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opencc-by-4.0Nov 2023View details →

Pattern of Using Semaglutide Injection among the Population in Saudi Arabia

Semaglutide injection with lifestyle intervention has been shown to be more effective than lifestyle modification alone. However, pharmacological intervention shows fewer side effects than bariatric surgery. This cross-sectional study was conducted among the general population in Saudi Arabia. A self-administered questionnaire was sent among the targeted population using an online survey. The questionnaire includes socio-demographic data (e.g., age, gender, marital status, etc.), general awareness about Semaglutide injection, and a 4-item questionnaire to measure the knowledge about Semaglutide. Of the 382 participants, 52.4% were males, and 44.8% were aged between 18 to 30  years old. The level of knowledge about Semaglutide injection was poor among 69.4%, while the rest had good knowledge (30.6%). The overall mean knowledge score was 1.62 (SD 1.26) out of 4 points. Younger participants, gender female, unmarried, unemployed, normal or underweight, aware of Semaglutide injection, previous usage of Semaglutide, satisfaction with Semaglutide, and knowledge about combined benefits (diabetes control and weight loss) of Semaglutide injections were the factors associated with increased knowledge score. The knowledge of the general population regarding Semaglutide injection was lacking. Younger female participants with normal body weight who were satisfied with Semaglutide were identified as the most significant users of Semaglutide injections. Further investigations are needed to extract more data about the general population's knowledge of Semaglutide injections.

opencc-by-4.0Nov 2023View details →

The role of injection method on residual trapping at the pore-scale in continuum-scale samples: raw micro-CT dataset

The experiments in this work explore the role of a variable injection rate on gas saturation and residual trapping. There are 2 experiments in this work H2L (high to low injection rate) and L2H (low to high injection rate). The workflow for processing the micro-CT images to get the segmented images is described in [1]. The following scans are included in this repository NB. all data for this repository is segmented micro-CT data.: <ol><li>Dry scan prior to experiment = <a href="https://zenodo.org/api/records/10145102/draft/files/05_dry_bin2_merged.tif/content">05_dry_bin2_merged.tif</a></li><li>Sample fully saturated with brine = <a href="https://zenodo.org/api/records/10145102/draft/files/07_wet_bin2_merged.tif/content">07_wet_bin2_merged.tif</a></li><li>H2L during high flow  = <a href="https://zenodo.org/api/records/10145102/draft/files/09_h2lh_merged.tif/content">09_h2lh_merged.tif</a></li><li>H2L during low flow  = <a href="https://zenodo.org/api/records/10145102/draft/files/11_h2ll_2_merged.tif/content">11_h2ll_2_merged.tif</a></li><li>H2L at the end of drainage (no flow) = <a href="https://zenodo.org/api/records/10145102/draft/files/16_dra1_pd5_merged.tif/content">16_dra1_pd5_merged.tif</a></li><li>H2L at the end of imbibition (no flow) = <a href="https://zenodo.org/api/records/10145102/draft/files/21_imb1_pi1_merged.tif/content">21_imb1_pi1_merged.tif</a></li><li>Sample fully resaturated with brine = <a href="https://zenodo.org/api/records/10145102/draft/files/28_wet2_bin2_merged.tif/content">28_wet2_bin2_merged.tif</a></li><li>L2H during low flow = <a href="https://zenodo.org/api/records/10145102/draft/files/29_2_l2hl_merged.tif/content">29_2_l2hl_merged.tif</a></li><li>L2H during high flow = <a href="https://zenodo.org/api/records/10145102/draft/files/30_l2hh_merged.tif/content">30_l2hh_merged.tif</a></li><li>L2H at the end of drainage (no flow) =<a href="https://zenodo.org/api/records/10145102/draft/files/31_dra2_pd1_merged.tif/content">31_dra2_pd1_merged.tif</a></li><li>L2H at the end of imbibition (no flow)  =<a href="https://zenodo.org/api/records/10145102/draft/files/33_imb2_pi1_merged.tif/content">33_imb2_pi1_merged.tif</a></li></ol>

opencc-by-4.0Dec 2023View details →

Identifying climate impacts from different stratospheric aerosol injection strategies in UKESM1

Data to plot figures in Identifying climate impacts from different stratospheric aerosol injection strategies in UKESM1 - Wells et al., 2023

