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Figure 2 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America
Figure 2. Drawings of Trimyema foissneri from life (A–D) and aħer protargol staining (E–I). A, leħ lateral view of a representative individual, arrow shows acontractile vacuole, red arrowhead shows food vacuole, yellow arrowheads show somatic cilia. B, different body shape in different viewing angles. C, leħ lateral view, showing buccal cavity (arrow) and position of macronucleus. D, disposition of the ciliary girdles. E, oral ciliature, red dots showing kinetidal composition at the anterior part of OK1. F, lateral view of oral ciliature, showing the shorter oral kinety 3 (in red). G, macronucleus and micronucleus (arrowheads), the position of the laưer being variable (1, 2). H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrows show argentophilic lines connecting longitudinal somatic ciliary rows, arrowheads show epaulet. Abbreviations: CC, caudal cilia; Ma, macronucleus; OK, oral kinety; SK, somatic kineties; VLF, ventrolateral fragment. Scale bars: 15 μm (A, C); 10 μm (H, I).
Figure 6 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America
Figure 6. Drawings of Plagiopyla frontata from life (A–C, E, F) and after protargol staining (D, G–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, tangential section of the cortex, arrowhead marks straight extrusome, arrow indicates curved extrusome. C, extruded extrusomes with one end slightly inflated (red arrowheads). D, details of oral region, showing oral opening and buccal cavity (arrow). E, F, different cell shapes. G, dorsal view of a compressed cell, showing position of striated band, cytoproct (black arrow), dense ciliary rows (red arrow), and contractile vacuole pores (red arrowheads). H, ventral view to show somatic and oral kineties, arrowhead indicates gap between upper oral lip kineties and somatic kineties. I, dorsal view to show somatic kineties, arrow indicates striated band, arrowhead shows dense ciliary rows, double-arrowhead shows several irregularly and densely packed kinetosomes located near loop of striated band. Abbreviations: CV, contractile vacuole; Ma, macronucleus; SB, striated band. Scale bars: 35 μm (A, E, G, H); 10 μm (C).
Figure 7 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America
Figure 7. Photomicrographs of Plagiopyla flontata in vivo (A–H) and aħer protargol staining (I–M). A, ventral view of a slightly compressed cell, arrow marks buccal cavity. B, representative individual, arrow shows oral opening, arrowhead indicates contractile vacuole. C, extruded extrusomes with one end slightly inflated (arrowheads). D, contractile vacuole pores (red arrowheads). E, ventral view of a compressed cell, arrows show straight extrusomes, arrowheads show curved extrusomes. F, details of dorsal side, arrowhead shows cytoproct. G, right side of a compressed cell showing striated band (arrowhead). H, details of extrusomes. I, macronucleus and micronucleus (white arrowhead). J, K, oral region, showing buccal cavity under different focal planes. L, M, ventral (L) and dorsal (M) views of the same individual, showing somatic and oral kineties, red arrow indicates gap between upper oral lip kineties and somatic kineties, black arrow shows striated band, black arrowhead shows dense ciliary rows, red arrowhead indicates several polykineties located near loop of striated band. Abbreviation: Ma, macronucleus. Scale bars: 35 μm.
Figure 3 in Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America
Figure 3. Photomicrographs of Trimyema foissneri from life (A–C, J–M) and aħer protargol staining (D–I, N–º). A, B, right lateral views of different individuals, showing different body shape. C, right lateral views of a slightly compressed individual, arrowhead marks caudal cilia. D, macronucleus and micronucleus (arrowhead). E, cytopharynx fibres (arrowhead). F, lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead). G–I, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads). J, ventral view, arrowhead indicates oval buccal cavity. K, anterior region, showing buccal cavity (arrowhead). L, lateral view of cell, showing three oblique somatic ciliary girdles. M, anterior part of cell, arrowheads show dents on surface. N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P, º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (red circle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts. Abbreviations: G1, first ciliary girdle; G2, second ciliary girdle; G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment. Scale bars: 15 μm (A–C); 10 μm (G, P, º).
