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9,597 results for “transplantation”
Adipose-derived stromal cells preserve pancreatic islet function in a transplantable 3D bioprinted scaffold
<p><span>Intra-portal islet transplantation is the method of choice for treatment of insulin dependent type 1 diabetes, but its outcome is hindered by limited islet survival due to the immunological and metabolic stress post transplantation. Adipose-derived stromal cells (ASCs) promise to improve significantly the islet micro-environment but an efficient long-term delivery method has not been achieved. We therefore explore the potential of generating ASC enriched islet transplant structure by 3D bioprinting. Here, we fabricate a double-layered 3D bioprinted scaffold for islets and ASCs by using alginate-nanofibrillated cellulose bioink. We demonstrate the diffusion properties of the scaffold and report that human ASCs increase the islet viability, preserve the endocrine function, and reduce pro-inflammatory cytokines secretion <em>in vitro</em>. Intraperitoneal implantation of the ASCs and islets in 3D bioprinted scaffold improve the long-term function of islets in diabetic mice. Our data reveals an important role for ASCs on the islet micro-environment. We suggest a novel cell therapy approach of ASCs combined with islets in a 3D structure with a potential for clinical beta cell replacement therapies at extrahepatic sites. </span></p>
Dataset and code for "Modelling the potential for peat-block transplants to restore industrially contaminated Sphagnum peatlands"
<p>Code to run simulations and dataset from HYDRUS-1D simulations used in the revised manuscript titled "Modelling the potential for peat-block transplants to restore industrially contaminated Sphagnum peatlands" submitted to Ecological Engineering</p>
T-cell receptor Vβ (TCRVB) deep sequencing on human T cells isolated from humanized mice engrafted with human PBMC and treated or not with PTCy post-transplantation
<p>Nucleotide sequence data of TCRVB sequencing performed on T cells isolated from the pre-transplantation hPBMC (donor T cells) or from mice organs at day 21 post-transplantation (injected or not with PTCy at day 3) to determine the impact of PTCy on the T cell V beta (TCRVB) receptor repertoire diversity.</p> <p>NSG mice were engrafted with human PBMC to develop xeno-GVHD and treated or not with 100 mg/kg PTCy. Spleens and lungs from 10 NSG mice per group were pooled and stained to sorted human CD4+ and CD8+ . DNA of human CD4+ and CD8+ T cells sorted from each organ and from the PBMC donor was extracted. One hundred fifty µg from each sample were used for T-cell receptor Vβ (TCRVB) deep sequencing performed by Adaptive Biotechnologies.<br> </p>
Data for: Whole-of-community invertebrate rewilding: Leaf litter transplants rapidly increase beetle diversity during restoration
<p><span>Restoration of degraded areas is now a central tool in humanity's response to continued species loss. However, restoration projects often report exceedingly slow or failed recolonization of fauna, especially dispersal-constrained groups such as invertebrates. Active interventions via reintroducing or "rewilding" invertebrates may assist recolonization and speed up the restoration of communities towards a desired target. However, invertebrate rewilding is rarely implemented during ecological restoration. Here, we studied the efficacy of invertebrate rewilding as a means of improving diversity and compositional similarities to remnant communities during restoration. Rewilding was conducted by transplanting leaf litter and soil, including associated communities of invertebrates from species-rich remnant sites into species-poor and geographically isolated revegetated farmland sites. We sampled pre- and post-rewilding invertebrate communities in remnant, rewilded revegetation, and control revegetation sites. Our data contains mesoinvertebrate (<5mm in size) community samples from 6 sites within each of our 3 treatments. We took 8 subsamples measuring 25 cm by 25 cm containing the entire mesoinvertebrate leaf litter community from each site across 4 different seasons. The first season was a pre-treatment baseline measurement and was taken in November 2018. Thereafter, we sampled 7-, 18-, and 27- months post-rewilding. Our data contains a range of traits for each morphospecies (Size in mm, trophic group, and whether they were winged or flightless) and two environmental variables measured at each site (Volumetric water content and elevation). We included these for our Hierarchical Modelling of Species Community (HMSC) approach to determine whether biological properties of species or environmental variables were driving species distributions. Beetle (Coleoptera) morphospecies richness increased rapidly in rewilded sites and was indistinguishable from remnant communities as early as 7 months post-rewilding. Beetle community similarity in the rewilding sites significantly deviated from the control sites 27 months post-rewilding, however, remnant communities remained distinct over the study timeframe. Establishment success varied as other taxa did not respond as consistently as beetles within the study timeframe. Our results demonstrate that the relatively simple act of transplanting leaf litter can result in comparatively large increases in morphospecies richness during restoration in a short timeframe.</span></p>