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbSS livers injected or not with heme

The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease. Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease. All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of livers (indicated foie) from HbSS mice injected or not with heme are presented here.  The results of livers (indicated foie) from HbAA mice injected or not with heme can be found at number 10.5281/zenodo.10963640.  Thirty μm-thick frozen tissue sections of livers were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbAA heart injected or not with heme

The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of heart (indicated coeur) from HbAA mice injected or not with heme are presented here .  The results of heart (indicated coeur) from HbSS mice injected or not with heme can be found at number 10.5281/zenodo.10964159 Thirty μm-thick frozen tissue sections of kidneys were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbSS kidneys injected or not with heme

The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of kidney (indicated rein) from HbSS mice injected or not with heme are presented here .  The results of kidney (indicated rein) from HbAA mice injected or not with heme can be found at number 10.5281/zenodo.10963926. Thirty μm-thick frozen tissue sections of kidneys were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbAA livers injected or not with heme

The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of livers (indicated foie) from HbAA mice injected or not with heme are presented here .  The results of livers (indicated foie) from HbSS mice injected or not with heme can be found at number <a href="https://doi.org/10.5281/zenodo.10962971">10.5281/zenodo.10962971</a>.  Thirty μm-thick frozen tissue sections of livers were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbAA kidneys injected or not with heme

The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of kidney (indicated rein) from HbAA mice injected or not with heme are presented here .  The results of kidney (indicated rein) from HbSS mice injected or not with heme can be found at number  Thirty μm-thick frozen tissue sections of kidneys were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbSS lung injected or not with heme

The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of lung (indicated poumon) from HbSS mice injected or not with heme are presented here .  The results of lung (indicated poumon) from HbAA mice injected or not with heme can be found at number  10.5281/zenodo.10965912 Thirty μm-thick frozen tissue sections of lungs were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbAA lung injected or not with heme

<div> <div> The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of lung (indicated poumon) from HbAA mice injected or not with heme are presented here .  The results of lung (indicated poumon) from HbSS mice injected or not with heme can be found at number 10.5281/zenodo.10966094 Thirty μm-thick frozen tissue sections of lungs were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.  </div> </div>

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice. Dataset for HbSS heart injected or not with heme

<div> The objective of this experiment was to explore the transcriptome of the HbSS Townes mouse model of sickle cell disease.Townes model mice carry several human hemoglobin knock-in genes replacing the endogenous mouse genes and may be useful in studying sickle cell disease.All mice were genotyped, age- and sex-matched littermates. All HbAA (control, normal human hemoglobin) vs HbSS (sickle cell disease, mutated human hemoglobin) mice were used for experimentations at 6-8 weeks of age, to limit intra-group heterogeneity. Hemin (Ferriprotoporphyrin IX) was purchased from Frontiers Scientific and injected intravenously (iv.) in a retroorbital sinus at a concentration of 24 µmol/kg. Control mice received PBS instead. Mice were anesthetized with isoflurane 2-3% for injections, blood collection and sacrifice. All mice were sacrificed by cervical dislocation, 4 hours after injection. The results of heart (indicated coeur) from HbSS mice injected or not with heme are presented here .  The results of heart (indicated coeur) from HbAA mice injected or not with heme can be found at number 10.5281/zenodo.10964042 Thirty μm-thick frozen tissue sections of kidneys were cut as above and homogenized in 200μL of 1-Thioglycerol/Homogenization Solution (Maxwell® 16 LEV simplyRNA Tissue Kit Promega AS1280). The quality and quantity of mRNA were evaluated using a 2100 bioanalyzer with TNA 6000 NanoKits (all Agilent Technologies, Palo Alto, CA, USA). RNA Integrity Numbers superior to 7 were eligible for subsequent reverse transcription into cDNA. RNAseq was performed at the GenomIC plateform Cochin Institute INSERM U1016. After RNA extraction, RNA quality (RNA integrity number) was estimated. 1μg of high-quality total RNA sample (RIN &gt;7) was processed to build up the libraries, using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions. Briefly, purified poly-A containing mRNA molecules were fragmented and reverse-transcribed using random primers. Replacement of dTTP by dUTP during second strand synthesis allowed us to achieve strand specificity. Addition of a single A base to the cDNA was followed by ligation of Illumina adapters. Libraries were quantified by qPCR using KAPA Library Quantification Kits for Illumina Libraries (KapaBiosystems, Wilmington, MA). Library profiles were assessed using DNA High Sensitivity LabChip kits on an Agilent Bioanalyzer. Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode. After sequencing, primary analysis based on AOZAN software (ENS, Paris), was applied to demultiplex and control the quality of the raw data (based of FastQC modules / version 0.11.5). The dataset here represents 4 groups of mice, 4 mice per group as follows: HbAA PBS, HbAA heme, HbSS PBS, HbSS heme.  </div>

