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Figure 13 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)

Figure 13. Secondary structure of the ITS2 molecule of hysterocinetids (A, B), Tetrahymena nigricans (C), Clausilocola apostropha (D), Tetrahymena nanneyi (E), and Tetrahymena foissneri (F). Arrows in (A–C) mark molecular synapomorphies of the 'paravorax' clade. Helices of the ITS2 molecule are marked by Roman numbers I–IV, the pyrimidine-pyrimidine mismatch of helix II is denoted by an orange rectangle.

opennotspecifiedJun 2023View details →
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Figure 12 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)

Figure 12. Secondary structure of the 5.8S rRNA molecule and the first two domains of the 28S rRNA molecule of Hysterocineta bellerophon. Arrows mark molecular synapomorphies of the 'paravorax' clade. The 5.8S-28S secondary structure map of Tetrahymena thermophila (inset) is from hưp://apollo.chemistry.gatech.edu/RibosomeGallery.

opennotspecifiedJun 2023View details →
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FIGURE 6. A, B in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 6. A, B. Drawing of Cothurnia buetschlii (specimen 2) ventrally on segment 10 of Setaphyes kielensis (ZMB 12486) as longitudinal optical section (A) and lorica in cross-section (B). Abbreviations: an, annulation on surface of zooid; en, endostyle with striae; inf, withdrawn infundibulum with cilia; lo, lorica; ma, macronucleus; mi, micronucleus; po, pore in cementing mass; sc, spherical cavity; st, stalk with internal striae; zo, zooid. Scale bar 30 µm.

opennotspecifiedSep 2023View details →
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FIGURE 4 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts. Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm.

opennotspecifiedSep 2023View details →
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FIGURE 8 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm.

opennotspecifiedSep 2023View details →
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FIGURE 3 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm.

opennotspecifiedSep 2023View details →
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FIGURE 10 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 10. Setaphyes kielensis (A–C, ZMB 12458; D, ZMB 12488) with cuticular marks and filamentous prokaryotes, SEM (A–C) and DIC (D). A. Cuticular marks (arrows) of detached ciliate specimens, laterodorsal view. B. Segment 1 with large mucous? attachment sites to basibiont (arrows), frontal view. C. Segments 7–11, ventral view. D. Segments 8–10, ventral view. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; pl, placid; s, segment, followed by segment number; su, suctorian epibiont. Scale bar in A–C 20 µm and in D 50 µm.

opennotspecifiedSep 2023View details →
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FIGURE 1 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 1. Map showing the locations of Pycnophyes communis, Setaphyes dentatus, and S. kielensis in European waters and the sampling site of S. dentatus and S. kielensis in the North Sea, island of Sylt (asterisks in inset A).

opennotspecifiedSep 2023View details →
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FIGURE 9 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 9. Setaphyes kielensis (ZMB 12486), segment 10 with Cothurnia buetschlii (specimens 1–4), ventral view. A. Overview of position of epibionts. Numbers indicate specimens of C. buetschlii (comp. also Table 6). B–G. Specimens 3 (B) and 4 (C–G), images 8 (C), 15 (D), 19 (E), 24 (F) and 28 (G) showing surface annulation of zooid (C, D), internal longitudinal striae of stalk and endostyle (D–F), and attachment site of stalk to the cuticle of the basibiont (G, arrowheads). Note elongate macronucleus and numerous micronuclei (E–G). White arrows (D, E) mark basal outer margin of lorica, black arrows (D, E) point to bottom margin of lorica. Abbreviations: an, annulation on surface of zooid; ci, ciliae; en, endostyle; in, withdrawn infundibulum; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; ps, penile spine; sc, spherical cavity; st, stalk; su, suctorian epibiont; zo, zooid. Scale bar in A 50 µm and in D 20 µm, valid for B–G.

opennotspecifiedSep 2023View details →
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FIGURE 7 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 7. Setaphyes kielensis (ZMB 12468) with epibionts, SEM. A. Segments 1–11, ventral-right. B. Segments 4–8, ventral view. Arrows mark laterally recessed anterior margin of lorica of Cothurnia buetschlii. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; tu, tube. Scale bar in A 100 µm and in B 20 µm.

opennotspecifiedSep 2023View details →
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FIGURE 2 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm.

opennotspecifiedSep 2023View details →
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FIGURE 5 in Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)

FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm.

opennotspecifiedSep 2023View details →
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FIGURE 2 in New record of Epistylis obliqua Sommer, 1951 (Ciliophora, Peritrichia) on aquatic mite Unionicola minor (Soar 1900) (Acari, Hydrachnidia)

FIGURE 2. Schematic drawings of Epistylis obliqua. A—after Sommer (1951); B—original. Scale bar: 50 µm.

