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Innate preference hierarchies coupled with adult experience, rather than larval imprinting or transgenerational acclimation, determine host plant use in Pieris rapae

The evolution of host range drives diversification in phytophagous insects, and understanding the female oviposition choices is pivotal for understanding host specialization. One controversial mechanism for female host choice is Hopkins' host selection principle, where females are predicted to increase their preference for the host species they were feeding upon as larvae. A recent hypothesis posits that such larval imprinting is especially adaptive in combination with anticipatory transgenerational acclimation, so that females both allocate and adapt their offspring to their future host. We study the butterfly <i>Pieris rapae</i>, for which previous evidence suggests that females prefer to oviposit on host individuals of similar nitrogen content as the plant they were feeding upon as larvae, and where the offspring show higher performance on the mother's host type. We test the hypothesis that larval experience and anticipatory transgenerational effects influence female host plant acceptance (no-choice) and preference (choice) of two host plant species (<i>Barbarea vulgaris</i> and <i>Berteroa incana</i>) of varying nitrogen content. We then test the offspring performance on these hosts. We found no evidence of larval imprinting affecting female decision-making during oviposition, but that an adult female experience of egg laying in no-choice trials on the less-preferred host <i>Be. incana</i> slightly increased the <i>P. rapae</i> propensity to oviposit on <i>Be. incana</i> in subsequent choice trials. We found no transgenerational effects on female host acceptance or preference, but negative transgenerational effects on larval performance, because the offspring of <i>P. rapae</i> females that had developed on<i> Be. incana</i> as larvae grew slower on both hosts, and especially on <i>Be. incana</i>. Our results suggest that among host-species preferences are guided by hard-wired preference hierarchies linked to species-specific host traits and less affected by larval experience or transgenerational effects, which may be more important for females evaluating different host individuals of the same species.

opencc-zeroOct 2021View details →
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Fig. 1. A maximum-likelihood phylogenetic tree reconstructed using 16S in Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts

Fig. 1. A maximum-likelihood phylogenetic tree reconstructed using 16S rRNA gene sequences. GenBank or JGI accession numbers of these genomes are provided in parentheses. The tree was inferred based on the GTR+G model with 1000 bootstrap replicates and only bootstrap values above 60% are shown. Strains of five novel Limosilactobacillus species are labelled by different colours; labels of six L. reuteri subspecies are colour representing vertebrate host origin: green for rodents, red for pigs, blue for humans and orange for poultry. The tree was drawn with iTOL [54].

opennotspecifiedJan 2021View details →
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FIGURE 13 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 13. Mines of Phyllonorycter spp. from the Russian Far East. (A–B) Ph. ringoniella, host plant Malus mandshurica; (C–D) Ph. sorbicola, Prunus padus and Prunus maackii respectively; (E) Ph. strigulatella, Alnus hirsuta; (F) Phyllonorycter sp. 1, Tilia mandshurica; (G–H) Phyllonorycter sp. 6, Acer pseudosieboldianum. Indications: (m) mine; (t) epidermal tunnel; (b) blotch. Close up: (B–C, F, H) mine. Sampling locations: (A–D, F–H) PK, Gornotaezhnoe, MTS and forest, 22–26.VII.2016; (E) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 11–20.VII.2017.

opennotspecifiedAug 2019View details →
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FIGURE 15 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 15. Similarity of gracillariid faunas of the Russian regions and Japan. Gracillariid species number is given in the corresponding bar, number of shared species is in the circles. The similarity Sørensen–Dice coefficient is given in red.

opennotspecifiedAug 2019View details →
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FIGURE 12 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 12. Mines of Phyllonorycter spp. from the Russian Far East. (A) Ph. cretata, host plant Quercus mongolica; (B) Ph. ermani, B. platyphylla; (C) Ph. issikii, Tilia mandshurica; (D) Ph. japonica, Corylus mandshurica; (E) sampling location of Ph. junoniella; (F) Ph. nigristella, Quercus dentata; (G) Ph. orientalis, Acer pictum; (H) Ph. pastorella, Salix sp. Indications: (m) mine; (t) epidermal tunnel; (l) larva; (p) pupa. Close up: (B) deformation of the leaf due to mines (the view from the upper side); (C, F–H) mine, (E) the bush from that the specimens were collected. Sampling locations: (A–B, F) SO, Sakhalin Isl., Yuzhno- Sakhalinsk, Gagarin's Park, 11–20.VII.2017; (E) SO, Sakhalin Isl., Susunay mountain range, 14.VII.2017; (C–D, G–H) PK, Gornotaezhnoe, MTS and forest, 22–26.VII.2016.

