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5,978 results for “Macrophage”
Dataset related to article "Poly(I:C) stimulation is superior than Imiquimod to induce the antitumoral functional profile of tumor-conditioned macrophages."
<p>Macrophage plasticity is the ability of mononuclear phagocytes to change phenotype, function, and genetic reprogramming upon encounter of specific local stimuli. In the tumor microenvironment, Tumor-Associated Macrophages (TAMs) acquire an immune-suppressive and tumor-promoting phenotype. With the aim to re-educate TAMs to antitumor effectors, in this study, we used two immunestimulatory compounds: the TLR7 agonist Imiquimod (IMQ) and the TLR3 agonist Poly(I:C). To better mimic in vitro the response of TAMs, we used Tumor-Conditioned Macrophages (TC-Mϕ) differentiated in the presence of tumor cell supernatants. Our results show that TC-Mϕ respond differently from conventional M2-polarized macrophages. Upon stimulation with IMQ, TC-Mϕ did not upregulate major histocompatibility complex (MHC II) molecules and unexpectedly expressed increased CD206. With both compounds, TC-Mϕ produced higher levels of inflammatory cytokines than M2 macrophages. IMQ and Poly(I:C) differed in the types of regulated genes and secreted mediators. Reflecting their signaling pathways, only IMQ significantly induced IL-1β and IL-6, while only Poly(I:C) stimulated CXCL10, and both upregulated CCL5. Of note, using a novel cytotoxicity assay, Poly(I:C), but not IMQ, was effective in triggering the cytotoxic activity of TC-Mϕ against cancer cells. Overall, the results demonstrate that Poly(I:C) stimulation of TC-Mϕ is superior than IMQ in terms of macrophage re-education toward antitumor effectors.</p>
Dataset related to article "Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism."
<p>BACKGROUND:</p> <p>Thyroid carcinoma includes several variants characterized by different biological and clinical features: from indolent microcarcinoma to undifferentiated and aggressive anaplastic carcinoma. Inflammation plays a critical role in thyroid tumors. Conditions predisposing to cancer, as well as oncogene activity, contribute to the construction of an inflammatory microenvironment that facilitates thyroid tumor progression. Moreover, oncogene-induced senescence, a mechanism tightly connected with inflammation, and able to restrain or promote cancer progression, is involved in thyroid cancer. The interactions between thyroid tumor cells and the microenvironment are not completely clarified.</p> <p>METHODS:</p> <p>We characterize in vitro the interplay between macrophages and senescent thyrocytes and tumor-derived cell lines, modeling early and late thyroid tumor stages, respectively. Purified peripheral blood-derived human monocytes were exposed to thyroid cell-derived conditioned medium (CM) and assessed for phenotype by flow cytometry. The factors secreted by thyroid cells and macrophages were identified by gene expression analysis and ELISA. The protumoral effect of macrophages was assessed by wound healing assay on K1 thyroid tumor cells. The expression of PTGS2 and M2 markers in thyroid tumors was investigated in publicly available datasets.</p> <p>RESULTS:</p> <p>Human monocytes exposed to CM from senescent thyrocytes and thyroid tumor cell lines undergo M2-like polarization, showing high CD206 and low MHC II markers, and upregulation of CCL17 secretion. The obtained M2-like macrophages displayed tumor-promoting activity. Among genes overexpressed in polarizing cells, we identified the prostaglandin-endoperoxide synthase enzyme (PTGS2/COX-2), which is involved in the production of prostaglandin E2 (PGE2). By using COX-2 inhibitors we demonstrated that the M2-like polarization ability of thyroid cells is related to the production of PGE2. Co-expression of PTGS2 and M2 markers is observed a significant fraction of human thyroid tumors.</p> <p>CONCLUSIONS:</p> <p>Our results demonstrate that both senescent thyrocytes and thyroid tumor cell lines trigger M2-like macrophage polarization that is related to PGE2 secretion. This suggests that the interaction with the microenvironment occurs at both early and late thyroid tumor stages, and favors tumor progression. The co-expression of PTGS2 gene and M2 markers in human thyroid carcinoma highlights the possibility to counteract tumor growth through COX-2 inhibition.</p>
Particle surfaces to study macrophage adherence, migration and clearance
<p>These datasets are used to produce the figures/graphs published in our Advanced Functional Materials (10.1002/adfm.202002630) entitiled:</p> <p>Particle surfaces to study macrophage adherence, migration and clearance</p> <p>by</p> <p>Dedy Septiadi, Aaron Lee, Miguel Spuch-Calvar, Thomas Lee Moore, Giovanni Spiaggia, Wildan Abdussalam, Laura Rodriguez-Lorenzo, Patricia Taladriz-Blanco, Barbara Rothen-Rutishauser, Alke Petri-Fink*</p> <p>Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well-understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle model, we simulate and study key contributing factors underlying cell adhesion on non-porous silica particle surfaces, migration and engulfment. Following a 24 hour incubation period, monocyte-derived macrophages and A549 epithelial cells are able to adhere and remove particles in their local vicinity through induction of adhesive pulling arise from cell traction forces and phagocytic/endocytic mechanisms, in a size-dependent manner. We observe that such particle-decorated surfaces can be used to address the influence of surface topography on cell behavior. Substrates which presented 480 nm silica particles are able to induce greater development and maturation of focal adhesions, which play an important role in cellular mechanoregulation. Moreover, under a chemotactic influence, in the presence of 30% fetal bovine serum, macrophages are able to uptake the particles and be directed to translocate along a concentration gradient, indicating that local mechanical effects do not substantially impair normal physiological functions.</p>