Assessing moss transplant methods to enhance Sphagnum moss recovery in post-wildfire hydrophobic peat
<p><strong>Wildfire is the dominant disturbance in northern peatlands and can release large quantities of carbon to the atmosphere through combustion. Post-fire peat hydrophobicity can inhibit moss regeneration, thereby decreasing the potential for post-fire carbon sequestration. To investigate how to enhance post-fire recovery we assessed two moss restoration methods (plugs and fragments) in an Alberta poor fen two and three years following wildfire. P<strong>ost-fire peat hydrophobicity and moss regeneration was characterized in four surface cover types: Severely Burned Feather moss hollows (SB-F), Severely Burned Sphagnum fuscum hummocks (SB-S), Lightly Burned S. fuscum hummocks (LB-S), and Lightly Burned Feather moss lawns (LB-F). We then <strong>conducted experiments testing the success of moss plugs and fragments of varying moss species at LB-F and SB-F surface covers, which had high hydrophobicity and low post-fire moss recovery.</strong></strong></strong></p>
Data from: Cedrus libani survival and growth data from a transplant experiment and natural forests in northern Lebanon
<p><strong>Aims</strong></p> <p>The <em>Cedrus</em> dataset was set up to assess the climatic niche breadth of <em>Cedrus libani –</em> a relict mountain species <em>–</em> by comparing field observations and transplant experiment measurements within and beyond the elevational range limits of <em>Cedrus</em> under natural conditions.</p> <p><strong>Location</strong></p> <p>Lebanon - Near East - Mediterranean region</p> <p><strong>Methods</strong></p> <p>The transplant experiment included 8 common gardens at altitudes ranging from 110 to 2330 m, within and far beyond the warm and cold limits of Cedar distribution under natural conditions. It was set up in 2018, and monitored for three years until fall 2021 to study survival and growth of the Cedar of Lebanon (<em>Cedrus libani</em>) and 3 potentially competing species (<em>Quercus calliprinos, Quercus infectoria and </em><em>Pinus</em> <em>brutia</em>). Field observations of Cedar presence/absence were carried out on 1023 sites.</p> <p><strong>Results</strong></p> <p>We observed surprisingly high survival and growth rates of Cedar at elevations well below its natural range in Lebanon. Below its elevational limit of 1300 m under natural conditions, water stress limited the survival of juvenile Cedars at elevations below 500 m, and its low competitiveness below 900 m explained its absence between 500 and 900 m elevation. On the other hand, cold temperature and water stress limited its survival at elevations slightly above the observed natural upper elevational limit of 1830 m.</p> <p><strong>Main conclusions</strong></p> <p>The experimental setup demonstrated that the elevational range suitable for the growth and survival of the Cedar of Lebanon is twice as large as the range within which Cedar is observed today under natural conditions. The high survival rate beyond the lower limit of its natural range raises hope for its resilience to ongoing climate warming. If this pattern were common to other mountain species, it would challenge predictions of massive extinction linked to climate change, and pave the way for promoting adaptive actions such as competition management to improve their survival.</p>
Analysis results for association study of long-term kidney transplant rejection using whole-exome sequencing
<p>Association study results for long-term kidney transplant rejection. Single-variant association results are provided as Plink output files. Meta-analysis results are provided as METAL output files. FDR results are sorted lists of the top association result from random sample label permutations and are included with the plink and meta-analysis results. SKAT and GSEA results are provided for gene and pathway level analyses, respectively.</p>
Cryopreservation and transplantation of common carp spermatogonia - spermatogonia viability dataset
<p><strong>Dataset of spermatogonia viability assessment from optimization of cryopreservation and vitrification protocols.</strong></p>
Impact of CYP3A5 Genotypes on Tacrolimus Pharmacokinetics in Colombian Liver Transplant Patients
<p><strong><span>Impact of CYP3A5 Genotypes on Tacrolimus Pharmacokinetics in Colombian Liver Transplant Patients</span></strong></p>
Experimental treatments and survival of transplanted harvester ant colonies
