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777 results for “GFP”
AQ2935 unc-79(e1068)III; nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-09T10:59:57+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=_5Et0uD_vfI</li> <li><b>strain</b> : AQ2935</li> <li><b>timestamp</b> : 2011-11-09T10:59:57+00:00</li> <li><b>gene</b> : nRHO-1;unc-79</li> <li><b>chromosome</b> : I;III</li> <li><b>allele</b> : nzIs29;e1068</li> <li><b>strain_description</b> : unc-79(e1068)III; nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : unc-79 (e1068) nRHO-1 QT309 on food R_2011_11_09__10_59_57___1___3</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 25648</li> </ul>
AQ2932 nca-2(gk5)III; unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-09T11:18:16+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=YU5u6V-yjxI</li> <li><b>strain</b> : AQ2932</li> <li><b>timestamp</b> : 2011-11-09T11:18:16+00:00</li> <li><b>gene</b> : nca-2;nRHO-1;unc-77</li> <li><b>chromosome</b> : III;I;IV</li> <li><b>allele</b> : gk5;nzIs29;gk9</li> <li><b>strain_description</b> : nca-2(gk5)III; unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nca-1 (gk9) nca-2 (gk5) nRHO-1 QT309 on food R_2011_11_09__11_18_16___1___4</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 23743</li> </ul>
AQ2932 nca-2(gk5)III; unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-11T12:11:09+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=ieaDoxBUe18</li> <li><b>strain</b> : AQ2932</li> <li><b>timestamp</b> : 2011-11-11T12:11:09+00:00</li> <li><b>gene</b> : nca-2;nRHO-1;unc-77</li> <li><b>chromosome</b> : III;I;IV</li> <li><b>allele</b> : gk5;nzIs29;gk9</li> <li><b>strain_description</b> : nca-2(gk5)III; unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nca-1 (gk9) nca-2 (gk5) nRHO-1 QT309 on food L_2011_11_11__12_11_09___1___1</li> <li><b>total time (s)</b> : 899.033</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 19250</li> </ul>
AQ2934 nca-2(gk5);nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-10T11:13:29+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=PEGrPFKkeQI</li> <li><b>strain</b> : AQ2934</li> <li><b>timestamp</b> : 2011-11-10T11:13:29+00:00</li> <li><b>gene</b> : nca-2;nRHO-1</li> <li><b>chromosome</b> : III;I</li> <li><b>allele</b> : gk5;nzIs29</li> <li><b>strain_description</b> : nca-2(gk5);nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nca-2 (gk5) nRHO-1 QT309 on food R_2011_11_10__11_13_29___1___4</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 5848</li> </ul>
AQ2933 unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-04T11:23:02+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=C-kQKqeRuQQ</li> <li><b>strain</b> : AQ2933</li> <li><b>timestamp</b> : 2011-11-04T11:23:02+00:00</li> <li><b>gene</b> : nRHO-1;unc-77</li> <li><b>chromosome</b> : I;IV</li> <li><b>allele</b> : nzIs29;gk9</li> <li><b>strain_description</b> : unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nca-1 (gk9) nRHO-1 QT309 on food R_2011_11_04__11_23_02___1___6</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 26717</li> </ul>
AQ2932 nca-2(gk5)III; unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-10T10:13:17+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=LxGHdUeJsb0</li> <li><b>strain</b> : AQ2932</li> <li><b>timestamp</b> : 2011-11-10T10:13:17+00:00</li> <li><b>gene</b> : nca-2;nRHO-1;unc-77</li> <li><b>chromosome</b> : III;I;IV</li> <li><b>allele</b> : gk5;nzIs29;gk9</li> <li><b>strain_description</b> : nca-2(gk5)III; unc-77(gk9)IV; nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nca-1 (gk9) nca-2 (gk5) nRHO-1 QT309 on food R_2011_11_10__10_13_17___1___1</li> <li><b>total time (s)</b> : 899.033</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 25646</li> </ul>
