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Vegetation data collected from Northeast Shark River Slough, Everglades National Park, Florida, USA, September 2006 - April 2025
This project was established in 2006 to document the pattern of abundance of key ecological indicators (e.g., surface water, soil, floc, periphyton and sawgrass) across the NESRS landscape. A total of 30 sites were established and monitored in 2006, 2007 and 2008. After the completion of 1-mile bridge in 2012, additional 10 new sites were established to observe the ecological impact of 1-mile bridge (known as Bridge & Census sites). In 2015, additional 40 sites were established along eight transects (T1-T8, known as near canal sites) in ENP marshes starting at, and roughly perpendicular to the L-29 canal. The purpose of these sites was to monitor the potential effects of Modified Water Deliveries (MWD) operations on changing nutrient concentrations and ratios in key ecological compartments due to increased downstream discharges from the L-29 canal beneath the 1-mile and 2.6-mile bridges and culverts along Tamiami Trail. Data collection is complete.
Dissolved Organic Carbon Stable Isotopes and Lignin Phenols from Everglades National Park (FCE LTER), South Florida, USA, January 2019 - ongoing
Estuarine and coastal waters are home to more than half of the world's population and provide many social and economic benefits to society. Additionally, these ecosystems are sites of intense carbon cycling and are under pressure from changing land uses and climate. DOM is functionally defined as the OM passed through a 0.7 µm borosilicate glass fiber filter (GF/F) and is primarily made up of viruses, bacteria, colloids, lignins, humic substances, organic acids, and other small organic compounds (Repeta, 2015). Approximately 50% of DOM is generally considered to be dissolved organic carbon (DOC) (Stedmon and Nelson, 2015). Thus, DOC can be used as a proxy for quantifying DOM. Whole water samples were collected monthly from three different transects representing marl-dominated (Taylor Slough), peat-dominated (Shark River Slough), and seagrass-dominated (Florida Bay) environments. The water was filtered using 0.7 µm porosity glass fiber filters, then acidified to pH 2 and stored at 4°C until processing occurred. DOC concentration and the stable isotope ratio of DOC (δ13C-DOC) was measured on an OI Analytical TOC analyzer coupled to an Agilent Delta V plus Isotope Ratio Mass Spectrometer using high-temperature combustion (Lalonde et al., 2014). Dissolved vanillyl (vanillin, acetovanillone, vanillin acid), syringyl (syringaldehyde, acetosyringone, syringic acid), cinnamyl (p-coumaric acid, ferulic acid), and 3,5-hydroxybenzoic acid lignin phenols were isolated from ~2 liters of 0.7 µm filtered, acidified (pH 2) water by solid phase extraction (SPE) using 1g PPL cartridges that were pre-conditioned using pH 2 water and methanol. SPE cartridges were extracted using methanol, then dried and redissolved in 2N NaOH. Cupric oxide oxidation and liquid-liquid ethyl acetate extraction were used to extract lignin oxidation products (Hedges and Ertel, 1982, modified by Goñi and Hedges, 1995; Louchouarn et al., 2000; Goñi and Montgomery, 2000; Benner and Kaiser, 2011). Briefly, NaO
Stable isotope values of consumers, producers, and organic matter in the Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, 2019 – ongoing
Wetland food webs have often been characterized as detrital-based ‘brown’ energy pyramids, whereas the relative role of autotrophic (‘green’) vs. microbial (‘brown’) energy sources falls along a continuum set by physical drivers, as well as autochthonous and allochthonous inputs (Moore et al. 2004; Evans-White & Halvorson 2017) that change with ecosystem development (Schmitz et al. 2006). In the Florida Coastal Everglades (FCE), metabolic imbalances, including the collapse of calcareous periphyton mats, begin with a loss of foundation species primary production and legacy organic matter (Gaiser et al. 2006). This process likely enhances heterotrophic microbial productivity (Schulte 2016) and the supply of detrital