Find research datasets worth reusing

opencc-by-4.0Dec 2014View details →

PLATE IB. Natula Gorochov, 1987. (A–K), Natula matsuurai (Sugimoto, 2001): A, Head; B, Maxillary palpi; C, Second tarsal segment; D, Tympanum on fore tibia; E, Forewing lateral view showing longitudinal veins; F, Hind femur without stripe; G, Male Forewing mirror longer than wide; H, Female sub-genital plate triangular; I, Male sub-genital plate longer than wide, hind margin with a small projected median lobe; J, Hind tibial spines (3 pairs) on both sides; K, Ovipositor upcurved with a dorsal groove in JHABAR MAL, RAJENDRA NAGAR & R. SWAMINATHAN (2014) Record of Natula matsuurai Sugimoto (Orthoptera: Gryllidae: Trigonidiinae) and other sword-tailed crickets from India. Zootaxa, 3760(3): 458-462.

PLATE IB. Natula Gorochov, 1987. (A–K), Natula matsuurai (Sugimoto, 2001): A, Head; B, Maxillary palpi; C, Second tarsal segment; D, Tympanum on fore tibia; E, Forewing lateral view showing longitudinal veins; F, Hind femur without stripe; G, Male Forewing mirror longer than wide; H, Female sub-genital plate triangular; I, Male sub-genital plate longer than wide, hind margin with a small projected median lobe; J, Hind tibial spines (3 pairs) on both sides; K, Ovipositor upcurved with a dorsal groove

opencc-by-4.0May 2021View details →

Longitudinal structural MRI and behavioural data for mice prenatally exposed to maternal immune activation either early or late in gestation

Prenatal maternal immune activation (MIA) is a risk factor for neurodevelopmental disorders. How the gestational timing of MIA-exposure differentially impacts downstream development remains unclear. The data presented here includes longitudinal structural magnetic resonance imaging (MRI) data from weaning to adulthood, and behavioural testing in adolescence and adulthood on C57BL/6 mice exposed to MIA induced by the viral mimetic, polyinosinic:polycytidylic acid (poly I:C) either early (gestational day [GD]9) or late (GD17) in gestation.   The data published here was collected and analyzed for the following publication, where more details can be found (Guma et al., 2021 https://doi.org/10.1016/j.biopsych.2021.03.017). Briefly, we found that early MIA-exposure was associated with accelerated brain volume increases in adolescence/early-adulthood that normalized in later adulthood, in regions including the striatum, hippocampus, and cingulate cortex. Similarly, alterations in anxiety-like, stereotypic, and sensorimotor gating behaviours observed in adolescence normalized in adulthood. In contrast, MIA-exposure in late gestation had less impact on anatomical and behavioural profiles.  In addition to the univariate analyses described above, we also undertook a multivariate analysis (partial least squares) to relate imaging and behavioural variables for the time of greatest alteration, i.e. adolescence/early adulthood. We further explored the molecular underpinnings of region-specific alterations in early MIA-exposed mice in adolescence using RNA sequencing (data for differentially expressed genes in the anterior cingulate cortex, dorsal hippocampus, and ventral hippocampus are available via the original publication https://doi.org/10.1016/j.biopsych.2021.03.017 for a separate cohort of adolescent mice prenatally exposed to MIA or vehicle at GD9).  In this dataset, you will find a total of 376 preprocessed structural MRIs (in MINC format) acquired at postnatal day ~21, ~38, ~60, and ~90 in mice exposed to poly I:C or vehicle control (0.9% sterile saline) at GD9 or 17. These are T1-weighted, manganese enhanced (50mg/kg 24 hours pre-scan), structural images at 100 micron isotropic resolution acquired on a 7 Tesla Bruker Biospec 70/30; matrix size of 180 x 160 x 90; 14.5 minutes, 2 averages, using 5% isoflurane for induction, 1.5% for maintenance of anesthesia during the scan. T1-weighted scans were preprocessed by stripping native coordinates, flipping left-right to maintain fidelity, denoising, correcting inhomogeneities in the bias field using the N4 algorithm, and registering in LSQ6 alignment (i.e. 6 degrees of freedom are allowed for imagine alignment: translations and rotations along x, y, and z dimensions). The demographics information for each animal is included in the demographics.csv file.  Behavioural tests were performed following the postnatal day 38 and 90 scans in all animals with a 2 day rest period. These include: open field test, marble burying test, three chambered social approach, and prepulse inhibition. The attentional set shifting task was also performed following the final behavioural test in the postnatal day 90 wave of behaviours. The data for all of these tests is presented in its own individual .csv spreadsheet and includes data for both the timepoints evaluated. Included in this data set are the structural MRIs in MINC format, the behavioural .csv data, and a readme.txt file providing further detail on the data structure and content, and on how to interpret the data column titles. DICOMS are also available for the structural MRI data, as are the raw (not-preprocessed) MINC files, available upon request to the authors.  Finally, the authors would like to acknowledge the funding bodies that supported the completion of this work including the Canadian Institute for Health Research, the Fonds de Recherche du Québec en Santé, and the Healthy Brains for Healthy Lives at McGill University.

