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FIGURE 1 in Description of a new Notocomplana species (Platyhelminthes: Acotylea), new combination and new records of Polycladida from the northeastern Sea of Japan, with a comparison of two different barcoding markers

FIGURE 1. Notocomplana hagiyai sp. nov. A. Dorsal view in the living state, ICHUM5267 (holotype). B. Ventral view in the living state, ICHUM5267 (holotype). C. Eyespots in the living state, ICHUM5267 (holotype). D. Sagittal section through the male copulatory apparatus, anterior to the right, ICHUM5267 (holotype). E. Cross section through the male copulatory apparatus, ICHUM5272 (paratype). F. Sagittal section through the female copulatory apparatus, anterior to the right, ICHUM5267 (holotype). Abbreviations: cec, cerebral eye cluster; cov, common oviduct; exc, extra-vesicular gland's canal; fg, female gonopore; ied, intra-prostatic ejaculatory duct; ld, Lang's duct; lv, Lang's vesicle; ma, male atrium; mg, male gonopore; ped, post-prostatic ejaculatory duct; ph, pharynx; pp, penis papilla; pv, prostatic vesicle; sd, sperm duct; sv, seminal vesicle; tec, tentacular eye cluster; v, vagina.

FIGURE 5 in First record of the genus Bunops Birge, 1893 (Cladocera: Macrothricidae) in the Neotropical highlands of Mexico with a detailed study of morphology and DNA barcodes

FIGURE 5. Bunops cf. serricaudata from Chipila, Tlaxcala State, Mexico, adult male. A, habitus. B, A1, external view. C, A1, internal view. D, detail of the internal anterior part with two leaf-shape aesthetascs, A1. E, posterior internal part of A1, with one seta and rows of spines. F, internal part of the valves, middle part. G, postabdomen. H, claws. I, hook in thoracic limb I. J, detail of the tip in the hook of limb I, with three rows of spines.

FIGURE 1 in First record of the genus Bunops Birge, 1893 (Cladocera: Macrothricidae) in the Neotropical highlands of Mexico with a detailed study of morphology and DNA barcodes

FIGURE 1. Bunops cf. serricaudata from Chipila, Tlaxcala State, Mexico. A, habitus, parthenogenetic female. B, habitus, ephippial female. C, antennules (scale bar 80 µm). D, tip of antennulae with aesthetascs (scale bar 40 µm).

FIGURE 6 in First record of the genus Bunops Birge, 1893 (Cladocera: Macrothricidae) in the Neotropical highlands of Mexico with a detailed study of morphology and DNA barcodes

FIGURE 6. Neighbor Joining simplified tree of all macrotricids found in BOLD. The country of collection (when known) and the BIN number in this database are listed after the species or genus name.

FIGURE 2 in First record of the genus Bunops Birge, 1893 (Cladocera: Macrothricidae) in the Neotropical highlands of Mexico with a detailed study of morphology and DNA barcodes

FIGURE 2. Bunops cf. serricaudata from Chipila, Tlaxcala State, Mexico. A, antenna (scale bar: 310 µm). B, detail of the distal part of the basipod from A (scale bar: 80 µm). C, postabdomen (scale bar: 200 µm). D, seta natatoria (scale bar 80 µm). E, detail of the armature in the margin of the valves (scale bar 40 µm).

FIGURE 3. COX I in DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)

FIGURE 3. COX I gene tree resulting from NJ analysis of the full data set, including 56 Sphecius sequences. Bootstrap values greater than 70% are provided above the branches. Haplotype symbols match those used in Fig. 1. Abbreviations after each name indicate the state where the individual was collected (and gc indicates Grand Canyon National Park). When multiple individuals from a state were used in the analysis, they are numbered.

FIGURE 2. COX I in DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)

FIGURE 2. COX I gene tree resulting from NJ analysis of the BBNP data set. Bootstrap values greater than 70% are provided above the branches. Haplotype symbols match those used in Fig. 1. Note that S. speciosus and the majority of S. convallis individuals have the same haplotype. The different symbols here emphasize that these are different species.

FIGURE 1 in DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)

FIGURE 1. Geographic distribution of mitochondrial haplotypes for individuals of the three Sphecius species sampled from the USA. Approximate localities for all 54 individuals are shown. (Note: S. spectabilis does not occur in the USA; the two individuals sampled were Argentinean).