energy to consumers by changing bioavailable and recalcitrant carbon supplies (Baggett et al. 2013). A shift from complex periphyton communities to transient planktonic communities under elevated P exposure reduces habitat structure and animal refuges but increases ‘green’ energy supplies and edibility (Trexler et al. 2015; Naja et al. 2017). Multiple sites (n=9) within the FCE were selected to document changes in coastal food webs as a result of eutrophication and increasing hydrologic variability. The project began in 2019 and is currently ongoing. References: Baggett, L. P., Heck, K. L., Frankovich, T. A., Armitage, A. R., & Fourqurean, J. W. (2013). Stoichiometry, growth, and fecundity responses to nutrient enrichment by invertebrate grazers in sub-tropical turtle grass (Thalassia testudinum) meadows. Marine biology, 160, 169-180. Evans-White, M. A., and H. M. Halvorson. 2017. Comparing the Ecological Stoichiometry in Green and Brown Food Webs – A Review and Meta-analysis of Freshwater Food Webs. Frontiers in Microbiology 8:1184. Gaiser, E. E., Childers, D. L., Jones, R. D., Richards, J. H., Scinto, L. J., & Trexler, J. C. (2006). Periphyton responses to eutrophication in the Florida Everglades: cross‐system patterns of structural and compositional cha
Annual Water Quality in Everglades National Park, Florida Bay, West Florida Shelf, and Florida Keys National Marine Sanctuary, Florida, USA: 1994-2019
Annual (water year basis) geometric mean concentrations of total phosphorus (TP), soluble-reactive phosphorus (SRP), total nitrogen (TN), dissolved inorganic nitrogen (DIN; calculated as nitrate + nitrite + ammonia), chlorophyll-a (Chl-a), and total organic carbon (TOC) concentrations across Everglades National Park (ENP), Florida Keys National Marine Sanctuary (FKNMS), West Florida Shelf and Florida Bay. This dataset is composed of data from multiple sources including Florida Coastal Everglades, Florida International University Southeast Research Center (FIU SERC), South Florida Water Management District (SFWMD), and National Oceanic and Atmospheric Administration Atlantic Oceanographic and Meteorological Laboratory (NOAA AOML). All values reported less than the laboratory minimum detection limit (MDL) were set to one-half the MDL. Annual geometric mean concentrations were computed for monitoring locations with greater than five years of data and four samples per year with a minimum of one sample in the wet and dry seasons. This dataset was created to evaluate long-term spatial and temporal trends in nutrients, chlorophyll-a, and total organic carbon at the landscape scale relative to freshwater and marine ecosystems.
Water Quality Data (Rainfall-driven autosampler) from the Shark River Slough, Everglades National Park (FCE LTER), Florida, USA, June 2003 - ongoing
Water quality samples are being collected using ISCO autosamplers at all freshwater sites: SRS1a (not active), SRS1c (not active), SRS1d, SRS2, and SRS3. Rain level actuators are used at the sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when a rain event at a site exceeds the threshold of 2.5 cm per hour, the autosampler at that site collects a 1000mL sample 30 minutes later. The samples are retrieved from the site every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. Salinity values were not taken consistently from 2000 to mid-2017; those values were replaced by -9999 in the data. See also Shark River Slough precipitation data package (knb-lter-fce.1092) and Shark River Slough extensive water quality data (knb-lter-fce.1072) on the FCE LTER website's data catalog or in the EDI repository.
Florida Coastal Everglades LTER Research Sites in Everglades National Park, Florida, USA, January 2003 - ongoing
The dataset includes shapefile point locations for all current and past Florida Coastal Everglades LTER sites along the two major drainages in Everglades National Park, Shark River Slough and Taylor Slough. Timothy Russell used a GeoExplorer 3 to record GPS coordinates at all FCE LTER research sites that existed in 2003. Michael Rugge added coordinates of FCE LTER research sites recorded after 2003 to the shapefile.