Text-fig. 3. Scanning electron micrographs (a, b) and synchrotron radiation X-ray tomographic microscopy orthoslices (c–e) of flower of Lambertiflora elegans gen. et sp. nov. from the Early Cretaceous Puddledock locality, Virginia, USA (holotype, PP53796, Puddledock sample 082). a) Flower in lateral view showing long pedicel and overlapping elongated tepals; b) Detail of flower showing overlapping elongated tepals; note the numerous holes indicating the position of probable secretory cells; c) Flower in longitudinal section showing overlapping elongated tepals, remains of probable poorly developed stamens or staminodes and probable poorly developed carpels on the central conical gynoecial region of the receptacle (cut between orthoslices xz0510 and 0570); d) Flower in longitudinal section (comparable to c) showing overlapping tepals, poorly developed stamens or staminodes, and probable poorly developed carpels on the central conical gynoecial region of the receptacle; note the prominent cavities from secretory cells scattered through the tissues (cut between orthoslice xz0560 and 0575); e) Flower in transverse section showing overlapping tepals, poorly developed stamens or staminodes, and remains of probable poorly developed carpels (cut between orthoslices xy1160 and 1180). Scale bars = 1 mm (a), 500 µm (b–e). in Multiparted, Apocarpous Flowers From The Early Cretaceous Of Eastern North America And Portugal

Text-fig. 3. Scanning electron micrographs (a, b) and synchrotron radiation X-ray tomographic microscopy orthoslices (c–e) of flower of Lambertiflora elegans gen. et sp. nov. from the Early Cretaceous Puddledock locality, Virginia, USA (holotype, PP53796, Puddledock sample 082). a) Flower in lateral view showing long pedicel and overlapping elongated tepals; b) Detail of flower showing overlapping elongated tepals; note the numerous holes indicating the position of probable secretory cells; c) Flower in longitudinal section showing overlapping elongated tepals, remains of probable poorly developed stamens or staminodes and probable poorly developed carpels on the central conical gynoecial region of the receptacle (cut between orthoslices xz0510 and 0570); d) Flower in longitudinal section (comparable to c) showing overlapping tepals, poorly developed stamens or staminodes, and probable poorly developed carpels on the central conical gynoecial region of the receptacle; note the prominent cavities from secretory cells scattered through the tissues (cut between orthoslice xz0560 and 0575); e) Flower in transverse section showing overlapping tepals, poorly developed stamens or staminodes, and remains of probable poorly developed carpels (cut between orthoslices xy1160 and 1180). Scale bars = 1 mm (a), 500 µm (b–e).

Text-fig. 2. Synchrotron radiation X-ray tomographic microscopy volume renderings (a, b) and orthoslices (c–e) of Mugideiriflora portugallica gen. et sp. nov. from the Early Cretaceous Catefica locality, Portugal (holotype, S174254, Catefica sample 150). Yellow dots – stamens, red dots – carpels. a) Flower in lateral view showing the broad bases of the laminar tepals; b) Flower in longitudinal section showing the flat to slightly concave floral receptacle with a central conical gynoecial region (cut between orthoslices yz0800 and 1220); c) Flower in transverse section showing the numerous laminar tepals in several series and the stamens cut in the region of the poorly differentiated anthers; note cellular differences between outer (op) and inner (in) perianth parts, as well as and transverse sections of anthers, apparently with laterally to slightly dorsally placed pollen sacs (arrow heads) (cut at orthoslice xy0770); d) Flower in transverse section showing the numerous laminar tepals in several series, flattened rhomboidal stamen bases in several series, and poorly differentiated carpels (cut at orthoslice xy0820); e) Flower in transverse section showing the numerous laminar tepals in several series, flattened rhomboidal stamen bases in several series, and poorly differentiated carpels (cut at orthoslice xy0920); f) Flower in longitudinal section showing the shallowly concave floral receptacle with laminar tepals, stamens, and a central conical gynoecial region bearing poorly differentiated carpels (cut at orthoslice yz0900); g) Flower in longitudinal section perpendicular to that in (f) showing stamens and poorly differentiated carpels (cut at orthoslice xz1630). Scale bars = 1 mm (a, b), 500 µm (c–g). in Multiparted, Apocarpous Flowers From The Early Cretaceous Of Eastern North America And Portugal

