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Figure 2 in Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2-methoxyethanol
Figure 2. Treatment group: 2- ME + Resveratrol Standard (10 x10).
Figure 1 in Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2-methoxyethanol
Figure 1. Control Group (10 x10).
Table 4 in Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2 - methoxyethanol
<p><b>Table 4.</b> Cytotoxic effect of various concentrations of Resveratrol and 2-Methoxyethanol Against Primary Neuron cell viability in MTT assay (570 nm).</p><table><tbody><tr><th><b>% Inhibition</b></th></tr></tbody><tbody><tr><th><b>Concentration of 2-Methoxyethanol</b></th><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td></tr><tr><th><b>and Resveratrol</b></th><td><b>Resveratrol (Standard)</b></td><td><b>Resveratrol (Tempeh)</b></td><td><b>Resveratrol (Soybean Seed Coat)</b></td></tr><tr><th>IC50</th><td>(17.38 ± 0.99 µg/mL)</td><td>(31.14 ± 0.02 µg/mL)</td><td>(13.40 ± 0.30 µg/mL)</td></tr><tr><th>1.4 (µg/mL)</th><td>12.57</td><td>0</td><td>11.98</td></tr><tr><th>2.8 (µg/mL)</th><td>4.59</td><td>1.75</td><td>17.03</td></tr><tr><th>4.2 (µg/mL)</th><td>0</td><td>0</td><td>0</td></tr></tbody></table>
Table 3 in Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2 - methoxyethanol
<p><b>Table 3.</b> Cytotoxic effect of various concentrations of Resveratrol and 2-Methoxyethanol Against Primary Neuron cell viability in MTT assay (540 nm).</p><table><tbody><tr><th><b>% Inhibition</b></th></tr></tbody><tbody><tr><th><b>Concentration of 2-Methoxyethanol</b></th><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td></tr><tr><th><b>and Resveratrol</b></th><td><b>Resveratrol (Standard)</b></td><td><b>Resveratrol (Tempeh)</b></td><td><b>Resveratrol (Soybean Seed Coat)</b></td></tr><tr><th>IC50</th><td>(17.64 ± 0.99 µg/mL)</td><td>(32.22 ± 1.18 µg/mL)</td><td>(17.43 ± 0.16 µg/mL)</td></tr><tr><th>1.4 (µg/mL)</th><td>11.8</td><td>0</td><td>7.95</td></tr><tr><th>2.8 (µg/mL)</th><td>5.27</td><td>0</td><td>14.41</td></tr><tr><th>4.2 (µg/mL)</th><td>0</td><td>0</td><td>0</td></tr></tbody></table>
Supporting information and Figures of the article "Harnessing the Biomimetic Effect of Macromolecular Crowding in the Cell-Derived Model of Clubfoot Fibrosis"
Open the record for dataset details and reuse information.
Table 2 in Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2 - methoxyethanol
<p><b>Table 2.</b> Effect of Various concentrations of Resveratrol and 2-Methoxyethanol against Primary Neuron cell viability (570 nm).</p><table><tbody><tr><th><b>% Cell Viability</b></th></tr></tbody><tbody><tr><th><b>Concentration of 2-Methoxyethanol</b></th><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td></tr><tr><th><b>and Resveratrol</b></th><td><b>Resveratrol (Standard)</b></td><td><b>Resveratrol (Tempeh)</b></td><td><b>Resveratrol (Soybean Seed Coat)</b></td></tr><tr><th>1.4 (µg/mL)</th><td>87.43</td><td>100</td><td>88.02</td></tr><tr><th>2.8 (µg/mL)</th><td>95.41</td><td>98.25</td><td>82.97</td></tr><tr><th>4.2 (µg/mL)</th><td>100</td><td>100</td><td>100</td></tr></tbody></table>
Table 1 in Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2 - methoxyethanol
<p><b>Table 1.</b> Effect of Various concentrations of Resveratrol and 2-Methoxyethanol against Primary Neuron cell viability (540 nm).</p><table><tbody><tr><th><b>% Cell Viability</b></th></tr></tbody><tbody><tr><th><b>Concentration of 2-Methoxyethanol</b></th><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td><td><b>2-ME (10mM)</b></td></tr><tr><th><b>and Resveratrol</b></th><td><b>Resveratrol (Standard)</b></td><td><b>Resveratrol (Tempeh)</b></td><td><b>Resveratrol (Soybean Seed Coat)</b></td></tr><tr><th>1.4 (µg/mL)</th><td>88.2</td><td>100</td><td>92.05</td></tr><tr><th>2.8 (µg/mL)</th><td>94.73</td><td>100</td><td>85.59</td></tr><tr><th>4.2 (µg/mL)</th><td>100</td><td>100</td><td>100</td></tr></tbody></table>
Efficient improvement of the proliferation, differentiation, and anti-arthritic capacity of mesenchymal stem cells by simply culturing on the immobilized FGF2 derived peptide, 44-ERGVVSIKGV-53
<p>All data needed to evaluate the results presented in the paper: Soo Bin Lee, Ahmed Abdal Dayem, Sebastian Kmiecik, Kyung Min Lim, Dong Sik Seo, Hyeong-Taek Kim, Polash Kumar Biswas, Minjae Do, Deok-Ho Kim, Ssang-Goo Cho, Efficient improvement of the proliferation, differentiation, and anti-arthritic capacity of mesenchymal stem cells by simply culturing on the immobilized FGF2 derived peptide, 44-ERGVVSIKGV-53,<br>Journal of Advanced Research, Volume 62,<br>2024,<br>Pages 119-141,<br>ISSN 2090-1232,<br>https://doi.org/10.1016/j.jare.2023.09.041.</p>
