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Can diet composition estimates using stable isotope analysis of feathers predict growth and condition in nestling mountain bluebirds (Sialia currucoides)
<p>Insectivorous birds breeding in seasonal environments provision their dependent young during periods when prey diversity and abundance vary. Consequently, the composition and nutritional value of diets parents feed to their offspring may differ within and among broods, potentially affecting the condition of nestlings. In a population of mountain bluebirds (<i>Sialia currucoides</i>), we used two methods to estimate diet composition for individual nestlings: direct observation of provisioning using video recordings at 5 and 9 days post-hatch, and stable isotopes of the δ<sup>13</sup>C and δ<sup>15</sup>N in nestling feathers and prey followed by analysis with mixing models. We determined the macronutrient content (% fat and lean mass) and estimated the metabolized energy from each type of prey. We evaluated whether different methods of estimating diet composition would produce similar results, and if the types of prey nestlings ate at one or both ages affected their morphology, growth rates, or blood ketone concentration. We found that bluebirds fed their young 5 main types of prey: beetles, cicadas, grasshoppers, insect larvae, and spiders. Both observational and mixing model estimates of diet composition indicated that larvae are traded-off with grasshoppers, and that fewer larvae are provided to nestlings as the season progresses. In evaluating how diet influences individual growth and condition, estimates from direct observations had greater explanatory power than those from mixing models, indicating that diets rich in the most energy-dense prey (greatest fat content; cicadas and larvae) were associated with larger size and higher body condition, and faster rate of mass gain and growth of tarsus. Lower value prey had more limited, specific effects on nestlings, but may still be important dietary components. While isotopic methods produced estimates of diet composition that were generally informative, when applied to explain the growth and condition of nestlings they proved less useful. </p>
Text-fig. 4. Correlations of the strata in the Urema Graben, the Cheringoma Plateau and other parts of Mozambique proposed by various authors. The positions of fossiliferous units such as the Grudja and Cheringoma formations have been reasonably stable, whereas correlations of other rock units, especially the Mazamba Formation and the volcanics, have varied a great deal. The time scale is from Gradstein et al. (2020). in Stratigraphy, Chronology And Palaeontology Of The Tertiary Rocks Of The Cheringoma Plateau, Mozambique
Text-fig. 4. Correlations of the strata in the Urema Graben, the Cheringoma Plateau and other parts of Mozambique proposed by various authors. The positions of fossiliferous units such as the Grudja and Cheringoma formations have been reasonably stable, whereas correlations of other rock units, especially the Mazamba Formation and the volcanics, have varied a great deal. The time scale is from Gradstein et al. (2020).
Dataset for publication "Effects of Y and Ho doping on microstructure evolution during oxidation of extraordinary stable Hf-B-Si-Y/Ho-C-N films up to 1500 °C" in Materials & Design 237, (2024), 112589.
<p>Dataset for publication "Effects of Y and Ho doping on microstructure evolution during oxidation of extraordinary stable Hf-B-Si-Y/Ho-C-N films up to 1500 °C" in Materials & Design Volume 237, January 2024, 112589, DOI 10.1016/j.matdes.2023.112589.</p> <p>XRD patterns, SAED patterns, EDS spectra, TEM images, HRTEM images.</p>
Stable isotopes and radiocarbon dating results on Late Pleistocene red deer and horse from the Serinyà caves (Girona, Catalonia, Spain)
<p>Table 1: Minimum (Min), maximum (Max), mean and standard-deviations (SD) of <em>δ</em><sup>13</sup>C<sub>carb</sub> and <em>δ</em><sup>18</sup>O<sub>carb</sub> values per horse and red deer tooth. Numbers in bold indicate isotopic values corresponding to peaks (summer) or troughs (winter) detected from clear sinusoidal patterns in <em>δ</em><sup>18</sup>O<sub>carb</sub> values. Numbers in bold correspond to minimum and maximum values in teeth revealing a sinusoidal pattern. LM3 and UM3 stand for lower third molar and upper third molar, respectively. LM2 stands for lower second molar. R is for the right side, L is for the left side. N: number of analyses.</p> <p>Table 2: Minimum, maximum, mean and standard-deviations of <em>δ</em><sup>13</sup>C<sub>coll</sub>, <em>δ</em><sup>15</sup>N<sub>coll</sub> for horse and red deer per technocomplex phases. na stands for not applicable. N: number of samples.</p> <p>Table A.1: Detailed isotopic results (<em>δ</em><sup>13</sup>C<sub>carb</sub> and <em>δ</em><sup>18</sup>O<sub>carb</sub>) for horse teeth with incremental sampling. Underlined numbers correspond to outlier data. Brackets are added around isotopic values that are not considered for further interpretation. R stands for the right side, L is for the left side.