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19,342 results for “Sequences”
Raw Fast5 data for "Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon" - PART I
<p>Raw Fast5 data for "Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon". See Supplementary Table 2 for associating each sample to its barcode.</p> <p>- FC1_1 includes data for the HM mock community from BEI resources and skin microbiome of the chin in dogs.</p> <p>- FC1_2 includes data for the dorsal skin samples</p> <p>- FC2 includes data for the Zymobiomics mock community and Staphylococcus pseudintermedius isolate</p> <p> </p> <p> </p>
Branching Trees from standard Epidemic Aftershock Sequences (ETAS) Model (no time, no space)
<p>All files licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)</p> <p>###############<br> 0. SUMMARY<br> ###############</p> <p>1. DESCRIPTION</p> <p>2. INPUT PARAMETERS</p> <p>3. TYPES OF FILES<br> 3.1. Raw data<br> 3.2. List of trees<br> 3.3. Tree-size frequencies</p> <p>4. LIST OF FILES<br> 4.1. Raw data<br> 4.2. List of trees<br> 4.3. Tree-size frequencies<br> 4.4 Known missing/broken files</p> <p>###############<br> 1. DESCRIPTION<br> ################<br> Simulation results of an standard ETAS model as a branching process. Using a two seed version of the RANDU linear congruential pseudorandom number generator. The offspring number is a Poisson number given the rate n(M) (see below). Details of simulation procedure can be found in reference [1]: 'Topological properties of epidemic aftershock processes', by J. Baró submitted to J. of Geophysical Research - Solid Earth (JGR-B)</p> <p>##############<br> 2. INPUT PARAMETERS<br> ##############<br> The input parameters (see reference for details) for each raw and processes data-file are indicated in the prefix of the file: "ETASbranch_b(b)r(r)N(nb)*"</p> <p>- M0(= 1) = magnitude of completeness (arbitrary for the study of topological properties of trees)<br> - (b) = b-value (arbitrary for the study of topological properties of trees)<br> - (nb) = average branching ratio<br> - (r) = ratio a/b</p> <p>The b-value defines the distribution of event-magnitudes: P(M) = 10^(b*(M-M0)) . The nb and a define the productivity law: n(M) = (nb*(b-a)/b)*10^(a*(M-M0))</p> <p><br> #################<br> 3. TYPES OF FILES<br> #################</p> <p>3.1. Raw data:<br> --------------</p> <p>42 x "*.Seq" files with input b=0.50 and different nb, r values. Raw data from simulation code. (all cases, simulated with 10^5 background events)<br> Each row represents an individual event in the point process, or element of the simulated branching forest.<br> Columns description (9 columns x data point):<br> c0:Time (arbitrary, used here as id.)