Data from: Temporal and spatial comparisons of angiosperm diversity between eastern Asia and North America
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Data from: Physconia labrata, a new species from western North America and Asia
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Data from: Effects of climate and topography on the diversity anomaly of plants disjunctly distributed in eastern Asia and eastern North America
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Data from: Allopatric speciation in Asia contributed to the diversity anomaly between eastern Asia and eastern North America: evidence from anchored phylogenomics of Stewartia (Theaceae)
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Figure 5 from: Sabbatini Peverieri G, Mitroiu M-D, Bon M-C, Balusu R, Benvenuto L, Bernardinelli I, Fadamiro H, Falagiarda M, Fusu L, Grove E, Haye T, Hoelmer K, Lemke E, Malossini G, Marianelli L, Moore MR, Pozzebon A, Roversi P-F, Scaccini D, Shrewsbury P, Tillman G, Tirello P, Waterworth R, Talamas EJ (2019) Surveys of stink bug egg parasitism in Asia, Europe and North America, morphological taxonomy, and molecular analysis reveal the Holarctic distribution of Acroclisoides sinicus (Huang & Liao) (Hymenoptera, Pteromalidae). Journal of Hymenoptera Research 74: 123-151. https://doi.org/10.3897/jhr.74.46701
Figure 5 Boxplot of pair-wise molecular distances between (left) the Acroclisoides haplotypes evidenced in this study, (center) conspecific individuals in the Pteromalinae, (right) individuals from different species of the same genus in the Pteromalinae.
Figure 4 from: Sabbatini Peverieri G, Mitroiu M-D, Bon M-C, Balusu R, Benvenuto L, Bernardinelli I, Fadamiro H, Falagiarda M, Fusu L, Grove E, Haye T, Hoelmer K, Lemke E, Malossini G, Marianelli L, Moore MR, Pozzebon A, Roversi P-F, Scaccini D, Shrewsbury P, Tillman G, Tirello P, Waterworth R, Talamas EJ (2019) Surveys of stink bug egg parasitism in Asia, Europe and North America, morphological taxonomy, and molecular analysis reveal the Holarctic distribution of Acroclisoides sinicus (Huang & Liao) (Hymenoptera, Pteromalidae). Journal of Hymenoptera Research 74: 123-151. https://doi.org/10.3897/jhr.74.46701
Figure 4 Haplotype network of the 49 Acroclisoides sinicus barcodes analyzed in this study. Each circle corresponds to one haplotype; circle size gives the proportion of individuals belonging to the haplotype. The color of the circles represents the geographical origin. Numbers correspond to the haplotype numbers. Hash marks symbolize the number of mutations between haplotypes.
Figure 1 from: Sabbatini Peverieri G, Mitroiu M-D, Bon M-C, Balusu R, Benvenuto L, Bernardinelli I, Fadamiro H, Falagiarda M, Fusu L, Grove E, Haye T, Hoelmer K, Lemke E, Malossini G, Marianelli L, Moore MR, Pozzebon A, Roversi P-F, Scaccini D, Shrewsbury P, Tillman G, Tirello P, Waterworth R, Talamas EJ (2019) Surveys of stink bug egg parasitism in Asia, Europe and North America, morphological taxonomy, and molecular analysis reveal the Holarctic distribution of Acroclisoides sinicus (Huang & Liao) (Hymenoptera, Pteromalidae). Journal of Hymenoptera Research 74: 123-151. https://doi.org/10.3897/jhr.74.46701
Figure 1 Typical shapes of exit holes produced by egg parasitoids of Halyomorpha halys in Europe: Anastatus bifasciatus male (A) and female (B) (NE-Italy); Acroclisoides sinicus (C, D) (NE-Italy); Trissolcus mitsukurii (E) (NE-Italy) and Trissolcus japonicus (F) (Switzerland).
