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Supplementary material 2 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Tables S2, S3

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Figure 9 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 9 Hogna heeri female (CRBALC0501): A epigyne, ventral B vulva, dorsal. Abbreviations, female genitalia: D – diverticulum, H – epigynal hoods, MS – median septum, S – spermatheca. Scale bars: 0.2 mm.

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Figure 8 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 8 Distribution of H. blackwalli. Circles: present records; black triangles: revised records from literature; white triangles: unconfirmed records from literature.

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Figure 7 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 7 Photograph of H. blackwalli. Female specimen, recently dead, in captivity. Photograph credit Emídio Machado.

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Figure 3 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 3 Ultrametric tree for the COI obtained with BEAST using a coalescent (constant population growth) prior to apply the GMYC model. Only unique sequences included. Support on nodes should be read as follows: black: BI posterior probability ≥ 0.95; grey: BI posterior probability < 0.95. Species delimitations based on alternative approaches are indicated with boxes besides the terminal labels.

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Figure 28 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 28 Unidentified male specimens belonging to the H. maderiana / H. insularum complex from Porto Santo. Left male pedipalps, ventral A CRBALC0328 B CRBALC0345. Scale bar: 0.5 mm (A).

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Figure 29 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 29 Unidentified female specimens belonging to the H. maderiana / H. insularum complex from Porto Santo. Female genitalia A, B CRBALC0329 A epigyne, ventral B vulva, dorsal C, D CRBALC0346 C epigyne, ventral D vulva, dorsal. Scale bar: 0.5 mm (A).

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Figure 6 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 6 Hogna blackwalliA–C male (CRBALC0718): A left male pedipalp, ventral B detail of the median apophysis, anteroventral CSEM image, right male pedipalp, ventral D, E female (CRBALC0516): D epigyne, ventral (white arrow points to an indentation that may be helpful for diagnosis) E vulva, dorsal. Abbreviations, male pedipalp: AT – anterior point, C – cymbium, E – embolus, P – palea, R – ridge, T – tegulum, TA – terminal apophysis, TgA – tegular apophysis, VS – ventral spur. Abbreviations, female genitalia: D – diverticulum, H – epigynal hoods, MS – median septum, S – spermatheca. Scale bars: 0.5 mm (A, D, E); 0.2 mm (B, C).

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Figure 27 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 27 Plate with photographs of the lateral view of the leg I for the complex of H. maderiana, H. insularum and their intermediate forms.

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Figure 25 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 25 Distribution of H. nonannulata. Black circle: present record; white triangle: unconfirmed record from literature.

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Figure 22 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 22 Photograph of H. maderiana. Female specimen in the field. Photograph credit Pedro Cardoso.

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Figure 21 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 21 Hogna maderianaA–D male (CRBALC0734): A left male palp, ventral (white arrow points to a tegular concavity that may be helpful for diagnosis) B detail of the median apophysis, anteroventral CSEM image, right male palp, ventral D idem, retroventral E, F female (CRBALC0717): E epigynum, ventral F vulva, dorsal. Abbreviations, male pedipalp: AT – apical point, C – cymbium, E – embolus, P – palea, R – ridge, T – tegulum, TA – terminal apophysis, TgA – tegular apophysis, VS – ventral spur. Abbreviations, female genitalia: D – diverticulum, H – epigynal hoods, MS – median septum, S – spermatheca. Scale bars: 0.5 mm (A, B, E, F); 0.2 mm (C, D).

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Figure 4 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 4 COI haplotype (upper) and ITS-2 allele (lower) networks inferred under statistical parsimony (0.95 probability). Pie size proportional to number of individuals which exhibited the same haplotype/alleles. White circles represent missing haplotypes/alleles. Colours correspond to islands (colour codes in upper box). For the COI haplotypes only the network (3) including H. insularum / H. maderiana haplotypes showed (each remaining nominal species were resolved as independent networks). ITS-2 alleles boxed per species, except for H. insularum / H. maderiana. Haplotype/allele labels for H. insularum in bold and italics, H. maderiana in condensed bold and italics, not assigned in light italics (see lower box legend).

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Figure 24 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 24 Hogna nonannulata male (CRBALC0701): A left male pedipalp, ventral B detail of the median apophysis, anteroventral CSEM image, right male pedipalp, ventral. Abbreviations, male pedipalp: AT – apical point, C – cymbium, E – embolus, P – palea, R – ridge, T – tegulum, TA – terminal apophysis, TgA – tegular apophysis, VS – ventral spur. Scale bars: 0.5 mm (A); 0.2 mm (B, C).

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Figure 20 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 20 Distribution of H. isambertoi sp. nov. Circles: present records; black triangle: revised record from literature.

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Supplementary material 1 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Table S1. Primers used for amplification

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Figure 23 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 23 Distribution of H. maderiana. Black circles: present records; dotted circles: records from only leg samples; black triangles: revised records from literature; white triangles: unconfirmed records from literature.

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Figure 5 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 5 Species tree for the Madeiran Hogna including two outgroups. Values on nodes are estimated divergence times in millions of years (my). Dots on nodes indicate BI posterior probability >0.95. Bars correspond to the 95%HPD of the time estimates.

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Figure 2 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 2 Best maximum likelihood tree of Lycosinae, inferred with IQTREE2 after selecting the best partition scheme and evolutionary models. Nodes are split in three sections, representing the different methods. Support on nodes should be read as follows: black: ML ultrafast bootstrap and BI posterior probability ≥ 0.95, MP Jackknife ≥ 0.7; grey: ML Ultrafast Bootstrap and BI posterior probability < 0.95, MP Jackknife < 0.7; white: unrecovered node.

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Figure 19 from: Crespo LC, Silva I, Enguídanos A, Cardoso P, Arnedo M (2022) Island hoppers: Integrative taxonomic revision of Hogna wolf spiders (Araneae, Lycosidae) endemic to the Madeira islands with description of a new species. ZooKeys 1086: 84-135. https://doi.org/10.3897/zookeys.1086.68015

Figure 19 Hogna isambertoi sp. nov. A male habitus, dorsal (SMF) B female habitus, dorsal (SMF). Scale bars: 1 mm.

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