opennotspecifiedAug 2019View details →
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FIGURE 8 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 8. Male genitalia of Phyllonorycter and Cameraria spp. from the Russian Far East. (A) Ph. reduncata, host plant Lonicera maackii, [23Pr-2016-male]; (B) Ph. ringoniella, Malus mandshurica, [10Prin-2016-male]; (C) Ph. similis, Quercus dentata, [8Psim-2016-male]; (D) Ph. ulmifoliella [15Pu-2016-male]; (E) Cameraria niphonica, Acer pseudosieboldianum, [1Cn-2016-male]. Sampling location: Gornotaezhnoe, Primorskii Krai, 22–26.VII.2016. Scale bars: (A–D) 200, (E) 250 µm.

opennotspecifiedAug 2019View details →
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FIGURE 4 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 4. Forewing pattern of Phyllonorycter spp. from the Russian Far East. (A) Ph. caraganella, host plant Caragana fruticosa, NK- 184-16-9A; (B) Ph. cavella, Betula dahurica, 111.1; (C) Ph. cretata, Quercus mongolica, NK-85-17-4; (D) Ph. issikii, T. mandshurica, NK596; (E) Ph. japonica, Corylus mandshurica, 36.2; (F) Ph. jozanae, Crataegus sp., 44.1; (G) Ph. kisoensis, Alnus hirsuta, 45.1; (H) Ph. pastorella, Salix sp., 49.1; (I) Ph. reduncata, Lonicera maackii, 55.3; (K) Ph. ringoniella, Malus mandshurica, NK-179-16-1A; (L) Ph. similis, Quercus dentata, 42.1; (M) Ph. ulmifoliella, 37.1. Sampling locations: (A) PK, Rakovka, 27.VII.2016; (B, D–M) ibidem, Gornotaezhnoe, 16.VII.2013 (D), 22-25.VII.2016 (B, E–M); (C) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 12.VII.2017. Scale bars 1 mm.

opennotspecifiedAug 2019View details →
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FIGURE 3 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 3. COI Neighbor-Joining tree of Gracillariidae species sampled in the Russian Far East. Each specimen is accompanied by its sequence number (BOLD "process ID"), species name, sampling location and host plant. Sampling locations are given between two vertical lines, i.e. |Skov.| Skovorodino; |Blag.| Blagoveshchensk; |MTS| Komarov Mountain-Taiga Station FEB RAS and the forest around; |Rakovka|, |Glukhovka| forest around the villages Rakovka and Glukhovka respectively; |Vlad.| Vladivostok; |Sikhote-Alin| Sikhote-Alin Mountains, national park "Zov tigra", |Y-S| Yuzhno-Sakhalinsk and the area around including Susunay mountain range (see Fig. 1 for details). Host plants: A. pseudosieb.—Acer pseudosieboldianum; * sampling done by sweep netting from the bush, whereas all other gracillariids were collected/ reared directly from their mines. Each leafminer species is supplied with its BIN number retrieved from BOLD; blue BINs correspond to known species, red—new BINs.

opennotspecifiedAug 2019View details →
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FIGURE 1 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 1. The area of study in the Russian Far East, June–July 2010–2017. Black circles indicate cities, yellow circles with the numbers 1–12 are the sampling locations: (1) AO, Skovorodino, tree plantation nearby train station; (2) AO, Blagoveshchensk, Friendship park; (3) KK, Komsomolsk-na-Amure, tree lines along the road; (4) PK, Gornotaezhnoe, MTS; (5) ibidem, forest; (6) PK, Glukhovka, forest; (7) PK, Rakovka, forest; (8) PK, Vladivostok, suburb, tree plantation; (9) PK, Sikhote-Alin Mountains, national park "Zov tigra"; (10) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, Botanical garden FEB RAS Sakhalin Branch; (11) ibidem, Gagarin park; (12) SO, Sakhalin Isl., Susunay mountain range, pass "Verblyud". In the left corner, on the map of Russia the sampled area is shaded in gray. Regions: (ChAO) Chukotsky Autonomous Okrug, (KamK) Kamchatskii Krai, (MA) Magadanskaya Oblast, (AO) Amurskaya Oblast, (KK) Khabarovskii Krai, (JAO) Jewish Autonomous Oblast, (AO) Amurskaya Oblast, (PK) Primorskii Krai, (SO) Sakhalinskaya Oblast (includes Sakhalin Island and Kuril Islands).