<p>In sessile organisms such as plants and benthic invertebrates, founding propagules typically suffer extremely high rates of mortality due to both extrinsic and intrinsic factors. Many social insect species share similarities with these groups, but factors influencing early colony survival are relatively unstudied. </p> <p>We used a field experiment to measure the importance of environmental quality relative to intrinsic colony properties in the harvester ant, Pogonomyrmex occidentalis, by monitoring the survival of 584 experimental colonies. Colony survival was primarily determined by intrinsic factors. Multiple mating by the queen and larger colony size at the time of transplant increased survival, but queen size, maternal lineage and the composition of plant species in the vicinity of the colony did not. Food supplementation increased survival significantly when natural food was scarce, but was not consistently beneficial, in contrast to predictions. </p> <p>Our results emphasize the general importance of rapid growth and early attainment of large size in the survival of sessile species. However, attributes specific to ants, their sociality and mating system, also strongly affected survival. Colonies with multiply-mated queens were more likely to survive over a wide range of circumstances, highlighting the importance of this trait even at the early stages of colony life.</p> <p>These are data to support the paper "The benefits of being big and diverse: early colony survival in harvester ants." The data are the experimental traits of the colonies used for transplantation (size and lineage of the queen, size of the colony, mating status, food treatment, local vegetation), the characteristics of the transplant itself (year of transplant and the transplant set) as well as the experimental results (the duration of survival and whether the colony survived until the end of the experiment).</p>
Cardiac patch transplantation instruments for robotic minimally invasive cardiac surgery: initial proof-of-concept designs and surgery in a porcine cadaver
<p>BACKGROUND: Damaged cardiac tissues could potentially be regenerated by transplanting bioengineered cardiac patches to the heart surface. To be fully paradigm-shifting, such patches may need to be transplanted using minimally invasive robotic cardiac surgery (not only traditional open surgery). Here, we present novel robotic designs, initial prototyping and a new surgical operation for instruments to transplant patches <em>via </em>robotic minimally invasive heart surgery. METHODS: Robotic surgical instruments and automated control systems were designed, tested with simulation software and prototyped. Surgical proof-of-concept testing was performed on a pig cadaver. RESULTS: Three robotic instrument designs were developed. The first (called “Claw” for the claw-like patch holder at the tip) operates on a rack and pinion mechanism. The second design (“Shell-Beak”) uses adjustable folding plates and rods with a bevel gear mechanism. The third (“HeartStamp”) utilizes a stamp platform protruding through an adjustable ring. For the HeartStamp, rods run through a cylindrical structure designed to fit a uniportal Video-Assisted Thorascopic Surgery (VATS) surgical port. Designed to work with or without a sterile sheath, the patch is pushed out by the stamp platform as it protrudes. Two instrument robotic control systems were designed, simulated <em>in silico</em> and one of these underwent early ‘sizing and learning’ prototyping as a proof-of-concept. To reflect real surgical conditions, surgery was run “live” and reported exactly (as-it-happened). We successfully picked up, transferred and released a patch onto the heart using the HeartStamp in a pig cadaver model. CONCLUSION: These world-first designs, early prototypes and a novel surgical operation pave the way for robotic instruments for automated keyhole patch transplantation to the heart. Our novel approach is presented for others to build upon free from restrictions or cost – potentially a significant moment in myocardial regeneration surgery which may open a therapeutic avenue for patients unfit for traditional open surgery.</p>
Raw data fort the article: FLUOROSCOPY-GUIDED BILIARY PROCEDURES IN A PREGNANT, LIVER TRANSPLANT PATIENT: FETUS RADIATION PROTECTION
<p>We report three cases of clinically necessary, fluoroscopy-guided, percutaneous biliary procedures performed safely in a pregnant, liver transplant recipient using three different angiography suites. The uterine cumulative equivalent dose was 0.25 mSv, a value obtained by adding the doses of the three procedures described above, and which is relatively low when compared with the naturally occurring background radiation exposure for a 9-month pregnancy (~0.5-1 mSv). Our experience shows that staff knowledge, awareness and liaison promote the application of all dose reduction strategies possible while still achieving the clinical aim despite using different angiographic equipment.</p>
Drivers and Determinants of Strain Dynamics Following Faecal Microbiota Transplantation