Figure 3 from: Sabbatini Peverieri G, Mitroiu M-D, Bon M-C, Balusu R, Benvenuto L, Bernardinelli I, Fadamiro H, Falagiarda M, Fusu L, Grove E, Haye T, Hoelmer K, Lemke E, Malossini G, Marianelli L, Moore MR, Pozzebon A, Roversi P-F, Scaccini D, Shrewsbury P, Tillman G, Tirello P, Waterworth R, Talamas EJ (2019) Surveys of stink bug egg parasitism in Asia, Europe and North America, morphological taxonomy, and molecular analysis reveal the Holarctic distribution of Acroclisoides sinicus (Huang & Liao) (Hymenoptera, Pteromalidae). Journal of Hymenoptera Research 74: 123-151. https://doi.org/10.3897/jhr.74.46701
Figure 3 Acroclisoides sinicus: paratype of A. solus ♀ (U.S.A.), habitus in lateral view (A); idem, head in frontal view (B); ♂ (Switzerland), habitus in lateral view (C); idem, head in frontal view (D).
Figure 2 from: Sabbatini Peverieri G, Mitroiu M-D, Bon M-C, Balusu R, Benvenuto L, Bernardinelli I, Fadamiro H, Falagiarda M, Fusu L, Grove E, Haye T, Hoelmer K, Lemke E, Malossini G, Marianelli L, Moore MR, Pozzebon A, Roversi P-F, Scaccini D, Shrewsbury P, Tillman G, Tirello P, Waterworth R, Talamas EJ (2019) Surveys of stink bug egg parasitism in Asia, Europe and North America, morphological taxonomy, and molecular analysis reveal the Holarctic distribution of Acroclisoides sinicus (Huang & Liao) (Hymenoptera, Pteromalidae). Journal of Hymenoptera Research 74: 123-151. https://doi.org/10.3897/jhr.74.46701
Figure 2 Acroclisoides sinicus, ♀ (Italy, Cordenons): habitus in dorso-lateral view (A); head in frontal view (B); head in dorsal view (C); fore wing (D); antennae (E); mesosoma in dorsal view (F); mesosoma in lateral view (G).
Supplementary material 1 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
: Data type: measurement.
Figure 6 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
Figure 6 Morphological differences amongst A–DEranthis tanhoensisE–HEranthis sibirica; and I–LEranthis stellataA, E, I flower position B, F, J flowers C, G, K involucral bracts and follicles D, H, L basal leaves.
Figure 3 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
Figure 3 Mitotic metaphase chromosomes. AEranthis sibirica (voucher 1 in Table 1), 2n = 28 BEranthis sibirica (voucher 2), 2n = 28 CEranthis sibirica (voucher 3), 2n = 42 DEranthis tanhoensis (voucher 11), 2n = 14 EEranthis tanhoensis (voucher 9), 2n = 14 + 0–8 B (arrows point at B chromosomes) FEranthis stellata (voucher 14), 2n = 16. Scale bars: 10 µm.
Figure 2 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
Figure 2 Scatter point diagram in the space of the first two main components for Eranthis sibirica (red dots), Eranthis tanhoensis (green triangles) and Eranthis stellata (blue squares) A at flowering and B at fruiting stages. Ellipses enclose the regions of the space that contain each of the plant species with a 95% probability (95% confidence ellipses).
Figure 10 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
Figure 10 General distribution of Eranthis sibirica (dots) and E. tanhoensis (stars), based on herbarium materials.
Figure 1 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
Figure 1 ML tree inferred from the combined cpDNA and ITS data. The numbers above branches are bootstrap values (BS > 50%) and numbers under branches are Bayesian posterior probabilities (PP > 0.50).
Figure 4 from: Erst AS, Sukhorukov AP, Mitrenina EYu, Skaptsov MV, Kostikova VA, Chernisheva OA, Troshkina V, Kushunina M, Krivenko DA, Ikeda H, Xiang K, Wang W (2020) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 140: 75-100. https://doi.org/10.3897/phytokeys.140.49048
Figure 4 HPLC chromatograms of 70% water-ethanol extracts of Eranthis leaves detected by HPLC-DAD at 255 nm. The X-axis displays the retention time, min; Y-axis – the detector signal in optical density units. The identified peaks are 1. chlorogenic acid, 2. gentisic acid, 3. caffeic acid, 5. orientin, 8. vitexin, 10. hyperoside, 11. salicylic acid, 19. quercetin and 24. kaempferol.
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