Find research datasets worth reusing

opencc-by-4.0Jul 2022View details →

Air quality data from the article "Typhoon-associated air quality over the Guangdong–Hong Kong–Macao Greater Bay Area, China: machine-learning-based prediction and assessment"

This dataset consists of 26 files. The descriptions of the files are as follows: <ul> <li>aqi_TY.csv, pm25_TY.csv, pm10_TY.csv, so2_TY.csv, no2_TY.csv and o3_TY.csv are the observed values of AQI and concentrations of PM2.5, PM10, SO2, NO2 and O3 of 36 monitoring stations used in model establish stage on TY days. The time range is June 2014 to December 2020.</li> <li>aqi_NTY.csv, pm25_NTY.csv, pm10_NTY.csv, so2_NTY.csv, no2_NTY.csv and o3_NTY.csv are the observed values of AQI and concentrations of PM2.5, PM10, SO2, NO2 and O3 of 36 monitoring stations used in model establish stage on NTY days. The time range is June 2014 to December 2020.</li> <li>station_info.csv is the detailed information of the 36 monitoring stations used in model establish stage, including station number, city, longitude and latitude.</li> <li>aqi_TY_testing.csv, pm25_TY_testing.csv, pm10_TY_testing.csv, so2_TY_testing.csv, no2_TY_testing.csv and o3_TY_testing.csv are the observed values of AQI and concentrations of PM2.5, PM10, SO2, NO2 and O3 of 3 monitoring stations used for testing the model on TY days. The time range is June 2014 to December 2020.</li> <li>aqi_NTY_testing.csv, pm25_NTY_testing.csv, pm10_NTY_testing.csv, so2_NTY_testing.csv, no2_NTY_testing.csv and o3_NTY_testing.csv are the observed values of AQI and concentrations of PM2.5, PM10, SO2, NO2 and O3 of 3 monitoring stations used for testing the model on NTY days. The time range is June 2014 to December 2020.</li> <li>sta_testing.csv is the detailed information of the 3 monitoring stations used for testing the model, including station number, city, longitude and latitude.</li> </ul>

opencc-by-4.0Sep 2022View details →

Dataset associated with article: Self-sufficient seismic boxes for monitoring glacier seismology in Greenland

Dataset associated with article: Self-sufficient seismic boxes for monitoring glacier3 seismology in Greenland Contains: - Seismic data of both SG-boxes and regular geophones from Gornergletscher fieldtest, 2021( Seismic_Data_Gorner_Fieldtest.zip)           --> SG-box data naming: GO"station_number"SG               --> Geophone data naming: GO"station_number"GP - Weather data Gornergletscher fieldtest from Monte Rosa, Meteo Swiss Weather station ( Weather_data_Gorner_Fieldtest_2021.zip)               --> 1hr wind averages               --> 1hr temperature averages - MSR logger data from SG-boxes from Gornergletscher fieldtest, 2021. Every 5 min these log battery power, tilt (along three axes, temperature and humidity inside the box and light strength on two sides of the SG-box. ( MSR_logger_data_SGboxes_Gorner_Fieldtest.zip ) - Seismic data of SG box (Sensor code BSM) next to weather station first acquisition Greenland 2021 ( Seismic_Data_SG_Box_first_acquisition_Greenland_2021.zip)                 --> .pri0 is East component, .pri1 is North component, .pri2 is Vertical component. - Weather data from weather station next to SG-box (sensor code BSM) during first acquisition Greenland 2021 ( Weather_station_data_Greenland_2021.zip)                  --> The weather station logs a value every two hours.

Datasets of the article "Sub-millisecond Photoinduced Dynamics of Free and EL222-bound FMN by Stimulated Raman and Visible Absorption Spectroscopies"

Femtosecond-stimulated Raman spectra (FSRS) and visible transient absorption (visTA) spectra of free FMN (flavin mononucleotide), FMN bound to EL222-WT, and FMN bound to EL222-C78A. The dataset includes also the lifetime distributions, decay-associated difference spectra (DADS), and evolution-associated difference spectra (EADS). Please check the README file for more information. If you use any of these data, please cite:  Liu, Y.; Chaudhari, A.S.; Chatterjee, A.; Andrikopoulos, P.C.; Picchiotti, A.; Rebarz, M.; Kloz, M.; Lorenz-Fonfria, V.A.; Schneider, B.; Fuertes, G. Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies. Biomolecules 2023, 13, 161. https://doi.org/10.3390/biom13010161