<br> c1:Magnitude of the event<br> c2:Identification number of the cluster or tree<br> c3:Depth of the event in the tree structure (background events have Depth = 0)<br> c4:Time of the direct parent of the event (set to -1 for background event)<br> c5:Magnitude of the direct parent of the event (set to -10 if background event)<br> c6:Time of the event initiating the tree (set to own time if background)<br> c7:Magnitude of the event initiating the tree (set to own magnitude if background)<br> c8:N or offspring number of the event. (Events are leafs if N=0)</p> <p><br> 3.2. List of trees<br> ------------------</p> <p>53 x "*TopoTrees.dat" files obtained from simulations (after processing of *.Seq files. Files ending with "N0.99", "N0.50" obtained from 10^5 background events from files above. Files ending with "N0.500*" obtained from 10^7 simulations)<br> Each row represents an individual tree constituted by one or several causally connected events of the simulated branching forest. Files used to generate fig. 4 of ref. [1]</p> <p>Columns description (8 columns x data point):<br> c0:Maximum Depth of the tree<br> c1:Number of events in the tree<br> c2:Average depth of leaves<br> c3:total number of leaves<br> c4:Sum of the depth of all leaves (=c2*c3)<br> c5:(=0) not used<br> c6:Magnitude of root<br> c7:Maximum magnitude of an event inside the tree</p> <p><br> 4.3. Tree-size frequencies<br> --------------------------</p> <p>18 x "*TopoTrees.FK" files obtained from "*TopoTrees.dat". Contains the frequencies of tree-sizes. Each raw number correspond to a size. Each value corresponds to number of incidences of that size divided by total number of events (10^5 in all cases). Notice that last point is missing at size = max-length, freq.= 1.0 / total number of events. Files used to generate fig. 3 of ref. [1]</p> <p><br> ################<br> 4. LIST OF FILES<br> ################</p> <p>(copy of this text)<br> readme.txt<br> md5:2073cdbb4a0afd8ab96f8bfaef20579f 13 Kb</p> <p>4.1. Raw data (42 files)<br> ------------------------</p> <p>ETASbranch_b0.50r0.00N0.50.Seq<br> md5:bae21678db4fe575b06b69a5f32253d2 12.5 Mb<br> ETASbranch_b0.50r0.00N0.99.Seq<br> md5:76ea70ba94aeeed7ba3c358305f4b85b 1.3 Gb<br> ETASbranch_b0.50r0.05N0.50.Seq<br> md5:0ccf5af07efb9dd99d63a9675fd3069f 12.4 Mb<br> ETASbranch_b0.50r0.05N0.99.Seq<br> md5:77a7bfd490af54a5a3d15c4183e9e6f7 1.4 Gb<br> ETASbranch_b0.50r0.10N0.50.Seq<br> md5:f274717039f8f69ce57d5054577777af 12.4 Mb<br> ETASbranch_b0.50r0.10N0.99.Seq<br> md5:e18f4c26acfdff8d79a6223aa86867d2 1.3 Gb<br> ETASbranch_b0.50r0.15N0.50.Seq<br> md5:5f93c14a8d0f88fd021bc6d8a5fc7d6e 12.3 Mb<br> ETASbranch_b0.50r0.15N0.99.Seq<br> md5:906e09ecb6c6c18fdbcfdca3d9dbe225 1.3 Gb<br> ETASbranch_b0.50r0.20N0.50.Seq<br> md5:2467c2c7fafeddaa630074e991cb7767 12.4 Mb<br> ETASbranch_b0.50r0.20N0.99.Seq<br> md5:64c45a47b7f953d7088b10d4badd068c 1.3 Gb<br> ETASbranch_b0.50r0.25N0.50.Seq<br> md5:d65abaa4664b2412707b27b6e9143215 12.4 Mb<br> ETASbranch_b0.50r0.25N0.99.Seq<br> md5:8d7467b0bd80ce7e8cbcaf5dfe2725b7 1.2 Gb<br> ETASbranch_b0.50r0.30N0.50.Seq<br> md5:fd3af9802b3b66a623172bb2aafc0a0b 12.4 Mb<br> ETASbranch_b0.50r0.30N0.99.Seq<br> md5:2ad1b9b9c858638067af6068dfd25d59 1.3 Gb<br> ETASbranch_b0.50r0.35N0.50.Seq<br> md5:8cf36cc90a19f1283c838dee4849626b 12.5 Mb<br> ETASbranch_b0.50r0.35N0.99.Seq<br> md5:6b35085100561865ac9463edfb166d7f 1.4 Gb<br> ETASbranch_b0.50r0.40N0.50.Seq<br> md5:317b154c8ed74262b18c41762597d236 12.3 