<p>Faecal microbiota transplantation (FMT) is an efficacious therapeutic intervention, but its clinical mode of action and underlying microbiome dynamics remain poorly understood. Here, we analysed the metagenomes associated with 142 FMTs, in a time series-based meta-study across five disease indications. We quantified strain-level dynamics of 1,089 microbial species based on their pangenome, complemented with 47,548 newly constructed metagenome-assembled genomes. Using subsets of procedural-, host- and microbiome-based variables, LASSO-regularised regression models accurately predicted the colonisation and resilience of donor and recipient microbes, as well as turnover of individual species. Linking this to putative ecological mechanisms, we found these sets of variables to be informative of the underlying processes that shape the post-FMT gut microbiome. Recipient factors and complementarity of donor and recipient microbiomes, encompassing entire communities to individual strains, were the main determinants of individual strain population dynamics, and mostly independent of clinical outcomes. Recipient community state and the degree of residual strain depletion provided a neutral baseline for donor strain colonisation success, in addition to inhibitive priority effects between species and conspecific strains, as well as putatively adaptive processes. Our results suggest promising tunable parameters to enhance donor flora colonisation or recipient flora displacement in clinical practice, towards the development of more targeted and personalised therapies.</p>
Data for: Testing for fitness epistasis in a transplant experiment identifies a candidate adaptive locus in Timema stick insects
<p>Identifying the genetic basis of adaptation is a central goal of evolutionary biology. However, identifying genes and mutations affecting fitness remains challenging because a large number of traits and variants can influence fitness. Selected phenotypes can also be difficult to know <em>a priori</em>, complicating top-down genetic approaches for trait mapping that involve crosses or genome-wide association studies. In such cases, experimental genetic approaches, where one maps fitness directly and attempts to infer the traits involved afterward, can be valuable. Here, we re-analyse data from a transplant experiment involving <em>Timema</em> stick insects, where five physically clustered SNPs associated with cryptic body colouration were shown to interact to affect survival. Our analysis covers a larger genomic region than past work and revealed a locus previously not identified as associated with survival. This locus resides near a gene, <em>Punch</em> (<em>Pu</em>), involved in pteridine pigments production, implying that it could be associated with an unmeasured colouration trait. However, by combining previous and newly obtained phenotypic data, we show that this trait is not eye or body colouration. We discuss the implications of our results for the discovery of traits, genes, and mutations associated with fitness in other systems, as well as for supergene evolution.</p>
HLA epitopic mismatch and risk of developing Donor Specific Antibodies in pediatric kidney transplantation
<p>The aim of this study was to evaluate the correlation between epitopic HLA mismatches and the risk of developing DSA in pediatric kidney recipients. There are very few publications concerning epitope HLA mismatch in pediatric kidney transplantation although this topic has been growing in scientific articles in recent years and is promising in adult recipients. The majority of children with chronic renal failure will require multiple kidney transplants during their lifetime and it is therefore important that we can limit the risk of antibody development in order to give them the best chance of survival for their future kidney transplants.</p>
[Data S2] Microbiota dictate T cell clonal selection to augment graft-vs-host disease after stem cell transplantation
<p><strong>Data</strong> <strong>S2. GLIPH2 hits for all recipient pairs amongst B6 to B6D2F1 transplants with or without antibiotic exposure and 900cGy vs. 1300cGy TBI conditioning. </strong>For all TCRs within each recipient pair (12 mice total, 66 pairs for spleen; 15 pairs for SILP analysis), GLIPH2 specificity groups are generated as described in methods.</p>
[Data S1] Microbiota dictate T cell clonal selection to augment graft-vs-host disease after stem cell transplantation
<p><strong>Data S1. ALICE hits for all recipient pairs amongst B6 to B6D2F1 transplants with or without antibiotic exposure and 900cGy vs. 1300cGy TBI conditioning. </strong>For each recipient pair (12 mice total, 66 pairs for spleen; 15 pairs for SILP analysis), all ALICE hits generated as described in methods.</p>
[Github Sample Dataset] Microbiota dictate T cell clonal selection to promote graft-vs-host disease after stem cell transplantation