A transposase-derived gene responsible for human brain development
<p><span>Vertebrate brain development is associated with prominent neuronal cell death and DNA breaks, but their causes and functions are not well understood. DNA transposable elements and transposase-derived genes could contribute to DNA breaks and somatic genome rearrangements, however their contributions to brain development are largely unknown. PiggyBac Transposable Element Derived 5 (PGBD5) is an evolutionarily conserved vertebrate DNA transposase-derived gene with retained nuclease activity in human cells. Here, we show that PGBD5 contributes to normal brain development in mice and humans, and its deficiency causes disorder of intellectual disability, movement, and seizures. In mice, Pgbd5 is required for the developmental induction of post-mitotic DNA breaks and recurrent somatic brain<em> </em>genome rearrangements. In the cerebral cortex, loss of Pgbd5 leads to aberrant differentiation and gene expression of distinct neuronal populations, including specific types of glutamatergic neurons, which can explain the features of PGBD5 deficiency in humans. Thus, PGBD5 is a transposase-derived gene required for brain development in mammals. </span></p>
Planktonic foraminifera faunal data and derived sea-surface temperatures for the Marine Isotope Stage (MIS) 18 to MIS 28 interval of IODP Site U1387, Gulf of Cadiz
<p>Planktonic foraminifera faunal data and sea-surface temperature reconstructions for the early-middle Pleistocene interval between 750 and 1006 kilo years from IODP Site U1387 in the Gulf of Cadiz (southern Portuguese margin). This data was used to reconstruct the paleoecological and paleoclimatical changes at the southern Portuguese margin in Mega et al. (2025), The Early–Middle Pleistocene Transition in the Gulf of Cadiz (NE Atlantic) – an interplay between subtropical gyre and extremely cold surface waters. Clim. Past 21, 919-939, doi: 10.5194/cp-21-919-2025.</p> <p>The data is also available from the world data center Pangaea as a bundled data set:</p> <p>Voelker, Antje H L; Mega, Aline; Rodrigues, Teresa (2025): Planktonic foraminifera faunal data and sea-surface temperatures for the Marine Isotope Stage (MIS) 18 to MIS 28 interval of IODP Site 339-U1387, Gulf of Cadiz [dataset bundled publication]. PANGAEA, <a href="https://doi.org/10.1594/PANGAEA.974451" target="_blank" rel="nofollow noopener">https://doi.org/10.1594/PANGAEA.974451</a></p>
Annex B – Occurrence data on brominated phenols and their derivatives in food submitted to EFSA, dietary surveys per country and age group available in the EFSA Comprehensive Database considered in the exposure assessment, and the detailed results of the chronic dietary exposure assessment to 2,4,6-TBP and the contribution of different food groups to the dietary exposure
<p>This Annex contains the occurrence data submitted to EFSA, the dietary surveys per country and age group, and the detailed results of the chronic dietary exposure assessment to 2,4,6-TBP and the contribution of different food groups to the dietary exposure related to the Update of the risk assessment of brominated phenols and their derivatives in food.</p>
Dataset for the manuscript "Assessing the Role of Hydrodynamics in Enhancing HAND-Derived Synthetic Rating Curves: A Comparative Study in the Wu River Basin, Taiwan"
<p>This is the dataset for developing the HAND-hd workflow mentione in the manuscript "Assessing the Role of Hydrodynamics in Enhancing HAND-Derived Synthetic Rating Curves: A Comparative Study in the Wu River Basin, Taiwan". In the manuscript, we used the topographical cross-sectional survey data to generate topographical synthetic rating curves (RCb) to validate the performance of the HAND method based synthetic rating curves (HAND-SRC) produced by the HAND-hd workflow. Due to regulatory restrictions under Taiwanese law, public sharing of 5-meter (or finer) DEM data is prohibited. However, researchers interested in accessing processed HAND raster data for research purposes may contact the corresponding author.</p>
Primary data for manuscript provisionally titled, "Fluorination-homologation of the biomass derivative Cyrene"
<p>Primary NMR FID files and HRMS data collected for compounds described in the manuscript, "Fluorination-homologation of the biomass derivative Cyrene."</p> <p>This material can be opened and processed using Topspin or Spinworks programs. </p>
Agreement of multiple night- and daytime filtering approaches of eddy covariance-derived net ecosystem CO2 exchange over a mountain forest. Reproducible workflow.
