad files
<p>Collection of h5ad files for RNA and protein single-cell perturbation datasets on scPerturb.org.</p> <p>For the associated publication, see <a href="https://www.nature.com/articles/s41592-023-02144-y">https://www.nature.com/articles/s41592-023-02144-y</a>.</p> <p>H5ad files were created using scanpy 1.9.1, using gzip compression.</p> <p> </p>
FVLLMONTI Application examples: Single reconfigurable VNWFET TCAD simulation and Reference logic cell DTCO flow
<p>This data set presents two application examples collecting and highlighting some of the key components to the TCAD, compact modelling and DTCO approaches carried out as part of the FVLLMONTI project in relation to Deliverable D3.2.</p> <p>First we present an example that demonstrates the functionality of the U-shaped reconfigurable transistor, switching the device behaviour between NMOS and PMOS using the Program Gate and giving the transistor characteristics of those devices.</p> <p>Secondly, this data set gives an introduction into the FVLLMONTI DTCO approach. The provided simulation project shows a reference DTCO flow from layout to library characteristics for previously delivered physical logic cell designs. The DTCO flow provides insight into logic cell parasitics, netlist for 3rd party SPICE analysis, and KPI determination and characterization.</p> <p>Both simulation projects can be seen as template for similar setups employed to overcome the challenges and answer the detailed questions faced in investigating a newly developed semiconductor technology such as the FVLLMONTI stack.</p>
Data for device simulation in the article "Analysing the impact of the hole transport layer on the space charge distribution and hysteresis in perovskite solar cells using capacitance-voltage profiling"
<p>This repository contains the data used to perform device simulation in the article "Analysing the impact of the hole transport layer on the space charge distribution and hysteresis in perovskite solar cells using capacitance-voltage profiling", submitted in September 2024 to the journal Sustainable Energy and Fuels.</p> <p><br>The authors of this data and the article are E. Regalado-Pérez, Evelyn B. Díaz-Cruz, and J. Villanueva-Cab </p> <p><br>The scripts (.m) and input files (.csv) hosted here are based on the files created by the authors of the Driftfusion code, which can be found in the GitHub repository "barnesgroupICL/Driftfusion" at https://github.com/barnesgroupICL/Driftfusion.</p> <p> </p>
Lithium-Ion Battery Field Data: 28 LFP battery systems with 8 cells in series, up to 5 years of operation
<div> <div> <div>This data set contains data from 28 portable 24V lithium iron phosphate (LFP) battery systems with approximately 160Ah nominal capacity. Each system's specific use case is unknown, but battery systems of this size are typically used as power sources for recreational vehicles, solar energy storage, and more.</div> <br> <div>All battery systems in this data set showed some form of unsatisfactory behavior and were returned to the manufacturer. Many reasons can cause a consumer to return a battery to the manufacturer for maintenance. The user's individual decisions may be motivated by personal judgment, BMS warnings, or customer support advice. This data set comprises a very small fraction of batteries sold of this version. Therefore, this data set is biased and not representative of the operational data of the entire population of this system version. An improved version replaced this battery system type. The battery system manufacturer provided the data set for this study and allowed its open-source release under the condition of anonymity.</div> <br> <div>Each battery system consists of 8 prismatic cells in series. Each system has one load current sensor, and each cell has one voltage sensor. The four temperature sensors are placed between adjacent cells, i.e., each temperature sensor is shared by two cells. Furthermore, the battery systems have active cell balancing. The available measurements vary from a single month to five years. Consequently, the number of data rows per system varies from several thousand to millions, depending on the duration of battery operation. The data set contains a total of 133 million rows of measurements.</div> </div> </div> <div> </div> <div> <div><strong>Associated Python Library</strong></div> <div>BattGP <a href="https://github.com/JoachimSchaeffer/BattGP" target="_blank" rel="noopener">https://github.com/JoachimSchaeffer/BattGP</a></div> <div>This library contains classes and functions to analyze the data set with Gaussian processes.</div> <div>Furthermore, data visualization functions are part of the library.</div> <div><br><strong>Associated Article<br></strong>Gaussian Process-based Online Health Monitoring and Fault Analysis of Lithium-Ion Battery Systems from Field Data</div> <div>Cell Report Physical Science <br> <div><a href="https://doi.org/10.1016/j.xcrp.2024.102258">https://doi.org/10.1016/j.xcrp.2024.102258</a></div> <div> </div> <div> </div> </div> </div>
