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Figure 5 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 5 Peinaleopolynoe santacatalina specimens ASIO-BIC A8487, live dorsal view BPeinaleopolynoe santacatalina observed on the Rosebud Whalefall off the coast of San Diego, CA CSIO-BIC A8489, live dorsal view without elytra. Numbers next to the elytrophores indicate the pairs of elytra (ten total) DSIO-BIC A10927, live ventral view. Arrows indicate the four pairs of papillae on segments 12–15. Abbreviations: el, elytrophore; br, single large group of branchiae on elytrigerous segment; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment. Scale bars: 5 mm (A, C, D); 10 mm (B).

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Figure 16 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 16 Micrographs of P. mineoi sp. nov. holotype SIO-BIC A10071 and paratype SIO-BIC A9709 A dorsal view, holotype B ventral view, holotype. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae and jaws, paratype. Numbers mark the papillae on the dorsal (seven papillae) and ventral (six papillae) surfaces D loose elytron, holotype E dorsal view of anterior, holotype F ventral view of anterior, holotype G right side branchiae on segments 8–13, holotype H ventral papillae on segments 12–15 (four pairs) indicated by arrows, holotype I dorsal view of posterior, holotype J ventral view of posterior, holotype. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; j, jaws; ma, median antenna; la, lateral antenna; pa, palp; dac, dorsal anterior cirrus; vac, ventral anterior cirrus; el, elytrophore; br, branchiae; nec, neurochaetae; noc, notochaetae; dc, dorsal cirrus; no, notopodium; vc, ventral cirrus; bc, buccal cirrus; ne, neuropodium; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 1 mm (A, B, D); 0.5 mm (C, E–J).

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Figure 4 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 4 Maximum likelihood tree topology from concatenated data (COI, 16S, 18S, 28S, H3, CytB) for Peinaleopolynoe and its sister clade (B. bipapillata and B. sp. nov. 1), with the transformation for A ventral segmental papillae and/or lamellae and B elytral pairs. 'Pie charts' at the nodes represent probabilities for the relevant states.

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Figure 9 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 9 Micrographs of P. orphanae sp. nov. holotype SIO-BIC A6151 A left parapodium from segment 6 B left parapodium from segment 9 C notochaeta D superior neurochaeta (supra-acicular) E inferior neurochaeta (subacicular). Abbreviations: br1, branchiae small group 1 attached to dorsal tubercle; br2, branchiae large group 2 attached near base of notopodium; noc, notochaetae; dc, dorsal cirrus; no, notopodium; snec, superior neurochaetae; ne, neuropodium; neap, neuroacicular process; vc, ventral cirrus; inec, inferior neurochaetae; el, elytrophore; br, single large group of branchiae on elytrigerous segment. Scale bars: 2 mm (A, B); 15 μm (C); 10 μm (D, E).

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Figure 13 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 13 Micrographs of P. elvisi sp. nov. holotype SIO-BIC A8488 A right parapodium from segment 10 B right parapodium from segment 7 C notochaetae D superior neurochaetae (supra-acicular) E inferior neurochaetae (subacicular). Abbreviations: br1, branchiae small group 1 attached to dorsal tubercle; br2, branchiae large group 2 attached near base of notopodium; noc, notochaetae; dc, dorsal cirrus; no, notopodium; snec, superior neurochaetae; ne, neuropodium; neap, neuroacicular process; noap, notoacicular process; vc, ventral cirrus; inec, inferior neurochaetae; el, elytrophore; br, single large group of branchiae on elytrigerous segment. Scale bars: 1 mm (A, B); 15 μm (C); 10 μm (D, E).

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Figure 3 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 3 Haplotype networks from COI data with geographic locality coding; each colored circle represents a single individual APeinaleopolynoe santacatalina network includes four individuals, three from the Rosebud Whalefall off San Diego, California and one from the Del Mar Seeps, California BPeinaleopolynoe mineoi sp. nov. network includes four individuals, three from Mound 12, Costa Rica and one from Mound 11, Costa Rica CPeinaleopolynoe elvisi sp. nov. includes five individuals, two from Jaco Scar, Costa Rica, two from Seamount 1, Costa Rica, and one from the Patrick Whalefall in Monterey Canyon, California. The small black circle represents a missing haplotype.