opennotspecifiedAug 2019View details →
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FIGURE 7 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 7. Male genitalia of Phyllonorycter spp. from the Russian Far East. (A) Phyllonorycter junoniella, genitalia slide [21-male]; (B) Ph. pastorella, host plant Salix sp., [12Pp-2016-male]; (C) Ph. populifoliella, Populus balsamifera, [NK-561- 1-male]; (D) Ph. pseudojezoniella, Acer saccharum, [20Pps-2016-male]; (E) Ph. kisoensis, Alnus hirsuta, [5Pk-2016-male]. Sampling location: (A) SO, Sakhalin Isl., 14.VII.2017; (B, D) PK, Gornotaezhnoe, MTS, 22–26.VII.2016; (C) AO, Blagoveshchensk, 27.VI.2016. Scale bars 200µm.

opennotspecifiedAug 2019View details →
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FIGURE 11 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 11. Mines and leaf shelters of Gracillaria, Callisto, Parornix and Phyllonorycter spp. from the Russian Far East. (A) Gracillaria sp., host plant Syringa amurensis; (B–C) Callisto sp. on Malus sp.; (D) Parornix ermolaevi, Corylus sieboldiana; (E–F) Ph. caraganella, Caragana fruticosa; (G–H) Ph. cavella, Betula platyphylla, (H) "green island" and the mine. Indications: (m) mine; (b) blotch part of the mine; (t) epidermal tunnel; (e) exit hole; (f–f2) folded leaf margin or leaf tip (the indexes 1 and 2 indicate the order of construction appearance); (g.i.) green island region. Close up: (B, E–G) mine. Sampling locations: (A–D, G–H) PK, Gornotaezhnoe, MTS and forest, 22–27.VII.2016; (E–F) ibidem, Glukhovka, forest, 27.VII.2016.

opennotspecifiedAug 2019View details →
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FIGURE 2 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 2. Sampling localities in the Russian Far East. (A–C) PK, Gornotaezhnoe, MTS: (A) European woody plant species zone, (B) Juglans mandshurica plantation, (C) East Asian woody plant species zone; (D) PK, 20 km west of MTS, artificial lake on the way to Glukhovka and Rakovka; (E–H) SO, Sakhalin Isl., Susunay mountain range: (E–G) pass "Verblud", sampling and camping area, (H) foothills of the Susunay mountain range, the Erman's birch groove. Personalities: (C) S. Gorokhova, (F) V. Sheiko, (H) N. Kirichenko (the photographs are published with the permission of SG, VS and NK). (PK) Primorskii Krai, (SO) Sakhalinskaya Oblast (includes Sakhalin Island and Kuril Islands).

opennotspecifiedAug 2019View details →
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FIGURE 9 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 9. Number of gracillariid species found on various plant plants in the Russian Far East in 2010–2017. In some cases, the same gracillariid species were found on more than one plant genus (for example, Gracillaria sp. on Syringa and Fraxinus, Phyllonorycter pastorella on Salix and Populus etc.), therefore the sum of all bars is more than the total number of species identified in the study.

opennotspecifiedAug 2019View details →
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FIGURE 5 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 5. Female genitalia of gracillariids sampled in the Russian Far East. (A) Micrurapteryx caraganella, host plant Caragana arborescens, Skovorodino, Amurskaya Oblast, 26.VI.2016; genitalia slide [39-female]; (B) Phyllonorycter nipponicella, Quercus mongolica, PK, Gornotaezhnoe, 23.VII.2016, [8-2016-female]. Scale bars: (A) 550, (B) 100 µm.

opennotspecifiedAug 2019View details →
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FIGURE 6 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 6. Male genitalia of Caloptilia and Phyllonorycter spp. from the Russian Far East. (A) Caloptilia gloriosa, host plant Acer pseudosieboldianum, genitalia slide [13-male]; (B) Phyllonorycter cavella, Betula dahurica, [11-2016-male]; (C) Ph. japonica, Corylus mandshurica, [4-2016-male]; (D) Ph. jozanae, Crataegus sp. [14Pj-2016-male]; (E) Ph. issikii, T. mandshurica, [NK596-male]. Sampling locations: PK, Gornotaezhnoe, (A–D) 22–25.VII.2016, (E) 21.VIII.2015. Scale bars: (A) 350, (B–E) 200 µm.