<p>Datasets and python scripts to reproduce results from the publication <em>Agreement of multiple night- and daytime filtering approaches of eddy covariance-derived net ecosystem CO2 exchange over a mountain forest.</em></p> <p>See README.txt for a description of the single files.</p>
Four-Step Access to the Sesquiterpene Natural Product Presilphiperfolan-1β-ol and Unnatural Derivatives via Supramolecular Catalysis
<p>Data underlying the figures in the publication “Four-Step Access to the Sesquiterpene Natural Product Presilphiperfolan-1β-ol and Unnatural Derivatives via Supramolecular Catalysis”, published in <em>ChemCatChem,</em> <strong>2020</strong>, 12, 4512–4516. <a href="https://pubs.acs.org/doi/10.1021/jacs.0c01464">https://pubs.acs.org/doi/10.1021/jacs.0c01464</a></p> <p>Table of contents:</p> <p><strong>1. Crystals</strong>; Word file with the crystallographic data for compounds <strong>4</strong> and <strong>28</strong>.</p> <p><strong>2. Crystal_4</strong>; <em>.cif</em> file for crystal structure of compound <strong>4</strong>.</p> <p><strong>3. Crystal_28</strong>; <em>.cif</em> file for crystal structure of compound <strong>28</strong>.</p> <p><strong>4. Figure_2_NMRs</strong>; MestReNova file containing NMR spectra for compounds in <em>Figure 2.</em></p> <p><strong>5. Figure2_3_GCs</strong>; Zip file containing ASCII text raw data for GC traces relevant to <em>Figures 2</em> and <em>3</em>.</p> <p><strong>6. Figure4</strong>; Word file with the computational data for <em>Figure 4.</em></p> <p><strong>7. Procedures</strong>; Word file with the procedures for <em>Schemes 2 & 3 </em>and<em> Figures 2 & 3.</em></p> <p><strong>8. Scheme_2_NMRs</strong>; MestReNova file containing NMR spectra for compounds in<em> Scheme 2</em>.</p> <p><strong>9. Scheme3_NMRs</strong>; Zip file containing NMR spectra (as .mnova files) for <em>Scheme 3</em>.</p> <p> </p>
Delta-15N values for leaf and soil samples from a mesocosm experiment looking at dung beetle presence and the movement of dung-derived nitrogen (DDN)
<b>Description: </b><p>We deployed 18 mesocosms into each of the ecosystem types (logged forest and oil palm) in mid-May 2016, to give the soil one month to recover from the disturbance. We constructed mesocosms from black plastic containers, 40 cm diameter and 25 cm high after removing the base. We dug mesocosms 20 cm into the ground leaving 5cm above the surface. We arranged them in a 6 x 3 grid with a minimum of 3 m between each mesocosm to minimise interaction between the soil nutrient cycling in each mesocosm. As proximity of the seedlings to mature trees may increase competition for nitrogen and other nutrients we recorded the distance of each mesocosm to the nearest mature tree (any species with diameter at breast height > 30 cm) for inclusion in our analyses. <br>We randomly selected 12 of the mesocosms, to receive 15N-labelled dung patties weighing 300 ± SD 2.27 g in logged forest and 410 ± SD 2.04 g in oil palm. We populated six randomly selected mesocoms from within those 12 treated with dung with a standardised dung beetle communities (Fig. 1, Table S2). The remaining six mesocosms were left as soil only controls. We covered each mesocosm with a fine nylon mesh secured with a rubber belt to prevent beetles leaving or colonising the mesocosms, and to standardise any microclimatic effects between treatments. However, after 48 hours we opened the dung beetle treatments for a 24 hours period to allow the beetles to emigrate rather than forcing them to artificially stay in the same pat (cf. Roslin 2000; Slade et al. 2017), and then re-covered the mesocosms with netting.