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opencc-by-4.0Apr 2019View details →

Metabolic effects of a high level of dietary zinc in piglet diets to induce immune competence

There is increasing consumer pressure to reduce the use of therapeutic antibiotics in animal production. Solutions to this challenge require a multi-targeted approach, including use of particular functional ingredients, such as pre- and probiotics that are not only used to boost the growth and productivity but also to influence the development of immune competence and support responsiveness of the immune system at later age. In this context there is a large interest in the development and evaluation of nutritional interventions during early life stages in farm animal species. However, apart from effects on animal growth and productivity, there is lack of knowledge in livestock on ways to modify development of immune competence by nutritional manipulation. Thus an associated project is being conducted with the objective to evaluate effects of a “model intervention” in piglets to modify development of immune competence. The associated project investigates the effects of provision of diet with a high concentration of zinc (2790 mg/kg) over different time periods in post-weaned piglets as a “model intervention” for manipulation of immune competence, compared to a reference treatment, in which the animals are fed a diet with a basal level of zinc. The objective of the associated project is to investigate the effects of high concentration of zinc on functional and metabolic changes, including processes related to digestive functions of the gut, its integrity and barrier function, and on immunological processes as effected by the nutritional manipulation. In the associated project we already studied the effects of high concentration of zinc on growth performance, on composition and diversity of the microbiota in the gut, on genome-wide gene expression in intestinal tissue, and on several immune markers in blood. In this proposal we apply for funds to provide us with expertise and access to facilities in order to get a global view of the metabolic changes as induced by the high concentration of zinc by analyzing a range of metabolites in blood.

opencc-by-4.0May 2019View details →

Dataset to reproduce MD simulations described in "An atomistic view of the YiiP structural changes upon zinc(II) binding"

Here are collected topologies, parameters, distance restraints, starting coordinates and trajectories for all the simulations reported in the manuscript entitled "An atomistic view of the YiiP structural changes upon zinc(II) binding"

opencc-by-4.0Sep 2019View details →

CLPO and AIM results for copper, zinc and cadmium xanthate complexes

Results of NPA and CLPO analysis obtained by JANPA software for copper, zinc and cadmium xanthate complexes. Results of AIM obtained by MultiWFN software for copper, zinc and cadmium complexes Molecular structure in XYZ format for the complexes

opencc-by-4.0Nov 2022View details →

Datasets - Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems

Datasets for the toxicological data generated and presented in the following article: Toxicological effects of zinc oxide nanoparticle exposure: an in vitro comparison between dry aerosol air-liquid interface and submerged exposure systems. DOI: 10.1080/17435390.2021.1884301.

Growth of Zinc Crystals in eGaIn Wetted to a Zinc Surface

Zinc crystals (dark phase) growing in liquid eutectic gallium indium (right half of the video) wetted to a zinc specimen. On the left side of the video, grains in the zinc specimen can be seen changing shape.

Grain Boundary Motion in eGaIn-Zinc Embrittlement

Timelapse of grain boundary motion in a region of a zinc specimen in which the grain boundaries have been wetted by eutectic gallium indium (eGaIn). The dark gray phase is the solid zinc crystals, bright phase is indium precipitates formed by indium being rejected from the liquid phase, and the intermediate phase is molten eGaIn filling the prior grain boundaries.

Timelapse of eGaIn Penetrating Coarse Grained Zinc

Scanning electron microscope timelapse of liquid eutectic gallium indium penetrating the grain boundaries of a coarse grained zinc specimen. The dark gray phase is the solid zinc and the brightest white phase is solid indium precipitates that form as indium is displaced from the liquid phase by zinc. The intermediate gray is the liquid phase widening in the grain boundaries in the wake of the penetration front. The rate of the front was determined by tracking the first appearance of indium precipitates which was the first indication of eGaIn presence in boundaries at this scale. Liquid metal induced recrystallization is apparent in the wake of the front on the left edge of the frame.

EBSD Overlay on eGaIn-Zinc Embrittlement

Electron backscatter diffraction (EBSD) inverse pole figure (IPF) map of the microstructure of a coarse grained zinc specimen rolled to 25% roll reduction, overlaid over the same region being attacked by liquid eutectic gallium indium (eGaIn). The eGaIn (bright phase) can be seen highlighting the grain boundaries, especially in areas with a finer initial grain size. There is a period of 5 minutes between each frame. IPF colored with respect to out-of-plane direction.