Mb<br> ETASbranch_b0.50r0.40N0.99.Seq<br> md5:c83dc299035928c9ae8a7f6d101ac9b8 1.2 Gb<br> ETASbranch_b0.50r0.45N0.50.Seq<br> md5:d3baccfbf347e555c30f3dfb1db1d9c0 12.4 Mb<br> ETASbranch_b0.50r0.45N0.99.Seq<br> md5:5bf42163acc2eae1eeae8549b850ccc9 1.1 Gb<br> ETASbranch_b0.50r0.50N0.50.Seq<br> md5:3ad7427725dfcf302c2dc2c519dad8c0 12.4 Mb<br> ETASbranch_b0.50r0.50N0.99.Seq<br> md5:7316fb3661b87215a1a7b1c11a54975b 1.3 Gb<br> ETASbranch_b0.50r0.55N0.50.Seq<br> md5:6dbc9846506675c61d110ae918165a6b 12.4 Mb<br> ETASbranch_b0.50r0.55N0.99.Seq<br> md5:5d4d007bf898512185dd2f87b715715e 1.4 Gb<br> ETASbranch_b0.50r0.60N0.50.Seq<br> md5:569c1c409ef618f518fe6fb6f41493a3 12.7 Mb<br> ETASbranch_b0.50r0.60N0.99.Seq<br> md5:e739613598e8d2530d108ff24e8c045a 1.1 Gb<br> ETASbranch_b0.50r0.65N0.50.Seq<br> md5:e95cead475c1eedb0618bebf1b548ec2 12.3 Mb<br> ETASbranch_b0.50r0.65N0.99.Seq<br> md5:8fe8b042085f261971d94bf12f96dc7e 1 Gb<br> ETASbranch_b0.50r0.70N0.50.Seq<br> md5:48824f1efdbc31c5bb4d4f799bc166d5 12.2 Mb<br> ETASbranch_b0.50r0.70N0.99.Seq<br> md5:2ead803394a4c3b04f1e22e9b8c5ba46 872.9 Mb<br> ETASbranch_b0.50r0.75N0.50.Seq<br> md5:8c5cf4753836076c677a74d831354f33 12.2 Mb<br> ETASbranch_b0.50r0.75N0.99.Seq<br> md5:91ed931aedfb365761799e0882fcfb86 1 Gb<br> ETASbranch_b0.50r0.80N0.50.Seq<br> md5:f93e1779bc5344ffd17021bb387bb373 11 Mb<br> ETASbranch_b0.50r0.80N0.99.Seq<br> md5:6b0fd7fcad20ac4467fecda9502e9954 75.6 Mb<br> ETASbranch_b0.50r0.85N0.50.Seq<br> md5:a5debe21de70204a490c75dd7c68657a 11 Mb<br> ETASbranch_b0.50r0.85N0.99.Seq<br> md5:f9ed3ad6db12ad4a612c3945ccf72f18 48.5 Mb<br> ETASbranch_b0.50r0.90N0.50.Seq<br> md5:4493eef2170b68052cf56636c63091d7 9.7 Mb<br> ETASbranch_b0.50r0.90N0.99.Seq<br> md5:70043ed6c84e3896268c46f77eabefd3 26.7 Mb<br> ETASbranch_b0.50r0.95N0.50.Seq<br> md5:874916faa95c63312aa27aecea45de8f 7.5 Mb<br> ETASbranch_b0.50r0.95N0.99.Seq<br> md5:bbc54464c2e3cbac64401978216ae993 11.7 Mb<br> ETASbranch_b0.50r1.00N0.50.Seq<br> md5:3ff467b894f46a4be0b0747c686edad5 5.8 Mb<br> ETASbranch_b0.50r1.00N0.99.Seq<br> md5:d4d5416d56ed3de6eb2702a8eeb0a0b5 5.8 Mb</p> <p><br> 3.2. List of trees (53 files)<br> -----------------------------</p> <p><br> ETASbranch_b1.00r0.00N0.500TopoTrees.dat<br> md5:a5834b8d047fd3446e6d83488422bb5b 1.8 Gb<br> ETASbranch_b1.00r0.00N0.99TopoTrees.dat<br> md5:34eb899782444475151073ed6fcfc6aa 18.6 Mb<br> ETASbranch_b1.00r0.05N0.50TopoTrees.dat<br> md5:cc5f0edfc31a5f15394444243560e0ad 18.6 Mb<br> ETASbranch_b1.00r0.05N0.99TopoTrees.dat<br> md5:58cc7e7e746051f9efbe510c164a2b09 18.6 Mb<br> ETASbranch_b1.00r0.10N0.500TopoTrees.dat<br> md5:52cb3a9a5aeb1e222743bcdad3ea3b3d 1.8 Gb<br> ETASbranch_b1.00r0.10N0.50TopoTrees.dat<br> md5:a68a69280c0129ee3554d5c97dd0fa47 18.6 Mb<br> ETASbranch_b1.00r0.10N0.99TopoTrees.dat<br> md5:307edf1353af5141f11d89cbab6c4d30 18.6 Mb<br> ETASbranch_b1.00r0.15N0.500TopoTrees.dat<br> md5:52807004c32bdc1a999a2aaf1ff91bb9 1.8 Gb<br> ETASbranch_b1.00r0.15N0.50TopoTrees.dat<br> md5:0bdb45b13ed0fd5c0fa1619310d71670 18.6 Mb<br> ETASbranch_b1.00r0.15N0.99TopoTrees.dat<br> md5:f26e351184921ed9a85c8993404a4718 18.6 