opencc-by-4.0Feb 2021View details →

Manipulations pour la micro-injection chez Biomphalaria glabrata

<ol> <li>Production des &oelig;ufs </li> </ol> Mettre dans des aquariums de 5.5L une trentaine d'escargots adultes (10 mm). Mettre un pondoir (morceau de polystyrène de 3 x 3 cm) dans chaque aquarium, c’est le substrat préféré pour faire pondre Biomphalaria glabrata. Les escargots sont nourris ad libitum avec de la salade (des feuilles laitue sans côtes), ils peuvent aussi être nourris avec de la spiruline sèche pour booster la reproduction. Maintenir les aquariums à une température de 25 degrés. <ol> <li>Collection d’&oelig;ufs </li> </ol> Prenez délicatement plusieurs pontes d’&oelig;ufs déposées sur les polystyrènes avec des pinces souples et placez les &oelig;ufs dans une boîte de pétri avec de l’eau minérale naturelle (Volvic de préference) afin d’éviter qu’ils ne sèchent.  Commencez à trier les &oelig;ufs sous la loupe binoculaire pour choisir seulement le stade gastrula et placez-les dans une autre boîte de pétri avec de l’eau minérale naturelle.    <ol> <li>Préparation de la solution de transfection</li> </ol> Matériel : <ol> <li>Réactif de transfection in vivo jetPEI</li> <li>Solution de glucose 10%</li> <li>Solution de glucose 5% </li> <li>Plasmides (dCas9-SunTag-BFP et scFv-DNMT3A-GFP) </li> <li>Microtubes de 0.2 ml</li> <li>Pipettes P10 et P200</li> <li>Pointes de pipettes P10 et P200</li> <li>Marqueur permanent</li> </ol> La solution de glucose et le réactif de transfection in vivo jetPEI sont équilibrés à température ambiante. Préparez 21 µl de chaque plasmide (dCas9-SunTag-BFP et scFv-DNMT3A-GFP) à une concentration de 78.8 et 88.8 ng/µl respectivement (pour un volume total de 42 µl = 3.5 µg d’ADN), ajoutez l’ensemble à un tube de 0.2ml avec 21 µl de solution de glucose à 10% (étiqueté Tube A).    Dans un autre tube de 0.2ml (étiqueté Tube B), 21 µl de solution de glucose à 5% et 1 µl de in vivo jetPEI sont ajoutés. Préparez un autre tube (étiqueté Tube C) avec 21 µl de solution glucose à 5% et 0.5 µl de in vivo jetPEI pour injecter dans des embryons qui serviront de témoins. Laissez les solutions à température ambiante pendant que vous préparez le poste de micro-injection.   <ol> <li>Préparation du poste de micro-injection</li> </ol>   Matériel : <ol> <li>Micropipettes en verre de 1 mm de diamètre étirées</li> <li>Verre de montre</li> <li>Pâte à modeler</li> <li>Boîtes de pétri 35 mm et 90 mm</li> <li>Huile minérale (M5904, SIGMA)</li> <li>Pissette avec de l’eau minérale naturelle (Volvic)</li> <li>Microtubes de 0.2 ml</li> <li>Plaque de culture cellulaire 12 puits</li> <li>Pinceau fin</li> <li>Solution de rouge de phénol</li> <li>Pipette Pasteur</li> <li>Forceps à dissection</li> <li>Pinces souples</li> <li>Pontes d’&oelig;ufs d’escargots au stade gastrula</li> <li>Injecteur de nanolitre programmable Drummond Scientific Nanoject III</li> </ol> Prenez une micropipette préalablement étirée et coupez-la avec un scalpel pour obtenir une pointe d’environ ~0.2 mm légèrement biseautée si possible. Avant de fixer la micropipette à l’injecteur de nanolitre programmable, la remplir d’huile minérale, sans huile minérale à l’intérieur, elle ne fonctionnera pas correctement. Cela peut être réalisé avec une aiguille de remplissage et une seringue hamilton de 10 µl. Lorsque la micropipette est remplie d’huile il faut la fixer sur l’injecteur, pour cela il faut glisser le mandrin et la pince de serrage sur la micropipette en verre étirée, glisser ensuite le joint d’étanchéité le long du piston, puis le positionner. Une fois la micropipette fixée sur l’injecteur, appuyer sur l’icône [EMPTY] jusqu’à ce que le piston soit complètement allongé, cette étape peut être effectuée avec l’interrupteur à pédale en appuyant une fois sur [EMPTY] puis sur [STOP] puis en procédant à vide avec l’interrupteur à pédale. Un seul bip est émis lorsque le piston est complètement allongé.  