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190 results for “targeted enrichment”
FIGURE 37 Modica confusa comb.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 37 Modica confusa comb.n. (a) male wing venation; (b and c) male (FLMNH-MGCL-269550) dorsal (b) and ventral (c); (d and e) female dorsal (d) and ventral (e). Scale bars 1 cm.
FIGURE 26 Trico tricolor tricolorcomb.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 26 Trico tricolor tricolorcomb.n. (a) male wing venation; (b and c) male dorsal (b) and ventral (c); (d and e) female dorsal (d) and ventral (e). Scale bars 1 cm.
FIGURE 13 Lazulina gen.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 13 Lazulina gen.n. Species diversity mapped on a 2 degree grid. Colours ranging from dark green to red represent increasing diversity.
FIGURE 11 Lazulina hewitsoniicomb.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 11 Lazulina hewitsoniicomb.n. (a) male wing venation; (b) male forewing androconial scales posterior of cubital vein; (c and d) male dorsal (c) and ventral (d); (e and f) female dorsal (e) and ventral (f); (g) male abdomen lateral view. Scale bars 1 cm unless otherwise noted.
FIGURE 12 Lazulina hewitsonii comb.n. A-D in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 12 Lazulina hewitsonii comb.n. A-D, male genitalia (dissection KW-15-21), lateral (a), dorsal (b), aedeagus lateral (c) and aedeagus dorsal (d); (e and f) female genitalia (dissection SN-17-207), ventral view exterior tip abdomen (e), dorsal view interior abdomen (f). Scale bars 1 mm.
FIGURE 10 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 10 Phylogeny of the 'Splendeuptychia clade' based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 9 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 9 Phylogeny of the 'Pareuptychia clade' based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 6 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 6 Phylogeny of the Amphidecta clade based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 4 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 4 Phylogeny of the Megisto clade based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 8 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 8 Phylogeny of the 'Archeuptychia clade' + Chloreuptychia based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfy images equal 1 cm.
FIGURE 5 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 5 Phylogeny of the Hermeuptychia clade and nearby genera based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 7 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 7 Phylogeny of the 'Taygetis clade' based on the FULL dataset. Interpretation of the support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 3 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 3 Phylogeny of the Cyllopsis clade based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
Assessment of targeted enrichment locus capture across time and museums using odonate specimens
<p><span>The use of gDNA isolated from museum specimens for high throughput sequencing, especially targeted sequencing in the context of phylogenetics, is a common practice. Yet, little understanding has been focused on comparing the quality of DNA and the results of sequencing museum DNAs. Dragonflies and damselflies are ubiquitous in freshwater ecosystems and are commonly collected and preserved insects in museum collections hence their use in this study. However, the history of odonate preservation across time and museums have resulted in wide variability in the success of viable DNA extraction, necessitating an assessment of their usefulness in genetic studies. Using Anchored Hybrid Enrichment probes, we sequenced DNA from samples at two museums, 48 from the American Museum of Natural History (AMNH) in NYC, USA, and 46 from the Naturalis Biodiversity Center (RMNH) in Leiden, Netherlands ranging from global collection localities and across a 120-year time span. We recovered at least 4 loci out of an >1000 locus probe set for all samples, with the average capture being ~385 loci. Neither specimen age nor size was a good predictor of locus capture, but recapture rates differed significantly between museums. Samples from the AMNH had lower overall locus capture than the RMNH, perhaps due to differences in specimen storage over time.</span></p>
FIGURE 2 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 2 Phylogeny of Euptychia based on the FULL dataset. Interpretation of support values can be found in the legend. The scale bars under the butterfly images equal 1 cm.
FIGURE 1 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)
FIGURE 1 Summary tree based on the FULL dataset showing the major clades supported in this study. Colours are the same as in Espeland et al. (2019a). Support for major clades is shown. Interpretation of support values can be found in the legend.
Data from: Universal target-enrichment baits for anthozoan (Cnidaria) phylogenomics: new approaches to long-standing problems
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Data from: Target enrichment of ultraconserved elements from arthropods provides a genomic perspective on relationships among Hymenoptera
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Data from: A protocol for targeted enrichment of intron-containing sequence markers for recent radiations: a phylogenomic example from Heuchera (Saxifragaceae)
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Analysis of RNA-seq, DNA target enrichment, and Sanger nucleotide sequence data resolves deep splits in the phylogeny of cuckoo wasps (Hymenoptera: Chrysididae)
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