Mb<br> ETASbranch_b1.00r0.20N0.500TopoTrees.dat<br> md5:5fd930a98e28d551c9c204d22c6f564a 1.8 Gb<br> ETASbranch_b1.00r0.20N0.50TopoTrees.dat<br> md5:4aaeb952e0ecb7d4fc4bd2fb7822c729 18.6 Mb<br> ETASbranch_b1.00r0.20N0.99TopoTrees.dat<br> md5:a30687662b269f447d7e4cc020bd3773 18.6 Mb<br> ETASbranch_b1.00r0.25N0.500TopoTrees.dat<br> md5:4168e6e70b247977213d2278b94d65f3 1.8 Gb<br> ETASbranch_b1.00r0.25N0.50TopoTrees.dat<br> md5:b360877be00088b52676ba4717377761 18.6 Mb<br> ETASbranch_b1.00r0.25N0.99TopoTrees.dat<br> md5:34d11b64c78c852d4f50de7b1265c9b7 18.6 Mb<br> ETASbranch_b1.00r0.30N0.500TopoTrees.dat<br> md5:8c1a8b07c5f2350646d415a687f84492 1.8 Gb<br> ETASbranch_b1.00r0.30N0.50TopoTrees.dat<br> md5:0c1bfccd8cd141090a0bfb0cc7ae1ccd 18.6 Mb<br> ETASbranch_b1.00r0.30N0.99TopoTrees.dat<br> md5:652f975754241fed317acba066cf339b 18.6 Mb<br> ETASbranch_b1.00r0.35N0.500TopoTrees.dat<br> md5:6bc2a3f7b4f7fd8a92595271d2ea425f 1.8 Gb<br> ETASbranch_b1.00r0.35N0.50TopoTrees.dat<br> md5:d1d4e8a467d8d445cd3f6ca27c3c8af3 18.6 Mb<br> ETASbranch_b1.00r0.35N0.99TopoTrees.dat<br> md5:e56eee0119b193898ea729b75c572617 18.6 Mb<br> ETASbranch_b1.00r0.40N0.500TopoTrees.dat<br> md5:370a2f6c670fd7c9b515e43891417b73 1.8 Gb<br> ETASbranch_b1.00r0.40N0.50TopoTrees.dat<br> md5:9f50c23e33cca983df58e2b17f29f8e5 18.6 Mb<br> ETASbranch_b1.00r0.40N0.99TopoTrees.dat<br> md5:d9971930b46838de09a7ccdc7cd9b459 18.6 Mb<br> ETASbranch_b1.00r0.45N0.500TopoTrees.dat<br> md5:cfef99746043d0718ad19e48ffdfeee4 1.8 Gb<br> ETASbranch_b1.00r0.45N0.50TopoTrees.dat<br> md5:44667f56360024e40b11be4f52ccf9c3 18.6 Mb<br> ETASbranch_b1.00r0.45N0.99TopoTrees.dat<br> md5:09b84c6f3c9dd58331c6f9ab4eec8f58 18.6 Mb<br> ETASbranch_b1.00r0.50N0.500TopoTrees.dat<br> md5:e9e880f24ffc9ba40db7c7f78d0f8d89 1.8 Gb<br> ETASbranch_b1.00r0.50N0.50TopoTrees.dat<br> md5:ce4835d532513bffd54723f285207898 1.9 Mb<br> ETASbranch_b1.00r0.50N0.99TopoTrees.dat<br> md5:4c935d76aee113c88f4a3348197f75f3 18.6 Mb<br> ETASbranch_b1.00r0.55N0.500TopoTrees.dat<br> md5:46cd9e8d508c15009e90a194a141008f 1.8 Gb<br> ETASbranch_b1.00r0.55N0.50TopoTrees.dat<br> md5:8e1cb9c364c2a275d10373b54dab6239 18.6 Mb<br> ETASbranch_b1.00r0.55N0.99TopoTrees.dat<br> md5:304b947c52e826a9b4cb68c38f007443 18.6 Mb<br> ETASbranch_b1.00r0.60N0.500TopoTrees.dat<br> md5:df8b7889e76694188b29f7fc16f6dce2 1.8 Gb<br> ETASbranch_b1.00r0.60N0.50TopoTrees.dat<br> md5:fc6c2110e139290ae9401765e8aae782 18.6 Mb<br> ETASbranch_b1.00r0.60N0.99TopoTrees.dat<br> md5:6af466cc527b7422f1f958bfc6a87d15 18.6 Mb<br> ETASbranch_b1.00r0.65N0.500TopoTrees.dat<br> md5:129abd6de7821da465265a485217156d 1.8 Gb<br> ETASbranch_b1.00r0.65N0.50TopoTrees.dat<br> md5:469fc2ae3768fe9ae0ab0b8fbfaf3051 18.6 Mb<br> ETASbranch_b1.00r0.65N0.99TopoTrees.dat<br> md5:b7148c414b243b1911de8543d25e3d38 18.6 Mb<br> ETASbranch_b1.00r0.70N0.500TopoTrees.dat<br> md5:3a3eed181ae6308c29f274d5e14a97df 1.8 Gb<br> ETASbranch_b1.00r0.70N0.50TopoTrees.dat<br> md5:ca7472f17916e7f8634a97c08ca96c58 18.6 Mb<br> ETASbranch_b1.00r0.70N0.99TopoTrees.dat<br> md5:8c2a4ab3800e8a8178be5d6e856f5b50 18.6 Mb<br> ETASbranch_b1.00r0.75N0.50TopoTrees.dat<br> md5:374796f34a3e25f711601161f8a34ae7 