<br>We sampled soil seven times from logged forest over the course of the experiment. Any remaining surface dung was removed prior to soil sampling, and replaced thereafter, in order to reduce the possibility of contamination. If a soil core was unsuccessful (most likely due to beetle channels) a second core was taken directly beside. On each sample day, a core of 10cm depth was taken and split into vertical horizons 0- 2cm, 2-5 cm and 5-10cm. <br>We sampled leaves eight times over the eight-month duration of the experiment, with high frequency during the first month, aimed to capture the initial assimilation of DDN into the plants. We collected one leaf from the Dipterocarpaceae or palm seedlings for each sample event. For dipterocarp seedlings we alternated collection of the terminal leaf from top and bottom (leaving the topmost, newest leaf) between consecutive sample days, and for palm seedlings we sampled the two penultimate leaflets from alternating sides of the mid-stem, from the youngest fully formed frond. As assimilated 15N did not plateau in logged forest during the 8-month timeframe of the experiment, we took a sample after 21 months in order to determine whether all DDN had been turned over in the plant biomass after this time.<br>The leaf and soil samples were dried at 60°C for a minimum of 48 hours. We then ground samples to a fine powder using a ball mill (Retsch UK Ltd., Hope, UK). We weighed ground samples into 6 x 4 mm ultraclean tin capsules (Elemental Microanalysis Ltd., Okehampton, UK) using an ultra-microbalance with readability 1 μg (Mettler-Toledo, Greifensee, Switzerland) to provide sufficient elemental carbon and nitrogen for analysis by continuous flow isotope ratio mass spectrometry (SERCON, Crewe, UK). <br>Isotope ratios are expressed in per mil (‰) relative to international reference standards (Rstandard), which are Atmospheric Nitrogen and Vienna PeeDee Belemnite (VPDB) for nitrogen and carbon, respectively. The delta value describes the isotopic composition of each sample, which signifies a measurement of difference relative to laboratory standards. The calculation of δ values is given by: <br>δHX = [(RSAMPLE /RSTANDARD −1)]*1000</p><p><b>Project: </b>This dataset was collected as part of the following SAFE research project: <a href="https://www.safeproject.net/projects/project_view/11"><b>Using stable isotopes to link biogeochemical processes to biodiversity of conservation concern</b></a></p><p><b>Funding: </b>These data were collected as part of research funded by: </p><ul><li>NERC (Research grant, NE/K016148/1)</li></ul><p>This dataset is released under the CC-BY 4.0 licence, requiring that you cite the dataset in any outputs, but has the additional condition that you acknowledge the contribution of these funders in any outputs.</p><p></p><p><b>Permits: </b>These data were collected under permit from the following authorities:</p><ul><li>Sabah Biodiversity Centre (SABC) (Research licence JKM/MBS.1000-2/2 (374) )</li><li>Sabah Biodiversity Centre (SABC) (Research licence JKM/MBS.1000-2/2 JLD.4 (41))</li><li>Sabah Biodiversity Centre (SABC) (Research licence JKM.1000-2/2 JLD.5 (153))</li></ul><p></p><p><b>XML metadata: </b>GEMINI compliant metadata for this dataset is available <a href="https://www.safeproject.net/datasets/xml_metadata?id=5113431">here</a></p><p><b>Files: </b>This consists of 1 file: 3_Kemp_15N_mesocosms_data.xlsx</p><p><b>3_Kemp_15N_mesocosms_data.xlsx</b></p><p>This file contains dataset metadata and 3 data tables:</p><ol><li><p><b>OP leaf</b> (described in worksheet OP_leaf)</p><p>Description: Details the δ15N of leaves sampled in oil palm from palm seedlings across eight sample days up to day 233. </p><p>Number of fields: 13</p><p>Number of data rows: 144</p><p>Fields: </p><ul><li><b>Name</b>: Code for date (ddmm), mesocosms ID and depth (00 = surface; 02 = 2 cm belowground; 05 = 5 cm belowground, and 10 = 10 cm belowground) (Field type: id)</li><li><b>Day</b>: Experimental day as the number of days since day zero (defined by the planting of the seedlings, either dipterocarps or palms) (Field type: id)</li><li><b>Day2</b>: Experimental day as a factor (Field type: id)</li><li><b>Mesocosm</b>: Unique identifier for each of the 18 mesocosms (Field type: id)</li><li><b>Treatment</b>: Treatment assignment (Field type: categorical)</li><li><b>DistMature</b>: Distance from the nearest mature tree (any species with diameter at breast height > 30 cm). (Field type: numeric)</li><li><b>Weight</b>: Sample weight (Field type: numeric)</li><li><b>Beam.Area.N</b>: Measure of the nitrogen peak i.e. calculated as the area under the nitrogen curve by Calisto software. This value is directly related to N_weight. (Field type: numeric)</li><li><b>ugN</b>: Measure of the elemental nitrogen content of the sample (Field type: numeric)</li><li><b>d15N</b>: the delta value of the sample, which describes the ration of 15N: 14N isotopes (Field type: numeric)</li><li><b>Beam.Area.C</b>: Measure of the carbon peak i.e. calculated as the area under the carbon curve by Calisto software. This value is directly related to C_weight (Field type: numeric)</li><li><b>ugC</b>: Measure of the elemental carbon content of the sample (Field type: numeric)</li><li><b>d13C</b>: the delta value of the sample, which describes the ration of 13C: 12C isotopes (Field type: numeric)</li></ul></li><li><p><b>LFE leaf</b> (described in worksheet LFE_leaf)</p><p>Description: Details the δ15N of leaves sampled in logged forest, taken from dipterocarp seedlings across nine sample days, up to day 625.</p><p>Number of fields: 13</p><p>Number of data rows: 147</p><p>Fields: </p><ul><li><b>Name</b>: Code for date (ddmm), mesocosms ID and depth (00 = surface; 02 = 2 cm belowground; 05 = 5 cm belowground, and 10 = 10 cm belowground) (Field type: id)</li><li><b>Day</b>: Experimental day as the number of days since day zero (defined by the planting of the seedlings, either dipterocarps or palms) (Field type: id)</li><li><b>Day2</b>: Experimental day as a factor (Field type: id)</li><li><b>Mesocosm</b>: Unique identifier for each of the 18 mesocosms (Field type: id)</li><li><b>Treatment</b>: Treatment assignment (Field type: categorical)</li><li><b>DistMature</b>: Distance from the nearest mature tree (any species with diameter at breast height > 30 cm). (Field type: numeric)</li><li><b>Weight</b>: Sample weight (Field type: numeric)</li><li><b>Beam.Area.N</b>: Measure of the nitrogen peak i.e. calculated as the area under the nitrogen curve by Calisto software. This value is directly related to N_weight. (Field type: numeric)</li><li><b>ugN</b>: Measure of the elemental nitrogen content of the sample (Field type: numeric)</li><li><b>d15N</b>: the delta value of the sample, which describes the ration of 15N: 14N isotopes (Field type: numeric)</li><li><b>Beam.Area.C</b>: Measure of the carbon peak i.e. calculated as the area under the carbon curve by Calisto software. This value is directly related to C_weight (Field type: numeric)</li><li><b>ugC</b>: Measure of the elemental carbon content of the sample (Field type: numeric)</li><li><b>d13C</b>: the delta value of the sample, which describes the ration of 13C: 12C isotopes (Field type: numeric)</li></ul></li><li><p><b>LFE soil</b> (described in worksheet LFE_soil)</p><p>Description: Details the δ15N of soil sampled in logged forest across seven sample days up to day 64</p><p>Number of fields: 14</p><p>Number of data rows: 375</p><p>Fields: </p><ul><li><b>Name</b>: Code for date (ddmm), mesocosms ID and depth (00 = surface; 02 = 2 cm belowground; 05 = 5 cm belowground, and 10 = 10 cm belowground) (Field type: id)</li><li><b>Day</b>: Experimental day as the number of days since day zero (defined by the planting of the seedlings, either dipterocarps or palms) (Field type: id)</li><li><b>day2</b>: Experimental day as a factor (Field type: id)</li><li><b>Mesocosm</b>: Unique identifier for each of the 18 mesocosms (Field type: id)</li><li><b>Treatment</b>: Treatment assignment (Field type: categorical)</li><li><b>DistMature</b>: Distance from the nearest mature tree (any species with diameter at breast height > 30 cm). (Field type: numeric)</li><li><b>Depth</b>: The depth which the soil sample was taken from, i.e. 0002 is the horizon between the ground surface and 2 cm belowground (Field