Text-fig. 2. Synchrotron radiation X-ray tomographic microscopy volume renderings (a, b) and orthoslices (c–e) of Mugideiriflora portugallica gen. et sp. nov. from the Early Cretaceous Catefica locality, Portugal (holotype, S174254, Catefica sample 150). Yellow dots – stamens, red dots – carpels. a) Flower in lateral view showing the broad bases of the laminar tepals; b) Flower in longitudinal section showing the flat to slightly concave floral receptacle with a central conical gynoecial region (cut between orthoslices yz0800 and 1220); c) Flower in transverse section showing the numerous laminar tepals in several series and the stamens cut in the region of the poorly differentiated anthers; note cellular differences between outer (op) and inner (in) perianth parts, as well as and transverse sections of anthers, apparently with laterally to slightly dorsally placed pollen sacs (arrow heads) (cut at orthoslice xy0770); d) Flower in transverse section showing the numerous laminar tepals in several series, flattened rhomboidal stamen bases in several series, and poorly differentiated carpels (cut at orthoslice xy0820); e) Flower in transverse section showing the numerous laminar tepals in several series, flattened rhomboidal stamen bases in several series, and poorly differentiated carpels (cut at orthoslice xy0920); f) Flower in longitudinal section showing the shallowly concave floral receptacle with laminar tepals, stamens, and a central conical gynoecial region bearing poorly differentiated carpels (cut at orthoslice yz0900); g) Flower in longitudinal section perpendicular to that in (f) showing stamens and poorly differentiated carpels (cut at orthoslice xz1630). Scale bars = 1 mm (a, b), 500 µm (c–g).

Text-fig. 5. Scanning electron micrographs (d–g) and synchrotron radiation X-ray tomographic microscopy orthoslices (a–c) of flowers of Atlantocarpus virginiensis gen. et sp. nov. (a–d: holotype, PP43780, Puddledock sample 156), Atlantocarpus sp. from the Early Cretaceous Buarcos locality (e, f: S105025, Buarcos sample 244) and receptacle of Atlantocarpus? from the Early Cretaceous Vale de Água locality (g: S101300, Vale de Água sample 141). a) Flower in lateral view showing scar from a single bract (br), attachment scars of tepals (t) and stamens (st) on the expanded basal portion of elongated receptacle and young carpels; b) Flower in lateral view showing expanded basal portion of the elongated receptacle and young carpels; c) Flower in longitudinal section showing expanded basal portion of the elongated receptacle and young carpels; note the irregular, possibly expanded stigmatic region (arrow heads), (orthoslice yz0340); d) Flower in lateral view showing attachment scars of tepals (t) and stamens (st) on the expanded basal portion of elongated receptacle and young carpels with possible grooved stigmatic regions (arrow heads); e) Flower in lateral view showing expanded basal portion of elongated receptacle and young carpels; f) Detail of flower in (e) showing in Multiparted, Apocarpous Flowers From The Early Cretaceous Of Eastern North America And Portugal

Text-fig. 5. Scanning electron micrographs (d–g) and synchrotron radiation X-ray tomographic microscopy orthoslices (a–c) of flowers of Atlantocarpus virginiensis gen. et sp. nov. (a–d: holotype, PP43780, Puddledock sample 156), Atlantocarpus sp. from the Early Cretaceous Buarcos locality (e, f: S105025, Buarcos sample 244) and receptacle of Atlantocarpus? from the Early Cretaceous Vale de Água locality (g: S101300, Vale de Água sample 141). a) Flower in lateral view showing scar from a single bract (br), attachment scars of tepals (t) and stamens (st) on the expanded basal portion of elongated receptacle and young carpels; b) Flower in lateral view showing expanded basal portion of the elongated receptacle and young carpels; c) Flower in longitudinal section showing expanded basal portion of the elongated receptacle and young carpels; note the irregular, possibly expanded stigmatic region (arrow heads), (orthoslice yz0340); d) Flower in lateral view showing attachment scars of tepals (t) and stamens (st) on the expanded basal portion of elongated receptacle and young carpels with possible grooved stigmatic regions (arrow heads); e) Flower in lateral view showing expanded basal portion of elongated receptacle and young carpels; f) Detail of flower in (e) showing

Text-fig. 4. Synchrotron radiation X-ray tomographic microscopy orthoslices of flowers of Lambertiflora virginiense gen. et sp. nov. from the Early Cretaceous Puddledock locality, Virginia, USA (holotype, PP53796, Puddledock sample 081). White dots – tepals, yellow dots – stamens or staminodes, red dot – central conical gynoecial region. a) Flower in longitudinal section showing elongated overlapping tepals, remains of probable poorly developed stamens or staminodes and probable poorly developed carpels on the central conical gynoecial region of the receptacle (orthoslice yz0454); b) Flower in transverse section showing rhomboidal bases of 30 tepals, nine poorly developed stamens or staminodes, and the central poorly differentiated gynoecial region of the receptacle (orthoslice xy1160); c) Flower in transverse section at the level of the floral receptacle showing 30 tepals, nine of the poorly developed stamens or staminodes, and the central poorly differentiated gynoecial region of the receptacle (orthoslice xy1250). Scale bars = 250 µm (a–c). in Multiparted, Apocarpous Flowers From The Early Cretaceous Of Eastern North America And Portugal