Dataset related to the article "Purinergic Receptor P2Y2 Stimulation Averts Aortic Valve Interstitial Cell Calcification and Myofibroblastic Activation"

This record contains raw data related to the article "Purinergic Receptor P2Y2 Stimulation Averts Aortic Valve Interstitial Cell Calcification and Myofibroblastic Activation" Rationale-Calcific aortic valve stenosis (CAVS) is a pathological condition of the aortic valve with a prevalence of 3% in the general population. It is characterized by massive rearrangement of the extracellular matrix, mostly due to the accumulation of fibro-calcific deposits driven by valve interstitial cells (VIC), and no pharmacological treatment is currently available. The aim of this study was to evaluate the effects of P2Y2 receptor (P2RY2) activation on fibro-calcific remodeling of CAVS. Methods-We employed human primary VICs isolated from CAVS leaflets treated with 2-thiouridine-5'-triphosphate (2ThioUTP, 10 µM), an agonist of P2RY2. The calcification was induced by inorganic phosphate (2 mM) and ascorbic acid (50 µg/mL) for 7 or 14 days, while the 2ThioUTP was administered starting from the seventh day. 2ThioUTP was chronically administered for 5 days to evaluate myofibroblastic activation. Results-P2RY2 activation, under continuous or interrupted pro-calcific stimuli, led to a significant inhibition of VIC calcification potential (p < 0.01). Moreover, 2ThioUTP treatment was able to significantly reduce pro-fibrotic gene expression (p < 0.05), as well as that of protein α-smooth muscle actin (p = 0.004). Conclusions-Our data suggest that P2RY2 activation should be further investigated as a pharmacological target for the prevention of CAVS progression, acting on both calcification and myofibroblastic activation.

Dataset related to the article "Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR"

This record contains raw data related to the article "Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR" Circulating microRNAs (miRNA) have been proposed as specific biomarkers for several diseases. Quantitative Real-Time PCR (RT-qPCR) is the gold standard technique currently used to evaluate miRNAs expression from different sources. In the last few years, digital PCR (dPCR) emerged as a complementary and accurate detection method. When dealing with gene expression, the first and most delicate step is nucleic-acid isolation. However, all currently available protocols for RNA extraction suffer from the variable loss of RNA species due to the chemicals and number of steps involved, from sample lysis to nucleic acid elution. Here, we evaluated a new process for the detection of circulating miRNAs, consisting of sample lysis followed by direct evaluation by dPCR in plasma from healthy donors and in the cardiovascular setting. Our results showed that dPCR is able to detect, with high accuracy, low-copy-number as well as highly expressed miRNAs in human plasma samples without the need for RNA extraction. Moreover, we assessed a known myocardial infarction-related miR-133a in acute myocardial infarct patients vs. healthy subjects. In conclusion, our results show the suitability of the extraction-free quantification of circulating miRNAs as disease markers by direct dPCR.

Informes de casos de estudio finales en hábitats humanos (anexo del libro: La visión sistémica del ambiente construido, 2024). [Data sets for the article: The Habitat Intervention Design Process -Part II]

From the book: Estos informes están escritos en español por los estudiantes que se mencionan en cada informe, quienes son los autores. Cada uno de estos seis documentos contiene casos de estudio y muestra la implementación de la metodología del 'Proceso de Diseño de Intervención del Hábitat' (IDP) en cada uno de los 6 casos mencionados en el libro (Capítulo 2.5 y Capítulo 3.2) (Libro: La visión sistémica del ambiente construido).  Estos hacen parte de la Fase 2 Fundamentos Prácticos del Modelo del proyecto de investigación "Modelo Pedagógico para la Enseñanza del Diseño de Intervención del Hábitat en Programas de Educación Superior".  From the article: These reports are Spanish-written. Each of these six documents contains one of the case studies and shows the implementation of the 'Habitat Intervention Design Process' (IDP) methodology in each of the 6 case studies mentioned in the article "The Habitat Intervention Design Process -Part II: A Transdisciplinary Model in the Pedagogy of the Design of the Built Environment" published in The International Journal of Design Education in its 2023 version. Find it here: <a href="https://doi.org/10.18848/2325-128X/CGP/v17i02/155-195">https://doi.org/10.18848/2325-128X/CGP/v17i02/155-195 </a>

Dataset related to article "Archival skin biopsy specimens as a tool for miRNA-based diagnosis: Technical and post-analytical considerations".

microRNA raw data

Dataset for article titled "Assessing continuum plasticity postulates with grain stress and local strain measurements in triaxially compressed sand"

Dataset containing raw and processed data from experiments presented in the article titled "Assessing continuum plasticity postulates with grain-scale stress and strain measurements in triaxially-compressed sand". An enclosed PDF file describes the data.