Primordial GATA6 macrophages function as extravascular platelets in sterile injury
<p>Most multicellular organisms have a major body cavity that harbors immune cells. In primordial species like purple sea urchins, these cells perform phagocytic functions but are also crucial in repairing injuries. In mammals, the peritoneal cavity contains large numbers of resident GATA6<sup>+</sup> macrophages, which may play a similar role. It is unclear how cavity macrophages suspended in the fluid phase (peritoneal fluid) identify and migrate towards injuries, however. Here, we show that cavity macrophages in fluid rapidly form thrombus-like structures in response to injury using primordial scavenger receptor (SRCR) domains. Aggregates of cavity macrophages physically sealed injuries and promoted rapid repair of focal lesions. In iatrogenic surgical situations, these cavity macrophages formed extensive aggregates that promoted the growth of intra-abdominal scar tissue termed peritoneal adhesions.</p>
Particle Stiffness and Surface Topography Determine Macrophage-Mediated Removal of Surface Adsorbed Particles
<p>Cellular surface recognition and behavior are driven by a host of physical and chemical features which have been exploited to influence particle–cell interactions. Mechanical and topographical cues define the physical milieu which plays an important role in defining a range of cellular activities such as material recognition, adhesion, and migration through cytoskeletal organization and signaling. In order to elucidate the effect of local mechanical and topographical features generated by the adsorption of particles to an underlying surface on primary human monocyte‐derived macrophages (MDM), a series of poly(<em>N</em>‐isopropylacrylamide) (pNIPAM) particles with differing rigidity are self‐assembled to form a defined particle‐decorated surface. Assembly of particle‐decorated surfaces is facilitated by modification of the underlying glass to possess a positive charge through functionalization using 3‐aminopropyltriethoxysilane (APTES) or coating with poly(L‐lysine) (PLL). MDMs are noted to preferentially remove particles with higher degrees of crosslinking (stiffer) than those with lower degrees of crosslinking (softer). Alterations to the surface density of particles enabled a greater area of the particle‐decorated surface to be cleared. Uniquely, the impact of particle adsorption is evinced to have a direct impact on topographical recognition of the surface, suggesting a novel approach for controllably affecting cell‐surface recognition and response.</p>
ATAC-seq Bioanalyzer profiles from iPSC-derived macrophages
<p>Agilent Bioanalyzer profiles for the iPSC-derived macrophage ATAC-seq samples. </p>
Pro-inflammatory activation of primary microglia and macrophages increases 18kDa Translocator Protein (TSPO) expression in rodents but not humans
<p>Data underpinning JCBFM paper entitled: Pro-inflammatory activation of primary microglia and macrophages increases 18kDa Translocator Protein (TSPO) expression in rodents but not humans</p>
Toy macrophage data for LEMBAS tutorial
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Recruitment of macrophages into Hot and cold KPC tumors
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Dexamethasone Acetate-Loaded PLGA NanospheresTargeting Liver Macrophages
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Raw data to "Macrophage Plasticity and Polarization Are Altered in the Experimental Model of Multiple Sclerosis"
<p>Background: Macrophages have been identified as one of the major effectors of inflammation and demyelination in both MS and its animal model, experimental autoimmune encephalomyelitis (EAE). However, the activation and heterogeneity of macrophages in MS is not fully understood.</p> <p>Results: We performed a complete immunophenotyping of M1 and M2 macrophages from EAE and control mice through polychromatic flow cytometry. We found that M1 macrophages possessed a higher proinflammatory profile in EAE compared to control mice, since they expressed higher levels of activation/co-stimulatory markers (iNOS, CD40 and CD80) (Table 1), cytokines/chemokines (IL-6, IL-12, CCL2 and CXCL10) (Table 2) and a higher expression of Toll-like receptors (TLR2) 2 and 5 (Table 3). On the contrary, M2 of EAE mice lost their M2-like phenotype by showing a decreased expression of their signature markers CD206, CD11c, CD44 and CCL22, and a concomitant upregulation of several M1 makers such as iNOS, CD40, CD80/CD86 (Table 1) and proinflammatory CCL22 (Table 2) and TLR4 and 8.</p> <p>Conclusions: Our data account for a phenotypic alteration of M1/M2 balance during MS and this can be of crucial importance not only for a better understanding of the immunopathology of this neurodegenerative disease but also to potentially develop new macrophage-centered therapeutic strategies</p>
