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143 results for “DNA extract”
Supplementary material 5 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871
COI MOTU table
Supplementary material 3 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871
16S reference library
Supplementary material 8 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871
Lysis buffers and purification protocols
Supplementary material 4 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871
Bioinformatic pipeline
Supplementary material 2 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871
COI reference library
Figure 6 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 6 - Habitus of Parabathynellidae family: Paraiberobathynella cf. fagei (Delamare Deboutteville & Angelier, 1950) from Higuera cave, Murcia (Spain). Lateral view.
Figure 7 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 7 - Permanent slides (special metal slides) of holotypes of the MNCN collections. Mounting medium: glycerine gelatin stained with methylene blue.
Figure 5 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 5 - Number of species by families and continents present in the MNCN collections versus world.
Figure 9 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 9 - Number of species (total and DNA extract) of Bathynellacea by families in the MNCN collections.
Figure 4 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 4 - Number of genera of Bathynellacea by continents present in the MNCN collections versus world.
Figure 14 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 14 - Number of records of Bathynellacea from Spain by Autonomous Communities in the MNCN collections.
Figure 8 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 8 - Number of genera of Bathynellacea by families and continents in the MNCN collections versus world.
Figure 11 from: Camacho AI, Dorda BA, Chillón BS, Rey I (2017) The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract. ZooKeys 678: 31-63. https://doi.org/10.3897/zookeys.678.11543
Figure 11 - Number of holotypes (DNA extract) of Bathynellacea by continents and families in the MNCN collections.
Fig. 3 in Protocol Optimization For Genomic Dna Extraction And Rapd-Pcr Of Alien Ponto-Caspian Amphipod Pontogammarus Robustoides
Fig. 3. RAPD fingerprints results from different samples of Pontogammarus robustoides with primers OPA-02 (M- marker, 1-15 runners- different samples of Pontogammarus robustoides; K- control) using RAPD-PCR 10 × Taq buffer with (NH4)2SO4.
Supplementary material 2 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Final species table. :
Supplementary material 5 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Methods (Scripts) :
Supplementary material 3 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Number of reads per sample. :
Supplementary material 4 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Diversity analyses. :
Supplementary material 1 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Fragment and working PCR conditions. :
Supplementary material 5 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Alternative statistical model :
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International Brain Laboratory public data
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OpenNeuro
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