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Fig. 7 in The plastic nervous system of Nemertodermatida
Fig. 7 Confocal laser scanning microscope images of Nemertoderma cf. westbladi. a Alpha-tubulin immunoreactivity. Overview of a roundish male mature animal with ring-shaped brain, paired lateral testes with developing sperms, seminal vesicle filled with sperm and male antrum at posterior terminal position. Note also unstained eggs between the testes. b Tyrosinated-tubulin immunoreactivity. Note numerous sensillae of the anterior end, a ring-shaped brain and innervation around the mouth. c Alpha-tubulin immunoreactivity. Prominent ventral neurite
Fig. 6 in The plastic nervous system of Nemertodermatida
Fig. 6 Confocal laser scanning microscope images of Sterreria sp. stained with phalloidin-TRITC. a Anterior end with diagonal, circular and longitudinal muscles of the body wall. Note the anterior hiatus of longitudinal muscles (arrows). b Mid-body, the body wall comprises only weak circular and strong longitudinal muscles. Arrowheads point to anastomoses between longitudinal muscles. c Posterior end of the body with longitudinal and circular musculature at the level of the adhesive plate. d Male opening on the dorsal side near the posterior end of the body
Fig. 3 in The plastic nervous system of Nemertodermatida
Fig. 3 Confocal laser scanning microscope images of Flagellophora cf. apelti. a–c FMRFamide IR pattern in the anterior end of the body and broom canal innervation. Note rather large unstained cells lining the canal and FMRFamide IR local nerve nets around the canal in b. d–g Apha-tubulin IR pattern. d Overview of the dorsal side of a specimen with broom organ, bursa, sperm packages. e Overview of the ventral side showing the mouth opening and subterminal male copulatory organ. f Broom organ, note the parallel tubulin IR strands in its proximal part and the coiled strands in the distal part. g Another optical section of the same animal, showing a wide brain neuropile enveloping the proximal part of the broom. Note the presence of numerous neurons with long processes surrounding the brain on the outside. Also note a group of IR neurons near the distal end of the broom. h 5-HT IR elements of the anterior part of the body, dorsal side on the right. Note
Fig. 2 in The plastic nervous system of Nemertodermatida
Fig. 2 Confocal laser scanning microscope images of double-stained specimens of Flagellophora cf. apelti. Double staining of alpha-tubulin IR (green) and FMRFamide IR (red) elements. a–c Sagittal optical sections through the anterior end of the body of the same specimen. Dorsal side is on the right. Note the median broom organ, passing ventrally of the statocyst, the unstained area on the right that presumably contains the brain neuropile and an aggregation of FMRFamide IR brain cells around it, mostly anterior to it. The apparent FMRF-amide staining of the epidermis is an artefact resulting from the high-intensity scans necessary due to the comparative weakness of the red fluorescence to that of alpha-tubulin; it is missing in Fig. 2d. d Anterior
Fig. 3 in The structure of the muscular and nervous systems of the female Intoshia linei (Orthonectida)
Fig. 3 Schematic drawings of musculature (a, b) and serotoninergic nervous system (c, d) in female I. linei. a, c lateral view. b, d dorsal view. an antherior nerve, cm circular muscles, cn circular nerve, dm dorsal muscle, gc ganglion cells, gp genital pore, lm lateral muscles, pn posterior nerves, vm ventral muscle
Fig. 1 in The structure of the muscular and nervous systems of the female Intoshia linei (Orthonectida)
Fig. 1 Muscle system of female I. linei. TRITC-phalloidin staining, CLSM. a General view, depth-coded image. b Lateral view. c Ventral view. d Longitudinal section at the level of longitudinal muscles. cm circular muscles, dm dorsal muscles, gp genital pore, lm lateral muscles, vm ventral muscle. Scale bar 10 μm