Leaf area index and above ground biomass for Juncus roemerianus in the Grand Bay National Estuarine Research Reserve from 2015 to 2019
Leaf area index (LAI) and above ground biomass were measured in 16 permanent Juncus roemerianus vegetation plots located in the Grand Bay National Estuarine Research Reserve in Mississippi. Data were collected from 2015 to 2019. LAI were measured using a handheld ceptometer. Above ground biomass for each plot were collected from core samples.
Bird Abundances at the Hubbard Brook Experimental Forest (1969-present) and on three replicate plots (1986-2000) in the White Mountain National Forest
Bird abundances have been determined from timed censuses, territory maps and nest locations at the Hubbard Brook Experimental Forest from 1969 to the present. This data set includes counts of the number of adult birds (males and females) per 10 ha at HBEF (1969 - present) and on three additional plots within the White Mountain National Forest (1986 - 2000). These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
Carbon exchange responses of rehydrated and incubated biological soil crust samples from White Sands National Park in 2020-2022
This dataset contains photosynthetic light response data from biological soil crusts collected from a gypsum sand sheet at White Sands National Park, NM, USA in three different seasons. This study aims to 1) assess the carbon fixation capacity of biocrust types; 2) assess biocrust carbon fixation response under varying incubation times; 3) and understand variability in carbon fixation response in different seasons. Sample collection occurred in July 2020 (summer), September 2021 (fall), and March 2022 (winter). The biocrust types of interest were light cyanobacterial, dark cyanobacterial, Peltula lichen, Clavascidium lichen, and moss crusts. Samples were collected with the intention of taking carbon fixation measurements after different incubation periods (30 min, 2 hr, 6 hr, 12hr, or 24 hr in 2020, and 30 min, 2 hr, 6 hr, 12hr, 24 hr, or 36 hr in 2021 and 2022). For each condition (biocrust type and incubation time) there were five replicates in 2020 (total n=125) and ten replicates in 2021 and 2022 (total n=300). After collection, the intact samples were re-wetted and subjected to their respective incubation period and measured for photosynthetic response. The resulting light response curves and photosynthetic information was be used for comparing biocrust type, incubation time response differences, and seasonal variation to understand variability of biocrust carbon flux response at a single site. This data set includes the light response curve values and photosynthetic data calculated from these curves and raw LICOR output files compiled into 3 spreadsheet files. The included 2020 data is also associated with the White Sands National Park data from Jornada Study 549. This dataset accompanies the in-press article by Hoellrich et al. (2023) cited below, and the study is now complete. Hoellrich, Mikaela R., Darren K. James, David Bustos, Anthony Darrouzet-Nardi, Louis S. Santiago, and Nicole Pietrasiak. "Biocrust carbon exchange varies with crust type and time on Ch
2007 Environmental Protection Agency (EPA) National Lakes Assessment dataset plus derived data and additional spatially explicit ancillary environmental data.
Lake water quality is known to be affected by local and regional drivers, including lake physical characteristics, hydrology, landscape position, land cover, land use, geology, and climate. Here, we demonstrate the utility of hypothesis testing within the landscape limnology conceptual framework using a random forest algorithm on large, national-scale, spatially explicit dataset, the United States Environmental Protection Agency 2007 National Lakes Assessment. For 1026 lakes, we tested the relative importance of water quality drivers across spatial scales, the importance of hydrologic connectivity in mediating water quality drivers, and how the importance of both spatial scale and connectivity differ across response variables for five important in-lake water quality metrics (total phosphorus, total nitrogen, dissolved organic carbon, turbidity, and conductivity).
