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FIGURE 5 in Revision of the horseface loaches (Cobitidae, Acantopsis), with descriptions of three new species from Southeast Asia

FIGURE 5. (A) Dorsal VieW of erectile, bifid 'suborbital' spine (highlighted) on snout of A. rungthipae, UF 188112, 70.0 mm SL. Pectoral fins of A. dinema, (B) UF 185131, 90.3 mm SL male, and (C) UF 188116, paratype, 108.9 mm SL female; and A. rungthipae (D) UF 235976, paratype, 94.5 mm SL male, and (E) THNHM F0013610, holotype, 107.4 mm SL female.

FIGURE 3 in Revision of the horseface loaches (Cobitidae, Acantopsis), with descriptions of three new species from Southeast Asia

FIGURE 3. Snout length/head length relatiVe to standard length in (A) Acantopsis dialuzona (Y = 0.141X + 51.887; r2 = 0.813), and (B) A. rungthipae (Y = 0.136X + 49.056, r2 = 0.722). Dots represent specimens measured. RMNH 2707 is the Value for the syntype of Acantopsis dialuzona, and RMNH 4977 is the Value for the lectotype of Cobitis choirorhynchos.

FIGURE 2. Principal components analysis. Size accounted for 90.1 in Revision of the horseface loaches (Cobitidae, Acantopsis), with descriptions of three new species from Southeast Asia

FIGURE 2. Principal components analysis. Size accounted for 90.1% of the obserVed Variance. The sheared second and third principal components (PC2 and PC3) accounted for 3.7% and 2.3% of the obserVed Variance, respectiVely. Body depth (0.46) and caudal-peduncle length (-0.43) had the highest loadings on sheared PC2. Pectoral-fin length (-0.49) had the highest loading on sheared PC3.

FIGURE 1 in Revision of the horseface loaches (Cobitidae, Acantopsis), with descriptions of three new species from Southeast Asia

FIGURE 1. Mouths of Acantopsis. (A) A. thiemmedhi, UF 188118, 107.5 mm SL, With one pair of labial barbels. (B) A. rungthipae, THNHM F0013610, holotype, 107.4 mm SL (ex. UF 235976), Without labial barbels. (C) Acantopsis dialuzona, UF 173552, 114.3 mm SL, With 2 pairs of labial barbels (usually 3 in A. dialuzona). Note barbels are at least tWice as long as longest papilla. LB, labial barbel; MB, mandibular barbel; P, papilla; RB, rostral barbel; XB, maxillary barbel.

FIGURE 4 in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 4. Holotype of Rhinogobius flumineus (Mizuno 1960), TKPM-P 3000, 37.0 mm SL. Lateral (upper) and dorsal (lower) views.

FIGURE 7. Soft X in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 7. Soft X-ray negatives of holotype and five paratypes of Rhinogobius flumineus (Mizuno 1960). (A) TKPM-P 3000, holotype, 37.0 mm SL. (B) TKPM-P 3001, paratype, 34.2 mm SL. (C) TKPM-P 3002, paratype, 33.5 mm SL. (D) TKPM-P 3003, paratype, 32.3 mm SL. (E) TKPM-P 3004, paratype, 38.2 mm SL. (F) TKPM-P 3005, paratype, 34.3 mm SL.

FIGURE 1 in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 1. Holotype of Cobitis takatsuensis Mizuno 1970, TKPM-P 3006, 53.4 mm SL. Lateral (upper) and dorsal (lower) views.

FIGURE 6 in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 6. Cephalic sensory system of Rhinogobius flumineus (Mizuno 1960), TKPM-P 3000, holotype, 37.0 mm SL. Dorsal (upper), lateral (middle), and ventral (lower) views. Sensory canal pores [B', C, D (S), E, F', H', K', L', M', N, and O'] and sensory papillae (small dots) are shown. AN, anterior nasal pore; PN, posterior nasal pore. Scale bar represents 1 mm.

