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4,683 results for “embryonic”
Fig. 2 in Peculiarities Of Embryonic And Post-Embryonic Development Of Oesophagostomum Dentatum (Nematoda, Strongylidae) Larvae Cultured In Vitro
Fig. 2. Post-embryonic development of Оeso-
Fig. 1 in Peculiarities Of Embryonic And Post-Embryonic Development Of Oesophagostomum Dentatum (Nematoda, Strongylidae) Larvae Cultured In Vitro
Fig. 1. Embryonic development of Оesophagostomum dentatum in vitro: а — fragmentation of
Effects of semi-constant temperature on embryonic and hatchling phenotypes of six-tubercled Amazon River turtles, Podocnemis sextuberculata
<p><strong>Purpose:</strong> We evaluated how constant incubation temperatures affect life-history traits pre-hatching and post-hatching of the six-tubercled Amazon River turtle, <em>Podocnemis sextuberculata</em>.</p> <p><strong>Methods:</strong> We incubated eggs from natural nests at ten semi-constant temperatures between 22.26±1.01°C and 37.37±0.38°C (2013) and at six temperatures between 25.75±0.22°C and 36.17±0.15°C (2016). In 2013, we raised hatchling for 90 days to evaluate effects of temperature on early hatchling growth. We evaluated maternal effects in 2016.</p> <p><strong>Results:</strong> <em>P. sextuberculata</em> displays temperature-dependent sex determination and produces males at colder and females at warmer temperatures (TSD Ia). The estimated pivotal temperature was 33.73 ± 0.15°C and the transitional range of temperatures (TRT) 1.16 ± 0.59°C. Semi-constant temperatures below 26°C and above 38°C were lethal. Intermediate temperatures (32.25°C and 31.5°C, respectively) were optimal for hatching success and produced larger hatchlings that grew slower early in life compared to colder or warmer conditions, which produced smaller hatchlings. Warmer incubation temperatures within the optimal range (28°C-37°C) accelerated embryonic development. In contrast, comparisons of 30, 60 and 90 days-old suggests that warmer incubation temperatures reduced growth and mass gain rates post-hatching, such that incubation temperature effects on body size at emergence disappeared by 3 months of age.</p> <p><strong>Conclusions</strong>: Six-tubercled Amazon River turtles showed the highest pivotal temperature reported for any turtle. The relatively narrow TRT may limit the evolutionary potential of this vulnerable turtle in the face of global warming. Future incubation experiments at a finer scale (33°C-36°C) are warranted to refine the sex-ratio reaction norm. Field studies that monitor natural nests are imperative to evaluate conservation measures and the effect of female-biased illegal hunting and climate change. By providing data about the thermal biology of an understudied lineage of non-model species, our study helps fill gaps in our understanding of the evolution of vertebrate sex determination and its potential adaptive value.</p>
Dynamic interplay of cNHEJ and MMEJ pathways of DNA double-strand break repair during embryonic development in zebrafish.
<p><span>Fastq sequences from zebrafish embryos corresponding to 9 amplicons sequenced by </span><span>Illumina MiSeq. DNA sample were obtained from non-treated wildtype controls (ctrl) or from wildtype (WT), polq mutants, lig3 mutant or lig4 mutant injected with Cas9 protein and a pool of 5 or 4 different sgRNA (numbered from 1#1 to 5#2). Each target site corresponds to one amplicon. </span></p>
(06)-He2019A-DS0001 – Tribolium castaneum foxQ2-5' line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(06)-He2019A-DS0001 – <em>Tribolium castaneum</em> foxQ2-5' line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
Figure 3 in Embryonic development of the olive fruit fly, Bactrocera oleae Rossi (Diptera: Tephritidae), in vivo
Figure 3. Hatching of Bactrocera oleae egg by 66 h (A, B, C, and D).
FIGURE 3 in Late Paleocene examples of residual coloration and embryonic features in juvenile marine mollusks from Northwest Louisiana
FIGURE 3 caption next page.
