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Fig. 3 in Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia

Fig. 3. Phylogenetic relationships between Trypanosoma sp. ANU2 and other members of the Trypanosoma genus. Maximum likelihood tree is shown. Neighbour-joining and maximumlikelihood bootstrap support followed by Bayesian posterior probability is shown at nodes, respectively. Genbank accession numbers follow species/genotype description. Trypanosoma species/genotypes isolated in Australia are in bold. Scale bar represents substitution per site.

Fig. 6 in Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia

Fig. 6. Principle Coordinates Analysis (PCoA) plots demonstrating relationship between Trypanosoma spp. ZOTUs and host species. Dissimilarity matrices were generated using sqrt transformed Bray-Curtis distances to show distance between abundance of ZOTUs between host marsupial species; the woylie (blue squares) and brushtail possum (red circles). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia

Fig. 2. Abundance and diversity map of Trypanosoma copemani genotype 1 (G1) and genotype 2 (G2) positive samples. ZOTUs were sorted into G1 or G2 based on phylogenetic inference shown in rows, while columns are individual marsupial blood samples from infected individuals. The map represents samples separated by host species, which were WOY = woylie, or BTP = brushtail possum. Grayscale indicates number of sequences obtained from that sample as shown in the scale of intensity on the right (log transformed abundance).

Fig. 5 in Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia

Fig. 5. Trypanosoma spp. polyparasitism in 70 blood samples taken from marsupials in the Upper Warren Region. Marsupial species include: woylie (WOY), brushtail possum (BTP) and chuditch (CHU). Trypanosoma spp. include; C = Trypanosoma copemani, V = T. vegrandis, N = T. noyesi, G = T. gilletti, A = T. sp. ANU2, I = T. irwini, AT = T. sp. AAT, U = unknown, AA = T. avium, and CR = Crithidia spp.

Fig. 4 in Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia

Fig. 4. Abundance and diversity map of Trypanosoma spp. ZOTUs in different marsupial blood samples assigned to species groups shown in rows, while columns are individual blood samples. Samples are separated by host species including; WOY = woylie (34), CHU = chuditch (3), and BTP = brushtail possum (33). The colour scale indicates increasing number of sequences obtained from that sample, in that species, as shown in the intensity bar on the right. Species include; C = Trypanosoma copemani, V = T. vegrandis, N = T. noyesi, G = T. gilletti, A = T. sp. ANU2, I = T. irwini, AT = T. sp. AAT, U = unknown, AA = T. avium, and CR = Crithidia spp. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Next generation sequencing reveals widespread trypanosome diversity and polyparasitism in marsupials from Western Australia

Fig. 1. Phylogenetic relationships between Trypanosoma spp. ZOTUs assigned to nine species groups compared to 24 representative reference strains downloaded from Genbank. Phytomonas serpens and Leptomonas sp. were used as outgroups. Bayesian analysis was used to produce tree topology and posterior probability is shown at nodes. Scale bar represents substitution per site.

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Fig. 1 in Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia

Fig. 1. Bayesian phylogenetic reconstruction of the novel Babesia and Theileria spp. identified in ticks from brushtail possums and bandicoots using a multiple nucleotide alignment of 1701 bp at the 18S locus. Inset trees were produced on the basis of a shorter alignment (856 bp) with the inclusion of (a) B. macropus and (b) T. penicillata, T. brachyuri, and T. fuliginosus. Bold represents sequences identified in this study. GenBank accession numbers are shown in parentheses. Node labels represent posterior probabilities.

FIGURES 1362-1365. Pison suspiciosum F in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1362-1365. Pison suspiciosum F. Smith. (1362) Clypeus of female from Sabah; (1363) Clypeus of female from Taiwan; (1364) Male clypeus; (1365) Upper frons of female.

FIGURES 1350-1355 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1350-1355. Pison reichingeri Kohl. (1350) Female clypeus and mandibles; (1351) Clypeus and mandibles of male from Fiji; (1352) Clypeus and mandibles of male from Samoa; (1353) Upper frons of female; (1354) Propodeal dorsum of female; (1355) Female mesopleuron.

FIGURES 1314-1318 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1314-1318. Pison mariannense Yasumatsu (female, male holotype). (1314) Female clypeus and mandibles; (1315) Male clypeus (1316) Propodeal dorsum of female; (1317) Propodeal dorsum of male; (1318) Left forewing of holotype. FIGURE 1319. Collecting localities of Pison mariannense Yasumatsu.

