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972 results for “morphological barcode”
Figure 10 from: Motamedinia B, Skevington JH, Kelso S (2019) Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes. ZooKeys 873: 85-111. https://doi.org/10.3897/zookeys.873.36645
Figure 10 Claraeola species distribution in the Middle East.
Data from: DNA barcoding gap: reliable species identification over morphological and geographical scales
The philosophical basis, and utility of DNA barcoding has been a subject of numerous debates. While most literature embraces it, some studies continue to question its use in dipterans, butterflies, and marine gastropods. Here, we explore the utility of DNA barcoding in identifying spider species that vary in taxonomic affiliation, morphological diagnosibility and geographic distribution. Our first test searched for a "barcoding gap" by comparing intra- and interspecific means, medians and overlap in more than 75,000 computed Kimura 2 parameter (K2P) genetic distances in three families. Our second test compared K2P distances of congeneric species with high versus low morphological distinctness in 20 genera of 11 families. Our third test explored the effect of enlarging geographical sampling area at a continental scale on genetic variability in DNA barcodes within 20 species of nine families. Our results generally point towards a high utility of DNA barcodes in identifying spider species. However, the size of the barcoding gap strongly depends on taxonomic groups and practices. It is becoming critical to define the barcoding gap statistically more consistently, and to document its variation over taxonomic scales. Our results support models of independent patterns of morphological and molecular evolution by showing that DNA barcodes are effective in species identification regardless of their morphological diagnosibility. We also show that DNA barcodes represent an effective tool for identifying spider species over geographic scales, yet their variation contains useful biogeographic information.
Fig. 14 in NGS-barcodes, haplotype networks combined to external morphology help to identify new species in the mangrove genus Ngirhaphium Evenhuis & Grootaert, 2002 (Diptera: Dolichopodidae: Rhaphiinae) in Southeast Asia
Fig. 14. Maximum likelihood tree of Ngirhaphium based on NGS barcodes (COI, 313bp).
Figure 2 from: Veijalainen A, Broad G, Wahlberg N, Longino J, Sääksjärvi I (2011) DNA barcoding and morphology reveal two common species in one: Pimpla molesta stat. rev. separated from P. croceipes (Hymenoptera, Ichneumonidae). ZooKeys 124: 59-70. https://doi.org/10.3897/zookeys.124.1780
Figure 2 - Pimpla molesta female, lateral view.
Figure 1 from: Veijalainen A, Broad G, Wahlberg N, Longino J, Sääksjärvi I (2011) DNA barcoding and morphology reveal two common species in one: Pimpla molesta stat. rev. separated from P. croceipes (Hymenoptera, Ichneumonidae). ZooKeys 124: 59-70. https://doi.org/10.3897/zookeys.124.1780
Figure 1 - Pimpla croceipes female, lateral view.
Figure 5 from: Stur E, Borkent A (2014) When DNA barcoding and morphology mesh: Ceratopogonidae diversity in Finnmark, Norway. ZooKeys 463: 95-131. https://doi.org/10.3897/zookeys.463.7964
Figure 5 - Female terminalia, in ventral view A Dasyhelea cf. malleola B Dasyhelea malleola.
Figure 3 from: Amora G, Hamada N, Fusari LM, Andrade-Souza V (2015) An Asiatic Chironomid in Brazil: morphology, DNA barcode and bionomics. ZooKeys 514: 129-144. https://doi.org/10.3897/zookeys.514.9925
Figure 3 - Chironomus striatipennis, Brazilian population. A Egg mass B Egg.
Data from: DNA barcoding gap: reliable species identification over morphological and geographical scales
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FIGURES 21–24 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 21–24. Ixchela zapatai sp. nov. Female epigynum: 21, ventral view. 22, dorsal view. 23, frontal view. 24, lateral view. MSE: median septum of epigynum, PP: pore plates. Scale bars: 0.5 mm.
FIGURES 10–13 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 10–13. Ixchela panchovillai sp. nov. Female epigynum: 10, ventral view. 11, dorsal view. 12, frontal view. 13, lateral view. MSE: median septum of epigynum, PP: pore plates. Scale bars: 0.5 mm (Figs 12, 13), 1 mm (Figs 10, 11).
