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245 results for “rust”
An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
<p>The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with novel disease-resistance genes. Here, we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene <em>Lr9</em>, which was introduced into bread wheat from the wild grass species <em>Aegilops</em> <em>umbellulata</em>. We establish that <em>Lr9</em> encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat <em>Lr9</em> introgression line and the putative <em>Ae</em>. <em>umbellulata</em> <em>Lr9</em> donor enabled us to assemble the ~28.4-Mb <em>Lr9</em> translocation and to identify the translocation breakpoint. We likewise cloned <em>Lr58</em>, which was reportedly introgressed from <em>Ae</em>. <em>triuncialis</em>, but has an identical coding sequence compared to <em>Lr9</em>. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.</p>
Fig. 7 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 7. Effect of CFG treatment on colony growth of the causative agent of wheat Fusarium head blight, Fusarium graminearum. A) Structures tested for anti-fungal activity CFG; 1-O-feruloylglycerol (FG); coumaric acid (CA); ferulic acid (FA). B) Radial growth of a single plug of F. graminearum on PDA plates containing 4 mM of the indicated compound or DMSO (Ctrl). Each point represents the average and standard deviation of four replicates. Error bars indicate the significant difference (n ¼ 4) with p values calculated compared to the CFG by Student's t-test demarked as *p <0.02, **p <0.0002, ***p <0.000002. C) Representative images of F. graminearum colonies after 3 days of growth on PDA plates with DMSO (Ctrl) or 4 mM of compounds.
Fig. 6 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 6. Effect of CFG treatment on urediniospore germination in vitro. Urediniospores were plated on agar containing CFG, MeOH or water and the number of germinated urediniospores counted after 24 h incubation and compared to the total number plated. A) leaf rust Puccinia triticina race isolate MBDS; B) stripe rust Puccinia striiformis f.sp. tritici isolate C-PST-5. For both experiments, error bars represent the standard deviation of 4–5 replicates. Significance of differences was determined by oneway ANOVA with LSD. test post hoc at 5% probability level. Treatments with the same letter are not significantly different.
Fig. 4 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 4. CFG accumulations are greater in RL6058 (TcþLr34res NIL) in wheat flag leaves compared to Tc, and are depleted by infection with the rust pathogen. Accumulation of CFG in Thatcher and A) RL6058 (TcþLr34res), B) TcþLr22a and C) TcþLr67res flag leaves over time, in the presence and absence of P. triticina isolate. Error bars represent the standard error of the mean (n ¼4) of the average % TIC. Bars represent Tc (grey), RL6058 or TcþLr22 or TcþLr67 NILs as indicated (black), Tc with P. triticina infection (large spots), RL6058 or TcþLr33 or TcþLr67 NILs (as indicated) with P. triticina infection (small spots). The significance of differences were determined by multifactor ANOVA with details tabulated in Supplemental Fig. S4.
Fig. 3 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 3. Structure validation of P2 metabolite isolated from the RL6058 (TcþLr34res NIL) flag leaves. A) Multiplicity edited HSQC spectrum arising from the P2 sample, including resonance assignments according to the proposed structure, shown below, with full assignments included in Table 2. B) The annotated structure of metabolite 1-O-p-Coumaroyl-3-O-feruloylglycerol (CFG) is included below.
Fig. 5 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 5. Higher accumulation of CFG in flag leaves correlates with the presence of the Lr34res allele. A) Accumulation of CFG in Lr34 haplotypes. Twenty-two lines (Table 3) were assessed across the five haplotype groups with 5 lines tested for each of haplotypes 1–4 and 2 lines tested for haplotype 5. The median, quartiles and range of % total ion count (TIC) for the LC/MS peak corresponding to 1-O-p-Coumaroyl-3-O-feruloylglycerol (CFG) for each line (n ¼ 4) is represented, grouped by haplotype. A Kruskal-Wallis (non-parametric) analysis yielded a p-value of 0.09091. B) Accumulation of CFG in the flag leaves of five Sumai sib-pairs, grown in the field, and their two parent line 'Sumai 3' and 'Thatcher' (Table 4). Each point represents the value obtained from a different line (n ¼ 1 for each line) and an independent biological replicate, with the indicated p-value calculated by one-way ANOVA. C) Change in relative accumulations of metabolites in the Sumai Lr34þ sibs versus the Sumai Lr34- sibs, and their respective parents, grown in the fields, with Peak #'s referring to metabolites identified in Fig. 1 and Table 1. Each point represents the value obtained from a different line (n ¼ 1 for each line) and an independent biological replicate, with the significance of differences at 5% probability level determined by one-way ANOVA with LSD test post hoc. Treatments with the same letter are not significantly different.
Fig. 1 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 1. LC-UV profile of phenolic compounds detected in an extract from RL6058 (TcþLr34res NIL) flag leaves. Metabolites in the flag leaf extract were separated and characterized by LC/MS. Numbers identify peaks for which flavonoid metabolite identities are assigned based on retention time, UV-λmax and mol wt in Table 1. P1 and P2 refer to nonflavonoid compounds of unknown identity. Absorbance values represent adjusted values across the entire scanned range (210–600 nm). Inset) Comparison of the elution region from 23 to 27 min for TcþLr34res and Tc emphasizing the reduced accumulation of the compound denoted as P2 in the Tc extract, compared to the TcþLr34res extract.
Fig. 2 in A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Fig. 2. Structure elucidation by HRMS analysis of the enriched P2 metabolite obtained from RL6058 (TcþLr34res NIL) flag leaves. A) HRMS spectrum of the P2 sample isolated from RL6058 flag leaves. B) Predicted putative C22H22O8 structure with proposed fragmentation pattern matching the derived product ion spectra in the HRMS spectrum.
Management of Rust in Wheat Using IPM Principles and Alternative Products
<p>During the project Rustwatch 19 field trials in winter wheat was carried out in 9 European countries during 2 seasons collecting disease data and yield data from the use of different cultivars and fungicide treatments. </p>
Tromsø Birch Rust Allergy Study. Allergy to Birch Rust, a Possible Explanation to Seasonal Airway Allergy During Autumn?
ClinicalTrials.gov study NCT04322838. IPD Sharing: YES. Countries: 1. Publications: 11.
Norwegian Birch Rust Allergy Study
ClinicalTrials.gov study NCT05661812. IPD Sharing: YES. Countries: 1. Publications: 4.
Data from: Genetic evidence for sexual reproduction and multiple infections of Norway spruce cones by the rust fungus Thekopsora areolata
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Haplotype-based genome-wide association increases the predictability of leaf rust (Puccinia triticina) resistance in wheat
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Data from: Exploring the role of asexual multiplication in poplar rust epidemics: impact on diversity and genetic structure
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Data from: Using R-based image analysis to quantify rusts on perennial ryegrass
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Data from: Genetic structure of the poplar rust fungus Melampsora larici-populina: Evidence for isolation by distance in Europe and recent founder effects overseas
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Data from: The Fungal Endophyte Epichloë festucae var. lolii Plays a Limited Role in Mediating Crown Rust Severity in Perennial Ryegrass
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Data from: Colonization history, host distribution, anthropogenic influence and landscape features shape populations of white pine blister rust, an invasive alien tree pathogen
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An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
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Temporal dynamics and biocontrol potential of a hyperparasite on coffee leaf rust across a landscape in Arabica coffee’s native range
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.