Timelapse of eGaIn Penetrating Fine Grained Zinc

Scanning electron microscope timelapse of liquid eutectic gallium indium penetrating the grain boundaries of a fine grained zinc specimen. The dark gray phase is the solid zinc and the brightest white phase is solid indium precipitates that form as indium is displaced from the liquid phase by zinc. The intermediate gray is the liquid phase widening in the grain boundaries in the wake of the penetration front. The rate of the front was determined by tracking the first appearance of indium precipitates which was the first indication of eGaIn presence in boundaries at this scale.

opencc-by-4.0Apr 2023View details →

Zinc(II) bis((1R,2R)-1,2-diaminocyclohexane) dinitrate: Crystallographic raws

Crystallographic raw files of non-centrosymmetric P21 and centrosymmeric P21/n phases of the metal-organics. Consider: J. Mol. Struct. 1248 (2022) 131488 [https://www.sciencedirect.com/science/article/abs/pii/S0022286021016161] and data on Cambridge Crystallographic Data Center CCDC 1576255, 2256806, and 2256980, respectively. Results from ADDSYM test, considering symmetry elements are presented, as well.

Fig. 6 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 6. Longevity of AmSOD1 activity. Purified His-AmSOD1 was tested for its longevity both at room temperature and 4 ◦ C for different time period (20, 40, 60, 80, 100, 120 1st 140 days). The ratio enzyme activity was determined based on the activity of enzyme before incubation. Each data point represents the mean ± SD obtained from three independent reactions.

Fig. 7 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 7. Growth curves of the strains control and R-AmSOD1 in the medium without treatment and the medium containing containing (A) salt and (B) hydrogen peroxide. Each data point represents the mean ± SD obtained from three independent reactions.

Fig. 2 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 2. The effects of salt and hydrogen peroxide stress on the appearance pattern and activity of AmSOD1 in the leaves of A. marina (A) Appearance pattern of AmSOD1 and (B) AmSOD assay in the leaves responding to hydrogen peroxide and salt stress as well as control at various time points (0–48 h). Each histogram represents the mean obtained from three independent reactions (± SD). Different letters above the bars indicate statistically significant differences by LSD test.

Fig. 1 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 1. Phylogenetic tree of SOD sequences from different plants. For accession numbers and organisms, see ''Materials and Methods.''.

Fig. 4 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 4. Molecular mass of purified AmSOD1 using gel filtration. (A) Native molecular mass of recombinant AmSOD1 was measured with respect to standard protein marker as resolved in Biosil-250 analytical gel filtration column. Standard proteins used were: (a) 66.5 kDa-BSA fraction (b) 29 kDa-Carbonic anhydrase and (c) 16.7 kDa human myoglubin.

Fig. 3 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 3. SDS-PAGE analysis and the solubility of expressed His-AmSOD1 in the strain R-AmSOD1 harboring pET28a-AmSOD1 (A) Total soluble protein extracted from control strain and the strain R-AmSOD1 before and 1–4 h after induction with IPTG. (B) The study of solubility of His-AmSOD1 by comparison of His-AmSOD1 band intensities in the total protein (T), the soluble fraction extracted from R-AmSOD1 (S) and the unsoluble fraction (U) extracted from RAmSOD1 (The recombinant proteins were shown with arrows) (C) SDS-PAGE analysis of AmSOD1 after purification with affinity chromatography.

Fig. 5 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 5. Effects of incubation at different temperatures and pH on the activity of AmSOD1. The purified AmSOD1 was incubated (A) at temperatures (25, 37, 50, 60 and 75 ◦ C) (B) in buffers with pH (3–12) for 1 h before SOD assay. Then the activity of AmSOD1 was determined as explained in the "Materials and Methods". Each data point represents the mean ± SD obtained from three independent reactions.

opennotspecifiedNov 2021View details →

Fig. 2 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 2. Classification of gene expression changes observed in both the HMA4 double-knockout mutants (cv. #3) and HMA4 RNAi plants (cv.#1) relative to their respective controls. The genes were classified manually according to the putative function of the closest Arabidopsis Blast hit (see Supplementary Table S2).

opennotspecifiedNov 2021View details →

Fig. 1 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 1. (a) Relative element contents of HMA4-impaired plants compared with their controls. HMA4 RNAi plants in cv.#1 background were compared with their WT controls (n = 3 replicates each), and HMA4 double-mutant plants in cv. #3 background were compared with their null-segregant controls (n = 4). The elements were measured in dry weight of lower leaves of plants grown under greenhouse conditions. The error bars depict the 0.95 confidence interval (CI) corrected according to Satterthwaite approximation. (b) Pictures of representative plants of each group (sticker size = 11.9 cm).