Selected quantitative measurements of Ceratitis capitata embryonic development
<p>Selected quantitative measurements of <em>Ceratitis capitata</em> embryonic development (processed image data, Mathematica notebooks, as well as intermediate measurement and visualization files)</p>
Data from: Recurrent sublethal warming reduces embryonic survival, inhibits juvenile growth, and alters species distribution projections under climate change
The capacity to tolerate climate change often varies across ontogeny in organisms with complex life cycles. Recently developed species distribution models incorporate traits across life stages; however, these life-cycle models primarily evaluate effects of lethal change. Here, we examine impacts of recurrent sublethal warming on development and survival in ecological projections of climate change. We reared lizard embryos in the laboratory under temperature cycles that simulated contemporary conditions and warming scenarios. We also artificially warmed natural nests to mimic laboratory treatments. In both cases, recurrent sublethal warming decreased embryonic survival and hatchling sizes. Incorporating survivorship results into a mechanistic species distribution model reduced annual survival by up to 24% compared to models that did not incorporate sublethal warming. Contrary to models without sublethal effects, our model suggests that modest increases in developmental temperatures influence species ranges due to effects on survivorship.
FIG. 1 in New data on embryonic development of Halisarca dujardini Johnston, 1842 (Demospongiae, Halisarcida)
FIG. 1. — Life-history scheme of Halisarca dujardini in the White Sea.
Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation
<p>Spindle orientation is often achieved by a complex of Partner of Inscuteable (Pins)/LGN, Mushroom Body Defect (Mud)/Nuclear Mitotic Apparatus (NuMa), Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this process. Here, we identify Pins-mediated planar cell polarized divisions in several of the mitotic domains of the early <em>Drosophila</em> embryo. We found that neither planar cell polarity pathways nor planar polarized myosin localization determined division orientation; instead, our findings strongly suggest that Pins planar polarity and force generated from mesoderm invagination are important. Disrupting Pins polarity via overexpression of a myristoylated version of Pins caused randomized division angles. We found that disrupting forces through chemical inhibitors, depletion of an adherens junction protein, or blocking mesoderm invagination disrupted Pins planar polarity and spindle orientation. Furthermore, directional ablations that separated mesoderm from mitotic domains disrupted spindle orientation, suggesting that forces transmitted from mesoderm to mitotic domains can polarize Pins and orient division during gastrulation. To our knowledge, this is the first in vivo example where mechanical force has been shown to polarize Pins to mediate division orientation.</p>
Single-cell RNA sequencing of mouse embryonic cells from the oocyte, 2-cell, 4-cell, 8-cell, blastocyst, and morula stages
<p>STRT-N is a newly optimized single-cell RNA sequencing method for studies of early genome activation in mammalian preimplantation development. Single embryos from the oocyte, 2-cell, 4-cell, 8-cell, blastocyst, and morula stages were sampled for experiments and were sequenced using STRT-N method. Here is the raw data from STRTN-seq. FASTQ files are available in <a href="https://www.ebi.ac.uk/biostudies/studies/S-BSST976">BioStudies database</a>.</p>
Microscopy-based assessment of RNA polymerase II clusters during differentiation of cultured mouse embryonic stem cells
<p>This repository contains the data and MatLab analysis scripts of the analysis of RNA polymerase II clusters over the course of differentiation of cultured mouse embryonic stem cells (mESCs). The cells were induced towards differentiation by either withdrawal of lineage-inhibiting factors (-LIF) or by RHB treatment. Both treatments were carried out in duplicate in independent experiments. Time course data were obtained by collection of cells at 3, 6, 12, 24, and 48 hours following the beginning of induction. Control data from unindexed cells were obtained at 24 and 48 hours. Cells were cultured, treated, collected, and fixed at University Medical Center Göttingen by Yomna Gohar, Priya Kumar, and Carmelo Ferrai. Fixed cells were fluorescently labeled and microscopy images were recorded and analyzed at Karlsruhe Institute of Technology by Lennart Hilbert. </p> <p>To analyse date data, the raw image data are first extracted into MatLab-native files using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/MultiPosition_extraction_nd2.m">MultiPosition_extraction_nd2.m</a> script. The actual analysis is then carried out using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ClusterAnalysis.m">ClusterAnalysis.m</a> script. Example microscopy images were produced using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ExampleImages.m">ExampleImages.m</a> script. The extracted data can be reviewed using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ReviewExtractedStacks.m">ReviewExtractedStacks.m</a> script.</p> <p>This is the main repository, containing the image analysis scripts alongside the links to the raw image data used in the analysis. The raw data are too large to be stored within this single repository, and therefore can be found at the following additional addresses.</p> <p>Differentiation by withdrawal of lineage-inhibiting factors (-LIF): <a href="https://doi.org/10.5281/zenodo.8013281">10.5281/zenodo.8013281</a></p> <p>Differentiation with addition of RHB for neuronal fate induction (RHB): <a href="https://doi.org/10.5281/zenodo.8013307">10.5281/zenodo.8013307</a></p>