FIGURE 3. Soft X in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 3. Soft X-ray negatives of holotype and two paratypes of Cobitis takatsuensis Mizuno 1970. (A) TKPM-P 3006, holotype, 53.4 mm SL. (B) TKPM-P 3007, paratype, 70.0 mm SL. (C) TKPM-P 3008, paratype, 53.1 mm SL.

FIGURE 2 in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 2. Paratypes of Cobitis takatsuensis Mizuno 1970. (A) TKPM-P 3007, 70.0 mm SL. (B) TKPM-P 3008, 53.1 mm SL.

FIGURE 5 in Notes on type specimens of Rhinogobius flumineus (Mizuno 1960) (Perciformes: Gobiidae) and Cobitis takatsuensis Mizuno 1970 (Cypriniformes: Cobitidae) in Tokushima Prefectural Museum, Tokushima, Japan

FIGURE 5. Paratypes of Rhinogobius flumineus (Mizuno 1960). (A) TKPM-P 3001, 34.2 mm SL. (B) TKPM-P 3002, 33.5 mm SL. (C) TKPM-P 3003, 32.3 mm SL. (D) TKPM-P 3004, 38.2 mm SL. (E) TKPM-P 3005, 34.3 mm SL.

FIGURE 7 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 7. Cobitis feroniae, from top, Italy: not preserved, female, 65 mm SL; not preserved, male, about 35 mm SL; Sarno at Nocera Inferiore; FSJF 4123, male, 53 mm SL; spring Fiumetta at Terracina.

FIGURE 1 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 1. Maximum likelihood estimation of the phylogenetic relationships of Cobitis species included in the Adriatic group by Perdices et al. (2008) and Buj et al. (2014). based on the mitochondrial COI barcode region (HKY model, discrete Gamma distribution for rate differences with 3 categories +G parameter = 0.1065). Nucleotide positions with less than 98% site coverage were eliminated, resulting in 450 analysed positions. Numbers of major nodes indicate bootstrap values from 1000 pseudoreplicates from the ML, NJ and MP method. Dashed bars right to the specimen labels indicate species demarcation results from the ASAP partitions. The tree is drawn to scale (except to the outgroup) with branch lengths depicting number of substitutions per site.

FIGURE 4 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 4. Cobitis feroniae, ZFM-ICH-133406–ICH-133409, paratypes, from top: male, 45 mm SL; male, 47 mm SL; female, 65 mm SL; female, 69 mm SL; Italy: Fosso del Moscarello at Latina.

FIGURE 6 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 6. Cobitis feroniae, ZFMK-ICH-133406–ICH-133409, paratypes, from top: female, 69 mm SL; female, 65 mm SL; male, 45 mm SL; male, 47 mm SL; Italy: Fosso del Moscarello at Latina, right side reversed.

FIGURE 3 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 3. Cobitis feroniae, ZFMK-ICH-133324, holotype, 47 mm SL; Italy: Fosso del Moscarello at Latina.

FIGURE 2 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 2. Records of Cobitis feroniae and C. zanandreai. Data based on personal collections and previous records (in literature) published by Cavicchioli (1965), Bruno (1983), and Maio (2012).

FIGURE 8 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 8. Cobitis zanandreai, from top, Italy: ZFMK-ICH-133391, female, about 70 mm SL; ZFMK-ICH-133392, male, about 40 mm SL; Melfa at Roccasecca; not preserved, female, about 70 mm SL; Sacco at Olevano Romano; not preserved, female, about 60 mm SL; Vandra at Monteroduni.

FIGURE 5 in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 5. Cobitis feroniae, ZFMK-ICH-133324, holotype, 47 mm SL; Italy: Fosso del Moscarello at Latina, right side reversed.

FIGURE 9. Putative C in Cobitis feroniae, a new spined loach from southern Latium, Italy (Teleostei: Cobitidae)

FIGURE 9. Putative C. bilineata hybrids, from top, Italy: FSJF-DNA 4042, female, about 40 mm, hybrid between C. bilineata and C. feroniae; not preserved, female, about 60 mm; not preserved, female, about 70 mm; not preserved, female, about 70 mm; Amaseno river at Prossedi.