Text-fig. 4. Synchrotron radiation X-ray tomographic microscopy orthoslices of flowers of Lambertiflora virginiense gen. et sp. nov. from the Early Cretaceous Puddledock locality, Virginia, USA (holotype, PP53796, Puddledock sample 081). White dots – tepals, yellow dots – stamens or staminodes, red dot – central conical gynoecial region. a) Flower in longitudinal section showing elongated overlapping tepals, remains of probable poorly developed stamens or staminodes and probable poorly developed carpels on the central conical gynoecial region of the receptacle (orthoslice yz0454); b) Flower in transverse section showing rhomboidal bases of 30 tepals, nine poorly developed stamens or staminodes, and the central poorly differentiated gynoecial region of the receptacle (orthoslice xy1160); c) Flower in transverse section at the level of the floral receptacle showing 30 tepals, nine of the poorly developed stamens or staminodes, and the central poorly differentiated gynoecial region of the receptacle (orthoslice xy1250). Scale bars = 250 µm (a–c).

Text-fig. 50. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of a flower and mature carpels of Reyanthus lusitanicus gen. et sp. nov.; Torres Vedras locality, Portugal. a) Holotype; multicarpellate flower showing numerous carpels surrounded by remains of the androecium and perianth; note the bract subtending the flower (arrowhead); b, c) Longitudinal sections perpendicular to section in (f) (b, SRXTM orthoslice xz0461) and (c, SRXTM cut voltex xz0540-0580) showing receptacle, carpels and remains of the androecium and perianth; note prominent cavities formed by the oil cells, subtending bract (arrow) and bract subtending fragmentary bud (arrowhead); d, e) Apical (d) and lateral (e) views of gynoecium showing laterally flattened carpels and dorsi-ventrally flattened stamen or staminode (* in e); f) Longitudinal section (SRXTM orthoslice yz0405) of gynoecium showing conical receptacle and carpels with densely spaced oil cells; g, h) Mature carpel of Reyanthus lusitanicus gen. et sp. nov. showing slightly curved form, rounded apex and base that tapers toward the attachment to the receptacle; i) Surface of carpel showing embedded oil cells; j) Transverse section through three carpels showing attachment of developing ovules near the ventral sutures and oil cells in the wall just below the surface (SRXTM cut voltex xy0770-0845). Specimens TV299-S136716 (holotype; a–f), TV299-S136717 (g–j). Scale bars 300 Μm (a–d, f–h), 150 Μm (e, j), 30 Μm (i). in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community

Text-fig. 50. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of a flower and mature carpels of Reyanthus lusitanicus gen. et sp. nov.; Torres Vedras locality, Portugal. a) Holotype; multicarpellate flower showing numerous carpels surrounded by remains of the androecium and perianth; note the bract subtending the flower (arrowhead); b, c) Longitudinal sections perpendicular to section in (f) (b, SRXTM orthoslice xz0461) and (c, SRXTM cut voltex xz0540-0580) showing receptacle, carpels and remains of the androecium and perianth; note prominent cavities formed by the oil cells, subtending bract (arrow) and bract subtending fragmentary bud (arrowhead); d, e) Apical (d) and lateral (e) views of gynoecium showing laterally flattened carpels and dorsi-ventrally flattened stamen or staminode (* in e); f) Longitudinal section (SRXTM orthoslice yz0405) of gynoecium showing conical receptacle and carpels with densely spaced oil cells; g, h) Mature carpel of Reyanthus lusitanicus gen. et sp. nov. showing slightly curved form, rounded apex and base that tapers toward the attachment to the receptacle; i) Surface of carpel showing embedded oil cells; j) Transverse section through three carpels showing attachment of developing ovules near the ventral sutures and oil cells in the wall just below the surface (SRXTM cut voltex xy0770-0845). Specimens TV299-S136716 (holotype; a–f), TV299-S136717 (g–j). Scale bars 300 Μm (a–d, f–h), 150 Μm (e, j), 30 Μm (i).

Text-fig. 24. Scanning electron microscope (SEM) images (a, c, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images (b, e) of fruits and seeds of Serialis antiqua (a, b) and Serialis parva (c–e); Torres Vedras locality, Portugal. a, b) Serialis antiqua lateral view of fruit showing smooth partly abraded fruit wall (a) and longitudinal section (b, SRXTM orthoslice yz0862) showing six closely adhering seeds, some of which are mature with well-preserved nutritive tissue; c–e) Serialis parva lateral view of fruit showing faint ribs (c), finely pitted seed surface with undulate anticlinal walls of the exotestal cells (d), and transverse section showing two closely adhering seeds (SRXTM orthoslice xy0913, e). Specimens, TV43-S174480 (a, b), TV43-S170076 (c), TV43-S170079 (d), TV43-S174477 (e). Scale bars 300 Μm (a–c, e), 50 Μm (d). in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community

Text-fig. 24. Scanning electron microscope (SEM) images (a, c, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images (b, e) of fruits and seeds of Serialis antiqua (a, b) and Serialis parva (c–e); Torres Vedras locality, Portugal. a, b) Serialis antiqua lateral view of fruit showing smooth partly abraded fruit wall (a) and longitudinal section (b, SRXTM orthoslice yz0862) showing six closely adhering seeds, some of which are mature with well-preserved nutritive tissue; c–e) Serialis parva lateral view of fruit showing faint ribs (c), finely pitted seed surface with undulate anticlinal walls of the exotestal cells (d), and transverse section showing two closely adhering seeds (SRXTM orthoslice xy0913, e). Specimens, TV43-S174480 (a, b), TV43-S170076 (c), TV43-S170079 (d), TV43-S174477 (e). Scale bars 300 Μm (a–c, e), 50 Μm (d).

