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132 results for “Amoebozoa”
Spreadsheet Template for Wolfgang Bettighofer Amoebozoa Trait Data
<p>Spreadsheet template for <a href="https://doi.org/10.5281/zenodo.13320925">Wolfgang Bettighofer Amoebozoa Trait Data</a></p>
Fig. 4 A–D in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 4 A–D Ultrastructure of the nucleus of Thecochaos fibrillosum. A Cross-section through the nucleus of amoeba and the contractile vacuole surrounded with tubulovesicular spongiom. B A portion of the nuclear membrane with the underlying microfilamentous layer (black arrow) and nuclear pores (black arrowheads). C The fragment of the patchy nucleolus showing several small lacunae filled with the karyoplasm (one is marked with a white asterisk). Small patches of electron-dense material, appearing darker than the nucleolus' main material, are labeled with white arrows. A granule of electron-dense material resembling chromatin is labeled with the white arrowhead. D The fragment of amoebae cell showing the nucleus and the spongiom of the contractile vacuole localized near the nucleus. This is another section of the same nucleus as illustrated in A. Abbreviations: nu, nucleolus; l, lacuna; b, bacteria; m, mitochondria; cv, contractile vacuole; pm, plasma membrane; nm, nuclear membrane; n, nucleus; s, spongiom. Scale bars: A, D, 1 μm; B, 200 nm; C, 500 nm
Fig. 3 A–E in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 3 A–E Ultrastructure of Thecochaos fibrillosum. A A fragment of the amoeba cell demonstrating the stratification of the cytoplasm into the peripheral hyaloplasm and the inner granuloplasm. Numerous vacuoles and vesicles are noticeable at the periphery of the granuloplasm. B Cell coat consisting of the loose filamentous layer (distinct filaments are pointed by black arrows), covering a thick basal layer of electron-dense amorphous glycocalyx (black double arrowheads). C The peripheral area of the hyaloplasm, containing microfilamentous bundles, perpendicular to the plasma membrane (black arrowheads). Black arrows indicate cross-sections of filaments belonging to the upper layer of the glycocalyx. D An area of the granuloplasm containing dictyosomes of the Golgi complex, mitochondria with tubular cristae and dense matrix, and a single-membraned microbody. E Vacuoles containing bacteria. Abbreviations: pm, plasma membrane; hl, hyaloplasm; gr, granuloplasm; m, mitochondria; d, dictyosome of the Golgi complex; b, bacteria. Scale bars: A, 1 μm; B and C, 200 nm; D and E, 500 nm
Fig. 7 A–I in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 7 A–I Materials on Thecochaos species hold in the collection of E. Penard's slides deposited with the British Museum of Natural History (London). DIC optics is used to enhance the contrast of these rather bleach ancient preparations. A Page from the collection catalog, listing seven slides of T. album and the only slide of T. fibrillosum. B, C Actual view of these slides, showing the high quality of mount and good conditions of the preparation. D, E Thecochaos album, the overall appearance of the cell. F Thecochaos album nuclei showing the peripheral position of the nucleolar material
Fig. 2 A–M in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 2 A–M Light microscopy of Thecochaos fibrillosum, DIC. A–C Moving cells on the glass surface. D–M Light microscopy of the cytoplasm and nuclei of Thecochaos fibrillosum, showing variants of
Fig. 6 A, B in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 6 A, B Multigene phylogeny of Thecochaos fibrillosum. A Tree based on the alignment of 58 genes, each individual gene alignment contained both Thecochaos (11 021 AA positions in total) and Stratorugosa gene sequences (18 761 AA positions in total). RAxML (LGX+ G4m model + I)/ Phylobayes (LG model with CAT approximation)/IqTree/ (LG+ F + R4 model). Black circles indicate full support by all methods used. "–" indicates that the pattern of branching was not recovered by one of the reconstruction methods used. Gray arrow indicates the alterative position of Stratorugosa clade under PhyloBayes analysis. B Tree based on the alignment of 151 protein-coding genes including either Thecochaos (13 898 AA positions) or Stratorugosa sequences (42 744 AA positions) or both, so the 58 gene set is also in. IQTree; Sh-aLRT supports values are indicated
Fig. 8 A–E in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 8 A–E Schemes or phylogenetic trees of Discosea obtained in SSU and multigene phylogenetic analyses and comparison of two alternative configurations obtained in the present SSU phylogenetic analysis
Fig. 5 in Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) - a rediscovered group of lobose amoeba, with short SSU gene
Fig. 5 SSU phylogenetic tree showing the position of Thecochaos fibrillosum. PhyML analysis (GTRGAMMA model and a proportion of invariant sites; 1620 sites used) and Bayesian analysis (GTR- GAMMA model (8 rate categories) and the covarion model, same mask). Black circles indicate full support (100/1.0 of BS/PP). Open
FIGURE 4 in New records of Myxomycetes (Amoebozoa) from the tropical Andes
FIGURE 4. New records of Myxomycetes contributed in this work. (○) Previous distribution of the species, (●) new records.
