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190 results for “molecular barcoding”
Figure 1 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species
Figure 1. Atlantoscia inflata sp. nov., male holotype MZUSP 32622. A, habitus, dorsal view; male paratype MZUSP 32623; B, scale-seta; C, cephalothorax, frontal view; D, cephalothorax, dorsal view; E, noduli laterales coordinates d/c; F, noduli laterales coordinates b/c; G, pleotelson; H, antennula; I, antenna; J, left mandible; K, right mandible; L, maxillula, outer endite; M, maxilla; N, maxilliped.
Figure 5. Consensus tree obtained from a 640 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species
Figure 5. Consensus tree obtained from a 640-bp alignment of cytochrome c oxidase subunit I gene sequences of the Atlantoscia species by using Bayesian inference. Numbers at nodes represent posterior probabilities values (1 000 000 generations). Clades highlighted by grey shading in the tree correspond to nominal species of Atlantoscia. Letters (a–k) represent the different individuals of terrestrial isopods analysed.
Figure 2 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species
Figure 2. Atlantoscia inflata sp. nov., male paratype MZUSP 32623. A, pereopod 1; B, pereopod 7; C, uropod; D, genital papilla; E, pleopod 1; F, pleopod 2; G, pleopod 3 exopod; H, pleopod 4 exopod; I, pleopod 5 exopod.
Fig. 9 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 9. Light micrographs of cuticles from paratypes and hologenophores mounted after DNA extraction and COI sequencing. (A–B) Echinoderes horni, NHMD- 1176472, GenBank Acc. No. OP617666. (C–D) Echinoderes wilberti sp. nov., NHMD- 1176460, GenBank Acc. No. OP617672. (A) Dorsal overview. (B) Segments 1 to 6, dorsal view. (C) Dorsal overview. (D) Segments 1 to 6, dorsal view. Arrows indicate subdorsal tubes on segment 2.
Fig. 3 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 3. Diagram of mouth cone (grey area), introvert and placids in Echinoderes abeli sp. nov., outer oral styles (diamonds), primary scalids (triangles), spinoscalids (open circles), and trichoscalids (stars), with positions of trichoscalid plates and placids indicated. Table shows the scalid arrangement by sector; single-lined boxes mark quincunxes, double-lined boxes mark "double diamonds", question mark indicates unknown information.
Fig. 1 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 1. Maps showing (A) the position of areas of study in Mexico (B) sampling stations at Islas Marias with asterisks marking St. 8 y St.12, and (C) sampling stations in Quintana Roo with inset showing zoom of stations in Xcalak.
Fig. 6 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 6. Line art illustrations of Echinoderes wilberti sp. nov. (A) Female, dorsal view. (B) Female, ventral view. (C) Segments 10 to 11 in male, dorsal view. (D) Segments 10 to 11 in male, ventral view. Abbreviations: lat, lateral accessory tube; ldss, laterodorsal sensory spot; ldt, laterodorsal tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvgco1, lateroventral glandular cell outlet type 1; lvs, lateroventral spine; lvt, lateroventral tube; mdgco1, middorsal glandular cell outlet type 1; mlss, midlateral sensory spot; pdgco1, paradorsal glandular cell outlet type 1; pe, penile spines; pvb, paraventral bristles; sdss, subdorsal sensory spot; sdt, subdorsal tube; vlss, ventrolateral sensory spot; vmgco1, ventromedial glandular cell outlet type 1; vmss, ventromedial sensory spot.
Fig. 5 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 5. Scanning electron micrographs showing overviews and details of Echinoderes abeli sp. nov. (A) Dorsal overview. (B) Ventral overview. (C) Mouth cone, dorsal view. (D) Introvert sector 2 (paraventral). (E) Segments 1 to 2, dorsal view. (F) Segment 1 to 2, ventral view. (G) Segments 7 to 8, lateral view. (H) Segments 2 to 4, lateral view; inset shows close-up of glandular cell outlet type 2. (I) Segments 10 to 11, dorsal view, showing female sexual dimorphism. (J) Segments 10 to 11, ventral view, showing female sexual dimorphism. (K) Segments 10 to 11, laterodorsal view, showing female sexual dimorphism; inset shows close-up of laterodorsal tube. Abbreviations: ldss, laterodorsal sensory spot; ldt, laterodorsal tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvt, lateroventral tube; mdss, middorsal sensory spot; mlt, midlateral tube; oos, outer oral styles; pdss, paradorsal sensory spot; pr, protuberance; psp, primary spinoscalid; sdgco2, subdorsal glandular cell outlet type 2; sdss, subdorsal ss; slt, sublateral tube; sp, spinoscalid followed by introvert ring number; tr, trichoscalid; vlss, ventrolateral sensory spot; vmss, ventromedial sensory spot. Digit after abbreviation in lvt refer to segment number.
Fig. 10 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 10. Line art illustrations of Echinoderes horni Higgins, 1983. (A) Female, dorsal view. (B) Female, ventral view. (C) Segments 10 to 11 in male, dorsal view. (D) Segments 10 to 11 in male, ventral view. Abbreviations: lat, lateral accessory tube; ldss, laterodorsal sensory spot; ldt, laterodorsal tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvgco1, lateroventral glandular cell outlet type 1; lvs, lateroventral spine; lvt, lateroventral tube; mdgco1, middorsal glandular cell outlet type 1; mlss, midlateral sensory spot; pdgco1, paradorsal glandular cell outlet type 1; pe, penile spines; pvb, paraventral bristles; sdss, subdorsal sensory spot; vlss, ventrolateral sensory spot; vmgco1, ventromedial glandular cell outlet type 1; vmss, ventromedial sensory spot.