Raw GBIF data used in the article "Climate-induced range shifts drive adaptive response via spatio-temporal sieving of alleles"

This table contains the raw (unfiltered) species occurrence data for Dianthus sylvestris complied from GBIF. A filtered subset of this data was combined with other data sources to define the input for the species distribution models used in the article "Climate-induced range shifts drive adaptive response via spatio-temporal sieving of alleles".

opencc-by-4.0Jan 2020View details →

Datasets of the article "Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations"

Experimental time-resolved femtosecond-stimulated Raman spectra (FSRS) and visible transient absorption (visTA) spectra of flavin mononucleotide (FMN) in water, MES pH=6, and TRIS pH=8  buffers (DATA files). The dataset includes also the amplitudes and evolution-associated difference spectra (EADS) arising from global kinetic analysis using a sequential model.  In the DATA files, the first column contains the Stokes shift (in cm-1) or wavelength (in nm), and the first row contains the time delays (in seconds). In the AMPLITUDES files, the first column contains the time delays (in seconds), and the first row contains the lifetimes (in seconds). In the EADS files, the first column contains the wavenumbers (in cm-1) or wavelength (in nm), and the first row contains the lifetimes (in seconds). Note that the FSRS spectra have been normalized to 1. If you use these datasets please cite: Andrikopoulos, P. C.; Liu, Y.; Picchiotti, A.; Lenngren, N.; Kloz, M.; Chaudhari, A. S.; Precek, M.; Rebarz, M.; Andreasson, J.; Hajdu, J.; Schneider, B.; Fuertes, G.* Femtosecond-to-Nanosecond Dynamics of Flavin Mononucleotide Monitored by Stimulated Raman Spectroscopy and Simulations. Phys. Chem. Chem. Phys. PCCP 2020, 22, 6538–6552.

dryad36/100

Dataset related to article: Differential effect of climate of origin and cultivation climate on structural and biochemical plant traits

Exploring patterns and causes of intraspecific trait variation is crucial for a better understanding of the effects of climate change on plant populations and ecosystems. However, our current understanding of the intraspecific trait variation is mainly based on structural (morphological) traits, and we have limited knowledge on patterns and causes of variation in biochemical traits (e.g., leaf pigments), which are also crucial for plant adaptation. As a result, we also do not know how similar the climatic effects on structural versus biochemical traits are. Using plant traits from 110 genotypes representing 11 Festuca rubra populations grown in 4 different climates, we studied trait covariation among structural traits (linked to fitness, resource use, gas exchange, and reproduction) and biochemical traits (linked to photosynthesis, photoprotection, and oxidative stress). We also disentangled the relative role of the climate of origin and the climate of cultivation in the structural versus biochemical traits and tested for adaptive plasticity in the traits. We found that 1) biochemical traits did not covary with structural traits and represent independent 'photoharvesting – photoprotection' strategy dimension of functional variation; 2) interactive effects of climate of origin and cultivation were more pronounced for biochemical than structural traits. 3) Trait plasticity was affected by the climate of origin (precipitation and temperature as well as their interaction); 4) F. rubra showed both adaptive and mal-adaptive plasticity, and adaptiveness depended upon trait type, cultivation climate, and climate of origin. Overall, our results suggest that structural and biochemical plant traits respond differentially to climate and thus the response of one group of traits cannot be predicted from the other. Responses are also strongly determined by interactions between the climate of origin and cultivation. Thus, more studies on variation in biochemical traits, their correspondence to other traits, and their variation with climate are needed.

opencc-zeroFeb 2023View details →

Raw Data for the article: Neurological Screening in Elderly Liver Transplantation Candidates: A Single Center