Fig. 15 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 15 Loligo vulgaris hatchling: histology of the head region (transversal sections; Richardson staining; see also interactive supplementary figure 5). a Overview with selected planes B-H (3D reconstruction; left side view). b Brachial crown with arms and tentacles. c Base of brachial crown near mouth opening. d Midline of eyes, radula. e Lobus opticus just behind the eyes, anterior basal lobe. f Central lobus opticus with optic commissure, pedal lobe. g Posterior lobus opticus, statocysts with statoliths. h Palliovisceral lobes just behind the lobus opticus, statocysts. Labels: a 1–4=arms 1–4, abl=anterior basal lobe, aco=anterior chamber organ, doc=dorsal optic commissure, es=esophagus, ey= eye, fu=funnel, ibl=inferior buccal lobe, le=lens, ipl=inner plexiform layer, mcl=magnocellular lobe, noai=inferior anterior oculomotoric nerve, of=olfactory organ, ofl=olfactory lobe, ol=optic lobe (medullar core), opl=outer plexiform layer, pdc=peduncle commissure, pdl= peduncle lobe, pl=pedal lobe, pvl=palliovisceral lobe, ra=radula, re=retina, sl=statolith, st=statocyst, svl=subvertical lobe, t=tentacle, tp=tentacular pocket, voc=ventral optic commissure, vtl=vertical lobe; arrowhead crossing of first-order giant axons
Fig. 14 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 14 Idiosepius notoides hatchling: histology of the head region (transversal sections; Richardson staining; see also interactive supplementary figure 4). a Overview with selected planes B-H (3D reconstruction; left side view). b Brachial crown with arms and tentacles. c Base of brachial crown near mouth opening, buccal mass. d Midline of eyes, brachial lobe. e Lobus opticus just behind eyes. f Central lobus opticus with optic commissure, anterior funnel. g Posterior lobus opticus, statocysts with statoliths. h Palliovisceral lobes just behind the lobus opticus, statocysts. Labels: a 1–4=arms 1–4, bl=brachial lobe, bm=buccal mass, es= esophagus, ey=eye, fu=funnel, ifl=inferior frontal lobe, le=lens, mbl=median basal lobe, np=pallial nerve, oc=optic commissure, of=olfactory organ, ol=optic lobe (medullar core), opl=outer plexiform layer, pdl=peduncle lobe, pl=pedal lobe, pvl=palliovisceral lobe, ra=radula, rc=radula cartilage, re=retina, sfl=superior frontal lobe, sl= statolith, st=statocyst, t=tentacle, tp=tentacular pocket
Fig. 11 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 11 Sepia officinalis hatchling: histology of the head region (transversal sections; Richardson staining; see also interactive supplementary figure 1). a Overview with selected planes B-H (3D reconstruction; left side view). b Brachial crown with arms and tentacles. c Base of brachial crown near mouth opening. d Midline of eyes, buccal mass, and anterior funnel. e Lobus opticus just behind the eyes. f Central lobus opticus with optic commissure. g Posterior lobus opticus, statocysts with statoliths. h Palliovisceral lobes just behind the lobus opticus. Labels: a 1–4= arms 1–4, abl=anterior basal lobe, aco=anterior chamber organ, asg=anterior salivary gland, bl=brachial lobe, bm=buccal mass, cc=cerebral connective, dg=digestive gland, es=esophagus, ey=eye, fu=funnel, le=lens, mbl=median basal lobe, mcl= magnocellular lobe, np=pallial nerve, oc=optic commissure, of= olfactory organ, ol=optic lobe (medullar core), opl=outer plexiform layer, pdc=peduncle commissure, pl=pedal lobe, psd= posterior salivary duct, psg=posterior salivary gland, pvl=palliovisceral lobe, ra=radula, rc=radula cartilage, re=retina, sl=statolith, st=statocyst, t=tentacle, tp=tentacular pocket, vtl=vertical lobe