Genome wide mRNA sequencing data in macrophages without and with CX-5461 treatment
<p class="MsoNormal"><span>CX-5461, a novel selective RNA polymerase I inhibitor, shows potential anti-inflammatory and immunosuppressive activities. However, the molecular mechanisms underlying the inhibitory effects of CX-5461 on macrophage-mediated inflammation remain to be clarified. In the present study, we attempted to identify the systemic biological processes which were modulated by CX-5461 in inflammatory macrophages. Primary peritoneal macrophages were isolated from normal Sprague Dawley rats, and primed with lipopolysaccharide or interferon-gamma. Genome-wide RNA sequencing was performed. The study suggests that limiting cell proliferation predominates in the inhibitory effects of CX-5461 on macrophage-mediated inflammation. </span></p>
Data from: Peculiar macrophagous adaptations in a new Cretaceous pliosaurid
During the Middle and Late Jurassic, pliosaurid plesiosaurs evolved gigantic body size and a series of craniodental adaptations that have been linked to the occupation of an apex predator niche. Cretaceous pliosaurids (i.e. Brachaucheninae) depart from this morphology, being slightly smaller and lacking the macrophagous adaptations seen in earlier forms. However, the fossil record of Early Cretaceous pliosaurids is poor, concealing the evolution and ecological diversity of the group. Here, we report a new pliosaurid from the Late Hauterivian (Early Cretaceous) of Russia. Phylogenetic analyses using reduced consensus methods recover it as the basalmost brachauchenine. This pliosaurid is smaller than other derived pliosaurids, has tooth alveoli clustered in pairs and possesses trihedral teeth with complex serrated carinae. Maximum-likelihood ancestral state reconstruction suggests early brachauchenines retained trihedral teeth from their ancestors, but modified this feature in a unique way, convergent with macrophagous archosaurs or sphenacodontoids. Our findings indicate that Early Cretaceous marine reptile teeth with serrated carinae cannot be unequivocally assigned to metriorhynchoid crocodylomorphs. Furthermore, they extend the known diversity of dental adaptations seen in Sauropterygia, the longest lived clade of marine tetrapods.
Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
<p>To elucidate the mechanism underlying the aberrant monocyte/macrophage development of <em>zbtb14<sup>-/- </sup></em>mutant, GFP-positive cells were isolated from either wild-type <em>Tg(mpeg1.1:eGFP)</em> or <em>zbtb14 <sup>-/-</sup> //Tg(mpeg1.1:eGFP) </em>larvae at 2dpf by FACS. mRNA was extracted from sorted cells using RNeasy Micro (Qiagen, Manchester, UK) and mRNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit for52 Illumina and sequenced under Illumina HiSeq X Ten with pair end 150bp (PE150). </p>
Chromatin accessibility QTL lead variants in macrophages stimulated with IFNg and Salmonella
<p>Lead caQTL variants from RASQUAL and FastQTL analyses.</p>
RNA-seq Data for Bacteroides fragilis Toxin Suppresses METTL3-Mediated m6A Modification in Macrophage to Promote Inflammatory Bowel Disease
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A Multi-Omics Approach Reveals Mechanisms of Nanomaterial Toxicity and Structure-Activity-Relationships in Alveolar Macrophages
<p>This deposit contains the proteomics and metabolomics data belonging to the publication with the title "A Multi-Omics Approach Reveals Mechanisms of Nanomaterial Toxicity and Structure-Activity-Relationships in Alveolar Macrophages".</p>
Flow Cytometry Data files for Settle et al. Nature Communications, B2 integrins impose a mechanical checkpoint on macrophage phagocytosis
<p>FCS Data files for all flow cytometry results for Settle et al, B2 integrins impose a mechanical checkpoint on macrophage phagocytosis, to be published in Nature Communications. </p>
Lipid-laden macrophage reference dataset (Kloosterman et al., STAR Protocols 2024)
<p>This is a reference single cell RNA sequencing dataset containing lipid-laden macrophages (LLMs) from a murine glioblastoma and HCC model (Kloosterman and Erbani et al., Cell 2024 and Ramirez, Taranto and Ando-Kuri et al., Nature Communications 2024).</p> <p>This dataset is used for comparing metabolic activity of LLMs in newly generated single cell RNA sequencing dataset to uncover the potential source of lipids for macrophages in other types of cancer. The code to integrate and analyse this dataset can be found in Kloosterman et al., STAR Protocols 2024. </p>
Data and Scripts for "Timing matters in Macrophage / CD4+ T cell interactions: An agent-based model comparing Mycobacterium tuberculosis host-pathogen interactions between latently infected and naïve individuals"
<p>This contains the data and graphing scripts necessary to recreate all figures in the paper "Timing matters in Macrophage / CD4+ T cell interactions: An agent-based model comparing Mycobacterium tuberculosis host-pathogen interactions between latently infected and naïve individuals". Supplemental Material for the paper is also provided here. Please refer to the README.md for instructions on how to use. The model can be found at: https://github.itap.purdue.edu/ElsjePienaarGroup/LTBINaiveinvitroModel/ along with the uncalibrated parameter files and scripts to run on HPCs.</p>
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.