Text-fig. 12. Scanning electron microscope (SEM) images of pollen of Sergipea sp. from a group of probable fragmentary pollen sacs; Torres Vedras locality, Portugal. a) Cluster of probable fragmentary pollen sacs that yielded the pollen in this Text-figure; b, c) Pollen grains showing the robust longitudinal ribs separated by prominent areas of granular exine; note the groove along the margins of the longitudinal ribs (arrowheads); d) Pollen grain showing the granular exine flanked by two robust ribs; note the groove along the margins of the longitudinal ribs (arrowheads). Specimen, TV44-S148012 (a–d). Scale bars 150 Μm (a), 12 Μm (c), 6 Μm (b, d). in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community

Text-fig. 12. Scanning electron microscope (SEM) images of pollen of Sergipea sp. from a group of probable fragmentary pollen sacs; Torres Vedras locality, Portugal. a) Cluster of probable fragmentary pollen sacs that yielded the pollen in this Text-figure; b, c) Pollen grains showing the robust longitudinal ribs separated by prominent areas of granular exine; note the groove along the margins of the longitudinal ribs (arrowheads); d) Pollen grain showing the granular exine flanked by two robust ribs; note the groove along the margins of the longitudinal ribs (arrowheads). Specimen, TV44-S148012 (a–d). Scale bars 150 Μm (a), 12 Μm (c), 6 Μm (b, d).

Text-fig. 19. Scanning electron microscope (SEM; d, e) and synchrotron radiation X-ray tomographic microscopy (SRXTM; a–c) images of a seed of Kvacekispermum costatum sp. nov. (a–c) and Kvacekispermum sp. (d, e); Torres Vedras locality, Portugal. a, b) Isolated, abraded seeds showing densely spaced longitudinal ridges; c) Detail of abraded seed in (b) showing surface of endotesta with imprints of crystals; d) Isolated, abraded seed showing longitudinal ridges; e) Abraded seed surface showing surface of endotesta with imprints of crystals. Specimens, TV44-S174098 (holotype; a), TV38-S174099 (b, c), TV43-S174612 (d, e). Scale bars 300 Μm (a, b, d), 50 Μm (c, e). in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community

Text-fig. 19. Scanning electron microscope (SEM; d, e) and synchrotron radiation X-ray tomographic microscopy (SRXTM; a–c) images of a seed of Kvacekispermum costatum sp. nov. (a–c) and Kvacekispermum sp. (d, e); Torres Vedras locality, Portugal. a, b) Isolated, abraded seeds showing densely spaced longitudinal ridges; c) Detail of abraded seed in (b) showing surface of endotesta with imprints of crystals; d) Isolated, abraded seed showing longitudinal ridges; e) Abraded seed surface showing surface of endotesta with imprints of crystals. Specimens, TV44-S174098 (holotype; a), TV38-S174099 (b, c), TV43-S174612 (d, e). Scale bars 300 Μm (a, b, d), 50 Μm (c, e).

Text-fig. 2. Quinquala obovata gen. et sp. nov., fruits from Kisinger Lakes flora, Wyoming. a: Lateral view of fruit on long pedicel. Note the two lateral wings and pair of longitudinal grooves representing wings extending into the matrix. UF 19376-60038. b: Lateral view of fruit on long, bent pedicel. Note the perianth scar at the base of the fruit (arrow). UF 19376-60023a. c: Lateral view of fruit on long, slightly bent pedicel. Note the pair of longitudinal grooves representing wings extending into the matrix, and in Winged Fruits Of Rutaceous Affinity From The Eocene Of Western North America

Text-fig. 2. Quinquala obovata gen. et sp. nov., fruits from Kisinger Lakes flora, Wyoming. a: Lateral view of fruit on long pedicel. Note the two lateral wings and pair of longitudinal grooves representing wings extending into the matrix. UF 19376-60038. b: Lateral view of fruit on long, bent pedicel. Note the perianth scar at the base of the fruit (arrow). UF 19376-60023a. c: Lateral view of fruit on long, slightly bent pedicel. Note the pair of longitudinal grooves representing wings extending into the matrix, and

Text-fig. 17. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of a fruit of Canrightia elongata sp. nov. (a–g) and isolated Canrightia-like seeds (h–j); Torres Vedras locality, Portugal. a–c) Holotype; fruit in lateral view showing four fused tepals at the base (c, upper arrowheads) and prominent cavities in the fruit wall formed by scattered oil bodies and possible subtending bract (c, lower arrowhead); d) Transverse section (SRXTM orthoslice xy1510) through the fruit showing three locules, one with the remains of the endothelium (top left, 1), the other two (2, 3) with remains of presumed endosperm tissue; note that the locule to the right (3) is crushed; e, f) Radial longitudinal (e; SRXTM orthoslice xz1212) in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community