opennotspecifiedAug 2019View details →
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FIGURE 14 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 14. Mines, leaf shelters and pupation sites of Phyllonorycter, Chrysaster and Cameraria spp. from the Russian Far East. (A) Phyllonorycter sp. 7, host plant Ulmus glabra; (B–C) Phyllonorycter sp. 8, Juglans mandshurica; (D–E) Chrysaster hagicola, Lespedeza bicolor; (F–G) Cameraria niphonica, Acer pseudosieboldianum and A. caudatum subsp. ukurundense, respectively; (H) Phyllocnistis sp. 1, Salix sp. Indications: (m) mine; (b) blotch part of the mine; (t) epidermal tunnel; (l) larva; (p) pupation site. Close up: (C–G) mine; (H) pupation site. Sampling locations: (A) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 11–20.VII.2017; (B–H) PK, Gornotaezhnoe, MTS and forest, 22–26.VII.2016.

opennotspecifiedAug 2019View details →
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FIGURE 10 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

FIGURE 10. Mines, leaf shelters and pupation sites of Micrurapteryx and Caloptilia spp. from the Russian Far East. (A–B) Micrurapteryx caraganella, host plant Caragana arborescens; (C) M. gradatella, Vicia sp.; (D) Caloptilia acericola, Acer pseudosieboldianum; (E) C. alni, Alnus hirsuta; (F) C. betulicola, Betula platyphylla; (G) C. heringi, A. pictum; (H) C. stigmatella, Salix sp. Indications: (m) mine; (t) epidermal tunnel; (p) pupation site; (f) folded leaf margin or leaf tip; (r1–r2) rolled leaf margin or leaf tip (the indexes 1 and 2 indicate the order of construction appearance); *mine of Diptera. Close up: (C, G, H) mine. Sampling locations: (A–C) AO, Skovorodino, 26.VI.2016; (D) PK, Gornotaezhnoe, MTS and forest, 22–27.VII.2016; (E–H) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 11–20.VII.2017.

opennotspecifiedAug 2019View details →
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Fig. 2 in Inferring host-cleptoparasite complexes of South American Centridine bees (Hymenoptera: Apidae) using macroecological perspectives

Fig. 2 Potential distribution of Epiclopus and Centris bees considering both MaxEnt and SVM algorithms. Full red lines represent known hostcleptoparasite relationships, while the red dashed lines represent

opennotspecifiedMar 2019View details →
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Fig. 4 in Inferring host-cleptoparasite complexes of South American Centridine bees (Hymenoptera: Apidae) using macroecological perspectives

Fig. 4 Multivariate space used by all host and cleptoparasite species of South American Centridine bees. The solid and the dashed lines illustrate, respectively, 100% and 50% of the available (background) climate for each considered species in its natural range. For the inset corresponding to all species, the solid line represents 10% of the occurrence density and the

opennotspecifiedMar 2019View details →
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Data from: Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia Rolled-leaf Beetle (Coleoptera: Chrysomelidae)

To fully understand the ecology and evolution of plant-herbivore interactions, information regarding the life history of both immature and adult insect stages is essential. However, most knowledge of plant-herbivore associations is derived from observations of adults. One reason for this bias is that species identification of immature stages is usually challenging. DNA barcodes can be used to identify immature stages to the species-level. This technique compares short sequences of the appropriate DNA barcode loci (e.g., mitochondrial COI gene for insects) of an unidentified specimen to a known DNA barcode library. The accuracy of DNA-based identifications depends on the comprehensiveness of the DNA barcode library. We generated a comprehensive DNA barcode library for a community of Rolled-leaf Beetles (Coleoptera: Chrysomelidae) in a premontane tropical forest in Costa Rica. The DNA barcode COI accurately identified all beetle species included in this study. Using this DNA barcode library, we identified eggs and larvae of Cephaloleia histrionica Baly with 100% confidence. This new record of C. histrionica is unique in that this species completes its life cycle on a bromeliad, whereas most Cephaloleia species are associated with plants from the order Zingiberales. The life cycle, diet breadth, immature stages, and sexual dimorphism are described for C. histrionica.

opencc-zeroDec 2012View details →

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