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opencc-zeroJul 2023View details →

Impact of multiple soil microbial inoculants on biomass and biomass allocation of the legume crop field pea (Fabaceae: Pisum sativum L.)

Food production is a global challenge and consequently, there is considerable interest in manipulating the rhizobiome using microbial inoculants (MI) to support sustainable agriculture. We investigated how three commercially-available types of plant growth-promoting MI, alone and in combination (B5: five species of Bacillus bacteria, GP: four species of Trichoderma fungi, N2: Paenibacillus polymyxa bacteria) impacted field pea (Fabales: Fabaceae, Pisum sativum L.) in the greenhouse and a two-year field experiment in the United States, North Dakota, NDSU Field Research station at Prosper ND. CON indicates controls that did not receive any MI and FC is the fertilizer control in the field experiment which also did not receive any MI. The dataset consists of data plant data from a 2-wk greenhouse experiment (GH 2wk), a 4-wk greenhouse experiment (GH 4wk), and a two-year field experiment (field). In the greenhouse, we found that effects of MI on plant performance varied, with positive effects of MI only apparent when plants were grown in the winter and likely under greater stress because they lacked nodules. Plants grown in the summer had nodules, and two-week-old MI plants had less root biomass and total plant weight than non-inoculated controls, but weight of four-week-old MI plants was similar to or greater than controls. In the field, the root-to-shoot biomass ratio was highest in non-inoculated controls, and positive effects of N2 on shoots and B5 on shoots and pod densities didn't translate into differences in pod weight or total plant weight. In most cases, plants inoculated with all three inoculants performed similarly to those receiving a single inoculant, while root colonization by arbuscular mycorrhizal fungi (AMF) was higher for B5 plants than plants in the other treatments. This research underscores the need to consider microbial and environmental context when evaluating MI.

opencc-zeroSep 2023View details →

Data for: Assessment of genetic diversity and protein content of Scandinavian peas (Pisum sativum)

We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center by single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (PIC) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over years and accessions. Mean thousand seed weight was 152.05 g. More than 10 accessions had protein content above 28% showing that the collection has potential as a breeding nursery for high-protein pea.

publicSep 2023View details →

Data for: Assessment of genetic diversity and protein content of Scandinavian peas (Pisum sativum)

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publicJul 2023View details →

Impact of multiple soil microbial inoculants on biomass and biomass allocation of the legume crop field pea (Fabaceae: Pisum sativum L.)

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Fig. 8 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 8. Chlorophyll (A) and carotenoid (B) contents (mg/g FW) in pea shoots developed from not primed (NP) and primed (P) seeds at 0 and 120 mM NaCl.The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 10 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 10. Total flavonoid content (mg QE/g FW) in roots and shoots of seedlings developed from not primed (NP) and primed (P) pea seeds at 0 and 120 mM NaCl. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 11 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 11. Total precipitable alkaloids content (mg PAHE/g FW) in roots and shoots of seedlings developed from not primed (NP) and primed (P) pea seeds at 0 and 120 mM NaCl. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 7 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 7. Sodium (a), potassium (b), and phosphor (c) content (in %) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in shoots developed from P and NP seeds, in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 5 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 5. Proline content (μmol/g DW) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in roots and shoots of seedlings developed from P and NP seeds,in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 6 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 6. Total soluble sugars content (mg/g FW) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in roots and shoots of seedlings developed from P and NP seeds, in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 3 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 3. Content of malondialdehyde (MDA) (U/g FW) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in roots and shoots of seedlings developed from P and NP seeds, in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 2 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 2. Electrolyte leakage (%) of (A) pea germinated not primed (NP) and primed (P) seeds and (B) and pea roots and shoots of seedlings developed from P and NP seeds in the presence of NaCl at different concentrations, after an immersion of 24 and 48 h in distilled water. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 1 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 1. Roots and shoots length, developed from primed seeds, at 0 and 120 mM NaCl, expressed in percent of control (seedlings developed from not primed (NP) seeds). Value (N = 4 ± S.E.). Different letters in columns indicate significant differences at p <0.05.

Fig. 9 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 9. Total phenolic content (mg GA/g FW) in roots and shoots of seedlings developed from not primed (NP) and primed (P) pea seeds at 0 and 120 mM NaCl. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

opencc-zeroOct 2020View details →

dryad28/100

Nicotiana benthamiana as a transient expression host to produce auxin analogues: Pisum sativum seed transcriptomic data

Plant secondary metabolites have applications for the food, biofuel, and pharmaceutical industries. Recent advances in pathway elucidation and host expression systems now allow metabolic engineering of plant metabolic pathways to produce "new-to-nature" derivatives with novel biological activities, thereby amplifying the range of industrial uses for plant metabolites. Here we use a transient expression system in the model plant Nicotiana benthamiana to reconstitute the two-step plant-derived biosynthetic pathway for auxin (indole acetic acid) to achieve accumulation up to 500 ng/g fresh mass (FM). By expressing these plant-derived enzymes in combination with either bacterial halogenases and alternative substrates, we can produce both natural and new-to-nature halogenated auxin derivatives up to 990 ng/g FM. Proteins from the auxin synthesis pathway, tryptophan aminotransferases (TARs) and flavin-dependent monooxygenases (YUCs), could be transiently expressed in combination with four separate bacterial halogenases to generate halogenated auxin derivatives. Brominated auxin derivatives could also be observed after infiltration of the transfected N. benthamiana with potassium bromide and the halogenases. Finally, the production of additional auxin derivatives could also be achieved by co-infiltration of TAR and YUC genes with various tryptophan analogues. Given the emerging importance of transient expression in N. benthamiana for industrial scale protein and product expression, this work provides insight into the capacity of N. benthamiana to interface bacterial genes and synthetic substrates to produce novel halogenated metabolites.

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Table 1 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Table 1 Germination percentage (%G) and germination index (GI) of not primed (NP) and primed (P) pea seeds, in presence of 0, 240, and 320 mM NaCl.<table><tbody><tr><th>NaCl (mM)</th><th>Seeds</th><th>% G</th><th>GI</th></tr></tbody><tbody><tr><th>0</th><td>NP</td><td>100a</td><td>5.22b</td></tr><tr><td>P</td><td>100a</td><td>6.72a</td></tr><tr><th>240</th><td>NP</td><td>51.25c</td><td>1.89d</td></tr><tr><td>P</td><td>66.25b</td><td>2.57c</td></tr><tr><th>320</th><td>NP</td><td>0e</td><td>0f</td></tr><tr><td>P</td><td>10d</td><td>0.33e</td></tr></tbody></table>Means with the same letters in a column are not significantly different at p <0.05.