Thin blood smear images of red blood cells with rouleaux formation morphology and normal morphology
<p>This dataset contains images of thin blood smear with normal red blood cell morphology and rouleaux red blood cell morphology. Ethical approval with approval number: NHREC/17/03//2018 was obtained from Kano state ministry of health. Blood samples from 100 malaria infected patients were collected from Asiya Bayero pediatric hospital, kano state, Nigeria. Thick and thin blood smear slides were prepared using field stain. To ensure there was no bias in slide preparation, slides used for hospital diagnosis prepared under limited and constrained conditions were used as such types of slides represent the true reality of malaria diagnosis in less developed countries.Thin blood smear microscopy was performed by an expert microscopist and each slide was labeled according to the presence of Rouleaux formation or not among others. Out of 100 samples collected, 28 samples had rouleaux formation morphology.</p> <p>A 12MP iPhone 10 camera was attached to a microscope’s eyepiece. Pictures of different field of views for each slide were captured using the iPhone’s camera. For each slide, a minimum of 10 different field of views were captured. 616 images were captured for slides with rouleaux formation. To create a balanced dataset an equal number, 616 images were also captured for slides with normal morphology. To increase the size and variation of the dataset. 312 Digital images of thin blood smear slides with Giemsa staining collected from Murtala Muhammad specialist hospital were added. out of the 312 images, 156 had rouleaux RBC morphology and 156 had normal RBC morphology. Image capture was conducted in the morning, afternoon and evening and in different rooms with different lighting conditions to introduce diverse levels of illumination in the images The captured images from both hospitals had a size of 4032x3024 pixels. The background of the images were cropped to give a size 2500x2500 which were then sliced to give a final size of 750x750 pixels. The final data set consists of 12,356 thin blood smear images with rouleaux formation morphology and 12,356 thin blood smear images with normal red blood cell morphology. Different CNN architectures were trained for the binary classification of the dataset.</p>
Discrete Flow Cytometry of Depth Profile Samples from TN413 (2023) Using a BD Influx Cell Sorter
<p>The dataset consists of BD Influx-based analysis of phytoplankton populations from discrete flow cytometry data collected underway during the University of Washington School of Oceanography 2023 undergraduate senior thesis cruise (TN413) oceanographic research cruise from Hawaii to Fiji. The data consists of cell abundance, cell size (equivalent spherical diameter), carbon quota, and carbon biomass for heterotrophic bacteria, picophytoplankton populations, namely the cyanobacteria Prochlorococcus and Synechococcus, and small eukaryotic phytoplankton (<5 μm ESD). Time is in UTC format, latitude and longitude are in decimal degrees, and depth is in meters. Further information can be found here: https://github.com/fribalet/FCSplankton</p>
Complex excitability and ``flipping" of granule cells: an experimental and computational study
<p>Electrophysiology dataset for Complex excitability and ``flipping" of granule cells: an experimental and computational study.</p> <p>In response to prolonged depolarizing current steps, different classes of neurons display specific firing characteristics (i.e., excitability class), such as a regular train of action potentials with more or less adaptation, delayed responses, or bursting. In general, one or more specific ionic transmembrane currents underlie the different firing patterns. Here, we sought to investigate the influence of artificial sodium-like (Na channels) and slow potassium-like (KM channels) voltage-gated channels conductances on firing patterns and transition to depolarization block (DB) in Dentate Gyrus granule cells with dynamic clamp - a computer-controlled real-time closed-loop electrophysiological technique, which allows to couple mathematical models simulated in a computer with biological cells. Our findings indicate that the mimicked extra Na/KM channels significantly affect the firing rate of low frequency cells, but not in high-frequency cells. Moreover, we have observed that 44 percent of recorded cells exhibited what we have called a ``flipping'' behavior. This means that these cells were able to overcome the DB and generate trains of action potentials at higher current injection steps. We have developed a mathematical model of ``flipping" cells to explain this phenomenon. Based on our computational model, we conclude that the appearance of ``flipping" is linked to the number of states for the sodium channel of the model.</p>
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.