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185 results for “Multigene”
FIGURE 1 in Multigene phylogeny and morphology reveal Cytospora spiraeae sp. nov. (Diaporthales, Ascomycota) in China
FIGURE 1. Phylogram of Cytospora based on ITS gene. MP and ML bootstrap support values above 50 % are shown at the first and second position. Thickened branches represent posterior probabilities above 0.95 from BI. Ex-type strains are in bold. Strains in current study are in blue.
FIGURE 2 in Multigene phylogeny and morphology of Alternaria reveal a novel species and a new record in China
FIGURE 2. Morphology of Alternaria carotiincultae YZU 151039 from Ligusticum chuanxiong. A: colony on PDA. B–D: sporulations on PCA. E: conidia. Scale bars = 20 mm.
FIGURE 1 in Multigene phylogeny and morphology of Alternaria reveal a novel species and a new record in China
FIGURE 1. ML phylogram of the present Alternaria strains (in bold) based on combined ITS, EF-1α, RPB2 and ATPase gene sequences. Bootstrap support values above 70 % from 1000 replicates are shown at the nodes. The bar indicates the number of substitutions per position.
FIGURE 3 in Multigene phylogeny and morphology of Alternaria reveal a novel species and a new record in China
FIGURE 3. Morphology of Alternaria glehniae YZU 161149 from Glehnia littoralis. A: colony on PDA. B, C: sporulations on PCA. D: conidia. Scale bars = 20 mm.
FIGURE 2 in Introducing the new Indian mangrove species, Vaginatispora microarmatispora (Lophiostomataceae) based on morphology and multigene phylogenetic analysis
FIGURE 2. Vaginatispora microarmatispora (holotype). a–b. Ascomata immersed, aggregated, erumpent on the dead wood of Aegiceras corniculatum. c–d. Longitudinal sections of ascomata e. Ostiole with periphyses. f. Section of peridium comprising inner hyaline to pale brown cells of textura angularis. g. Cellular and hyaline pseudoparaphyses. h–j, l. Immature and matured asci. k. Ascospore with an indistinct mucilaginous sheath and distinct hyaline appendages. m–p. Ascospores with large guttules. o. Ascospore stained in Indian ink. q. Germ tubes developed from terminal ends of ascospore. Scale bars: c–d = 100 μm, e, j, l = 50 μm, f–h, k, m–q = 10 μm.
FIGURE 1 in Introducing the new Indian mangrove species, Vaginatispora microarmatispora (Lophiostomataceae) based on morphology and multigene phylogenetic analysis
FIGURE 1. RAxML tree based on analysis of a combined dataset of LSU, SSU, ITS and TEF partial sequence data. Bootstrap support values for ML, MP higher than 75 % and BYPP greater than 0.95 are given above each branch respectively. The new isolate is in blue. The tree is rooted to Angustimassarina populi (Amorosiaceae).
Figure 8 in Re-discovery and novel contributions to morphology and multigene phylogeny of Myxophyllum steenstrupi (Ciliophora: Pleuronematida), an obligate symbiont of terrestrial pulmonates
Figure 8. Phylogenetic tree based on the nuclear 18S rRNA gene as well as on the mitochondrial COI and 16S rRNA gene sequences, showing the systematic position of M. steenstrupi isolated from the plaited door snail Cochlodina laminata. The class Colpodea was used as the outgroup. Bootstrap values for maximum likelihood conducted in IQTrees as well as posterior probabilities for Bayesian inferences conducted in MrBayes and Phycas were mapped onto the best scoring IQ tree. Dash indicates mismatch between maximum likelihood and Bayesian tree topologies. Sequences marked in red were obtained during this study. Fully statistically supported nodes are marked with red solid circles. GenBank accession numbers can be found in Supporting Information (Table S6). The scale bar denotes six substitutions per one hundred nucleotide positions.
Figure 6 in Re-discovery and novel contributions to morphology and multigene phylogeny of Myxophyllum steenstrupi (Ciliophora: Pleuronematida), an obligate symbiont of terrestrial pulmonates
Figure 6. Phylogenetic tree based on the nuclear 18S, 5.8S, and 28S rRNA gene sequences, showing the systematic position of M. steenstrupi isolated from the plaited door snail Cochlodina laminata. The subclass Scuticociliatia is represented by the orders Philasterida and Pleuronematida, the subclass Astomatia was used as the outgroup. Bootstrap values for maximum likelihood conducted in IQTrees as well as posterior probabilities for Bayesian inferences conducted in MrBayes and Phycas were mapped onto the best scoring IQ tree. Dash indicates mismatch between maximum likelihood and Bayesian tree topologies. Sequences marked in red were obtained during this study. Fully statistically supported nodes are marked with red solid circles. GenBank accession numbers can be found in Supporting Information (Table S2). The scale bar denotes seven substitutions per one hundred nucleotide positions.