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Figure 12 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 12 Macro photos and micrographs of P. elvisi sp. nov. holotype SIO-BIC A8488 A dorsal view B ventral view. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae and jaws. Numbers mark the papillae on the dorsal (six papillae) surface D left elytron from segment 2 E dorsal view of anterior F ventral view of anterior G left side branchiae on segments 7–11 H ventral papillae on segments 12–15 (four pairs) indicated by white arrows. Ventral lamellae on segments 16–17 (two pairs) indicated by black arrows I dorsal view of posterior J ventral view of posterior. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; j, jaws; ma, median antenna; pa, palp; la, lateral antenna; vac, ventral anterior cirrus; el, elytrophore; br, single large group of branchiae on elytrigerous segment; noc, notochaetae; dc, dorsal cirrus; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment; no, notopodium; nec, neurochaetae; vc, ventral cirrus; ne, neuropodium; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 4 mm (A, B); 0.5 mm (C); 1 mm (D, H–J); 2 mm (E–G).

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Figure 8 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 8 Macro photos and micrographs of P. orphanae sp. nov. holotype SIO-BIC A6151 and paratype SIO-BIC A9996 A dorsal view, holotype B ventral view, holotype. Segments 12–15 are marked to indicate the presence of four pairs of papillae C frontal view of proboscis showing papillae, paratype. Numbers mark the papillae on the dorsal (seven papillae) and ventral (six papillae) surfaces D right elytron from segment 5, holotype E dorsal view of anterior, holotype F ventral view of anterior, holotype G right side branchiae on segments 8–11, holotype H ventral papillae on segments 12–15 (four pairs) indicated by arrows, holotype I dorsal view of posterior, holotype J ventral view of posterior, holotype. Abbreviations: XII, segment 12; XIII, segment 13; XIV, segment 14; XV, segment 15; ma, median antenna; la, lateral antenna; pa, palp; dac, dorsal anterior cirrus; vac, ventral anterior cirrus; el, elytrophore; br, single large group of branchiae on elytrigerous segment; noc, notochaetae; dc, dorsal cirrus; vc, ventral cirrus; bc, buccal cirrus; br1, branchiae small group 1 attached to dorsal tubercle on cirrigerous segment; br2, branchiae large group 2 attached near base of notopodium on cirrigerous segment; no, notopodium; ne, neuropodium; nec, neurochaetae; dt, dorsal tubercle; anc, anal cirrus. Scale bars: 4 mm (A, B); 2 mm (C–J).

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Figure 7 from: Hatch AS, Liew H, Hourdez S, Rouse GW (2020) Hungry scale worms: Phylogenetics of Peinaleopolynoe (Polynoidae, Annelida), with four new species. ZooKeys 932: 27-74. https://doi.org/10.3897/zookeys.932.48532

Figure 7 Live dorsal views of the new Peinaleopolynoe spp. APeinaleopolynoe orphanae sp. nov. holotype SIO-BIC A6151 BPeinaleopolynoe elvisi sp. nov. holotype SIO-BIC A8488 CPeinaleopolynoe goffrediae sp. nov. holotype SIO-BIC A5485 DPeinaleopolynoe mineoi sp. nov. holotype SIO-BIC A10071. Scale bars: 6 mm (A); 8 mm (B, C); 1 mm (D).

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Supplementary material 2 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Fasta 2

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Figure 6 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 6 Immature stages of Gordius chiashanus sp. nov. A, B free-living larva (A) treated with hot water and a living larva showing the depression in the anterior end of the pseudointestine (arrow) C, D eggs with the inner membrane examined using an (C) SEM and (D) compound microscope E egg strings F–H cysts in the paratenic host with (F) a unfolded larva and (G) a folded larva, showing (H) a single posterior spine (arrow) after treatment with a 5% KOH solution. Abbreviations: Ho, hooklet; PostS, postseptum; PreS, preseptum; Pro, proboscis; PsI, pseudointestine. Scale bars: 50 µm (A–D, F–H), 1 mm (E).

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Figure 7 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 7 Phylogenetic relationship of Gordius/Acutogordius spp. restructured using COI partial sequences compared with C. formosanus, E. nigromaculatus, and P. diblastus as out groups. Numbers at the nodes represent the percentage of 1000 bootstrap replicates.