Fig. 12 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies
Fig. 12. Confocal laser micrographs of Echinoderes horni (NHMD-1176472) showing focal overview sections, from most dorsal in (A) and a deeper focus through (B) to (C). Sensory spots are indicated by full circles, and glandular cell outlets type 1 by dashed circles.
Figure 1 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 1. Phylogenetic tree based on the 18S rRNA gene, ITS1-5.8S-ITS2 region and 28S rRNA gene, showing relationships among ciliates isolated from the digestive tract of panesthiine cockroaches. All tree-building methods resulted in very similar topologies. The single exception is the Anteclevelandella constricta cluster, where the IQTree topology differs from that of both Bayesian trees (shown in the box). Bootstrap values for the maximum likelihood conducted in IQTrees and posterior probabilities for Bayesian inferences conducted in MrBayes and Phycas are listed at corresponding nodes of the best scoring IQTree. Specimen codes and further details are listed in Table 1. The scale bar denotes two substitutions per one hundred nucleotide positions.
Figure 4 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 4. Consensus secondary structure of the ITS2 molecule of 54 members of the order Clevelandellida isolated from the digestive tract of cockroaches. Note that the central loop radiates four highly conserved helices. The structure logo of helices is shown on the right side. The height of a base is proportional to its frequency in the multiple sequence alignment.
Figure 3 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 3. MDS diagrams (A, C, E) and TCS networks (B, D, F) based on 18S rRNA gene (A, B), ITS1-5.8S-ITS2 region (C, D) and 28S rRNA gene (E, F) sequences of the family Clevelandellidae. The MDS diagrams show the distribution of specimens in the genotype space. Species belonging to the same genus form a cluster distinctly isolated from other such clusters. The TCS networks reflect the most parsimonious relationships among species given the individual markers. Numbers along edges indicate mutational steps between adjacent nodes. Species belonging to the same genus are marked by the same colour code.
Figure 7 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 7. Putative secondary structure models of the highly divergent helix c3-1 in the D2 domain of the 28S rRNA molecule of 17 species of the order Clevelandellida isolated from the digestive tract of cockroaches. Arrowheads denote the molecular diagnostic characters.
Figure 2. Coalescent species trees with 13 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 2. Coalescent species trees with 13 (A) and 14 (B) assumed species based on 18S rRNA gene, ITS1-5.8S-ITS2 region and 28S rRNA gene sequences. Posterior probabilities of clades are provided along internal branches and posterior probabilities for the presence of individual species are provided behind the terminal branches. Scale bars denote the fraction of substitutions per site.
Figure 9 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 9. MDS diagram (A) and TCS network (B) based on concatenated 18S rRNA gene, ITS1-5.8S-ITS2 region and 28S rRNA gene sequences of the family Clevelandellidae. The MDS diagram shows the distribution of specimens in the genotype space. Species belonging to the same genus form a cluster distinctly isolated from other such clusters. The TCS network reflects the most parsimonious relationships among species given the concatenated dataset. Numbers along edges indicate mutational steps between adjacent nodes. Species belonging to the same genus are marked by the same colour code.
Figure 6 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 6. Putative secondary structure models of helices 39‒44 in the V7 region of the 18S rRNA molecule of four Nyctotherus species isolated from the digestive tract of cockroaches. Arrowheads denote the molecular diagnostic characters. CBC, compensatory base change; hemi-CBC, hemi-compensatory base change.
Figure 5 in DNA barcoding and coalescent-based delimitation of endosymbiotic clevelandellid ciliates (Ciliophora: Clevelandellida): a shift to molecular taxonomy in the inventory of ciliate diversity in panesthiine cockroaches
Figure 5. Putative secondary structure models of the highly divergent helix 23e1 in the V4 region of the 18S rRNA molecule of 17 species of the order Clevelandellida isolated from the digestive tract of cockroaches. Arrowheads denote the molecular diagnostic characters.
FIGURE 19 in Morphology, molecular phylogenetics, and DNA barcoding revealed a new unusual species of the aphid genus Pleotrichophorus from the USA (Insecta, Hemiptera Aphididae)
FIGURE 19. Maximum-likelihood bootstrap consensus tree of Macrosiphini taxa. Pleotrichophorus samples new to this study are highlighted by the peach-colored box. Capitophorus species are highlighted by the blue box. The misidentified specimen of Pleotrichophorus glandulosus specimen reported by Choi et al. (2018) was recovered in a clade along with members of the genus Capitophorus. Support values from left to right are ML ultrafast bootstrap (>95; blue dot), standard non-parametric ML bootstrap (>75; red dot), and MP ultrafast bootstrap (>95; green dot). Nodes without labeled support values indicate that the support value was not significant for that analysis or was not recovered by that analysis.
FIGURE 18. K2P in Morphology, molecular phylogenetics, and DNA barcoding revealed a new unusual species of the aphid genus Pleotrichophorus from the USA (Insecta, Hemiptera Aphididae)
FIGURE 18. K2P neighbor-joining tree of Capitophorus and Pleotrichophorus COI barcodes with auto-collapsed clades with 0.005> average branch length. Collapsed terminal clusters are indicated by red, green, blue, or orange circles. Bootstrap values higher than 80% are indicated on branches with gray circles. Capitophorus species are indicated by blue branches. Pleotrichophorus glandulosus sensu Choi et al. 2018 and Capitophorus sp. from South Korea, China, and California are indicated in orange. Pleotrichophorus species are indicated by red branches. Pleotrichophorus blackmani sp. n., is indicated in green. COI barcodes identified as Pleotrichophorus glandulosus, P. pseudoglandulosus, and P. sp. from France, Russian Far East, and Eastern Canada are indicated in purple.
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