Fig. 10 3D in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 10 3D reconstruction of the anterior subesophageal mass (comparative ventral views). a Sepia officinalis, b Rossia macrosoma, c Sepietta obscura, d Idiosepius notoides, e Loligo vulgaris, f Octopus vulgaris. abrl=anterior brachial lobe, bl=brachial lobe, bmc=brachiomagnocellular connective, bpc= brachio-pedal connective, bpvc= brachio-palliovisceral connective, nas=superior antorbital nerve, noa=anterior oculomotoric nerve, noai=inferior anterior oculomotoric nerve, nopa= anterior inferior ophthalmic nerve, pbrl=posterior brachial lobe, pl=pedal lobe
Fig. 8 Supraesophageal neuropils. 3D in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 8 Supraesophageal neuropils. 3D reconstruction of the central nervous system around the brachial lobe (comparative left lateral and frontal views; enlarge to 100 % in the PDF viewer; A-F different scales). a Sepia officinalis, b Rossia macrosoma, c Sepietta obscura, d Idiosepius notoides, e Loligo vulgaris, f Octopus vulgaris. Labels: 14=olfactory nerve, 23=sympathic nerve, abl=anterior basal lobe, bbrc=bucco-brachial connective, cbc=cerebro-buccal connective, cbrc=cerebro-brachial
Fig. 7 Subesophageal neuropils. 3D in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 7 Subesophageal neuropils. 3D reconstruction of the central nervous system: supraesophageal and subesophageal neuropils (comparative left lateral and frontal views; enlarge to 100 % in the PDF viewer; A-F different scales). a Sepia officinalis, b Rossia macrosoma, c Sepietta obscura, d Idiosepius notoides, e Loligo vulgaris, f Octopus vulgaris. Labels: 1=inferior antorbital nerve, 2=superior antorbital nerve, 3=collar nerve, 4=crista nerve, 5=anterior funnel nerve, 6=median funnel nerve, 7=posterior funnel nerve, 8=anterior oculomotoric nerve, 9=inferior anterior oculomotoric nerve, 10=superior anterior oculomotoric nerve, 11=posterior oculomotoric nerve, 12=inferior posterior oculomotoric
Fig. 13 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 13 Sepietta obscura hatchling: histology of the head region (transversal sections; b,c,e,h: Azan staining; d,f,g: Trichrome de Masson staining; see also interactive supplementary figure 3). a Overview with selected planes BH (3D reconstruction; left side view). b Brachial crown with arms and tentacles. c Beak and base of brachial crown. d Midline of eyes, brachial lobe. e Central lobus opticus just behind the eyes, anterior funnel. f Lobus opticus with optic commissure. g Posterior lobus opticus, statocysts with statoliths. h Stellate ganglion, gills, digestive gland. Labels: a 1–4=arms 1–4, aco= anterior chamber organ, bk=beak, bl=brachial lobe, dbl= dorsal basal lobe, dg=digestive gland, es=esophagus, ey=eye, fu=funnel, g=gill, ifl=inferior frontal lobe, le=lens, mbl=medi- an basal lobe, np=pallial nerve, oc=optic commissure, of=olfactory organ, ol=optic lobe (medullar core), opl=outer plexiform layer, pdc=peduncle commissure, pdl=peduncle lobe, pl= pedal lobe, re=retina, sbl=superior buccal lobe, sfl=superior frontal lobe, sg=stellate ganglion, sl=statolith, st=statocyst, t=tentacle, tp=tentacular pocket, vtl=vertical lobe
Fig. 4 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 4 Comparative retina histology (ventral portion; transversal sections at the level of lens centers). a Sepia officinalis (Richardson), b Rossia macrosoma (Trichrome de Masson), c Sepietta obscura (Trichrome de Masson), d Idiosepius notoides (Richardson), e Loligo vulgaris (Richardson), f Octopus vulgaris (Azan). Labels: bsm=basal membrane, ds=distal segments of photoreptors, ps=proximal segments of photoreceptors, rp=retinal plexus, scn=supporting cell nuclei, arrowheads peripherically thickened eye cartilage