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Supplementary material 1 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Video S1

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Figure 5 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 5 Field observation of Gordius chiashanus sp. nov. A hazy appearance (arrows) surrounding the body surface in hot water B spermatophore (arrow) on a female collected on the surface of the soil C rainbow-like reflection on the body surface D free-living adult collected in wet soil E, F infected host, Spirobolus sp. nov. (Hsu and Chang, unpublished), harboring (E) three immature worms (arrow) and (F) an adult worm. Photographs courtesy of (D) Fang, Hua-Te and (F) Hung, Ming-Chin. Scale bars: 1 cm (E).

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Figure 8 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 8 Seasonal occurrence of free-living adults of Gordius chiashanus sp. nov. Numbers at the bottom indicate the actual number of each bar.

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Figure 4 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 4 Female Gordius chiashanus sp. nov. A, B anterior end examined using a (A) stereomicroscope and (B) SEMC–E posterior end with the terminal view examined using a (C) stereomicroscope and (D) SEM, and the (E) lateral view examined using a stereomicroscope F, G mid-body examined using a (F) stereomicroscope and (G) compound microscope. Co, cloacal opening. Scale bars: 1 mm (A, F, G), 200 μm (B–D).

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Figure 2 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 2 Posterior end of male Gordius chiashanus sp. nov. A stereomicroscopic image of the posterior end B–DSEM images of (B) overview of the posterior end with bristles concentrated on the (C) lobe tips (arrow), and (D) inner side of the lobe tips and the formation of a bristle field on each tail lobe posterior to the tips of the postcloacal crescent (arrows) E cloacal opening with areoles on the inside wall. Scale bars: 1 mm (A), 500 μm (B), 200 μm (C–D), 50 μm (E).

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Figure 3 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 3 Mid-body of male Gordius chiashanus sp. nov. A, BSEM images of (A) cuticle in the mid-body with scattered short bristles (arrows) and (B) close-up view of a short bristle C, D white spots and dorsal and ventral dark pigmented line examined using (C) a compound microscope and (D) a stereomicroscope. Scale bars: 1 mm (A, C, D), 5 μm (B).

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Figure 1 from: Chiu M-C, Huang C-G, Wu W-J, Lin Z-H, Chen H-W, Shiao S-F (2020) A new millipede-parasitizing horsehair worm, Gordius chiashanus sp. nov., at medium altitudes in Taiwan (Nematomorpha, Gordiida). ZooKeys 941: 25-48. https://doi.org/10.3897/zookeys.941.49100

Figure 1 Anterior end of male Gordius chiashanus sp. nov. A stereomicroscopic image of the ventral side of the anterior end showing a white cap, dark-brown collar, and vertical white stripe on the ventral side B, CSEM images of the anterior end surface that is (B) smooth with scattered short bristles and (C) wrinkled D close-up view of the dotted square in C showing the short bristles (arrows) covered by a wrinkled structure. Scale bars: 2 mm (A), 200 μm (B–D).

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Proteo-transcriptomic analysis identifies potential novel toxins secreted by the predatory, prey-piercing ribbon worm Amphiporus lactifloreus

<p>Nemerteans (ribbon worms) employ toxins to subdue their prey, but research thus far has focused on the small-molecule components of mucus secretions and few protein toxins have been characterized. We carried out a preliminary proteotranscriptomic analysis of putative toxins produced by the hoplonemertean <em>Amphiporus lactifloreus </em>(Hoplonemertea, Amphiporidae). We did not find any variants of known nemertean-specific toxin proteins (neurotoxins, cytotoxins, parbolysins or nemertides) but we identified several toxin-like transcripts expressed strongly in the proboscis, including putative metalloproteinases and sequences resembling sea anemone actitoxins, crown-of-thorn sea star plancitoxins, and multiple classes of inhibitor cystine knot/knottin family proteins. Some of these products were also directly identified in the mucus proteome, supporting their preliminary identification as secreted toxin components. We identified two new nemertean-typical toxin candidates and named them U-nemertotoxin-1 and U-nemertotoxin-2. Our findings provide insight into the largely overlooked venom system of nemerteans and support a hypothesis in which the nemertean proboscis evolved in several steps, from a flesh-melting organ in scavenging nemerteans to a flesh-melting and toxin-secreting venom apparatus in hunting hoplonemerteans.</p>

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Last verified 2026-04-30Open record

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Last verified 2026-04-30Open record

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dandi-nwb
electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.
Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

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Last verified 2026-04-29Open record

OpenNeuro

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openneuro
neuroscienceopenPublished datasets are available on demand over the internet.
Last verified 2026-04-29Open record

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