Text-fig. 17. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of a fruit of Canrightia elongata sp. nov. (a–g) and isolated Canrightia-like seeds (h–j); Torres Vedras locality, Portugal. a–c) Holotype; fruit in lateral view showing four fused tepals at the base (c, upper arrowheads) and prominent cavities in the fruit wall formed by scattered oil bodies and possible subtending bract (c, lower arrowhead); d) Transverse section (SRXTM orthoslice xy1510) through the fruit showing three locules, one with the remains of the endothelium (top left, 1), the other two (2, 3) with remains of presumed endosperm tissue; note that the locule to the right (3) is crushed; e, f) Radial longitudinal (e; SRXTM orthoslice xz1212)

Text-fig. 14. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of seeds of Gastonispermum antiquum sp. nov. (a–e) and possible fruits (f–h); Torres Vedras locality, Portugal. a–c) Lateral view of seeds (holotype figured in b) showing the hilar-micropylar area and the course of the raphe; note the close association of hilum (arrowhead) and micropyle; d) Detail of seed wall showing the undulating surface of the exotesta composed of cells with sinuous cell outlines; e) Longitudinal section (orthoslice xy0914) through the hilar-micropylar area showing the hilum (arrowhead), the thickening of the endotesta around the micropyle and the course of the raphe within the exotesta (arrow); f–h) Fruits containing in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community

Text-fig. 14. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of seeds of Gastonispermum antiquum sp. nov. (a–e) and possible fruits (f–h); Torres Vedras locality, Portugal. a–c) Lateral view of seeds (holotype figured in b) showing the hilar-micropylar area and the course of the raphe; note the close association of hilum (arrowhead) and micropyle; d) Detail of seed wall showing the undulating surface of the exotesta composed of cells with sinuous cell outlines; e) Longitudinal section (orthoslice xy0914) through the hilar-micropylar area showing the hilum (arrowhead), the thickening of the endotesta around the micropyle and the course of the raphe within the exotesta (arrow); f–h) Fruits containing

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Text-fig. 11. Longitudinal defleshing marks on ochre-stained tibia 98-601a-F to extract the meat of m. fibularis longus and part of m. flexor digitorum, fossilised rootlets visible on the shaft. in Consumption Of Canid Meat At The Gravettian Předmostí Site, The Czech Republic

Text-fig. 11. Longitudinal defleshing marks on ochre-stained tibia 98-601a-F to extract the meat of m. fibularis longus and part of m. flexor digitorum, fossilised rootlets visible on the shaft.

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Text-fig. 12. Lower molars of (a) Omanitherium dhofarense and (b) Moeritherium chehbeurameuri. a) stereo occlusal views of ONHM TN 2017-50, posterior lophid of an unworn right lower molar, probably m/2, from Mohammed's molar site, Dhofar, Oman, b) stereo occlusal views of MNHN 1890-14, left lower molar from "Khenchella", Algeria (note the longitudinal sulcus in the centre-line of the tooth of Moeritherium and its absence in Omanitherium). Arrows show the course of the sulcus in the molar from "Khenchella" (scale bar 10 mm). in Large Mammals From The Rupelian Of Oman - Recent Finds

Text-fig. 12. Lower molars of (a) Omanitherium dhofarense and (b) Moeritherium chehbeurameuri. a) stereo occlusal views of ONHM TN 2017-50, posterior lophid of an unworn right lower molar, probably m/2, from Mohammed's molar site, Dhofar, Oman, b) stereo occlusal views of MNHN 1890-14, left lower molar from "Khenchella", Algeria (note the longitudinal sulcus in the centre-line of the tooth of Moeritherium and its absence in Omanitherium). Arrows show the course of the sulcus in the molar from "Khenchella" (scale bar 10 mm).

Text-fig. 1. Stutzeliastrobus bohemicus (BAYER) J.KVAČEK, No. NM-F 2746, Harcov, lectotype. a – surface view of ovuliferous cone photograph, scale bar 10 mm, b – microCT isosurface of ovuliferous cone, scale bar 10 mm, c – microCT longitudinal section of ovuliferous cone with segmented seeds, scale bar 10 mm, d – microCT longitudinal section of ovuliferous cone in yellow, seeds in red, e – 3D visualised bract-scale complex bearing three seeds, adaxial view, scale bar 2.5mm, f – 3D visualised bract-scale complex bearing two seeds, lateral view (incomplete reconstruction of the scale visualises the front seed), scale bar 2.5mm. in Stutzeliastrobus Bohemicus Comb. Nov. - Basal Cupressaceae Conifer From The Cenomanian Of The Bohemian Cretaceous Basin, Central Europe

Text-fig. 1. Stutzeliastrobus bohemicus (BAYER) J.KVAČEK, No. NM-F 2746, Harcov, lectotype. a – surface view of ovuliferous cone photograph, scale bar 10 mm, b – microCT isosurface of ovuliferous cone, scale bar 10 mm, c – microCT longitudinal section of ovuliferous cone with segmented seeds, scale bar 10 mm, d – microCT longitudinal section of ovuliferous cone in yellow, seeds in red, e – 3D visualised bract-scale complex bearing three seeds, adaxial view, scale bar 2.5mm, f – 3D visualised bract-scale complex bearing two seeds, lateral view (incomplete reconstruction of the scale visualises the front seed), scale bar 2.5mm.