Remplir la micropipette avec 3 µl de la solution contrôle ou la solution contenant les plasmides (c’est-à-dire la solution de transfection) en plaçant la pointe de la micropipette en verre dans un tube de 0.2 ml avec la solution à injecter et en appuyant sur l’icône [FILL]. Il est souhaitable de la remplir à un débit lent, en appuyant sur l’icône [FILL] pendant quelques secondes, puis sur l’icône [STOP] pour permettre à l’échantillon de s’équilibrer avant d’appuyer de nouveau sur l’icône [FILL].  Remarque : le piston continue de s’allonger ou de se rétracter jusqu’à ce que l’on appuie sur l’icône [STOP], ou jusqu’à ce que la position pleinement allongée ou pleinement rétractée soit atteinte.    <ol> <li>Microinjection</li> </ol> Placez un verre de montre dans une boîte de pétri de 35 mm et fixez le d’un côté avec de la pâte à modeler pour forme une pente. Utilisez une pince souple pour transférer une masse d’&oelig;ufs et posez-la sur le côté de la pente du verre de montre pour que la masse d’&oelig;ufs soit dans une position inclinée.  Enlevez l'excédent d'eau de l'&oelig;uf avec du papier absorbant contre le côté opposé aux &oelig;ufs. Réhydratez si nécessaire avec un pinceau fin pour améliorer la visibilité des embryons. Pour injecter l’échantillon, retourner à l’écran du mode de fonctionnement en appuyant sur l’icône [EXIT], puis sélectionner le mode d’injection en appuyant sur l’icône [INJECT].  Régler le volume d’injection à 30nL et le débit à 20 nL par seconde en utilisant les icônes [+] et [-] respectivement. Appuyez sur l’icône [INJECT] pour injecter l’échantillon. Injectez 30nL de la solution de micro-injection dans chaque &oelig;uf. Placez les masses d’&oelig;ufs micro-injectés dans une plaque de culture cellulaire de 12 puits et notez avec un marqueur s’ils ont été micro-injectés avec la solution de contrôle ou avec la solution contenant les plasmides.  Notez que nous avons coloré avec du rouge phénol la solution d’injection pour faciliter la visibilité dans cette vidéo.  Monitorer l’expression des plasmides  Monitorez l’expression des plasmides 72 h après la micro-injection dans un microscope de contraste/fluorescente ou une loupe binoculaire fluorescente. Puis triez les escargots fluorescents et réalisez une deuxième micro-injection avec une solution contenant 10 µl d’ARN guide (2ng/µl), ainsi que 0.5 µl de réactif in vivo jetPEI et 10 µl de glucose 5%. 3 jours après la deuxième microinjection, récupérez les escargots éclos dans des tubes 1.5 ml contenant 25 µl du tampon de lyse pour une purification d’ADN et ARN. Pour cette photo élaborée au microscope confocal nous avons lavé une larve véligère dans une solution du PBS puis nous l’avons fixé avec une solution du paraformaldéhyde à 4% et ensuite nous l’avons mis dans une lame avec deux gouttes du milieu de montage de fluorescence Dako. 96 heures après la transfection on peut observer l’expression de la protéine verte fluorescente, de la protéine bleue fluorescente et la co-localization des deux protéines.  Ce protocole sert à effectuer des modifications de la méthylation de l’ADN dans un gène cible. Ce protocole de transfection peut être utiliser avec d’autres plasmides, avec des petits ARN interférents ou avec des ARN messagers.  Produit de IHPE (http://ihpe.univ-perp.fr).