18.6 Mb<br> ETASbranch_b1.00r0.75N0.99TopoTrees.dat<br> md5:591f6ac31545f3e7558b4a5e151c80a5 18.6 Mb<br> ETASbranch_b1.00r0.80N0.50TopoTrees.dat<br> md5:473c6e9ac33cc6e8eabe0ed3c91b40b7 18.6 Mb<br> ETASbranch_b1.00r0.80N0.99TopoTrees.dat<br> md5:81f41fbce89c931f87890133b4c0f9f9 18.6 Mb<br> ETASbranch_b1.00r0.85N0.50TopoTrees.dat<br> md5:2c38db4208898d95c1c5b2ef0a94c930 18.6 Mb<br> ETASbranch_b1.00r0.85N0.99TopoTrees.dat<br> md5:4866f179b52ff5a6191d47e6d8ead5ce 18.6 Mb<br> ETASbranch_b1.00r0.90N0.50TopoTrees.dat<br> md5:f14a7f700c0c73743eb5747399abdcce 18.6 Mb<br> ETASbranch_b1.00r0.90N0.99TopoTrees.dat<br> md5:da4bd00aeefcbd1e67f0d7fe8fb1d8be 18.6 Mb<br> ETASbranch_b1.00r0.95N0.50TopoTrees.dat<br> md5:57b7f0bd901bb47ba3c253f801301716 18.6 Mb<br> ETASbranch_b1.00r0.95N0.99TopoTrees.dat<br> md5:6381354768603c6d2129c99df2a7798a 18.6 Mb</p> <p>4.3. Tree-size frequencies (18 files)<br> -------------------------------------</p> <p>ETASbranch_b1.00r0.00N0.99TopoTrees.FK<br> md5:5b6ccaf1c1218f101ed24c332bfefec3 612 Kb<br> ETASbranch_b1.00r0.10N0.30TopoTrees.FK<br> md5:f9f07bbd7be5377e1589101e85640149 468 B<br> ETASbranch_b1.00r0.20N0.30TopoTrees.FK<br> md5:6c7e40f8b93c7a3e62f2c105f0e7a89b 558 B<br> ETASbranch_b1.00r0.20N0.99TopoTrees.FK<br> md5:158884c9bc4afa10c8ebb2e7b516a421 3.3 Mb<br> ETASbranch_b1.00r0.30N0.30TopoTrees.FK<br> md5:14d837b9763b9a54e64311e832e29f04 846 B<br> ETASbranch_b1.00r0.30N0.99TopoTrees.FK<br> md5:90b8b5a38a83cb4b4c833b605cf6b38e 2.1 Mb<br> ETASbranch_b1.00r0.40N0.30TopoTrees.FK<br> md5:1f198a7c08078066e7fa57ce9ebda8eb 3 Kb<br> ETASbranch_b1.00r0.40N0.99TopoTrees.FK<br> md5:229b837e7950fe85ea1c51be8e3f457e 3.3 Mb<br> ETASbranch_b1.00r0.50N0.30TopoTrees.FK<br> md5:6ead8bff09c3c2687c526648bfec6ab3 16 Kb<br> ETASbranch_b1.00r0.60N0.30TopoTrees.FK<br> md5:38c5cb9f544367ff9bdab766c4bcf03f 112 Kb<br> ETASbranch_b1.00r0.60N0.99TopoTrees.FK<br> md5:2591d4e2dd1c2051c0b7d96f423c526a 106.5 Mb<br> ETASbranch_b1.00r0.70N0.30TopoTrees.FK<br> md5:4f7741314e1a0d0b1c1733532d909277 7.5 Mb<br> ETASbranch_b1.00r0.80N0.30TopoTrees.FK<br> md5:3c4240e79ee19c03afac91d631f38cb8 602 Kb<br> ETASbranch_b1.00r0.80N0.30TopoTrees.FK<br> md5:3c4240e79ee19c03afac91d631f38cb8 602 Kb<br> ETASbranch_b1.00r0.90N0.30TopoTrees.FK<br> md5:914a782fa2e73c0bc5a6e70bbb2afec9 1.3 Mb<br> ETASbranch_b1.00r0.90N0.99TopoTrees.FK<br> md5:cc165045d4d9f25c0f9c30c0ec71759f 864 Kb<br> ETASbranch_b1.00r0.99N0.30TopoTrees.FK<br> md5:a08cfc27a0e42d54f207ea05b7210714 187 Kb<br> ETASbranch_b1.00r0.99N0.99TopoTrees.FK<br> md5:a15681be56f870a95fe62794b11524df 365 Kb</p> <p><br> 4.4 Known missing/broken files<br> ------------------------------</p> <p>ETASbranch_b1.00r0.50N0.50TopoTrees.dat<br> ETASbranch_b1.00r0.10N0.99TopoTrees.FK<br> ETASbranch_b1.00r0.20N0.99TopoTrees.FK<br> ETASbranch_b1.00r0.50N0.99TopoTrees.FK<br> ETASbranch_b1.00r0.70N0.99TopoTrees.FK<br> ETASbranch_b1.00r0.05N0.500TopoTrees.dat<br> ETASbranch_b1.00r0.70N0.500TopoTrees.dat<br> ETASbranch_b1.00r0.75N0.500TopoTrees.dat<br> ETASbranch_b1.00r0.80N0.500TopoTrees.dat<br> ETASbranch_b1.00r0.85N0.500TopoTrees.dat<br> ETASbranch_b1.00r0.90N0.500TopoTrees.dat<br> ETASbranch_b1.00r0.95N0.500TopoTrees.dat<br> </p>