FIGURES 1372-1375 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1372-1375. Pison trukense Yasumatsu. (1372) Female clypeus and mandibles; (1373) Male clypeus and mandibles; (1374) Upper frons of male; (1375) Propodeal dorsum of male. FIGURE 1376. Collecting localities of Pison trukense Yasumatsu.

FIGURE 1370 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURE 1370. Pison tosawai Yasumatsu, male. (1370) Clypeus. FIGURE 1371. Collecting localities of Pison tosawai Yasumatsu.

FIGURES 1257-1259 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1257-1259. Pison novabritanicae Tsuneki, male. (1257) Sternum VIII (ventral surface); (1258) Genitalia in dorsal view; (1259) Genitalia in lateral view.

FIGURES 1326-1328 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1326-1328. Pison nigellum Krombein, male. (1326) Sternum VIII (ventral surface); (1327) Genitalia in dorsal view; (1328) Genitalia in lateral view. FIGURE 1329. Collecting localities of Pison nigellum Krombein.

FIGURES 1338-1343 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1338-1343. Pison oakleyi Krombein. (1338) Female clypeus and mandibles; (1339) Male clypeus and mandible; (1340) Left forewing of female; male: (1341) Sternum VIII (ventral surface); (1342) Genitalia in dorsal view; (1343) Genitalia in lateral view.

FIGURES 1356-1360 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1356-1360. Pison reichingeri Kohl. (1356) Propodeal dorsum of female; (1357) Female sternum II; male: (1358) Sternum VIII (ventral surface); (1359) Genitalia in dorsal view; (1360) Genitalia in lateral view.

FIGURES 1366-1368. Pison suspiciosum F in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1366-1368. Pison suspiciosum F. Smith, male. (1366) Sternum VIII (ventral surface); (1367) Genitalia in dorsal view; (1368) Genitalia in lateral view.

FIGURES 1345-1348 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1345-1348. Pison ponape Krombein, female. (1345) Upper frons; (1346) Head in dorsal view; (1347) Propodeal dorsum; (1348) Propodeal posterior surface. FIGURE 1349. Collecting localities of Pison ponape Krombein.

FIGURES 1215-1219 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1215-1219. Pison xenognathos Pulawski, sp. nov. (1215) Female clypeus and mandibles; (1216) Male clypeus and mandibles; (1217) Female mandible in lateral view (arrow shows mandibular expansion); (1218) Male mandible in lateral view (arrow shows mandibular expansion); (1219) Lower gena of female showing psammophore. margin, propleural outer margin, and forefemoral venter (Fig. 1220) with psammophores (longest setae of genal, mandibular, and forefemoral psammophores about 1.5 ×, 2.0 ×, and 1.5 ×, respectively, of greatest forefemoral width); also midfemoral venter with erect setae 1219 (Fig. 1221); lower gena impunctate and asetose between oral fossa and psammophore. Mandible tridentate apically (Fig. 1215, 1217). Length 7.5-8.1 mm; head width 2.5-2.6 mm. ♂.– Upper interocular distance equal to 0.76-0.80 × lower interocular distance; ocellocular distance equal to 0.9-1.0 × hindocellar diameter, distance between hindocelli equal to 1.2-1.4 × hindocellar diameter; eye height equal to 0.94 × distance between eye notches. Free margin of clypeal lamella broadly arcuate (Fig. 1216). Dorsal length of flagellomere I 2.0-2.1 × apical width, of flagellomere X 1.0-1.1 × apical width. Mandible bidentate apically (Fig. 1216). Sternum VIII punctate only near apex and laterally, its apical margin truncate (Fig. 1222). Genitalia: Figs. 1223, 1224. Length 6.8-7.8 mm; head width 2.3-2.5 mm.

FIGURES 1245-1246 in A Revision of the Wasp Genus Pison Jurine, 1808 of Australia and New Zealand, New Guinea, and the Pacific Islands (Hymenoptera: Crabronidae)

FIGURES 1245-1246. Pison nogorombu Pulawski. (1245) Female tergum I; (1246) Basal flagellomeres of male; (1247) Male sternum VIII (ventral surface); (1248) Male genitalia in dorsal view; (1249) Male genitalia in lateral view.