opencc-by-4.0Dec 2021View details →

Platicon microcomb generation using laser self-injection locking

This dataset contains data presented in the Figures of the paper "Platicon microcomb generation using laser self-injection locking"

opencc-by-4.0Feb 2022View details →

A Comprehensive Physical Model for the Contrasting Seismogenic Behaviour of Injection Wells in Western Canada

Earthquake catalog for northern Montey play in northeastern British Columbia during 2017-2018.

opencc-by-4.0Apr 2022View details →

ARISE-SAI-1.5: Assessing Responses and Impacts of Solar climate intervention on the Earth system with Stratospheric Aerosol Injection, with cooling to 1.5C

Assessing Responses and Impacts of Solar climate intervention on the Earth system with Stratospheric Aerosol Injection (ARISE-SAI) is a set of simulations carried out with the Community Earth System Model, version 2 with the Whole Atmosphere Community Climate Model, version 6 (CESM2(WACCM6)) that aims at simulating a plausible deployment of solar climate intervention of stratospheric aerosol injection to enable community assessment of responses of the Earth system. This first set of simulations introduce stratospheric aerosol injection at ~ 21 km in simulated year 2035, called ARISE-SAI-1.5, utilize the middle-of-the-road SSP2-4.5 emission scenario,, and keep global mean surface air temperature near 1.5°C above the pre-industrial value. Sulfur dioxide injections in the ARISE-SAI-1.5 simulations are placed at four injection locations (15°S, 15°N, 30°S, 30°N) into one grid box at 180° longitude, and midpoint altitude of 21.6 km. The injection amount at each latitude is specified annually by a “controller” algorithm. This strategy ensures that the global mean surface temperature (T0), north-south temperature gradient (T1), and equator-to-pole temperature gradient (T2) remain close to ~ 1.5°C above the pre-industrial value throughout the simulation.   The files contained here contain output of surface temperature (TREFHT), total precipitation (PRECT), SO4, and controller log files with amounts of SO2 injection.

opencc-by-4.0Apr 2022View details →

Pore matrix dissolution in carbonates: An in-situ experimental investigation of carbonated water injection

Carbonate rocks in underground formations are major targets for oil extraction and carbon storage. The solid part of these porous rocks contains certain minerals, such as calcite and dolomite, that can interact with aqueous solutions. Interactions could be reactive, which leads to the dissolution of these minerals. In this study, we investigated the evolution of carbonate rock dissolution during the flow of carbonated water in pores that initially contain both oil and brine. Carbonated water is an aqueous solution enriched with carbon dioxide (CO2); hence, it is acidic. In our experiments, we observed that the reactive flow and transport of carbonated water is characterized by two distinct periods. The first is a pre-dissolution period where the CO2 molecules diffused from the flowing carbonated water into the oil causing it to swell. As separate oil globules swelled, they reconnected and moved in the direction of the flowing water toward the outlet of the rock sample. In the second stage, significant mineral dissolution occurred creating wormholes that had either a conical or a dominant pattern. The pattern and extent of dissolution was dependent on the flow rate of the carbonated water and its CO2 concentration.

ImPure Injection Molding Sensor Data - Trial 12th May

ImPure project, open access data from PASCOE IM line.

ImPure Injection Molding Sensor Data - Trial 17th May

ImPure project, open access data from PASCOE IM line.

ImPure Injection Molding Sensor Data - Trial 16th May

ImPure project, open access data from PASCOE IM line.

injection of PVA MBs via microcatheter in microfluid channel

jinjection tests operating the syringe manually, also in reverse flux, to verify the absence of obstructions and damages.Thereafter, the injection syringe was mounted on a programmable, step-by-step motor driven syringe pump system which was set at different constant volumetric flow rates, ranging from 0.05 ml/min to 1.12 ml/min according to material and methods, section "Handling of microcatheters with PVA MBs for insertion in microfluidic channels"