Rapid and real-time identification of fungi up to the species level with long amplicon Nanopore sequencing from clinical samples
<p>Samples collected from fungal cultures, skin of dogs and ZymoBIOMICS<sup>TM </sup>mock community (which includes <em>Saccharomyces cerevisiae</em> and <em>Cryptococcus neoformans</em>). The amplicons length of the fungal cultures and ZymoBIOMICS<sup>TM </sup>mock community is 3,5 Kb and 6 Kb, while the <em>Malassezia spp</em> samples used as control is 3,5 Kb. The amplicons length of the four samples from the skin is 3,5 Kb.</p>
GenBank + BOLD CO1 Eukaryotic representative sequence set
<p>This is a representative sequence set for cytochrome oxidase subunit 1 (CO1 or COI) combining all available eukaryotic CO1 sequences from GenBank and BOLD, clustered at 99% similarity.</p> <p> </p> <p>TODO:</p> <p>generate and add 7-level taxonomies for each sequence in this rep set.</p>
A Bayesian Phylogenetic Hidden Markov Model for B Cell Receptor Sequence Analysis
<p>simulation and PC64/VRC01 input/output data files</p>
Dataset used for "Somatic hypermutation analysis for improved identification of B cell clonal families from next-generation sequencing data"
<p>Each simulated dataset was generated using the AbSim R package (version 0.2.6) in a B cell single-lineage fashion. Each B cell clone simulation begins with a random selection from sets of IGHV, IGHD, and IGHJ germline sequences to produce a unique V(D)J recombination event. Then, clones are made by introducing mutations using a local nucleotide context-dependent model (S5F model) along a phylogenetic tree in which branching events occur stochastically. </p>
Dataset for NMR quadrature echo and T1 saturation recovery pulse sequences underlying the publication 'On the quantification of solid phases in hydrated cement paste by 1H nuclear magnetic resonance relaxometry'
<p>This record comprises the datasets of combined 1H NMR quadrature echo and T1 saturation recovery pulse sequences underlying the publication “On the quantification of solid phases in hydrated cement paste by 1H nuclear magnetic resonance relaxometry” by Robert Schulte Holthausen & Peter J. McDonald, Cement and Concrete Research, https://doi.org/10.1016/j.cemconres.2020.106095.</p> <p><br> In this work different solid phases, important to cement paste hydration, are investigated with low-field bench top 1H nuclear magnetic resonance with a view to developing an alternate characterisation methodology that requires minimal invasive or destructive sample preparation.</p> <p><br> A combination of the well-established quadrature echo pulse sequence with variable pulse gap together with a T1 saturation recovery quadrature echo pulse sequence is used.</p>
Fig. 12 in A revision of the Thyropygus allevatus group. Part V: Nine new species of the extended opinatus subgroup, based on morphological and DNA sequence data (Diplopoda: Spirostreptida: Harpagophoridae)
Fig. 12. Known distribution of the species of the T. opinatus subgroup.