Geographically paired lake-reservoir dataset derived from the 2007 USA EPA National Lakes Assessment
Climate change poses a significant threat to lake and reservoir ecosystems, though the exact nature of these threats may differ between lakes and reservoirs. To assess differences between lakes and reservoirs that may influence their response to climate change, we compared catchment and waterbody attributes of 132 geographically paired lakes and reservoirs from the 2007 United States Environmental Protection Agencys National Lakes Assessment (NLA) dataset. The data include the NLA IDs of each waterbody and their elevation, catchment area, surface area, perimeter, maximum depth, residence time, Secchi disk depth, surface temperature, and bottom temperature. Residence time data was collected from estimates generated by Brooks, J.R., J.J. Gibson, S.J. Birks, M.H. Weber, K.D. Rodecap, J.L. Stoddard. 2014. Stable isotope estimates of evaporation: inflow and water residence time for lakes across the United States as a tool for national lake water quality assessments. Limnology and Oceanography 59(6):2150-2165.
Plant heights from permanent plots in a Spartina alterniflora-dominated marsh, Nelson Island, Parker River National Wildlife Refuge, Plum Island Ecosystems LTER, MA (2019-2025).
Plant heights are measured during the growing season in permanent plots at a Spartina alterniflora-dominated salt marsh on Nelson Island, Parker River National Wildlife Refuge, within the Plum Island Ecosystems (PIE) LTER site. Plant heights are converted to plant weight using an algorithm to generate a non-destructive estimate of aboveground plant biomass.
Long-Term Core Site Grasshopper Dynamics for the Sevilleta National Wildlife Refuge, New Mexico
Grasshoppers are important animals in semi-arid environments, both as herbivores and as food resources for higher level consumer animals. Grasshoppers tend to be numerous and speciose in semi-arid environments, especially desert grasslands, where they range from environmental specialists to environmental generalists. Grasshopper populations tend to change considerably from year to year, often in response to annual variation in rainfall and plant production. The purpose of this study was to monitor grasshopper species composition and abundance over large temporal and spatial dimentions which include black grama grassland, blue grama grassland, creosotebush shrubland, and pinyon/juniper woodland environments at the Sevilleta, in relation to seasonal and annual variation in precipitation and plant production. Data were collected for all individual species to provide information on community dynamics as well as population dynamics, starting in 1992 and continuing to the present. The working research hypothesis for this study was that grasshopper populations in all environments will correlate positively to seasonal and annual variation in precipitation and plant production. Spring grasshopper populations will be especially high during El Nino years, and late summer populations especially high during La Nina years.
Core Research Site Web Quadrat Data for the Net Primary Production Study at the Sevilleta National Wildlife Refuge, New Mexico
This dataset is part of a long-term study at the Sevilleta LTER measuring net primary production (NPP) across four distinct ecosystems: creosote-dominant shrubland (Site C, est. winter 1999), black grama-dominant grassland (Site G, est. winter 1999), blue grama-dominant grassland (Site B, est. winter 2002), and pinon-juniper woodland (Site P, est. winter 2003). Net primary production is a fundamental ecological variable that quantifies rates of carbon consumption and fixation. Estimates of NPP are important in understanding energy flow at a community level as well as spatial and temporal responses to a range of ecological processes. Above-ground net primary production is the change in plant biomass, represented by stems, flowers, fruit and and foliage, over time and incoporates growth as well as loss to death and decomposition. To measure this change the vegetation variables in this dataset, including species composition and the cover and height of individuals, are sampled twice yearly (spring and fall) at permanent 1m x 1m plots within each site. A third sampling at Site C is performed in the winter. The data from these plots is used to build regressions correlating biomass and volume via weights of select harvested species obtained in SEV157, "Net Primary Productivity (NPP) Weight Data." This biomass data is included in SEV182, "Seasonal Biomass and Seasonal and Annual NPP for Core Research Sites." This dataset is designated as NA-US-011 in the Global Index of Vegetation-Plot Databases (GIVD). To aid tracking of the use of databases in this index, please also reference this number when citing this data. The GIVD report for SEV129 can be found in: Biodiversity and Ecology 4 - Vegetation Databases for the 21st Century (2012) by J. Dengler et al.
