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Raw ONT pod5 data: Barcoded D2O Aminobacter P9b DNA

Aminobacter P9b samples were incubated on a 100% D2O, 30% D2O and 0% D2O medium, respectively. Cells were harvested by centrifugation. Pellets were resuspended and DNA extracted. The DNA was barcoded using the ONTs native barcoding kit, prepared with the SQK-LSK109 protocol, and sequenced on a MinION with an r9.4.1 flowcell. For barcoding, we used the EXP-NBD114 Barcodes 14, 15, and 16: BC 14: 0% D2O BC 15: 30% D2O BC 16: 100% D2O

FIGURES 52–57 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 52–57. Localities of Nymphomyia alba Tokunaga (52–53), N. kaluginae Makarchenko (54), N. levanidovae Rohdendorf et Kalugina (55), and N. kannasatoi Makarchenko et Gunderina (56–57). 52, Monobe River, Honshu, Japan (photo by S. Sato); 53, Prozrachnyi Stream, Kunashir Island, Russia (photo by M. Astakhov); 54, Bolshoi Garmakan River, Zeya River basin (photo by E. Makarchenko); 55, Kedrovaya River, South Primorye (photo by E. Makarchenko); 56, Kanna River, Honshu, Japan (photo by S. Sato); 57, Belaya River, South Sakhalin (photo by E. Makarchenko).

FIGURE 58 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURE 58. Ultrametric Bayesian inference (BI) tree inferred from the cytochrome c oxidase I (COI) gene fragment and the results of species delineation of the genus Nymphomya Tokunaga, 1932. Bayesian posterior probabilities (higher than 0.7) are given above the tree nodes. Vertical bars correspond to molecular operational taxonomic units by ASAP, mPTP, GMYC. The sequences obtained in this study are in bold.

FIGURES 43–45 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 43–45. Adult male (43) and female (44–45) terminalia of Nymphomyia levanidovae Rohdendorf et Kalugina, in ventral view. For abbreviations see Figs. 11–16, 30–42. Scale bars—50 μm.

FIGURES 46–51 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 46–51. Localities of Nymphomyia orientalis orientalis subsp. nov. (46), N. orientalis makcha subsp. nov. (47), N. rohdendorfi Makarchenko (48–51). 46, Polovinka River, Amur River basin (photo by N. Yavorskaya); 47, Makcha River, Zeya River basin (photo by E. Makarchenko); 48, Intrigan River, Kolyma River basin (photo by E. Khamenkova); 49, Kalanegir River, Katun River basin), of Altai Mountains (photo by A. Kotovshchikov); 50–51, Khurendya River, Kolyma River basin, adults wait out bad weather on coastal rocks (photo by A. Krasheninnikov).

FIGURES 35–37 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 35–37. Adult male (35) and female (36–37) terminalia of Nymphomyia kaluginae Makarchenko. 35–36, in ventral view; 37, in lateral view. ae, aedeagus; ap, anal point; ce, cerci; gs, gonostylus; gx, gonocoxite; pp, paratergal projection. Scale bar—50 μm.

FIGURES 21–29 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 21–29. Adult male terminalia of Nymphomyia kannasatoi Makarchenko et Gunderina from Japan (21–28) and Sakhalin Island (29). 21, paratergal projection; 22, 29, in ventral view; 23, in dorsal view; 24–28, gonostylus in varies positions. Scale bars—50 μm.

FIGURES 38–42 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 38–42. Adult male (38–39) and female (40–42) terminalia of Nymphomyia alba Tokunaga. 38, in dorsal view; 39, 41, in lateral view; 40, sternite IX process; 42, in ventral view. ae, aedeagus; ce, cerci; gs, gonostylus; gx, gonocoxite; pp, paratergal projections; sp, sternite IX median process. Scale bars—50 μm.

FIGURES 30–34 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 30–34. Adult female terminalia of Nymphomyia kannasatoi Makarchenko et Gunderina from Japan (30–33) and Sakhalin Island (34). 30, 32–33, in ventral view; 31, in dorsal view; 34, ventral margin and fingerlike projections of sternite VIII. pp—paratergal projections, vp—ventral projection, fp—fingerlike projection, vm—ventral margin, ce—cerci. Scale bars—50 μm.

FIGURES 11–14 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 11–14. Terminalia of adult females of Nymphomyia orientalis orientalis subsp. nov. (11, 13) and N. orientalis makcha subsp. nov. (12, 14) in ventral view. ce, cerci; ivl, inner ventral lobe; pp, paratergal projection. Scale bars—50 μm.

FIGURES 17–20 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 17–20. Female terminalia of Nymphomyia rohdendorfi Makarchenko in ventral view from Anyuradat Stream (Kolyma River basin) (paratype) (17), Intrigan River (Kolyma River basin) (18), Khurendya River (Kolyma River basin) (19) and Altai Mountains (20).

FIGURES 15–16 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 15–16. Adult male (15) and female (16) terminalia of Nymphomyia rohdendorfi Makarchenko in ventral view. fp, fingerlike projection; ivl, inner ventral lobe. Scale bar—50 μm.

FIGURES 8–10 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 8–10. Adult male terminalia of Nymphomyia orientalis orientalis subsp. nov. in ventral view (8) and in dorsal view (9), eggs on anal segments of male and female in copula (10). Scale bars—50 μm.

FIGURES 3–7 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURES 3–7. Adult male of Nymphomyia orientalis orientalis subsp. nov. 3, head in dorsal view; 4, head in ventral view; 5, head in lateral view; 6, terminal antennal sensilla; 7, antenna. R, rostrum; L, labium; ts, terminal antennal sensilla. Scale bar for Figs. 3–5, 7–50 μm; for Fig. 6–20 μm.

FIGURE 1 in Review of archaic nymphomyiids (Diptera, Nymphomyiidae) of the Russian Far East and bordering territories, with describing of new taxa and DNA barcoding of known species

FIGURE 1. Swarming of adults Nymphomyia kannasatoi Makarchenko et Gunderina in Kanna River, Japan (photo by S. Sato).

Figure 2 in The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

Figure 2: Rhodophyta data by country from Taxonomy page on the Barcode of Life Data System (BOLD). Source: http://www.boldsystems.org/index.php/TaxBrowser_Home

Figure 6 in The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

Figure 6: Pinnate fronds of Caulerpa taxifolia var. distichophylla growing among the white flabellate blades of Padina sp. Photo taken in Maltese waters in June 2017.

Figure 1 in The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

Figure 1: Polygon used to delineate the Mediterranean Sea whilst searching for records in the Barcode of Life Data System (BOLD). Source: http://www.boldsystems.org/

Figure 5 in The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

Figure 5: DNA barcoding of macroalgae by Mediterranean country: Italy, France, Croatia, Spain and Greece lead in the literature-based results.

Figure 4 in The current state of DNA barcoding of macroalgae in the Mediterranean Sea: presently lacking but urgently required

Figure 4: DNA barcoding by country obtained from the literature-based results of 121 papers: USA, Canada, Korea, Australia, Chile and France lead in the number of barcodes world-wide.