Text-fig. 3. Stutzeliastrobus bohemicus (BAYER) J.KVAČEK, Harcov. a – surface view of bract-scale complex (arrow) and probably aborted seed, No. NM-F 2746 (lectotype), scale bar 1 mm, b – seed with a wing (arrow) NM-F 872, scale bar 1 mm, c – microCT perpendicular section of bract scale complex showing two seeds with wings (arrows), No. NM-F 2746 (lectotype), scale bar 1 mm, d – microCT longitudinal section of bract scale complex showing three seeds, No. NM-F 2746 (lectotype), scale bar 1 mm, e – microCT longitudinal section of bract scale complex with one seed reconstructed showing micropyle (arrow), No. NM-F 2746 (lectotype), scale bar 1 mm, f – isolated seed with a fragment of wing (arrow), No. NM-F 2746 (lectotype), scale bar 1 mm. in Stutzeliastrobus Bohemicus Comb. Nov. - Basal Cupressaceae Conifer From The Cenomanian Of The Bohemian Cretaceous Basin, Central Europe

Text-fig. 3. Stutzeliastrobus bohemicus (BAYER) J.KVAČEK, Harcov. a – surface view of bract-scale complex (arrow) and probably aborted seed, No. NM-F 2746 (lectotype), scale bar 1 mm, b – seed with a wing (arrow) NM-F 872, scale bar 1 mm, c – microCT perpendicular section of bract scale complex showing two seeds with wings (arrows), No. NM-F 2746 (lectotype), scale bar 1 mm, d – microCT longitudinal section of bract scale complex showing three seeds, No. NM-F 2746 (lectotype), scale bar 1 mm, e – microCT longitudinal section of bract scale complex with one seed reconstructed showing micropyle (arrow), No. NM-F 2746 (lectotype), scale bar 1 mm, f – isolated seed with a fragment of wing (arrow), No. NM-F 2746 (lectotype), scale bar 1 mm.

Text-fig. 6. Reyispermum parvum gen. et sp. nov. seeds from the Early Cretaceous Vale de Água locality, Portugal (a, b, d, e: sample 141, c: sample 300); Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0563) through the median plane of the seed (holotype, S174178) showing the palisade-shaped cells of exotesta and collapsed inner parts of seed coat, raised exotestal tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and remains of cellular nutritive tissue. b) Longitudinal orthoslice (xz0659) through the micropylar region perpendicular to the median plane showing the exotestal tissue surrounding the transverse micropyle slit (mi). c) Longitudinal orthoslice (yz0500) through the median plane of the seed (S175095) showing raised tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and poorly preserved cells of the seed coat. d) Longitudinal orthoslice (xz0810) through middle part of seed perpendicular to the median plane (S174178) showing seed coat and remains of cellular nutritive tissue. e) Tangential and longitudinal orthoslice (xz0162) through the seed coat of holotype (S174178) showing thickened, undulate cell walls of exotesta (ex) and the short, thin-walled cells of tegmen (te) with a finely striate wrinkled surface. Scale bars = 250 µm (a, c, d); 125 µm (b, e). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal

Text-fig. 6. Reyispermum parvum gen. et sp. nov. seeds from the Early Cretaceous Vale de Água locality, Portugal (a, b, d, e: sample 141, c: sample 300); Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0563) through the median plane of the seed (holotype, S174178) showing the palisade-shaped cells of exotesta and collapsed inner parts of seed coat, raised exotestal tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and remains of cellular nutritive tissue. b) Longitudinal orthoslice (xz0659) through the micropylar region perpendicular to the median plane showing the exotestal tissue surrounding the transverse micropyle slit (mi). c) Longitudinal orthoslice (yz0500) through the median plane of the seed (S175095) showing raised tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and poorly preserved cells of the seed coat. d) Longitudinal orthoslice (xz0810) through middle part of seed perpendicular to the median plane (S174178) showing seed coat and remains of cellular nutritive tissue. e) Tangential and longitudinal orthoslice (xz0162) through the seed coat of holotype (S174178) showing thickened, undulate cell walls of exotesta (ex) and the short, thin-walled cells of tegmen (te) with a finely striate wrinkled surface. Scale bars = 250 µm (a, c, d); 125 µm (b, e).