Core Site Phenology Study from the Chihuahaun Desert Grassland and Shrubland at the Sevilleta National Wildlife Refuge, New Mexico
Plant phenology or life-history patterns change seasonally as plants grow, mature, flower, and produce fruit and seed. Plant phenology follows seasonal patterns, yet variation may occur due to annual differences in the timing of rainfall and ambient temperature shifts. Foliage growth, flower, fruit, and seed production are important aspects of plant population dynamics and food resource availability for animals. The purpose of this study is to assess phenological patterns of plants across a series of biotic communities that represent an environmental moisture gradient. These communities all in the Chihuahuan Desert include: creosote bush shrubland, black grama grassland, and blue grama grassland. Plant phenology is recorded for all plant species across 4 replicate 200 m transects at each of the 3 habitat sites. Plant phenology measurements are taken once every month from February through October. The first ten individuals (or ten representative individuals) of each plant species encountered along each transect are assessed for life-history status. Data from the site P and J were only collected in 2000 and 2001 and are included in this data set.
Nitrogen Fertilization Experiment (NFert): Net Primary Production Quadrat Data at the Sevilleta National Wildlife Refuge, New Mexico
This dataset is part of a long-term study at the Sevilleta LTER, begun in spring 2004, which examines how fertilization affects above-ground biomass production (ANPP) in a mixed desert-grassland. Net primary production is a fundamental ecological variable that quantifies rates of carbon consumption and fixation. Estimates of NPP are important in understanding energy flow at a community level as well as spatial and temporal responses to a range of ecological processes. Above-ground net primary production is the change in plant biomass, represented by stems, flowers, fruit and foliage, over time and incorporates growth as well as loss to death and decomposition. To measure this change the vegetation variables in this dataset, including species composition and the cover and height of individuals, are sampled twice yearly (spring and fall) at permanent 1m x 1m plots. The data from these plots is used to build regressions correlating biomass and volume via weights of select harvested species obtained in SEV157, "Net Primary Productivity (NPP) Weight Data." This biomass data is included in SEV186, "Nitrogen Fertilization Experiment (NFert): Seasonal Biomass and Seasonal and Annual NPP Data."
Burn Study Sites Quadrat Data for the Net Primary Production Study at the Sevilleta National Wildlife Refuge, New Mexico
In 2003, the U.S. Fish and Wildlife Service conducted a prescribed burn over a large part of the northeastern corner of the Sevilleta National Wildlife Refuge. Following this burn, a study was designed to look at the effect of fire on above-ground net primary productivity (ANPP) (i.e., the change in plant biomass, represented by stems, flowers, fruit and foliage, over time) within three different vegetation types: mixed grass (MG), mixed shrub (MS) and black grama (G). Forty permanent 1m x 1m plots were installed in both burned and unburned (i.e., control) sections of each habitat type. The core black grama site included in SEV129 is used as a G control site for analyses and does not appear in this dataset. The MG control site caught fire unexpectedly in the fall of 2009 and some plots were subsequently moved to the south. For details of how the fire affected plot placement, see Methods below. In spring 2010, sampling of plots 16-25 was discontinued at the MG (burned and control) and G (burned treatment only) sites, reducing the number of sampled plots to 30 at each.To measure ANPP (i.e., the change in plant biomass, represented by stems, flowers, fruit and foliage, over time), the vegetation variables in this dataset, including species composition and the cover and height of individuals, are sampled twice yearly (spring and fall) at each plot. The data from these plots is used to build regressions correlating biomass and volume via weights of select harvested species obtained in SEV157, "Net Primary Productivity (NPP) Weight Data." This biomass data is included in SEV185, "Burn Study Sites Seasonal Biomass and Seasonal and Annual NPP Data."
