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Fig. 6 in Description of two new species forming a sympatric species pair of Crenicichla (Teleostei: Cichlidae) endemic to the Piray Guazú River in the Paraná River Basin, Misiones, Argentina and belonging to the C. mandelburgeri species complex

Fig. 6. Type locality of C. ama sp. nov. and C. aravera sp. nov., upper arroyo Piray Guazú on RP20 (26°26′34.1″ S, 54°08′29.4″ W), 18 February 2012.

Fig. 5. A in Description of two new species forming a sympatric species pair of Crenicichla (Teleostei: Cichlidae) endemic to the Piray Guazú River in the Paraná River Basin, Misiones, Argentina and belonging to the C. mandelburgeri species complex

Fig. 5. A.Topographic map of the Middle Paraná Basin. B. Satellite map of the Piray Guazú Basin showing distribution of the sympatric C. ama sp. nov. (dark green) and C. aravera sp. nov. (light green), and also of other species in the Iguazú (red) and the Middle Paraná (C. ypo Casciotta, Almirón, Piálek, Gómez & Říčan, 2010: dark orange; C. yjhui: orange; C. hu: yellow; C. mandelburgeri sensu lato: blue, C. gillmorlisi: grey; C. taikyra: pink). Large and small dots in the Piray Guazú Basin show localities where the species is common or rare, respectively. Dark blue and light blue dots show the northern and southern mtDNA clades of C. mandelburgeri, respectively. White bars show location of significant waterfalls. White dot (marked with X in B) shows an upstream locality in the Piray Guazú where both C. ama and C. aravera are absent. Locality 1 (in B, marked with a star) is the type locality of both new species. Localities 2 and 3 (in B) are paratype localities of the two new species. Inset in A shows the parallel species pairs in the Piray Guazú and Urugua-í basins.

Fig. 2 in Description of two new species forming a sympatric species pair of Crenicichla (Teleostei: Cichlidae) endemic to the Piray Guazú River in the Paraná River Basin, Misiones, Argentina and belonging to the C. mandelburgeri species complex

Fig. 2. Crenicichla ama sp. nov., live specimens. A–B. Male paratypes. C–E. Female paratypes. A. MLP 11448, tributary to upper arroyo Piray Guazú on RP16 23 km from San Pedro, 26°35′45.90″ S, 54°16′59.96″ W, November 26 2016. B. MLP 11182, 94.5 mm. C–D. MLP 11180. E. MLP 11448, tributary to upper arroyo Piray Guazú on RP16 23 km from San Pedro, 26°35′45.90″ S, 54°16′59.96″ W, November 26 2016).

Fig. 3 in Description of two new species forming a sympatric species pair of Crenicichla (Teleostei: Cichlidae) endemic to the Piray Guazú River in the Paraná River Basin, Misiones, Argentina and belonging to the C. mandelburgeri species complex

Fig. 3. Crenicichla aravera sp. nov., live specimens. A. Holotype, ♂ (MLP 11450). B. Paratype, ♂ subadult (MLP 11451, 81.2 mm). C–D. Female paratypes (MLP 11451, 83.3 mm; MLP 11452, 93.7 mm), upper arroyo Piray Guazú on RP20, February 18 2012.

Fig. 1. A in Description of two new species forming a sympatric species pair of Crenicichla (Teleostei: Cichlidae) endemic to the Piray Guazú River in the Paraná River Basin, Misiones, Argentina and belonging to the C. mandelburgeri species complex

Fig. 1. A. Crenicichla ama sp. nov., holotype, ♂ (MLP 11446, 105.8 mm). B. Crenicichla aravera sp. nov., holotype, ♂ (MLP 11450, 102.5 mm).

Fig. 7 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 7. Box plot of the morphometric traits that differed statistically (Mann-Whitney, p <0.0039) between allopatric (Allo) and sympatric (Sym) populations of A – Tettigettalna argentata and B – Tettigettalna mariae. All measurements are in millimetres. See Table 2 for description of the morphometric traits.

Fig. 6 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 6. Scatter plot of the 69 Tettigettalna argentata and Tettigettalna mariae occurring allopatrically (Allo) and sympatrically (Sym) in the first two PCA components extracted from a correlation matrix composed of 13 acoustic variables. See ST3 for details of the Eigen analysis and factor loadings.

Fig. 4 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 4. Scatter plot of the 176 specimens of Tettigettalna in the two PCA components extracted from a correlation matrix composed of 22 morphometric traits. See ST2 for details of the Eigen analysis and factor loadings.

Fig. 2 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 2. The traits of external morphology of specimens of Tettigettalna measured. A – dorsal view; B – ventral view of the head; C – anterior femur; D – operculum; E – tymbal; F – anterior wing; G – posterior wing. (See Table 2 for detailed description).

Fig. 8 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 8. Scatter plot of the 50 Tettigettalna argentata and Tettigettalna mariae occurring allopatrically (Allo) and sympatrically (Sym) in the first two PCA components extracted from a correlation matrix composed of measurements of 22 morphometric traits. See ST4 for details of the Eigen analysis and factor loadings.

Fig. 5 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 5. Box plot of the acoustic variables that differed statistically (Mann-Whitney, p <0.0039) between allopatric (Allo) and sympatric (Sym) populations of A – Tettigettalna argentata and B – Tettigettalna mariae. Time is in seconds and frequency in kHz.

Fig. 3 in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 3. Scatter plot of the 193 specimens of Tettigettalna in the first two PCA components extracted from a correlation matrix composed of 13 acoustic variables. See ST1 in Supplementary Tables for details of the Eigen analysis and factor loadings.

Fig. 1. A in Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): Sympatric populations show unexpected differences

Fig. 1. A – Map of the south-western part of the Iberian Peninsula showing the locations of the allopatric and sympatric populations of the four species of Tettigettalna sampled. B – More detailed map of the area marked by the rectangle in A. Letters: allopatric populations of: a – T. argentata; e – T. estrellae; j – T. josei and m – T. mariae. Symbols: Sympatric populations of: circle – T. argentata and T. estrellae; square – T. argentata and T. josei; triangle: T. argentata and T. mariae and cross: T. argentata, T. mariae and T. josei. Map produced by the program DIVA_GIS (LizardTechSeattle, USA) using the spatial data provided by this program.