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95 results for “Glucosinolates”
RNA-seq analysis of transcriptome and glucosinolate metabolism in seeds and sprouts of broccoli (Brassica oleracea var. italica)
GEO Series GSE53298. Brassica oleracea var. italica. 5 samples. Type: Expression profiling by high throughput sequencing.
Fig. 8 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 8. The intraspecific diversity of Barbarea vulgaris and relations to other species in the genus analyzed by parsimony network analysis of ITS regions from Barbarea accessions using SplitsTree (Huson and Briant, 2006). The B. vulgaris ITS sequences are defined as seven groups and detailed accession information is found in Supplementary Table S1. The respective alignment is shown in Supplementary Table S2, and bootstrap values are provided for 1000 replicates.
Fig. 1. A- Ochradenus baccatus plant. B– O in Investigation of glucosinolates in the desert plant Ochradenus baccatus (Brassicales: Resedaceae). Unveiling glucoochradenin, a new arabinosylated glucosinolate
Fig. 1. A- Ochradenus baccatus plant. B– O. baccatus root (Plant #7; scale bar = 10 cm). C- Semi-preparative RP-HPLC profile of desulfated GSLs extracted from O. baccatus root (1b, RT = 10.9; 2b, RT = 12.0; 3b, RT = 12.9; 4b, RT = 13.5; 5b, RT = 14.7; 6b, RT = 16.6). For representative quantitative HPLC chromatogram see Figure S35.
Fig. 18 in Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants
Fig. 18. Some proposed glucosinolates (GSLs) in need of more evidence. (A) Two GSLs suggested from MS to be isomers of [3], but without general NMR evidence or published MS/MS evidence for the position of the acetyl groups at the rhamnose residue, as opposed to the glucose residue or another isomer. (B) 2-Hydroxy-8-(methylsulfinyl)octyl GSL suggested from MS/MS evidence but without NMR evidence.
Fig. 13 in Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants
Fig. 13. The usefulness of the COSY spectrum for establishing connectivity, and an example of NMR of an intact glucosinolate (GSL). (A) The 1H NMR spectrum of 3-(hydroxymethyl)methylpentyl GSL (141) in D2O. A major impurity of acetate from the ion exchange isolation is seen. (B) The corresponding COSY spectrum. Signals along the diagonal correspond to the 1D spectrum. Whenever protons are found at the same or neighboring carbons, a "cross peak" can be seen with the x-coordinate of the one signal and the y-coordinate of the other. Original data by the authors (CEO and NA, Sections 6.1 and 6.2).
Fig. 6 in Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants
Fig. 6. Biosynthesis of six Met-derived glucosinolates (GSLs) in Arabidopsis thaliana starting with chain elongation (8 enzymatic steps for increasing the number of C atoms with two), followed by core GSL biosynthesis leading to the parent dihomoMet derived GSL 84, 4-(methylsulfanyl)butyl GSL. Further sequential secondary modifications of the parent GSL, via 4-(methylsulfinyl)butyl GSL (64) and but-3-enyl GSL (12) ends with 2-hydroxybut-3-enyl GSL (mixture of two stereoisomers, 24R and 24S, in this species) (Sønderby et al., 2010a). A different route leads from 64 to the alcohol [26] and the benzoyl ester 5 (Lee et al., 2012).
Fig. 7 summarizes a in Glucosinolate catabolism during postharvest drying determines the ratio of bioactive macamides to deaminated benzenoids in Lepidium meyenii (maca) root flour
Fig. 7 summarizes a proposed metabolic scheme for the drying process based on the findings presented here. We have divided it in steps, A through F, that group metabolic reactions taking place at different stages
Fig. 1 in Glucosinolate catabolism during postharvest drying determines the ratio of bioactive macamides to deaminated benzenoids in Lepidium meyenii (maca) root flour
Fig. 1. Benzyl glucosinolate and hydrolytic postharvest metabolites mentioned in the text. For macamides R = palmitic acid (9), stearic acid (10), oleic acid (11), linoleic acid (12), linolenic acid (13).
Simulated Galactic Cosmic Ray Exposure Activates Dose-Dependent DNA Repair Response and Downregulates Glucosinolate Pathway in Arabidopsis Seedlings
This study's objective was to develop an understanding of the biological effects of space radiation on plants with the goal of producing fresh food during long duration space missions to support astronauts' nutritional and psychological needs.10-day-old Arabidopsis seedlings were exposed to simulated Galactic Cosmic Rays (GCR) and assessed for transcriptomic changes. The simulated GCR irradiation was carried out in the NASA Space Radiation Laboratory (NSRL) at Brookhaven National Lab (BNL). The exposures were conducted acutely for two dose points at 40 cGy or 80 cGy, with sequential delivery of proton, helium, oxygen, silicon, and iron ions. Control and irradiated seedlings were then harvested and stabilized in RNAlater at 3 hrs. post irradiation. Total RNA was isolated for transcriptomic analyses using RNAseq. The data revealed that the transcriptomic responses were dose-dependent, with significant upregulation of DNA repair pathways and downregulation of glucosinolate biosynthetic pathways. Glucosinolates are important for plant pathogen defense and for the taste of a plant, which are both relevant to growing plants for spaceflight. These findings fill in knowledge gaps of how plants respond to radiation in beyond-Earth environments.
Overexpression of ENOD40 in Arabidopsis thaliana plants reveals a role of the gene in cell wall turnover and glucosinolate accumulation.
GEO Series GSE37867. Arabidopsis thaliana. 12 samples. Type: Expression profiling by array.
Effect of nitrogen compounds on imbibed mutant seeds impaired in glucosinolate biosynthesis
GEO Series GSE221567. Arabidopsis thaliana. 45 samples. Type: Expression profiling by high throughput sequencing.
Fig. 8 in Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants
Fig. 8. Examples of the diversity of glucosinolate breakdown products. MYR, myrosinase.
Transcriptome and QTL mapping analyses identifying major QTL genes controlling glucosinolates contents between vegetable-type and oilseed-type Brassica rapa plants
GEO Series GSE213605. Brassica rapa. 12 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome Analysis of Arabidopsis thaliana and Changes of Glucosinolates Metabolism Pathway Induced by Flg22
GEO Series GSE56117. Arabidopsis thaliana. 2 samples. Type: Expression profiling by high throughput sequencing.
A light signaling factor, ELONGATED HYPOCOTYL 5 (HY5) interacts with Histone deacetylase 9 (HDA9) to play a suppressive role in the glucosinolate biosynthesis in Arabidopsis
GEO Series GSE250418. Arabidopsis thaliana. 6 samples. Type: Expression profiling by high throughput sequencing.
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