opennotspecifiedOct 2023View details →
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FIGURE 1 in New record of Epistylis obliqua Sommer, 1951 (Ciliophora, Peritrichia) on aquatic mite Unionicola minor (Soar 1900) (Acari, Hydrachnidia)

FIGURE 1. Epistylis obliqua on Unionicola minor A—Habitus ventral B—Magnified dark field view of Epistylis obliqua colonies C—Magnified view of E. obliqua on the first leg of the mite. Scale bars: A = 200 µm, B= 100 µm, C= 50 µm.

opennotspecifiedOct 2023View details →
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Figure 8 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America

Figure 8. Maximum likelihood (ML) tree inferred from the small subunit rRNA gene sequences, revealing the positions of the 21 newly obtained sequences (red font). Numbers near branches indicate ML bootstrap values and BI posterior probabilities. Black circles represent full support (ML/BI, 100/1.00) in both analyses. Disagreements in topology between ML and BI are marked with asterisks. The scale bar corresponds to 2 changes per 100 nucleotide positions. Abbreviation: sp = species.

opennotspecifiedJul 2023View details →
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Figure 4 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America

Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations: CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C).

opennotspecifiedJul 2023View details →
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Figure 5 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America

Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape. C, extruded extrusomes (arrowheads). D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges. L, curved extrusomes (arrowheads) under the cortex. M, posterior end of cell, showing caudal cilia (arrowhead). N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm.

opennotspecifiedJul 2023View details →
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Figure 2 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America

Figure 2. Drawings of Trimyema foissneri from life (A–D) and aħer protargol staining (E–I). A, leħ lateral view of a representative individual, arrow shows acontractile vacuole, red arrowhead shows food vacuole, yellow arrowheads show somatic cilia. B, different body shape in different viewing angles. C, leħ lateral view, showing buccal cavity (arrow) and position of macronucleus. D, disposition of the ciliary girdles. E, oral ciliature, red dots showing kinetidal composition at the anterior part of OK1. F, lateral view of oral ciliature, showing the shorter oral kinety 3 (in red). G, macronucleus and micronucleus (arrowheads), the position of the laưer being variable (1, 2). H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrows show argentophilic lines connecting longitudinal somatic ciliary rows, arrowheads show epaulet. Abbreviations: CC, caudal cilia; Ma, macronucleus; OK, oral kinety; SK, somatic kineties; VLF, ventrolateral fragment. Scale bars: 15 μm (A, C); 10 μm (H, I).

opennotspecifiedJul 2023View details →
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Figure 6 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America

Figure 6. Drawings of Plagiopyla frontata from life (A–C, E, F) and after protargol staining (D, G–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, tangential section of the cortex, arrowhead marks straight extrusome, arrow indicates curved extrusome. C, extruded extrusomes with one end slightly inflated (red arrowheads). D, details of oral region, showing oral opening and buccal cavity (arrow). E, F, different cell shapes. G, dorsal view of a compressed cell, showing position of striated band, cytoproct (black arrow), dense ciliary rows (red arrow), and contractile vacuole pores (red arrowheads). H, ventral view to show somatic and oral kineties, arrowhead indicates gap between upper oral lip kineties and somatic kineties. I, dorsal view to show somatic kineties, arrow indicates striated band, arrowhead shows dense ciliary rows, double-arrowhead shows several irregularly and densely packed kinetosomes located near loop of striated band. Abbreviations: CV, contractile vacuole; Ma, macronucleus; SB, striated band. Scale bars: 35 μm (A, E, G, H); 10 μm (C).

opennotspecifiedJul 2023View details →
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Figure 7 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America

Figure 7. Photomicrographs of Plagiopyla flontata in vivo (A–H) and aħer protargol staining (I–M). A, ventral view of a slightly compressed cell, arrow marks buccal cavity. B, representative individual, arrow shows oral opening, arrowhead indicates contractile vacuole. C, extruded extrusomes with one end slightly inflated (arrowheads). D, contractile vacuole pores (red arrowheads). E, ventral view of a compressed cell, arrows show straight extrusomes, arrowheads show curved extrusomes. F, details of dorsal side, arrowhead shows cytoproct. G, right side of a compressed cell showing striated band (arrowhead). H, details of extrusomes. I, macronucleus and micronucleus (white arrowhead). J, K, oral region, showing buccal cavity under different focal planes. L, M, ventral (L) and dorsal (M) views of the same individual, showing somatic and oral kineties, red arrow indicates gap between upper oral lip kineties and somatic kineties, black arrow shows striated band, black arrowhead shows dense ciliary rows, red arrowhead indicates several polykineties located near loop of striated band. Abbreviation: Ma, macronucleus. Scale bars: 35 μm.

opennotspecifiedJul 2023View details →

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