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Strong perceptual consequences of low-level visual predictions: a new illusion
<p>Experimental data.</p>
Figure 27. Dorsal habitus, females. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 27. Dorsal habitus, females. A, vertex Pseudoanthidium kaspareki (Between Kula and Usak, Turkey); B, vertex P. rozeni (Hanna, Pakistan); C, mesonotum P. kaspareki (Between Kula and Usak, Turkey); D, mesonotum P. rozeni (Hanna, Pakistan); E, metasoma P. kaspareki (Between Kula and Usak, Turkey); F, metasoma P. rozeni (Hanna, Pakistan).
Figure 23. Gonostyli. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 23. Gonostyli. A, Pseudoanthidium palestinicum (Nahal Keziv, Israel); B, P. cribratum (Karatau, Kazakhstan); C, P. canariense (Santa Cruz de Tenerife, Canary Islands); D, P. tropicum (Bandar Abbas, Iran); E, P. kaspareki (Side, Turkey); F, P. rozeni (Hanna, Pakistan).
Figure 22 in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 22. Distribution of A, Pseudoanthidium palestinicum; B, P. cribratum; C, P. canariense; D, P. tropicum.
Figure 19. Lectotype Pseudoanthidium tenellum. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 19. Lectotype Pseudoanthidium tenellum. A, dorsal view; B, lateral view; C, T1–T3; D, labels.
Figure 17. Dorsal habitus, females. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 17. Dorsal habitus, females. A, vertex Pseudoanthidium stigmaticorne (Arzens, France); B, vertex P. cribratum (Bukhara, Uzbekistan); C, mesonotum P. stigmaticorne (Arzens, France); D, mesonotum P. cribratum (Bukhara, Uzbekistan); E, metasoma P. stigmaticorne (Arzens, France); F, metasoma P. cribratum (Bukhara, Uzbekistan).
Figure 13. S2, males. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 13. S2, males. A, Pseudoanthidium nanum (Dordogne, France); B, P. scapulare (Argèles-sur-Mer, France); C, P. stigmaticorne (Cabanes de Fleury, France); D, P. tenellum (Krasnoperekopsk, Crimea); E, P. palestinicum (Jerusalem, Israel); F, P. cribratum (Ayelet Hashar, Israel); G, P. kaspareki (Side, Turkey); H, P. rozeni (Hanna, Pakistan).
Figure 11. Sternal combs. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 11. Sternal combs. A, Pseudoanthidium nanum (Dordogne, France); B, P. scapulare (Argèles-sur-Mer, France); C, P. stigmaticorne (Cabanes de Fleury, France); D, P. tenellum (Krasnoperekopsk, Crimea); E, P. palestinicum (Jerusalem, Israel); F, P. cribratum (Ayelet Hashar, Israel); G, P. kaspareki (Side, Turkey); H, P. rozeni (Hanna, Pakistan).
Figure 12. S3, males. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 12. S3, males. A, Pseudoanthidium nanum (Dordogne, France); B, P. scapulare (Argèles-sur-Mer, France); C, P. stigmaticorne (Cabanes de Fleury, France); D, P. tenellum (Krasnoperekopsk, Crimea); E, P. palestinicum (Jerusalem, Israel); F, P. cribratum (Ayelet Hashar, Israel); G, P. kaspareki (Side, Turkey); H, P. rozeni (Hanna, Pakistan).
Figure 10. Gonostyli. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 10. Gonostyli. A, Pseudoanthidium nanum (Thüringen, Germany); B, P. scapulare (Villeneuve-lès-Maguelone, France); C, P. tenellum (Krasnoperekopsk, Crimea).
Figure 8. Lectotype Icteranthidium floripetum A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 8. Lectotype Icteranthidium floripetum A, face; B, dorsal view; C, labels; D, vertex; E, T1–T3.
Figure 18. Gonostyli, Pseudoanthidium stigmaticorne. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 18. Gonostyli, Pseudoanthidium stigmaticorne. A, Cape Lukull, Crimea; B, Bet She'an, Israel; C, Agadir, Morocco; D, Restinclières, France; E, Cacela Velha, Portugal; F, Seui, Sardinia.
Figure 25. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 25. A, Pseudoanthidium canariense, female (Santa Cruz de Tenerife, Canary Islands); B, P. tropicum, female (Bandar Abbas, Iran); C, P. canariense, male (Santa Cruz de Tenerife, Canary Islands); D, P. tropicum, male (Bandar Abbas, Iran); E, T7, P. canariense (Santa Cruz de Tenerife, Canary Islands); F, T7, P. tropicum (Bandar Abbas, Iran).
Figure 7. Lectotype, Anthidium sinuatum. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 7. Lectotype, Anthidium sinuatum. A, lateral view; B, labels; C, metasoma; D, T5–T6; E, mesonotum.
Figure 21. Dorsal habitus, females.A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 21. Dorsal habitus, females.A, vertex Pseudoanthidium tenellum (Pestszentimre, Hungary); B, vertex P. palestinicum (Rehovot, Israel); C, mesonotum P. tenellum (Pestszentimre, Hungary); D, mesonotum P. palestinicum (Rehovot, Israel); E, metasoma P. tenellum (Pestszentimre, Hungary); F, metasoma P. palestinicum (Rehovot, Israel).
Figure 6 in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 6. Palaearctic distribution of A, Pseudoanthidium nanum; B, P. scapulare; C, P. stigmaticorne; D, P. tenellum.