Fig. 9 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 9 Comparative histology of the central nervous system II (transversal sections at the level of brachio-pedal connectives). a Sepia officinalis (Richardson), b Rossia macrosoma (Azan), c Sepietta obscura (Azan), d Idiosepius notoides (Richardson), e Loligo vulgaris (Richardson), f Octopus vulgaris (Azan). Labels: bpc=connectivum brachio- pedale, bpvc=connectivum brachio-pallioviscerale, nas=superior antorbital nerve, noa=anterior oculomotoric nerve, noai= inferior anterior oculomotoric nerve, nopa=anterior inferior ophthalmic nerve, pbrl=posterior brachial lobe
Fig. 12 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 12 Rossia macrosoma hatchling: histology of the head region (transversal sections; Trichrome de Masson staining, except f: Azan staining; see also interactive supplementary figure 2). a Overview with selected planes B-H (3D reconstruction; left side view). b Base of brachial crown with arms and tentacles. c Base of brachial crown with buccal mass. d Midline of eyes, radula. e Central lobus opticus just behind the eyes. f Central lobus opticus with optic commissure. g Posterior lobus opticus, statocysts with statoliths. h Palliovisceral lobes just at the end of the optic lobes. Labels: a 1– 4=arms 1–4, aco=anterior chamber organ, bm=buccal mass, es= esophagus, ey=eye, fu=funnel, ibl=inferior buccal lobe, ifl=inferior frontal lobe, le=lens, mcl=magnocellular lobe, oc=optic commissure, ofl= olfactory lobe, ol=optic lobe (medullar core), opl=outer plexiform layer, pdl=peduncle lobe, pl=pedal lobe, pvl=palliovisceral lobe, ra=radula, re=retina, sl= statolith, st=statocyst, t=tentacle, tp=tentacular pocket, vtl=vertical lobe, ys=yolk sac
Fig. 3 in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 3 Comparative eye histology (transversal sections through lens centers; surface renderings of left retinae—not true to scale; gray arrowhead pointing rostrally). a Sepia officinalis (Richardson), b Rossia macrosoma (Trichrome de Masson), c Sepietta obscura (Trichrome de Masson), d Idiosepius notoides (Richardson), e Loligo vulgaris (Richardson), f Octopus vulgaris (Azan). Labels: aco=anterior chamber organ, cb=ciliary body, co=cornea, ir=iris, le=lens, ol=optic lobe, on=optic nerve, re=retina, arrows: septum, black arrowheads: lids
Fig. 1 Midsagittal section through a in Comparative 3D microanatomy and histology of the eyes and central nervous systems in coleoid cephalopod hatchlings
Fig. 1 Midsagittal section through a cephalopod-coleoid central nervous system with its main neuropils (Loligo vulgaris). a Profiles of subesophageal and supraesophageal masses with neuropils (light gray) and perikaryal layers (dark gray)(compare, e.g., with Novicki et al. 1990, Fig. 14). b Digital YZ-reslice with labeled neuropils (2D). c Digital YZ-reslice with labeled neuropils (3D, same colors as in b). Labels: abl=anterior basal lobe, bl=brachial lobe, dbl=dorsal basal lobe, es=esophagus, ifl=inferior frontal lobe, mbl=median basal lobe, pcl=precommissural lobe, pdc=peduncle commissure, pl= pedal lobe, pvl=palliovisceral lobe, sfl=superior frontal lobe, svl= subvertical lobe, vtl=vertical lobe
Fig. 8 in The structure of the muscular and nervous systems of the orthonectid Rhopalura litoralis (Orthonectida) or what parasitism can do to an annelid
Fig. 8 Schematic diagram of the nervous system of males and females of Intoshia linei, Intoshia variabili, and Rhopalura littoralis
Fig. 6 in The structure of the muscular and nervous systems of the orthonectid Rhopalura litoralis (Orthonectida) or what parasitism can do to an annelid
Fig. 6 Schematic representation of the muscular and nervous systems of Rhopalura litoralis. a Lateral and b ventral views of the muscular system of a female. c Nervous system of a female, lateral view. d
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