Characterizing and classifying neuroendocrine neoplasms through microRNA sequencing and data mining
<p>Neuroendocrine neoplasms (NENs) are clinically diverse and incompletely characterized cancers that are challenging to classify. MicroRNAs (miRNAs) are small regulatory RNAs that can be used to classify cancers. Recently, a morphology-based classification framework for evaluating NENs from different anatomic sites was proposed by experts, with the requirement of improved molecular data integration. Here, we compiled 378 miRNA expression profiles to examine NEN classification through comprehensive miRNA profiling and data mining. Following data preprocessing, our final study cohort included 221 NEN and 114 non-NEN samples, representing 15 NEN pathological types and five site-matched non-NEN control groups. Unsupervised hierarchical clustering of miRNA expression profiles clearly separated NENs from non-NENs. Comparative analyses showed that miR-375 and miR-7 expression is substantially higher in NEN cases than non-NEN controls. Correlation analyses showed that NENs from diverse anatomic sites have convergent miRNA expression programs, likely reflecting morphologic and functional similarities. Using machine learning approaches, we identified 17 miRNAs to discriminate 15 NEN pathological types and subsequently constructed a multi-layer classifier, correctly identifying 217 (98%) of 221 samples and overturning one histologic diagnosis. Through our research, we have identified common and type-specific miRNA tissue markers and constructed an accurate miRNA-based classifier, advancing our understanding of NEN diversity.</p>
Next-generation sequencing of newborn screening genes: The accuracy of short-read mapping
<p>We examine the effect of high homology genomic regions on the mapping of genes related to newborn screening while taking different read lengths and patient's ethnic background into consideration.</p>
Amplicon sequence variants by sample table from Antarctic methane seeps
<p>Antarctica is estimated to contain as much as a quarter of earth's marine methane, however we have not discovered an active Antarctic methane seep limiting our understanding of the methane cycle. In 2011, an expansive (70m x 1m) microbial mat formed at 10m water depth in the Ross Sea, Antarctica and we carried out 16S rRNA gene analysis on samples collected one year and five years after the methane seep formed. The data set attached is the resulting Amplicon Sequence Variant table by sample that we used to track the community composition change during this time and in comparison to other sampling in the McMurdo sound region. </p>
Data from: Accumulation curves of environmental DNA sequences predict coastal fish diversity in the Coral Triangle
Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments particularly for vertebrates in species-rich regions. Yet, this method requires the completeness of a reference database, i.e. a list of DNA sequences attached to each species, which is never met. As an alternative, a diversity of Operational Taxonomic Units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1,531 fish OTUs while 1,611 fish species are recorded in the region. Besides, we also accurately predict (R² = 0.92) the distribution of species richness among fish families from OTU-based asymptotes. Thus, the multi-model framework of OTU accumulation curves extends the use of eDNA metabarcoding in ecology, biogeography and conservation.
Data from: RapidRat: development, validation and application of a genotyping-by-sequencing panel for rapid biosecurity and invasive species management
<p>Invasive alien species (IAS) are among the main causes of global biodiversity loss. Invasive brown (Rattus norvegicus) and black (R. rattus) rats, in particular, are leading drivers of extinction on islands, especially in the case of seabirds where >50% of all extinctions have been attributed to rat predation. Eradication is the primary form of invasive rat management, yet this strategy has resulted in a ~10-38% failure rate on islands globally. Genetic tools can help inform IAS management, but such applications to date have been largely reactive, time-consuming, and costly. Here, we developed a Genotyping-in-Thousands by sequencing (GT-seq) panel for rapid species identification and population assignment of invasive brown and black rats (RapidRat) in Haida Gwaii, an archipelago comprising ~150 islands off the central coast of British Columbia, Canada. We constructed an optimized panel of 443 single nucleotide polymorphisms (SNPs) using previously generated double-digest restriction-site associated DNA (ddRAD) genotypic data (27,686 SNPs) from brown (n=295) and black rats (n=241) sampled throughout Haida Gwaii. The informativeness of this panel for identifying individuals to species and island of origin was validated relative to the ddRAD results; in all comparisons, admixture coefficients and population assignments estimated using RapidRat were consistent. To demonstrate application, 20 individuals from novel invasions of three islands (Agglomerate, Hotspring, Ramsay) were genotyped using RapidRat, all of which were confidently assigned (>98.5% probability) to Faraday and Murchison Islands as putative source populations. These results indicated that a previous eradication on Hotspring Island was conducted at an inappropriate geographic scale; future management should expand the eradication unit to include neighboring islands to prevent re-invasion. Overall, we demonstrated that RapidRat is an effective tool for managing invasive rat populations in Haida Gwaii and provided a clear framework for GT-seq panel development for informing biodiversity conservation in other systems.</p>
RNA sequences for Aedes species, Dengue, and Chikungunya viruses
<p>There are arthropod-borne disease outbreaks as a result of pathogen influx including arboviruses which are transmitted by strains of <em>Aedes</em> species that occur periodically in varying spots on the globe. The aim of this study was to determine phylogenetic relationship of <em>Aedes</em> mosquitoes, Dengue, and Chikungunya viruses along the Coastline of Kenya based on sequences of:</p> <ol> <li>mitochondria nicotinamide adenine dehydrogenase sub unit 4 gene for Aedes species.</li> <li>non-structural protein 5 gene for Dengue virus</li> <li>non-structural protein 1 gene for Chikungunya virus</li> </ol>