Background: Cerebral small vessels disease (cSVD) is an age-related disorder and risk factor for stroke and cognitive/motor impairments. Neurological complications (NCs) are among the causes of adverse outcomes in older liver transplant recipients. This study sought to determine whether cSVD predicts acute NCs in over 65-year-old liver transplant patients. Methods: Data were collected, from a retrospective medical chart review, of 22 deceased donor liver transplant recipients aged 65 years or older with a pre-operative brain magnetic resonance imaging (MRI). We used the Fazekas score (0-3) as a quantitative measurement of the vascular lesion load seen in the MRI. We analyzed all post-operative acute NCs occurring during the hospital stay and any other non-NC. Results: cSVD was recognized in all patients. Neurological complications (NCs) occurred in 18.1% of patients with toxic-metabolic encephalopathy the most frequent diagnosis (13.64%). More severe cSVD was associated with seizures (p = 0.0362), longer hospital stay (p 0.0299), and disability (p 0.0134). In our elderly cohort, hepatic encephalopathy (HE) (p 0.0287) and ascites (p 0.0270) were predictors of NCs after liver transplantation. Ascites and/or variceal bleeding and severity of liver disease were associated with adverse post-operative outcomes. The small sample size limited the statistical analysis power. Conclusions: We present the preliminary data of a single-center retrospective study aimed at understanding the cSVD role on NCs and non-NCs after a liver transplantation in elderly patients. This would encourage a more appropriate multicenter prospective study that will definitely confirm if a neurological screening in old age liver transplant candidates is appropriate.

Raw Data for the article: Impact of the Organizational Model Adopted during the COVID-19 Pandemic on the Perceived Safety of Intensive Care Unit Staff

Background: The SARS-CoV-2 pandemic had a devastating health, social, and economic effect on the population. Organizational, technical and structural operations aimed at protecting staff, outpatients and inpatients were implemented in an Italian hospital with a COVID-19 dedicated intensive care unit. The impact of the organizational model adopted on the perceived safety among staff was evaluated. Methods: Descriptive, structured and voluntary, anonymous, non-funded, self-administered cross-sectional surveys on the impact of the organizational model adopted during COVID-19 on the perceived safety among staff. Results: Response rate to the survey was 67.4% (153 completed surveys). A total of 91 (59%) of respondents had more than three years of ICU experience, while 16 (10%) were employed for less than one year. Group stratification according to profession: 74 nurses (48%); 12 medical-doctors (7%); 11 physiotherapists (7%); 35 nurses-aides (22%); 5 radiology-technicians (3%); 3 housekeeping (1%); 13 other (8%). The organizational model implemented at ISMETT made them feel safe during their workday. A total of 113 (84%) agreed or strongly agreed with the sense of security resulting from the implemented measures. A vast majority of respondents perceived COVID-19 as a dangerous and deadly disease (94%) not only for themselves but even more as vectors towards their families (79%). A total of 55% of staff took isolation measures and moved away from their home by changing personal habits. The organizational model was perceived overall as appropriate (91%) to guarantee their health. Conclusion: The vast majority of respondents perceived the overall model applied during an unexpected, emergency situation as appropriate.

Raw Data for the article: Analysis of the Specific Immune Response after the Third Dose of mRNA COVID-19 Vaccines in Organ Transplant Recipients: Possible Spike-S1 Reactive IgA Signature in Protection from SARS-CoV-2 Infection

Background: Several studies have indicated that anti-SARS-CoV-2 mRNA vaccinations are less effective in inducing robust immune responses among solid organ transplant recipients (SOTRs) compared with the immunocompetent. The third dose of vaccine in SOTRs showed promising results of immunogenicity, even though clinical studies have suggested that immunocompromised subjects are less likely to build a protective immune response against SARS-CoV-2 resulting in lower vaccine efficacy for the prevention of severe COVID-19. Methods: Serological IgG and IgA were analyzed through CLIA or ELISA, respectively, while Spike-specific T cells were detected by ELISpot assay after the second and third dose of vaccine in 43 SOTRs. Results: The third dose induced an improvement in antibody response against SARS-CoV-2. We also reported a strong correlation between specific humoral and cellular responses after the third dose, even though we did not see significant changes in the magnitude of the SARS-CoV-2-specific T cell response. SOTRs who contracted the SARS-CoV-2 infection after the third dose, despite eliciting a positive IgG response, failed to mount an anti-Spike-S1 IgA response, both after the third dose and after SARS-CoV-2 infection. Conclusions: We can conclude that serum IgA detection can be helpful, along with IgG detection, for the evaluation of vaccine efficacy, principally in fragile subjects at high risk of infection.