FIGURE 4 in Meisterfeldia bitsevi-new testate amoeba of the family Cryptodifflugiidae Jung 1942 (Amoebozoa: Arcellinida) from the tree hollow in the urban park (Moscow Russia) with a key to species of the genus Meisterfeldia
FIGURE 4. Comparison of mean shell size of different species from the genus Meisterfeldia.
Fig. 4. Certesella larai n in Certesella larai (Amoebozoa: Arcellinida: Hyalospheniformes) a new soil testate amoeba species from the Dominican Republic and Chile challenges the definition of genera Certesella and Porosia
Fig. 4. Certesella larai n.sp. scanning electron microscopy images showing the overall shape, the chitinous rim of the pseudostome, the detail of an elongated pore and the plates (presumably recycled from euglyphid testate amoebae) covering the shell.
Fig. 1 in Electron Microscopical Investigations of a New Species of the Genus Sappinia (Thecamoebidae, Amoebozoa), Sappinia platani sp. nov., Reveal a Dictyosome in this Genus
Fig. 1. Phylogenetic tree of the genus Sappinia splitted into five species based on SSU rRNA gene sequence data. Sappinia platani sp. nov. described in this study is highlighted in bold. The tree is inferred from Bayesian analysis using Thecamoeba quadrilineata (DQ122381) as outgroup taxon (not shown). Posterior probabilities are indicated at the nodes. Corresponding bootstrap values for a maximum likelihood analysis are shown (Bayes/ML). GenBank accession numbers for SSU rRNA gene sequences are indicated in parentheses.
FI GU R E 1 Schematic drawing of Apodera angatakere and codes of the measurements taken. (A) Length of the test; (B) width of test; (C) width of the pseudostome; (D) length of the neck; (E) maximal width of the neck; (F) width of the test at the constriction; (G) length of the test without the neck in Superficially described and ignored for 92 years, rediscovered and emended: Apodera angatakere (Amoebozoa: Arcellinida: Hyalospheniformes) is a new flagship testate amoeba taxon from Aotearoa (New Zealand)
FI GU R E 1 Schematic drawing of Apodera angatakere and codes of the measurements taken. (A) Length of the test; (B) width of test; (C) width of the pseudostome; (D) length of the neck; (E) maximal width of the neck; (F) width of the test at the constriction; (G) length of the test without the neck
Fig. 5 in Neotypification of Difflugia biwae (Amoebozoa: Tubulinea: Arcellinida) from the Lake Biwa, Japan
Fig. 5. Scanning electron micrographs of the shell of Difflugia biwae. A, Lateral view of whole shell; B, apertural view showing the circular aperture is surrounded by a conspicuous great collar flare; C, lateral-apertural view of collar flare; D, composed of mineral particles or diatom frustules (typically Praestephanos suzukii, endemic diatom of Lake Biwa) as xenosomes, captured from their environment; E, detail of surface of collar flare rim (inside) with small angular particles and flattish pieces of quartz; F, detail of surface of collar flare rim (outside); G, tip of the protuberance with flattish pieces of quartz. Sampling data of these specimens was given as Morph#1 in the Materials and Methods section. Scales are given in each photo.