<p>[Github Sample Dataset]. This is an accompanying data set for the github repository "<em>https://github.com/acyeh-lab/2023/tree/main/tcr-simulation" referred to in "</em>Non-genetic determinants of clonotypic T cell expansion following stem cell transplantation" by Yeh AC et al.</p>
A longitudinal study of DNA and RNA viruses plasma detection in allogeneic hematopoietic stem cell transplant recipients
<p><span><strong>Background:</strong> </span><span>Viral infections are among the most common complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and can be associated with transient or sustained viremia. Besides viruses that are common causes of infection, metagenomics revealed the presence of several novel viruses and variants that are overlooked in clinical routine and represent potential sources of unrecognized systemic infections</span><span>. Our aim was to describe the prevalence and the dynamics of 17 DNA and 3 RNA viral infections using (r(RT-)PCR) assays on plasma samples of adult allo-HSCT recipients over a one-year period after HSCT.</span></p> <p><strong><span>Methods:</span></strong><span> 109 adult patients that received a first allo-HSCT from 1<sup>st</sup> March 2017 to 31<sup>st</sup> January 2019 we included in this</span> <span>longitudinal observational monocentric cohort study</span><span>.</span> <span>17 DNA and 3 RNA viral species were screened with qualitative and/or quantitative r(RT)-PCR assays performed on plasma samples </span><span>collected at five time-points (day 0 and 30 days, 3 months, 6 months and one year after HSCT). </span></p> <p><strong><span>Results: </span></strong><span>TTV was the most prevalent with an increasing prevalence to 96% of patients at 3 months. HPgV-1 prevalence ranged from 26 to 36% of patients. TTV and HPgV-1 plasma viral load peaked at month 3 (TTV: median 3.29E5 copies/ml [range, 3.37E2 to 4.06E9 copies/ml]; HPgV-1: median 1.18E6 copies/ml [range, 2.61E3 to 4.49E7 copies/ml]). Among <em>Polyomaviridae</em>, BKPyV, JCPyV, MCPyV, HPyV6 and 7 were detected in ≥10% of patients at ≥1 time-point. HPyV6 and HPyV7 prevalence reached 27% and 12% of patients at month 3. Among those, 41% and 63% had quantifiable viral loads, with median viral loads above 1E3copies/ml and results may suggest HPyV6 sustained viremia. Co-detections were frequent, in particular at 3 months with ≥2 viruses detected in 72% of patients. </span></p> <p><strong><span>Conclusion: </span></strong><span>Our study confirms that TTV and HPgV-1 infections are highly prevalent and that infection may be sustained up to one year after allo-HSCT. Our systematic and large strategy of screening also revealed diverse and numerous co-detections, and that several novel <em>Polyomaviridae</em> (MCPyV, HPyV6/7) that are overlooked in clinical routine are as or more frequently detected compared to classical culprits. Our results underscores the need for further studies investigating the clinical impact of classical culprits together with other viruses in particular novel <em>Polyomaviridae</em> and HPgV-1. </span></p>
Raw Data for the article: Neurological Screening in Elderly Liver Transplantation Candidates: A Single Center
<p><strong>Background: </strong>Cerebral small vessels disease (cSVD) is an age-related disorder and risk factor for stroke and cognitive/motor impairments. Neurological complications (NCs) are among the causes of adverse outcomes in older liver transplant recipients. This study sought to determine whether cSVD predicts acute NCs in over 65-year-old liver transplant patients.</p> <p><strong>Methods: </strong>Data were collected, from a retrospective medical chart review, of 22 deceased donor liver transplant recipients aged 65 years or older with a pre-operative brain magnetic resonance imaging (MRI). We used the Fazekas score (0-3) as a quantitative measurement of the vascular lesion load seen in the MRI. We analyzed all post-operative acute NCs occurring during the hospital stay and any other non-NC.</p> <p><strong>Results: </strong>cSVD was recognized in all patients. Neurological complications (NCs) occurred in 18.1% of patients with toxic-metabolic encephalopathy the most frequent diagnosis (13.64%). More severe cSVD was associated with seizures (<em>p</em> = 0.0362), longer hospital stay (<em>p</em> 0.0299), and disability (<em>p</em> 0.0134). In our elderly cohort, hepatic encephalopathy (HE) (<em>p</em> 0.0287) and ascites (<em>p</em> 0.0270) were predictors of NCs after liver transplantation. Ascites and/or variceal bleeding and severity of liver disease were associated with adverse post-operative outcomes. The small sample size limited the statistical analysis power.</p> <p><strong>Conclusions: </strong>We present the preliminary data of a single-center retrospective study aimed at understanding the cSVD role on NCs and non-NCs after a liver transplantation in elderly patients. This would encourage a more appropriate multicenter prospective study that will definitely confirm if a neurological screening in old age liver transplant candidates is appropriate.</p>
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.