AQ2935 unc-79(e1068)III; nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-11-10T10:33:42+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=ixBKs3YQBjk</li> <li><b>strain</b> : AQ2935</li> <li><b>timestamp</b> : 2011-11-10T10:33:42+00:00</li> <li><b>gene</b> : nRHO-1;unc-79</li> <li><b>chromosome</b> : I;III</li> <li><b>allele</b> : nzIs29;e1068</li> <li><b>strain_description</b> : unc-79(e1068)III; nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : unc-79 (e1068) nRHO-1 QT309 on food L_2011_11_10__10_33_42___1___2</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 26336</li> </ul>
QT309 nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-10-18T15:37:22+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=UuoQ8rvfBL0</li> <li><b>strain</b> : QT309</li> <li><b>timestamp</b> : 2011-10-18T15:37:22+01:00</li> <li><b>gene</b> : nRHO-1</li> <li><b>chromosome</b> : I</li> <li><b>allele</b> : nzIs29</li> <li><b>strain_description</b> : nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nRHO-1 (QT309) on food R_2011_10_18__15_37_22___1___10</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.48944</li> <li><b>number of segmented skeletons</b> : 6747</li> </ul>
QT309 nzIs29[punc-17::rho-1(G14V); punc-122::GFP] | 2011-10-13T12:10:29+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=-LylwvY9wSM</li> <li><b>strain</b> : QT309</li> <li><b>timestamp</b> : 2011-10-13T12:10:29+01:00</li> <li><b>gene</b> : nRHO-1</li> <li><b>chromosome</b> : I</li> <li><b>allele</b> : nzIs29</li> <li><b>strain_description</b> : nzIs29[punc-17::rho-1(G14V); punc-122::GFP]</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : nRHO (QT309) on food L_2011_10_13__12_10_29___1___3</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.43247</li> <li><b>number of segmented skeletons</b> : 6080</li> </ul>
Urbanus and Cosgrove Nature Communications (2023) - scRNAseq fastq files GFP negative sample in Figure 2
<p>This dataset contains .fastq files for the GFP negative sample in the scRNAseq dataset used in Figures 2. For any questions about this dataset please contact Leila Perie (leile.perie@curie.fr)</p>
Raw microscopy data of split-GFP and immunofluorescence experiments
<p><span>Mitochondria critically rely on protein import and its tight regulation. Here, we found </span><span>that the complex I assembly factor NDUFAF8 follows a two-step import pathway </span><span>linking IMS and matrix import systems. A weak targeting sequence drives TIM23-</span><span>dependent NDUFAF8 matrix import, and</span> <span>en route</span> <span>allows exposure to the IMS </span><span>disulphide relay which oxidizes NDUFAF8. Import is closely surveyed by proteases: </span><span>YME1L prevents accumulation of excess NDUFAF8 in the IMS, while CLPP </span><span>degrades reduced NDUFAF8 in the matrix. Therefore, NDUFAF8 can only fulfil its </span><span>function in complex I biogenesis if both oxidation in the IMS and subsequent matrix </span><span>import work efficiently. We propose that the two-step import pathway for NDUFAF8 </span><span>allows to integrate the activity of matrix complex I biogenesis pathways with the </span><span>activity of the mitochondrial disulphide relay system in the IMS. Such coordination </span><span>might not be limited to NDUFAF8 as we identified further proteins that can follow </span><span>such a two-step import pathway. The raw microscopy data provided in this dataset were obtained to visualize the localization of NDUFAF8 and further proteins following a two-step import pathway.</span><span><br></span></p>
Magnetic control of GFP-like fluorescent proteins - Raw data 1
<p>We've discovered a simple, nontoxic, biocompatible way to control the brightness of <a href="https://www.fpbase.org/protein/avgfp/">GFP</a>-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. <a href="https://www.fpbase.org/protein/egfp/">EGFP</a>, <a href="https://www.fpbase.org/protein/mscarlet/">mScarlet</a>) become magnetoresponsive in the presence of an appropriate cofactor (e.g. EGFP-<a href="https://doi.org/10.1021/acs.bioconjchem.1c00306">FlavinTag</a>, or an mScarlet/<a href="https://pubchem.ncbi.nlm.nih.gov/compound/Flavin-mononucleotide">FMN</a> solution). This method works at room-temperature and body-temperature, in vitro, in <i>E. coli</i> and in cultured mammalian cells.