Fig. 3 in Molecular Phylogenetics Evidence for a Novel Lineage of Amoebae Within Discosea (Amoebozoa: Lobosa)
Fig. 3. Maximum-Likelihood trees including partial sequences retrieved from GenBank, based on the 660-bp Ami fragment (A – LG30-03 positions: 566-1228), and on the first 5' 550-bp portion (B – LG30-03 positions: 1-547). Trees were rooted on the Dermamoebidae. At nodes, BV for ML/NJ/MP (filled circles – 100% BV with all methods; * – node supported but BV <40%). See Fig. 1 for acc. nos.
Fig. 8 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa
Fig. 8. Fine structure of the cyst wall. A – an early stage of cyst wall development shows the electron dense inner layer (1) and closely apposed finely fibrillar outer layer (2) surrounded by a mucoid thicker layer (3); B – a more mature cyst wall with undulating electron dense inner layer (1), thickened fibrous outer layer (2) consisting of a proximal finely fibrillar layer and a more distal denser layer, surrounded by a much thickened mucoid layer (3) containing organic debris particles and occasional bacteria (B). Scale bars in A, B: 1 µm.
Fig. 6 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa
Fig. 6. Phase contrast micrographs of T. tubasferens cysts. A – typical round, thin-walled cysts; B – typical irregular cysts, walls are even thinner than in spherical cysts; C – thick-walled, spherical cysts, note that besides the wall being thicker, there is a space between the cell and the cyst wall; D, E – an irregular shaped cyst from which the amoeba excysted successfully; F, G – a typical combination of cysts that come from a single amoeba, one irregular cyst surrounded by two thin or thick walled cysts (1 indicates an irregular cyst, 2 indicates the inner part of a thick walled cyst and 3 indicates the wall); H, I and J – amoebae exiting their cysts, cysts are indicated by the white arrow, amoebae are indicated by the black arrow. Scale bar for all images: 20 µm.
Fig. 5 in Morphological Description of Telaepolella tubasferens n. g. n. sp., Isolate ATCC© 50593™, a Filose Amoeba in the Gracilipodida, Amoebozoa
Fig. 5. Phase contrast micrographs of floating form of T. tubasferens. A – immediately before floating; B, C, D – actively floating. In C, there are three separate floaters, which united in the water column, apparently the cytoplasm has not joined. In D there are two separate floating forms. E–I – phase contrast micrographs of a floating T. tubasferens purposely flattened for observation. These are sequential images of the initial moments after forceful flattening. The amoeba clearly shows lobose pseudopods (all images) and the nuclei gathered at the center, especially visible in I. Scale bar for all images: 20 µm.
Plate 2 in Diversity of testate amoebae (Amoebozoa and Cercozoa) in Western Ghats of Kerala with 8 new records to India
Plate 2. New records of India. (A) Argynnia gertrudeana Jung, 1942, (B) Euglypha marginata Van Oye, 1958, (C) Euglypha strigosa heterospina Wailes, 1912, (D) Physochilla tenella Penard, 1893.
Data from: Genetic barcoding of dark-spored myxomycetes (Amoebozoa)—Identification, evaluation and application of a sequence similarity threshold for species differentiation in NGS studies
Unicellular, eukaryotic organisms (protists) play a key role in soil food webs as major predators of microorganisms. However, due to the polyphyletic nature of protists, no single universal barcode can be established for this group, and the structure of many protistean communities remains unresolved. Plasmodial slime moulds (Myxogastria or Myxomycetes) stand out among protists by their formation of fruit bodies, which allow for a morphological species concept. By Sanger sequencing of a large collection of morphospecies, this study presents the largest database to date of dark-spored myxomycetes and evaluate a partial 18S SSU gene marker for species annotation. We identify and discuss the use of an intraspecific sequence similarity threshold of 99.1% for species differentiation (OTU picking) in environmental PCR studies (ePCR) and estimate a hidden diversity of putative species, exceeding those of described morphospecies by 99%. When applying the identified threshold to an ePCR data set (including sequences from both NGS and cloning), we find 64 OTUs of which 21.9% had a direct match (>99.1% similarity) to the database and the remaining had on average 90.2 ± 0.8% similarity to their best match, thus thought to represent undiscovered diversity of dark-spored myxomycetes.
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