Figure 2. M. steenstrupi after protargol impregnation. A, B in Re-discovery and novel contributions to morphology and multigene phylogeny of Myxophyllum steenstrupi (Ciliophora: Pleuronematida), an obligate symbiont of terrestrial pulmonates
Figure 2. M. steenstrupi after protargol impregnation. A, B, top and bottom overviews of the same specimen, showing the ciliary pattern and nuclear apparatus. Black arrowheads mark the anterior suture, white double arrowheads denote the right lateral suture, white arrowheads denote the left lateral suture, and arrows mark the posterior suture. C, detail of the silverline system in the anterior body portion. D, semischematic diagram, showing the ciliary pattern within and around the mouth pocket. MA, macronuclear nodules; MI, micronucleus; PM, paroral membrane; SK, somatic kineties; SL, silverline; VK, vestibular kineties. Scale bars = 20 Μm (C), 55 Μm (A, B).
Figure 4. M in Re-discovery and novel contributions to morphology and multigene phylogeny of Myxophyllum steenstrupi (Ciliophora: Pleuronematida), an obligate symbiont of terrestrial pulmonates
Figure 4. M. steenstrupi after protargol impregnation (A–D, H) and after silver nitrate impregnation (E–G). A, detail of the anterolateral body region, showing the anterior suture (black arrowhead) made by abutting anterior ends of ventral kineties and thigmotactic kineties. B, detail of the posterolateral body region, showing the posterior suture. C, frontal view, showing the entrance to the mouth pocket (arrow). Note that somatic kineties enter the mouth pocket to become vestibular kineties. D, optical section through the mouth pocket, showing the vestibular kineties, adoral organelle and paroral membrane. E–G, details of the cell surface, showing the somatic ciliary pattern and the silverline system. Black arrowheads mark the anterior suture, opposed white arrowheads denote the blank stripe within the right lateral suture, red arrows show the dikinetids of the thigmotactic field, and red arrowheads mark the horizontal silverlines. H, detail of the anterior body region, showing the thigmotactic field and the blank stripe within the right lateral suture (opposed white arrowheads). AO, adoral organelle; F, fibres, MP, mouth pockets; PM, paroral membrane; VK, vestibular kineties; SK, somatic kineties. Scale bars = 5 Μm (G), 10 Μm (B), 15 Μm (H), 20 Μm (A, C, E).
Figure 3. M in Re-discovery and novel contributions to morphology and multigene phylogeny of Myxophyllum steenstrupi (Ciliophora: Pleuronematida), an obligate symbiont of terrestrial pulmonates
Figure 3. M. steenstrupi in vivo (A–D) and after protargol impregnation (E, F). A, B, ventral overviews of two representative individuals, showing the nuclear apparatus and the contractile vacuole. Arrow in (A) indicates the entrance to the mouth pocket. C, D, details of the posterior body region, showing the entrance to the mouth pocket (arrow) containing vestibular kineties, the nuclear and contractile vacuole apparatus as well as the posterior suture (opposed white arrowheads). E, top overview, showing the nuclear apparatus and the ciliary pattern. Black arrowheads mark the anterior suture, the white arrowhead denotes the left lateral suture. F, detail of nuclear apparatus of the specimen shown in (E). CV, contractile vacuole; MA, macronuclear nodules; MI, micronucleus; MP, mouth pocket; SK, somatic kineties; TF, thigmotactic field; VK, vestibular kineties. Scale bars = 15 Μm (C), 25 Μm (D), 30 Μm (F), 55 Μm (E), 60 Μm (A, B).