Figure 24 in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 24. Third coxal tooth (circled by white ring), Pseudoanthidium cribratum, male. A, ventral view (Damascus, Syria); B, lateral view (Yasuj, Iran).
Figure 16. Lectotype Pseudoanthidium karakalense. A in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 16. Lectotype Pseudoanthidium karakalense. A, labels; B, dorsal view; C, latero-ventral view S2; D, gonostyli; E, S8; F, S3; G, S4; H, S5 (showing sternal combs), S6.
Figure 2 in Paraphyly and low levels of genetic divergence in morphologically distinct taxa: revision of the Pseudoanthidium scapulare complex of carder bees (Apoidea: Megachilidae: Anthidiini)
Figure 2. Best-scoring maximum likelihood tree based on analysis of COI data. Numbers shown at nodes are maximum likelihood bootstrap values based on 1000 bootstrap replicates in RAxML. Only bootstrap values greater than 50% are shown. Terminals are labelled with a DNA extraction code, species name, collection locality and either as male (m) or female (f). Barcodes were obtained using Sanger sequencing technology, except for those corresponding to specimens 1802 and 1805, which were obtained from non-UCE assemblies generated during UCE sequencing. A, Pseudoanthidium tenellum, Burgenland, Austria (m), photo Bernhard Jacobi; B, P. cribratum, Bukhara, Uzbekistan (f), photo Jessica Litman; C, P. canariense, Santa Cruz de Tenerife, Canary Islands, Spain (m), photo Jessica Litman; D, P. stigmaticorne, Crimea, Russia, photo Alexander V. Fateryga; E, P. scapulare, Portugal, photo Ian Cross; F, P. nanum, photo Entomologie/Botanik, ETH Zürich / Albert Krebs; G, P. palestinicum, photo Jessica Litman.
Raw single-molecule imaging data for "Tuning levels of low-complexity domain interactions to modulate endogenous oncogenic transcription"
<p><strong>Raw single-molecule imaging data for "Tuning levels of low-complexity domain interactions to modulate endogenous oncogenic transcription"</strong></p> <p>Shasha Chong<sup>1</sup>, Thomas G.W. Graham<sup>2</sup>, Claire Dugast-Darzacq<sup>2,5</sup>, Gina M. Dailey<sup>2</sup>, Xavier Darzacq<sup>2,5</sup>, Robert Tjian<sup>2,3,4,5</sup>*</p> <p><sup>1 </sup>Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA</p> <p><sup>2 </sup>Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.</p> <p><sup>3 </sup>Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.</p> <p><sup>4</sup><sup> </sup>Li Ka Shing Center for Biomedical & Health Sciences, University of California, Berkeley, CA, USA.</p> <p><sup>5</sup><sup> </sup>CIRM Center of Excellence, University of California, Berkeley, CA. </p> <p>* Lead contact</p> <p><strong>Overview</strong></p> <p>This repository contains 1) movies of endogenously expressed EWS::FLI1-Halo in genome-edited A673 cells acquired using stroboscopic photo-activatable single particle tracking (spaSPT) and 2) images of exogenously expressed mNeonGreen-EWS-NPM1 fusion protein in the above cells before and after spaSPT movies were acquired. The uploaded files include data acquired from 80 live cells on 4 different days. The imaging data, after being processed, were used to generate Figure 4C-E of the manuscript in the title. </p> <p><strong>Method details</strong></p> <p>The genome-edited A673 cells (described in https://www.science.org/doi/10.1126/science.aar2555) with inducible expression of mNeonGreen-EWS-NPM1 were grown on 25 mm circular No. 1.5 cover glasses (Azer Scientific, 200251) that were plasma-cleaned prior to use. We induced the cells with 200 ng/ml of doxycycline for 96 hours, stained the cells with 20 nM PA-JF646 and 200 nM JFX549 HaloTag ligands, and performed single-molecule imaging of EWS::FLI1-Halo on a custom-built Nikon (Nikon Instruments Inc.) TI microscope described in (https://elifesciences.org/articles/25776). We took images with a 100x/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100x Oil) under highly inclined and laminated optical sheet (HILO) illumination (https://www.nature.com/articles/nmeth1171) using following laser lines: 488 nm for mNG; 561 nm for JFX549; 405 nm and 633 nm for photo-activation and excitation of PA-JF646, respectively. The incubation chamber maintained a humidified 37°C atmosphere with 5% CO<sub>2</sub> and the objective was similarly heated to 37°C for live-cell experiments. </p> <p>High-concentration JFX549 staining allows visualization of the intracellular distribution of EWS::FLI1-Halo. We chose cells with EWS::FLI1-Halo enriched in the nucleolus to perform spaSPT. The procedure of spaSPT largely follows what is described in (https://elifesciences.org/articles/25776). Both the excitation laser (633 nm) and the photo-activation laser (405 nm) for PA-JF646 were pulsed. Each frame consisted of a 7-ms camera exposure time followed by a ~500 μs camera ‘dead’ time. The excitation laser (633 nm) was pulsed for 1 ms starting at the beginning for the 7 ms camera exposure time. The photo-activation laser (405 nm) was pulsed during the ~500 μs camera ‘dead’ time, minimizing fluorescence background. Each cell was imaged for 20,000 frames corresponding to ~1.5 min. Images of mNeonGreen-EWS-NPM1 were collected with a camera exposure time of 500 ms before and after the acquisition of each spaSPT movie.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.