Plant Removal Study: Recovery of Vegetation Following Disturbance at the Sevilleta National Wildlife Refuge, New Mexico
In 1995, a removal study was initiated at the Sevilleta LTER to examine the response of vegetation following the removal of dominant species. Five sites were selected that were dominated by either blue grama (site 1), blue and black grama (site 2), black grama (site 3), black grama and creosote (site 4), or creosote (site 5). A sixth site was later added in the blue grama community along the foothills of the Los Pinos Mountains (site 6). At sites 1, 3, 5, and 6, five 3m x 4m plots had all plants of the dominant species removed; five 3m x 4m plots were controls. At site 2, 5 plots had blue grama removed, 5 plots had black grama removed, and 5 plots were controls. At site 4, 5 plots had black grama removed, 5 plots had creosote removed, and 5 plots were controls. Initial cover prior to removal was estimated by species for each plot. Grass was removed using a shovel to collect above-ground biomass and crowns just below the soil surface. Shrubs were removed using large clippers to collect above-ground biomass to the soil surface. All biomass removed was bagged, dried, and weighed. Plot maintenance or removal of the target dominant species is performed annually or as needed. Rain gauges were installed at each site and the corners of the areas containing each set of plots GPS'd. Plot corners are marked by nails and are flagged periodically to aid identification and minimize foot traffic in the plots. Each northeast nail has a metal tag with site and plot number on it. Erosion bridges (1 m long) were installed in plots 1, 3 and 5 (removals and controls) at sites 1-5. Initial measurements were made in 1996.
Below-Ground Net Primary Production (BNPP): Root Ingrowth Donuts in Chihuahuan Desert Grassland and Creosote Shrubland at the Sevilleta National Wildlife Refuge, New Mexico
In 2005, annually harvested root ingrowth donut structures were co-located with previously established mini-rhizotron tubes established at four sites on McKenzie Flats located on the east side of Sevilleta NWR: 10 replicate structures in both burned and unburned blue and black grama dominated grassland plots at Deep Well, 10 replicates each on nitrogen fertilization plots and respective control plots on McKenzie Flats(20 total), 10 replicates in creosote dominated shrubland at the Five Points Creosote Core site and in 2011, 13 structures were put in the Monsoon site. Roots and soil are harvested annually in late fall after the growing season, and structures are reestablished in situ for consecutive harvests each year. Each structure allows roots to be harvested at two depths (0-15 and 15-30 cm) to estimate root production, or below ground net primary productivity. In order to compare estimates of root production from two methods, root ingrowth donuts were collocated with mini-rhizotron tubes at all localities except for the burned grassland plot at Deep Well.
Warming-El Nino-Nitrogen Deposition Experiment (WENNDEx): Net Primary Production Quadrat Data at the Sevilleta National Wildlife Refuge, New Mexico
Humans are creating significant global environmental change, including shifts in climate, increased nitrogen (N) deposition, and the facilitation of species invasions. A multi-factorial field experiment is being performed in an arid grassland within the Sevilleta National Wildlife Refuge (NWR) to simulate increased nighttime temperature, higher N deposition, and heightened El Niño frequency (which increases winter precipitation by an average of 50%). The purpose of the experiment is to better understand the potential effects of environmental change on grassland community composition and the growth of introduced creosote seeds and seedlings. The focus is on the response of three dominant species, all of which are near their range margins and thus may be particularly susceptible to environmental change. It is hypothesized that warmer summer temperatures and increased evaporation will favor growth of black grama (Bouteloua eriopoda), a desert grass, but that increased winter precipitation and/or available nitrogen will favor the growth of blue grama (Bouteloua gracilis), a shortgrass prairie species. Furthermore, it is thought that the growth and survival of introduced creosote (Larrea tridentata) seeds and seedlings will be promoted by heightened winter precipitation, N addition, and warmer nighttime temperatures. Treatment effects on limiting resources (soil moisture, nitrogen mineralization), species growth (photosynthetic rates, creosote shoot elongation), species abundance, and net primary production (NPP) are all being measured to determine the interactive effects of key global change drivers on arid grassland plant community dynamics. To measure above-ground NPP (i.e., the change in plant biomass, represented by stems, flowers, fruit and foliage, over time), the vegetation variables in this dataset, including species composition and the cover and height of individuals, are sampled twice yearly (spring and fall) at permanent 1m x 1m plots. The data from these pl
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.