Models from: Exploring ensemble applications for multi-sequence myocardial pathology segmentation
<p>Trained models for the MyoPS2020 challenge http://www.sdspeople.fudan.edu.cn/zhuangxiahai/0/MyoPS20/</p> <p>As described in the publication: "Exploring ensemble applications for multi-sequence myocardial pathology segmentation"</p> <p>Source code available at: https://github.com/chfc-cmi/miccai2020-myops</p>
Heterogeneity in the rate of molecular sequence evolution substantially impacts the accuracy of detecting shifts in diversification rates
<p>As species richness varies along the tree of life, there is a great interest in identifying factors that affect the rates by which lineages speciate or go extinct. To this end, theoretical biologists have developed a suit of phylogenetic comparative methods that aim to identify where shifts in diversification rates had occurred along a phylogeny and whether they are associated with some traits. Using these methods, numerous studies have predicted that speciation and extinction rates vary across the tree of life. In this study we show that asymmetric rates of sequence evolution rates lead to systematic biases in the inferred phylogeny, which in turn lead to erroneous inferences regarding lineage diversification patterns. The results demonstrate that as the asymmetry in sequence evolution rates increases, so does the tendency to select more complicated models that include the possibility of diversification rate shifts. These results thus suggest that any inference regarding shifts in diversification pattern should be treated with great caution, at least until any biases regarding the molecular substitution rate have been ruled out.</p>
Global Characterization of Megakaryocytes in Bone Marrow, Peripheral Blood, and Cord Blood by Single-cell RNA Sequencing
<p><span><span>Megakaryocytes (MK) are mainly derived from bone marrow (BM) and are mainly involved in platelet production. Recent studies have shown that MK derived from BM may have immune function, and that MK from peripheral blood (PB) are associated with prostate cancer. We analyzed more than 1.2 million single-cell transcriptome data from 132 samples of PB, BM, and cord blood (CB) from healthy individuals and patients, and obtained 4474 MK single cell and 14 MK subtypes. We found that MK were widely distributed and the amount of MK in PB was more than that in BM and there were specificity MK subtypes in PB. We found classical MK1 with typical MK characteristics and non-classical MK2 closely related to immunity which was the most common subtype in BM and CB. Classical MK1 was closely related to Non-Small Cell Lung Cancer (NSCLC) and has diagnostic ability. MK2 may have potential adaptive immune function and play a role in tumor NSCLC and autoimmune diseases Systemic Lupus Erythematosus. This study deepened our understanding of MK and suggested that MK had potential immune functions and was involved in various diseases.</span></span></p>
Sex-linked markers by genome-wide RAD sequencing to identify XX/XY Sex Chromosomes in the spiny frog (Quasipaa boulengeri)
<p><span>We use genotyping by sequencing as an approach to identify sex-linked markers in the spiny frog <i>Quasipaa boulengeri</i> with 43 wild-collected adults from a single site. The GBS methodology identified 2 loci on sex differences in allele frequencies, 50 loci on sex differences in heterozygosity, and 523 loci on male-limited occurrence, altogether associated with males heterogamety, indicating an XX-XY system. The sex specificity of five markers was further validated by PCR amplification with a large number of additional individuals from 26 various populations in this species. A total of 27 sex linkage markers were matched to Dmrt1 gene, a ubiquitous role in sex determination and differentiation from flies and nematodes to mammals. Chromosome 1, that harboring Dmrt1, has further been assigned to a highly potential candidate sex chromosome in anurans. Five sex-linked SNP makers explored 3 sex reversals out of 133 individuals here, sparsely showing sex reversal detected in wild amphibian populations. </span></p>
A genomic data set of single‐nucleotide polymorphisms (SNPs) generated by ddRAD tag sequencing in Q. petraea (Matt.) Liebl. populations from Central-Eastern Europe and Balkan Peninsula
<p>This genomic dataset provides highly variable single-nucleotide polymorphism (SNP) markers from georeferenced natural <em>Quercus petraea</em> (Matt.) Liebl. populations collected in Bulgaria, Hungary, Romania, Serbia, Bosnia and Herzegovina, Kosovo and Albania. These SNP loci can be used to assess genetic diversity, differentiation, population structure, and can also be used to detect signatures of selection and local adaptation.</p>
The influence of human genetic variation on Epstein-Barr virus sequence diversity
<p>This project is the first attempt to apply a "genome-to-genome" approach to investigate the impact of the host genetic pressure on the genome of a member of the <em>Herpesviridae</em> family. Namely, 285 pairs of human and EBV genomes were sequenced and multiple GWASes between human and EBV variations were performed. This repository contains the results of downstream analysis on the pathogen-side. The variant calling data was produced from read alignment using BWA mem using GATK HC, SNVer, VarScan2, BCFtools, freebayes and the intersection of the sets of variation from the first three.</p> <ul> <li><strong>The "covstats" files</strong> contains statistics about the read alignment.</li> <li><strong>The tarball SHCS_EBV_variant_call.tar.gz</strong> contains all compressed VCF files.</li> <li><strong>The tarball SHCS_EBV_variant_matrices_stats.tar.gz</strong> contains all matrices used as traits in the GWASes, as well as a variety of statistics.</li> </ul> <p>The pipeline used to generate this data is publically available here: <a href="https://gitlab.com/ezlab/vir_var_calling/">https://gitlab.com/ezlab/vir_var_calling/</a></p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.