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Figure 12 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 12 Scanning electron micrographs of the forebody of Horologion hubbardiA head of voucher CWH-452, dorsal aspect, clypeal setae indicated by white arrows B prosternum of non-type specimen, posterior aspect (head, pronotum and most of leg segments removed) C forebody of voucher CWH-452, ventral aspect.
Figure 13 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 13 Distribution map of species of Horologion. Black triangle: Horologion hubbardi, black circle: Horologion speokoites. Grey shaded areas represent exposed karst (Weary and Doctor 2014).
Figure 2 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 2 Horologion hubbardi female paratype, voucher CWH-452 (VMNH), dorsal habitus. Abdomen removed for DNA extraction. Scale bar: 1 mm.
Figure 11 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 11 Elytral characters of Horologion species AH. speokoites holotype, elytra, oblique left lateral aspect BH. hubbardi paratype (CMNH), ditto CH. hubbardi paratype (VMNH), SEM detail of apex of left elytron, ventral aspect DH. hubbardi paratype (VMNH), SEM photo of right elytron, dorsal aspect EH. hubbardi paratype (VMNH), SEM detail of right elytron (area indicated by black box in D). Black arrows: discal setae, white arrows: plica.
Figure 6 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 6 Support for and against previously proposed placements of Horologion. Grey: clade present in maximum likelihood tree, SBS less than 90%, UFBoot less than 95% and/or SH-aLRT less than 80. Red: clade absent in maximum likelihood tree, most-supported contradictory clade with SBS 90% or greater, UFBoot 95% or greater and SH-aLRT 80 or greater. Pink: clade absent in maximum likelihood tree, most-supported contradictory clade with SBS less than 50%, UFBoot less than 95% and/or SH-aLRT less than 80. There were not sufficient sequences available for COI and Topo from our sampled Psydrini.
Figure 14 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 14 Phylogenetic tree of a portion of Trechinae showing proposed synapomorphies of clades. Topology is that of Fig. 4.
Figure 10 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 10 Scanning electron microscope images of Horologion hubbardi female paratype, voucher CWH-452 (VMNH) A detail of right side of pterothorax, ventral aspect B mesoventrite, ventral aspect C metaventrite, ventral aspect, abdomen removed D detail of humeral region of right elytron, dorsal aspect.
Figure 4 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 4 Summary of the maximum likelihood tree of Trechinae from IQ-TREE analysis of 8-gene concatenated matrix. Standard bootstrap support values are shown below nodes. For complete phylogenetic tree showing details within each clade, see Suppl. material 4.
Supplementary material 4 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Trees from Maximum Likelihood and Bootstrap analyses of single gene and 8-gene matrices
Supplementary material 2 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Taxa sampled for phylogenetic analyses
Supplementary material 3 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Additional photos of Horologion and Bembidarenini
Supplementary material 1 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
PCR primers and protocols
Figure 4 from: Warsi W, Jaswir I, Ahmed QU, Mahfudh N, bin Mohd. Nawi MS, Rohman A, Khatib A (2024) Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos. Pharmacia 71: 1-10. https://doi.org/10.3897/pharmacia.71.e109918
Figure 4 Teratogenic effects of various doses of GsME in zebrafish embryos. A. Normal embryo; B. 250 mg/L; C. 30 mg/L; D. 125 mg/L; E. Normal control; F–I. 62.5 mg/L.
Figure 2 from: Warsi W, Jaswir I, Ahmed QU, Mahfudh N, bin Mohd. Nawi MS, Rohman A, Khatib A (2024) Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos. Pharmacia 71: 1-10. https://doi.org/10.3897/pharmacia.71.e109918
Figure 2 The LC50 and EC50 of GsME in zebrafish embryos: 1 dpf (A), 2 dpf (B), 3 dpf (C), 4 dpf (D).
Figure 1 from: Guo D, Engel MS, Shih C, Ren D (2024) New stenurothripid thrips from mid-Cretaceous Kachin amber (Thysanoptera, Stenurothripidae). ZooKeys 1192: 197-212. https://doi.org/10.3897/zookeys.1192.117754
Figure 1 Parallelothrips separatus gen. et sp. nov.; holotype male (CNU-THY-MA2016116) A dorsal view B ventral view C head and pronotum, dorsal view D left antenna, conical sensorium present on antennomeres III–IV.
Figure 3 from: Guo D, Engel MS, Shih C, Ren D (2024) New stenurothripid thrips from mid-Cretaceous Kachin amber (Thysanoptera, Stenurothripidae). ZooKeys 1192: 197-212. https://doi.org/10.3897/zookeys.1192.117754
Figure 3 Parallelothrips separatus gen. et sp. nov. A female (CNU-THY-MA2016117), dorsal view B fore leg of male (CNU-YHY-MA2016116), vesicle cone-shaped at the tip C mouth cone (CNU-THY-MA2016116), mp indicated: maxillary palpus, lp indicated: labial palpus D distal abdominal segments of male (CNU-THY-MA2016116), spine on segment IX (indicated by a white arrow).
Figure 5 from: Guo D, Engel MS, Shih C, Ren D (2024) New stenurothripid thrips from mid-Cretaceous Kachin amber (Thysanoptera, Stenurothripidae). ZooKeys 1192: 197-212. https://doi.org/10.3897/zookeys.1192.117754
Figure 5 Didymothrips abdominalis gen. et sp. nov. A holotype female (right) (CNU-THY-MA2016102/1) and paratype male (left) (CNU-THY-MA2016102/2), dorsal view B paratype female (CNU-THY-MA2016118), dorsal view C head, antenna and pronotum of CNU-THY-MA2016102/1 D forewing of CNU-THY-MA2016102/1.
Figure 4 from: Guo D, Engel MS, Shih C, Ren D (2024) New stenurothripid thrips from mid-Cretaceous Kachin amber (Thysanoptera, Stenurothripidae). ZooKeys 1192: 197-212. https://doi.org/10.3897/zookeys.1192.117754
Figure 4 Cycad pollen found in the CNU-THY-MA2016116 A pollen distributed around the thrips B pollen attached on the fringe cilia and legs C enlarged details of the cycad pollen.
Figure 2 from: Guo D, Engel MS, Shih C, Ren D (2024) New stenurothripid thrips from mid-Cretaceous Kachin amber (Thysanoptera, Stenurothripidae). ZooKeys 1192: 197-212. https://doi.org/10.3897/zookeys.1192.117754
Figure 2 Parallelothrips separatus gen. et sp. nov.; holotype male (CNU-THY-MA2016116) A right fore wing, dorsal view, the foremost fringe cilium lacks a corresponding anteromarginal setae (indicated by black arrows) B microtrichia of anterior wing margin C surface of hind wing, covered with microtrichia D ventral view of left antenna E antennomere V, showing rounded annulation sculpture and microtrichia F dorsal view of hind leg (from metafemur onward).
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