</p><p>The GFP-family magnetoresponse is weak (ΔF/F≈1%), but shows the hallmarks of evolvability. This suggests exciting technological possibilities, both short-term (e.g. lock-in detection, multiplexing) and long-term (e.g. optically-detected MRI, magnetogenetics).</p><p>We've also discovered weak magnetoresponse from a member of the LOV-domain family. This suggests the possibility that magnetoresponse is a general feature of fluorescent proteins, and not unique to the cryptochrome/photolyase family.</p><p> </p><p>This repository holds some of the raw data for the main text figures. For the contents of the paper, please see: <a href="https://doi.org/10.5281/zenodo.8137174">doi.org/10.5281/zenodo.8137174</a></p>
Magnetic control of GFP-like fluorescent proteins - Raw data 2
<p>We've discovered a simple, nontoxic, biocompatible way to control the brightness of <a href="https://www.fpbase.org/protein/avgfp/">GFP</a>-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. <a href="https://www.fpbase.org/protein/egfp/">EGFP</a>, <a href="https://www.fpbase.org/protein/mscarlet/">mScarlet</a>) become magnetoresponsive in the presence of an appropriate cofactor (e.g. EGFP-<a href="https://doi.org/10.1021/acs.bioconjchem.1c00306">FlavinTag</a>, or an mScarlet/<a href="https://pubchem.ncbi.nlm.nih.gov/compound/Flavin-mononucleotide">FMN</a> solution). This method works at room-temperature and body-temperature, in vitro, in <i>E. coli</i> and in cultured mammalian cells.</p><p>The GFP-family magnetoresponse is weak (ΔF/F≈1%), but shows the hallmarks of evolvability. This suggests exciting technological possibilities, both short-term (e.g. lock-in detection, multiplexing) and long-term (e.g. optically-detected MRI, magnetogenetics).</p><p>We've also discovered weak magnetoresponse from a member of the LOV-domain family. This suggests the possibility that magnetoresponse is a general feature of fluorescent proteins, and not unique to the cryptochrome/photolyase family.</p><p> </p><p>This repository holds some of the raw data for the main text figures. For the contents of the paper, please see: <a href="https://doi.org/10.5281/zenodo.8137174">doi.org/10.5281/zenodo.8137174</a></p>
GFP and JPH2 fluorescence and WGA staining in JPH2+ ARVMs at day 4 in culture: views across the z-axis.
<p><strong>Supplementary Video 1. Complete views across the Z-axis (from bottom to top) for GFP (<em>left</em>), JPH2 (<em>middle</em>) and WGA (<em>right</em>) channels for two day 4 ventricular myocytes cultured with Adv-JPH2-GFP. </strong>The above cell has greater GFP (left) and JPH2 (middle) intensity, indicating more successful viral transduction, but internal WGA staining (right) is little different for both cells. T-tubules are visible in the WGA channel towards the very top and very bottom of each cell. The punctate nature of JPH2 staining in the above cell is readily apparent throughout. Z-depth was 1 µm. The non-cell staining apparent in the WGA channel for deeper Z-slices is staining of the cover slip.</p>
Raw microscopy data of split-GFP and immunofluorescence experiments
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RNA seq of 4-week-old SAB23KO mutants and wild-type plants soaked for 0 or 1 day, and 6 ChIP libraries sequence (two DJ and four SAB23-GFP)
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Confocal image stacks of GFP expression in Drosophila forelegs driven by Gal4 driver expression in foreleg motor neurons
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Data from: Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice
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Data underlying figures in "Turning on the emission from isolated GFP chromophore anions at cryogenic temperatures"
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Videos for MRC5 cells stably expressing GFP-Rab5
<p>Videos of MRC5 cells stably expressing GFP-Rab5.</p>
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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