FIGURE 4. COX 1 in DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)

FIGURE 4. COX 1 gene tree resulting from maximum likelihood analysis of the pruned data set, with duplicate Sphecius sequences removed. Bootstrap values greater than 70% are provided above the branches. Haplotype symbols match those used in Fig. 1.

FIGURE 2 in DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

FIGURE 2. Consensus tree of Clistoabdominalis and Nephrocerus relationships inferred from cox1 data (from 799 most parsimonious trees).

FIGURE 1 in DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

FIGURE 1. Neighbour joining tree of Clistoabdominalis and Nephrocerus relationships inferred from cox1 data. Numbers above the branch are bootstrap values. Asterisks indicate branches that are incongruent with respect to previous morphological hypotheses of Skevington (2001, 2005).

FIGURE 4 in DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

FIGURE 4. Consensus tree of Clistoabdominalis relationships inferred from morphological data (reduced taxon dataset to reflect the molecular taxa used in this study; using matrix from Skevington (2001)) (from two most parsimonious trees). Numbers above the branches are Bremer support values.

FIGURE 3 in DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

FIGURE 3. Single successively weighted tree of Clistoabdominalis and Nephrocerus relationships inferred from cox1 data.

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FIGURE 5 in Species status of Bombus monticola Smith (Hymenoptera: Apidae) supported by DNA barcoding

FIGURE 5. The character "shape and pubescence of basitarsus in B. lapponicus (5 A) and Bombus monticola (5 B). The line through the diverging transversely directed hair whorls (a), and the maximal width of the basitarsus (b).

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FIGURE 6 in Species status of Bombus monticola Smith (Hymenoptera: Apidae) supported by DNA barcoding

FIGURE 6. Neighbor-joining tree (based on the Kimura 2-parameter model) illustrating genetic distances within and among Bombus lapponicus and B. monticola, with B. polaris as an outgroup. Numbers near the nodes indicate bootstrap support. Support values less than 90 are not shown. For each individual, the scientific name, according to morphological characters, are followed by the GenBank accession number and sampling locality.

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FIGURE 1 in DNA barcoding confirms the presence of Hydria cervinalis (Scopoli, 1763) in the Iberian Peninsula (Lepidoptera: Geometridae: Larentiinae)

FIGURE 1. Female genitalia of Hydria cervinalis (Scopoli) with minor differences noted. Genital prep. Slide G760.

Figure 6 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species

Figure 6. Histogram of pairwise distances divergence for Atlantoscia species (A) and automatic partition of the cytochrome c oxidase subunit I data set of seven Atlantoscia species (B) as generated by automatic barcode gap discovery. The lower distance values (<0.05) represent intraspecific divergences, whereas higher distance values (> 0.12) represent interspecific divergences. Dist., distance.

Figure 4 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species

Figure 4. Atlantoscia meloi sp. nov., male paratype MZUSP 32628. A, pereopod 1; B, pereopod 7; C, uropod; D, genital papilla; E, pleopod 1; F, pleopod 2; G, pleopod 3 exopod; H, pleopod 4 exopod; I, pleopod 5 exopod.

Figure 3 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species

Figure 3. Atlantoscia meloi sp. nov., male holotype MZUSP 32627. A, habitus, dorsal view; male paratype MZUSP 32628; B, scale-seta; C, cephalothorax, frontal view; D, noduli laterales coordinates d/c; E, noduli laterales coordinates b/c; F, epimera 4; G, pleonite 4 and 5, pleotelson; H, antennule; I, antenna; J, left mandible; K, right mandible; L, maxillula; M, maxilla; N, maxilliped.

Figure 1 in Taxonomy and molecular phylogeny of the Neotropical genus Atlantoscia (Oniscidea, Philosciidae): DNA barcoding and description of two new species

Figure 1. Atlantoscia inflata sp. nov., male holotype MZUSP 32622. A, habitus, dorsal view; male paratype MZUSP 32623; B, scale-seta; C, cephalothorax, frontal view; D, cephalothorax, dorsal view; E, noduli laterales coordinates d/c; F, noduli laterales coordinates b/c; G, pleotelson; H, antennula; I, antenna; J, left mandible; K, right mandible; L, maxillula, outer endite; M, maxilla; N, maxilliped.