type: numeric)</li><li><b>Weight</b>: Sample weight (Field type: numeric)</li><li><b>Beam.Area.N</b>: Measure of the nitrogen peak i.e. calculated as the area under the nitrogen curve by Calisto software. This value is directly related to N_weight. (Field type: numeric)</li><li><b>ugN</b>: Measure of the elemental nitrogen content of the sample (Field type: numeric)</li><li><b>d15N</b>: the delta value of the sample, which describes the ration of 15N: 14N isotopes (Field type: numeric)</li><li><b>Beam.Area.C</b>: Measure of the carbon peak i.e. calculated as the area under the carbon curve by Calisto software. This value is directly related to C_weight (Field type: numeric)</li><li><b>ugC</b>: Measure of the elemental carbon content of the sample (Field type: numeric)</li><li><b>d13C</b>: the delta value of the sample, which describes the ration of 13C: 12C isotopes (Field type: numeric)</li></ul></li></ol><p><b>Date range: </b>2016-05-01 to 2017-02-01</p><p><b>Latitudinal extent: </b>4.5000 to 5.0700</p><p><b>Longitudinal extent: </b>116.7500 to 117.8200</p>
Innate immune activation by checkpoint inhibition in patient-derived lung cancer tissues
<p><span>Although Pembrolizumab-based immunotherapy has significantly improved lung cancer patient survival, many patients show variable efficacy and resistance development. A better understanding of the drug's action is needed to improve patient outcomes. Functional heterogeneity of the tumor microenvironment (TME) is crucial to modulating drug resistance; understanding of individual patients' TME that impacts drug response is hampered by lack of appropriate models. </span></p> <p><span>Lung organotypic tissue slice cultures (OTC) with patients' native TME procured from primary and brain-metastasized (BM) non-small cell lung cancer (NSCLC) patients were treated with Pembrolizumab and/or beta-glucan (WGP, an innate immune activator). Metabolic tracing with<sup> 13</sup>C<sub>6</sub>-Glc/<sup>13</sup>C<sub>5,</sub><sup>15</sup>N<sub>2</sub>-Gln, multiplex immunofluorescence (mIF), and digital spatial profiling (DSP) were employed to interrogate metabolic and functional responses to Pembrolizumab and/or WGP.</span></p> <p><span>Primary and BM PD-1<sup>+</sup> lung cancer OTC responded to Pembrolizumab and Pembrolizumab + WGP treatments, respectively. Pembrolizumab activated innate immune metabolism and functions in primary OTC, which were accompanied by tissue damage. DSP analysis indicated an overall decrease in immunosuppressive macrophages and T cells but revealed microheterogeneity in immune responses and tissue damage. Two TMEs with altered cancer cell properties showed resistance. Pembrolizumab or WGP alone had negligible effects on BM-lung cancer OTC but Pembrolizumab + WGP blocked central metabolism with increased pro-inflammatory effector release and tissue damage.</span></p> <p><span>In depth metabolic analysis and multiplex TME imaging of lung cancer OTC demonstrated overall innate immune activation by Pembrolizumab but heterogeneous responses in the native TME of a patient with primary NSCLC. Metabolic and functional analysis also revealed synergistic action of Pembrolizumab and WGP in OTC of metastatic NSCLC.</span></p>
Fig. 2. IGM 100 in A Small Derived Theropod from Öösh, Early Cretaceous, Baykhangor Mongolia
Fig. 2. IGM 100/1119 in right lateral view. Anatomical labels in appendix 3.
FIGURE 7 in Reconstruction Of Stem Species Pattern As A Strategy Towards Integrated Phylogenetic Systematics And Taxonomy, Applied To Early-Derivative Poronota (Oribatida)
FIGURE 7: Cladogram with selected characters and species of Oribatellidae. – Explanations see figure 4.
FIGURE 6 in Reconstruction Of Stem Species Pattern As A Strategy Towards Integrated Phylogenetic Systematics And Taxonomy, Applied To Early-Derivative Poronota (Oribatida)
FIGURE 6: Cladogram with selected characters and species of Achipteriidae. – Explanations see figure 4.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.