Raw Data for the article: Right vs left portal branch puncture in TIPS creation with controlled expansion covered stent: comparison of hemodynamic and clinical outcomes

Purpose: To retrospectively compare outcomes of TIPS performed by puncturing left portal vein (LPV) vs right portal vein (RPV) to access the portal system. Materials and methods: One hundred ninety-three consecutive patients underwent TIPS with controlled expansion covered stent by using the LPV (37 patients) or the RPV (156 patients). Patients were followed until the last clinical evaluation, liver transplantation, or death. Results: Demographics and clinical characteristics of the two groups were comparable. The median follow-up was 9.6 months (range 0.1-50.6). Portosystemic pressure gradient (PSG) before TIPS 15.7 mmHg ± 4.7 in RPV group (RPVG) vs 15.4 mmHg ± 4.5 in LPV group (LPVG) (p = 0.725). After TIPS, PSG 6.3 mmHg ± 2.8 in RPVG vs 6.2 mmHg ± 2.2 (p = 0.839). In LPVG, the stent was dilated to 8-mm in 95% of patients vs 77% of RPVG (p = 0.015). Two (5.4%) and 22 (14%) patients underwent TIPS revision in LPVG and RPVG (p = 0.15). The incidence of overt HE was 13% in LPVG and 24% in RPVG (p = 0.177). Rebleeding occurred in 3 of 49 patients (6%) with variceal bleeding as an indication: 2/41 patients (4.9%) in RPVG vs 1/8 patients (12.5%) in LPVG (p = 0.417). Among 126 patients with refractory ascites 20 patients (15.9%) needed paracentesis 3 months after the procedure: 18/101 patients (17.8%) in RPVG vs 2/25 patients (8%) in LPVG (p = 0.231). Thirty-seven patients (19%) died: 32 (21%) in RPVG and 5 (14%) in LPVG (p = 0.337). Conclusion: Compared with RPV puncture, in TIPS created through the LPV, the targeted PSG was reached with a smaller stent diameter. However, no significant difference in clinical outcomes was observed.

Raw Data for the article: Brain magnetic resonance imaging radiomics features associated with hepatic encephalopathy in adult cirrhotic patients

Purpose: Hepatic encephalopathy (HE) is a potential complication of cirrhosis. Magnetic resonance imaging (MRI) may demonstrate hyperintense T1 signal in the globi pallidi. The purpose of this study was to evaluate the performance of MRI-based radiomic features for diagnosing and grading chronic HE in adult patients affected by cirrhosis. Methods: Adult patients with and without cirrhosis underwent brain MRI with identical imaging protocol on a 3T scanner. Patients without history of chronic liver disease were the control population. HE grading was based on underlying liver disease, severity of clinical manifestation, and number of encephalopathic episodes. Texture analysis was performed on axial T1-weighted images on bilateral lentiform nuclei at the level of the foramina of Monro. Diagnostic performance of texture analysis for the diagnosis and grading of HE was assessed by calculating the area under the receiver operating characteristics (AUROC) with 95% confidence interval (CI). Results: The final study population consisted of 124 patients, 70 cirrhotic patients, and 54 non-cirrhotic controls. Thirty-eight patients had history of HE with 22 having an HE grade > 1. The radiomic features predicted the presence of HE with an AUROC of 0.82 (95% CI: 0.73, 0.90; P < .0001; 82% sensitivity, 66% specificity). Radiomic features predicted grade 1 HE (AUROC 0.75; 95% CI: 0.61, 0.89; P < .0001; 94% sensitivity, 60% specificity) and grade ≥ 2 HE (AUROC 0.82; 95% CI: 0.71, 0.93; P < .0001, 95% sensitivity, 57% specificity). Conclusion: In cirrhotic patients, MR radiomic is effective in predicting the presence of chronic HE and in grading its severity.