Text-fig. 9. Lusitanispermum choffatii gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0769) through the median plane of the seed (S174345) showing expanded exotestal cells (ex, arrow head) in the hilar region and well-preserved cellular nutritive tissue with an embedded tiny embryo (asterisk markes the margin of the embryo); note well-developed mesotesta (me) on the raphal side of seed. b) Longitudinal orthoslice (yz0750) in the median plane showing detail of micropylar part of seed with well-preserved exotesta (ex) and mesotesta (me) and tiny embryo with two rudimentary cotyledons (asterisks mark the margin of the cotyledons); note numerous granular bodies in the cellular nutritive tissue that are probably the remains of protein and lipid bodies. c) Transverse orthoslice (xy0311) through seed below hilum showing the bulging exotesta (arrow heads) (S174472); note that the exotestal cells in this region have thinner walls (arrows). d) Transverse orthoslice (xy0900) through the middle of a seed showing the uneven thickening of the anticlinal walls of the exotestal cells (ex), which are thicker towards the outside and very thin towards the inside; note also mesotesta (me) and the well-preserved cellular nutritive tissue (S174472). Scale bars = 500 µm (a, d); 250 µm (b, c). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal

Text-fig. 9. Lusitanispermum choffatii gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0769) through the median plane of the seed (S174345) showing expanded exotestal cells (ex, arrow head) in the hilar region and well-preserved cellular nutritive tissue with an embedded tiny embryo (asterisk markes the margin of the embryo); note well-developed mesotesta (me) on the raphal side of seed. b) Longitudinal orthoslice (yz0750) in the median plane showing detail of micropylar part of seed with well-preserved exotesta (ex) and mesotesta (me) and tiny embryo with two rudimentary cotyledons (asterisks mark the margin of the cotyledons); note numerous granular bodies in the cellular nutritive tissue that are probably the remains of protein and lipid bodies. c) Transverse orthoslice (xy0311) through seed below hilum showing the bulging exotesta (arrow heads) (S174472); note that the exotestal cells in this region have thinner walls (arrows). d) Transverse orthoslice (xy0900) through the middle of a seed showing the uneven thickening of the anticlinal walls of the exotestal cells (ex), which are thicker towards the outside and very thin towards the inside; note also mesotesta (me) and the well-preserved cellular nutritive tissue (S174472). Scale bars = 500 µm (a, d); 250 µm (b, c).

Text-fig. 4. Pazlia hilaris gen. et sp. nov. (a, b) from the Early Cretaceous Famalicão locality (sample 025), Portugal (holotype, S175096) and Pazliopsis reyi gen. et sp. nov. (c, d) from the Early Cretaceous Torres Vedras locality (sample 038), Portugal (S174614); Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (xy0984) through the median plane of the seed showing the strongly thickened sclerenchyma under the hilar scar (hi), micropylar slit (mi) and the raphe (ra); note cells of exotesta are almost equally tall on both raphal and anti-raphal sides of the seed and gradually become shorter towards the micropyle. b) Transverse orthoslice (xy0984) through middle of seed showing the thickened cells of the exotesta; note that cells of the exotesta are almost equally tall on both raphal and anti-raphal sides. c) Longitudinal orthoslice (yz0812) through apical part of seed in the median plane showing embryo with two rudimentary cotyledons (asterisks) and surrounding nutritive tissue; note the tiny cells of embryo with central structures we interpret as the fossilized remains of nuclei. d) Detail of (4c) showing tiny embryo with two rudimentary cotyledons (asterisks); nutritive tissue immediately surrounding the embryo is interpreted as endosperm (endo); whether there is any delimitation between this inner endosperm and the outer part of the nutritive tissues is not clear. Scale bars = 250 µm (a–c); 125 µm (d). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal

Text-fig. 4. Pazlia hilaris gen. et sp. nov. (a, b) from the Early Cretaceous Famalicão locality (sample 025), Portugal (holotype, S175096) and Pazliopsis reyi gen. et sp. nov. (c, d) from the Early Cretaceous Torres Vedras locality (sample 038), Portugal (S174614); Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (xy0984) through the median plane of the seed showing the strongly thickened sclerenchyma under the hilar scar (hi), micropylar slit (mi) and the raphe (ra); note cells of exotesta are almost equally tall on both raphal and anti-raphal sides of the seed and gradually become shorter towards the micropyle. b) Transverse orthoslice (xy0984) through middle of seed showing the thickened cells of the exotesta; note that cells of the exotesta are almost equally tall on both raphal and anti-raphal sides. c) Longitudinal orthoslice (yz0812) through apical part of seed in the median plane showing embryo with two rudimentary cotyledons (asterisks) and surrounding nutritive tissue; note the tiny cells of embryo with central structures we interpret as the fossilized remains of nuclei. d) Detail of (4c) showing tiny embryo with two rudimentary cotyledons (asterisks); nutritive tissue immediately surrounding the embryo is interpreted as endosperm (endo); whether there is any delimitation between this inner endosperm and the outer part of the nutritive tissues is not clear. Scale bars = 250 µm (a–c); 125 µm (d).