Figure 7 in Re-discovery and novel contributions to morphology and multigene phylogeny of Myxophyllum steenstrupi (Ciliophora: Pleuronematida), an obligate symbiont of terrestrial pulmonates
Figure 7. Phylogenetic tree based on the mitochondrial COI nucleotide sequences, showing the systematic position of M. steenstrupi isolated from the plaited door snail Cochlodina laminata. The class Colpodea was used as the outgroup. Bootstrap values for maximum likelihood conducted in IQTrees as well as posterior probabilities for Bayesian inferences conducted in MrBayes and Phycas were mapped onto the best scoring IQ tree. Dash indicates mismatch between maximum likelihood and Bayesian tree topologies. Sequences marked in red were obtained during this study. Fully statistically supported nodes are marked with red solid circles. GenBank accession numbers can be found in Supporting Information (Table S3). The scale bar denotes twenty substitutions per one hundred nucleotide positions.
FIGURE 2. a in Multigene phylogeny and morphology reveal a new species, Ophiocordyceps vespulae, from Jilin Province, China
FIGURE 2. a. Overview of the stromata and the host. b. Fertile head. c. Longitudinal section showing the complete immersed perithecia. d. Ascomata. e–f. Part asci with apical cap. g–i. Part of ascospores. j, k. Secondary ascospores. l. Upper side of PDA culture. m. Hyphae in PDA culture. Scale bars a = 1 cm, b, c, l = 2 mm, d = 1 mm, e = 100 µm, f, m = 50 µm, g–i = 20 µm, j–k =10 µm.
FIGURE 1 in Multigene phylogeny and morphology reveal a new species, Ophiocordyceps vespulae, from Jilin Province, China
FIGURE 1. Phylogram of Ophiocordyceps vespulae generated from maximum likelihood (RAxML) analysis of combined ITS, LSU, TEF1α and RPB2 sequence data. Tolypocladium inflatum and T. ophioglossoides were the outgroup taxa. Maximum likelihood bootstrap values greater than 75% and posterior probabilities from Bayesian inference ≥ 0.90 are given above the nodes as bootstrap values/Bayesian posterior probabilities. The new species is in red and bold.
Supplementary material 3 from: Katz AM, Barbosa MA, de Oliveira Mattos JL, da Costa WJE (2018) Multigene analysis of the catfish genus Trichomycterus and description of a new South American trichomycterine genus (Siluriformes, Trichomycteridae). Zoosystematics and Evolution 94(2): 557-566. https://doi.org/10.3897/zse.94.29872
Genetics data : Explanation note: Best-fit evolutionary models found for each mitochondrial gene and for each codon position of nuclear genes.
Supplementary material 2 from: Katz AM, Barbosa MA, de Oliveira Mattos JL, da Costa WJE (2018) Multigene analysis of the catfish genus Trichomycterus and description of a new South American trichomycterine genus (Siluriformes, Trichomycteridae). Zoosystematics and Evolution 94(2): 557-566. https://doi.org/10.3897/zse.94.29872
Occurences and morphological data : Explanation note: List of material examined for the analysis of morphological characters.
Supplementary material 1 from: Katz AM, Barbosa MA, de Oliveira Mattos JL, da Costa WJE (2018) Multigene analysis of the catfish genus Trichomycterus and description of a new South American trichomycterine genus (Siluriformes, Trichomycteridae). Zoosystematics and Evolution 94(2): 557-566. https://doi.org/10.3897/zse.94.29872
GenBank accession codes : Explanation note: Terminal taxa for molecular phylogeny and respective GenBank accession numbers.
FIGURE 3 in Morphological and multigene phylogenetic analyses reveal two new nematodetrapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China
FIGURE 3. Arthrobotrys luzhangensis (CGMCC3.20941, holotype!). a Colony. b Microconidia. c Macroconidia. d Chlamydospores. e Trapping-device: adhesive networks. f Microconidiophores. g Macroconidiophores. Bars: a =10 mm; b–g =10 μm.
FIGURE 2 in Morphological and multigene phylogenetic analyses reveal two new nematodetrapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China
FIGURE 2. Arthrobotrys gongshanensis (CGMCC3.23753, holotype!). a Colony on PDA. b, c Conidia. d Chlamydospores. e Trapping device: adhesive networks. f Conidiophores. Bars: a =10 mm; b-f =20 μm.
FIGURE 1 in Morphological and multigene phylogenetic analyses reveal two new nematodetrapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China
FIGURE 1. Maximum likelihood tree based on the combined ITS, EF1-α and RPB2 sequence data. The new isolates are in blue bold, and ex-type strains are in black bold. Bootstrap support values greater than 70% and Bayesian posterior probabilities values greater than 0.90 are indicated above the nodes. The tree is rooted by Vermispora fusarina YXJ02-13-5.
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