Raw Data for the article: Clinical outcomes of transjugular intrahepatic portosystemic shunt with PTFE-covered stents after liver transplantation and technical results in split and whole liver graft recipients

Objectives: To assess the outcomes of transjugular intrahepatic portosystemic shunt (TIPS) creation using PTFE-covered stents in liver transplant (LT) recipients and to analyze the technical result of TIPS creation in split grafts (SG) compared with whole liver grafts (WG). Methods and materials: Single-center, retrospective study, analyzing LT patients who underwent TIPS using PTFE-covered stents. Clinical and technical variables were analyzed. Results: Between 2005 and 2021, TIPS was created using PTFE-covered stents in 48 LT patients at a median of 43 months (range, 0.5-192) after LT. TIPS indications were refractory ascites (RA) in 33 patients (69%), variceal bleeding (VB) in 9 patients (19%), others in 6 (12%). Ten patients (21%) received a SG. Technical success rate was 100% in both groups: in two WG recipients, (5%) a second attempt was required. An unconventional approach (combined transhepatic or transplenic access) was needed in 2 WG (5%) and 2 SG recipients (20%). Two procedure-related death occurred in the WG group. After a median follow-up of 22 months (range, 0,1-144), 16 patients (48%) in the RA group did not require post-TIPS paracentesis, in the VB group rebleeding occurred in 3 patients (33%). Fifteen patients (31%) underwent TIPS revision. Overt hepatic encephalopathy occurred in 14 patients (29%). Patient survival at 6 months, 1 year, and 3 years was 77%, 66%, and 43%, respectively. Conclusions: The feasibility and safety of TIPS creation in SG are comparable to that of WG. TIPS creation using PTFE-covered stents represents a viable option to treat portal hypertensive complications in LT recipients.

Raw Data for the article: Mortality after transjugular intrahepatic portosystemic shunt in older adult patients with cirrhosis: A validated prediction model

Background and aims: Implantation of a transjugular intrahepatic portosystemic shunt (TIPS) improves survival in patients with cirrhosis with refractory ascites and portal hypertensive bleeding. However, the indication for TIPS in older adult patients (greater than or equal to 70 years) is debated, and a specific prediction model developed in this particular setting is lacking. The aim of this study was to develop and validate a multivariable model for an accurate prediction of mortality in older adults. Approach and results: We prospectively enrolled 411 consecutive patients observed at four referral centers with de novo TIPS implantation for refractory ascites or secondary prophylaxis of variceal bleeding (derivation cohort) and an external cohort of 415 patients with similar indications for TIPS (validation cohort). Older adult patients in the two cohorts were 99 and 76, respectively. A cause-specific Cox competing risks model was used to predict liver-related mortality, with orthotopic liver transplant and death for extrahepatic causes as competing events. Age, alcoholic etiology, creatinine levels, and international normalized ratio in the overall cohort, and creatinine and sodium levels in older adults were independent risk factors for liver-related death by multivariable analysis. Conclusions: After TIPS implantation, mortality is increased by aging, but TIPS placement should not be precluded in patients older than 70 years. In older adults, creatinine and sodium levels are useful predictors for decision making. Further efforts to update the prediction model with larger sample size are warranted.

Raw Data for the article: Growth Performance and Recovery of Nosocomial Aspergillus spp. in Blood Culture Bottles

Theoretically, Aspergillus spp. grow in culture media, but frequently, blood cultures of patients with invasive Aspergillosis are negative, even if until now, the reasons are not clear. This aspect underlines the lack of a good strategy for the cultivation and isolation of Aspergillus spp. In order to develop a complete analytical method to detect Aspergillus in clinical and pharmaceutical samples, we investigated the growth performance of two blood culture systems versus the pharmacopeia standard method. At &lt;72 h, all test systems showed comparable sensitivity, about 1-2 conidia. However, the subculture analysis showed a suboptimal recovery for the methods, despite the positive growth and the visualization of the "Aspergillus balls" in the culture media. To investigate this issue, we studied three different subculture approaches: (i) the use of a sterile subculture unit, (ii) the use of a sterile subculture unit and the collection of a larger aliquot (100 µL), following vigorous agitation of the vials, and (iii) to decapsulate the bottle, withdrawing and centrifuging the sample, and aliquot the pellet onto SDA plates. Our results showed that only the third procedure recovered Aspergillus from all positive culture bottles. This work confirmed that our strategy is a valid and faster method to culture and isolate Aspergillus spp. from blood culture bottles.