Data for: Behavioral evidence of olfactory imprinting during embryonic and larval stages in lake sturgeon
<p>Many migratory fishes are thought to navigate to natal streams using olfactory cues learned during early life stages. However, direct evidence for early-life olfactory imprinting is largely limited to Pacific salmon, and other species suspected to imprint show life history traits and reproductive strategies that raise uncertainty about the generality of the salmonid-based conceptual model of olfactory imprinting in fishes. Here, we studied early-life olfactory imprinting in lake sturgeon (<em>Acipenser fulvescens</em>), which have a life cycle notably different from Pacific salmon, but are nonetheless hypothesized to home via similar mechanisms. We tested one critical prediction of the hypothesis that early-life olfactory imprinting guides natal homing in lake sturgeon: that exposure to odorants during early-life stages results in increased activity when exposed to those odorants later in life. Lake sturgeon were exposed to artificial odorants (phenethyl alcohol and morpholine) during specific developmental windows and durations (limited to the egg, free-embryo, exogenous feeding larvae, and juvenile stages), and later tested as juveniles for behavioral responses to the odorants that were demonstrative of olfactory memory. Experiments revealed that lake sturgeon reared in stream water mixed with artificial odorants for as little as 7 days responded to the odorants in behavioral assays over 50 days after the initial exposure, specifically implicating the free-embryo and larval stages as critical imprinting periods. Our study provides evidence for olfactory imprinting in a non-salmonid fish species, and supports further consideration of conservation tactics such as stream-side rearing facilities that are designed to encourage olfactory imprinting to targeted streams during early life stages. Continued research on lake sturgeon can contribute to a model of olfactory imprinting that is more generalizable across diverse fish species and will inform conservation actions for one of the world's most imperiled fish taxonomic groups.</p>
Data for: The effects of embryonic incubation temperature on subsequent growth and thermal tolerance in white sturgeon throughout onset of exogenous feeding to early juveniles
<p>An organism's phenotypic characteristics can be altered by environmental variation experienced during embryonic development, potentially leading to changes that persist into adulthood. Increasing global temperatures are a current concern that may be particularly acute for species already threatened or endangered, such as the white sturgeon, <em>Acipenser</em> <em>transmontanus</em>. Given the limited information on the effects of embryonic temperatures on subsequent physiological parameters such as thermal tolerance and growth in this species, the effects of global climate change on the future of white sturgeon populations are uncertain. To investigate carryover effects resulting from early rearing temperatures, white sturgeon embryos were incubated at different environmental temperatures (T<sub>e</sub>) of 12, 15, and 18 °C until hatch, after which fish were reared at a common 15 °C for 80 days post-hatch (dph). Individuals from each temperature treatment were tested for thermal tolerance using the critical thermal maximum method (CT<sub>max</sub>) at regular intervals from 13 to 80 dph, which bridged the time from the start of exogenous feeding through the transition into early juveniles. In addition, body length measures were taken to determine the effects of embryonic T<sub>e</sub> on growth through these life stages. We found that embryonic T<sub>e</sub> affected both thermal tolerance and growth; fish that developed at 18 °C had the lowest thermal tolerance, while those that developed at 12 °C grew largest over the observation period. This research represents a window into a critical period of development during which organisms are particularly vulnerable to climatic variation. The results can inform environmental managers on the best strategies to help conserve current white sturgeon populations across their range.</p>
Insulin-like Growth Factor 1 Receptor (IGF-1R) Antibody AMG479 (Ganitumab) in Combination With the Src Family Kinase (SFK) Inhibitor Dasatinib in People With Embryonal and Alveolar Rhabdomyosarcoma
ClinicalTrials.gov study NCT03041701. IPD Sharing: YES. Countries: 1. Publications: 1.
Risk-Adapted Therapy for Young Children With Embryonal Brain Tumors, Choroid Plexus Carcinoma, High Grade Glioma or Ependymoma
ClinicalTrials.gov study NCT00602667. IPD Sharing: Not stated. Countries: 2. Publications: 3.
Data for: Behavioral evidence of olfactory imprinting during embryonic and larval stages in lake sturgeon
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Data for: The effects of embryonic incubation temperature on subsequent growth and thermal tolerance in white sturgeon throughout onset of exogenous feeding to early juveniles
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Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.