FIGURE 2 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURE 2. Maximum Likelihood gene tree (highest log: -3749.87), constructed with COI barcode sequences of Ixchela. Colors in the branches indicate species; same colors are in the bars, which represent the different species delimitation methods used for their validation. Red branches + blue circles indicate the new species. Numbers below the bars represent the species recovered in each species delimitation method (not considering the outgroup: P. dugesi): 1: morphology (M). 2: neighbor-joining (NJ). 3: GMYC. 4: ABGD with recursive partitions (RP). 5: ABGD with initial partitions (IP). 6: bPTP with IB. 7: bPTP with ML. Numbers above branches are Bootstrap support values (>50% significant), number below are posterior probabilities (PP) support values under BI.
FIGURE 1 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURE 1. Neighbour-Joining (NJ) with p-distances tree constructed with COI barcode sequences from different specimens and species of Ixchela. Colors in the branches indicate species, red branches + blue circles indicate the new species. Male cheliceral, male palps, and female epigyna are showed for each species to show morphological structures taxonomically important at species level. Number on branches represent Bootstrap support values (>50% significant).
FIGURE 31 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURE 31. Type localities of Ixchela panchovillai sp. nov. (green circle) and Ixchela zapatai sp. nov. (red circle), in the state of Oaxaca, Mexico.
FIGURES 13–15 in Morphological description and DNA barcoding of some Diamesinae (Diptera Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic)
FIGURES 13–15. Arctodiamesa appendiculata (Lundström), male. 13, hypopygium in dorsal view; 14, inner structures of hypopygium; 15, distal part of gonostylus.
FIGURE 17 in Morphological description and DNA barcoding of some Diamesinae (Diptera Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic)
FIGURE 17. Bayesian tree based on mitochondrion COI gene for the genus Pseudokiefferiella Zavřel. SYM+G, F81 and HKY+G was the best models of nucleotide substitution for the first, second and third of COI codon position respectively. Bayesian posterior probabilities (PP) are given above tree nodes and bootstrap support values found in the ML analysis are shown below nodes. Specimens obtained in this study are in bold.
FIGURE 16 in Morphological description and DNA barcoding of some Diamesinae (Diptera Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic)
FIGURE 16. Bayesian tree based on mitochondrion COI gene for three species of the genus Arctodiamesa Makarchenko. K80, F81 and HKY+G was the best models of nucleotide substitution for the first, second and third of COI codon position respectively. Bayesian posterior probabilities (PP) are given above tree nodes and bootstrap support values found in the ML analysis are shown below nodes. Specimens obtained in this study are in bold.
FIGURES 1–7 in Morphological description and DNA barcoding of some Diamesinae (Diptera Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic)
FIGURES 1–7. Diamesa urvantsevi sp. nov. (1–4) and D. arctica (Boheman) (5–7), males. 1, 6, hypopygium in dorsal view; 2, gonostylus; 3–4, "anal point"; 5, inner structures of hypopygium; 6, distal part of gonostylus. Scale bar 20 μm.
FIGURES 8–12 in Morphological description and DNA barcoding of some Diamesinae (Diptera Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic)
FIGURES 8–12. Diamesa urvantsevi sp. nov. (8–10), D. amplexivirilia Hansen (11) and Pseudokiefferiella sp. (12), males. 8, 10–12, hypopygium in dorsal view; 9, "anal point". ap—"anal point".
FIGURE 32 in Review of the genus Shilovia Makarchenko (Diptera: Chironomidae: Diamesinae Boreoheptagyiini) from the mountains of Central Asia, with morphological description and DNA barcoding of known species
FIGURE 32. Bayesian tree based on mitochondrion COI gene for available members of the tribe Boreoheptagyiini. Bayesian posterior probabilities (PP) are given above tree nodes and bootstrap support values found in the ML analysis are shown below nodes. Specimens obtained in this study are in bold.
FIGURES 10–15 in Review of the genus Shilovia Makarchenko (Diptera: Chironomidae: Diamesinae Boreoheptagyiini) from the mountains of Central Asia, with morphological description and DNA barcoding of known species
FIGURES 10–15. Shilovia xinhuawangi sp. nov., male. 10, basal part of wing; 11, antenna; 12, dorsal part of mesonotum; 13, scutellum and postnotum; 14, hypopygium in dorsal view; 15, hypopygium without tergite IX in dorsal view.
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