</p> <p>Table A.2: Detailed isotopic results (<em>δ</em><sup>13</sup>C<sub>carb</sub> and <em>δ</em><sup>18</sup>O<sub>carb</sub>) for red deer teeth with incremental sampling. Underlined numbers correspond to outlier data. Brackets are added around isotopic values that are not considered reliable for further interpretation. R is for the right side, L is for the left side.</p> <p>Table A.3: Results of elemental analysis on bone or dentine (N<sub>bone or dentine</sub>) and collagen (C<sub>coll</sub>, N<sub>coll</sub>, C:N<sub>coll</sub>) and of isotopic analysis on collagen (<em>δ</em><sup>13</sup>C<sub>coll</sub>, <em>δ</em><sup>15</sup>N<sub>coll</sub>) from horse (<em>Equus ferus</em>), red deer (<em>Cervus elaphus</em>) at the Serinyà caves. Underlined numbers correspond to outlier data. Brackets are added around isotopic values that are not considered reliable for further interpretation. R is for the right side, L is for the left side. * species determination confirmed by ZooMS analysis. Tüb. stands for Tübingen and NU for National University.</p> <p>Table A.4: Results of elemental analysis on bone or dentine (N<sub>bone or dentine</sub>) and collagen (C<sub>coll</sub>, N<sub>coll</sub>, C:N<sub>coll</sub>) and of isotopic analysis on collagen (<em>δ</em><sup>13</sup>C<sub>coll</sub>, <em>δ</em><sup>15</sup>N<sub>coll</sub>) from large bovids (<em>Bos/Bison</em>) and muskox (<em>Ovibos moschatus</em>) at the Serinyà caves. Underlined numbers correspond to outlier data. Brackets are added around isotopic values that are not considered reliable for further interpretation. R is for the right side, L is for the left side. * species determination confirmed by ZooMS analysis. Tüb. stands for Tübingen.</p> <p>Table A.5: Results of elemental analysis on collagen (C<sub>coll</sub>, N<sub>coll</sub>, C:N<sub>coll</sub>) and of isotopic analysis on collagen (<em>δ</em><sup>13</sup>C<sub>coll</sub>,<em>δ</em><sup>15</sup>N<sub>coll</sub>) and radiocarbon dates (<sup>14</sup>C) from horse (<em>Equus ferus</em>), red deer (<em>Cervus elaphus</em>), reindeer (<em>Rangifer tarandus</em>), muskox (<em>Ovibos moschatus</em>), rabbit (<em>Oryctolagus cuniculus</em>) and human (<em>Homo sapiens</em>) at the Serinyà caves. R is for the right side, L is for the left side. * species determination confirmed by ZooMS analysis. na stands for not applicable, nd for not determined and ind for indetermined.</p>
Stable and radiocarbon isotope compositions of DOC and DIC in Southeast Asian drainage canals
<p>This dataset contains the stable and radiocarbon isotope compositions of dissolved organic carbon (DOC) and radiocarbon isotope compositions of dissolved inorganic carbon (DIC) produced during DOC microbial respiration and photomineralization in canal waters sampled across disturbed peatlands in West Kalimantan, Indonesia in 2022. Microbial respiration and photomineralization experiments were carried out during laboratory incubations and natural sunlight exposures of canal water samples, respectively. Significant changes in the radiocarbon isotope composition of DIC between treatment and control waters were detected using a headspace extraction technique followed by a small-carbon extraction line for radiocarbon samples. </p>
Fig. 7 in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 7 Histogram showing the frequency distribution of bulk sediment organic matter δ13C values. Red line marks the mean value (− 26.87‰). Yellow lines demarcate the standard deviation (1σ)
Fig. 6 a in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 6 a Lithostratigraphic section of Umarsar mine. b Bulk sediment organic matter δ13C stratigraphy; hyperthermal events are demarcated. c Molluscan shell carbonate c δ13C and c δ18O curve for unit 3. d Variation in TOC and e relative sea level 1 2
Fig. 5 Mollusc fossils from unit 3 a in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 5 Mollusc fossils from unit 3 a Broken shell of Pteria sp. b Partially preserved Anomia sp. c–e Nuculana sp. c within green shale, d external view of left valve, e internal view of left valve. f Articulated specimen of Caestocorbula sp. g Aphrodina sp. (white arrow) and Claibornicardia sp. (yellow arrow) in shell limestone h–j Claibornicardia sp. h internal view of left valve, i external view of left valve, j internal view of right valve. k, l Turritella sp. Scale bars 10 mm
Fig. 4 in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 4 Relative abundance of mollusc fossils in different layers shown along with the lithostratigraphic column of unit 3
Fig. 2 in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 2 Lithostratigraphic section of the Umarsar mine as revealed from the 103 no. drill-core. The four informal units of the succession are marked. Relative quantities of amber, plant matter, pyrite and glauconite in different levels are indicated
Fig. 1 in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 1 Map of the study area in Kutch, India. a Map of India. b Position of the study area. c Location of the Umarsar and Panandhro mines. Nareda village, the type locality of the Naredi Formation is also shown
Fig. 8 X in A study on benthic molluscs and stable isotopes from Kutch, western India reveals early Eocene hyperthermals and pronounced transgression during ETM2 and H2 events
Fig. 8 X-ray diffractograms of a Aphrodina sp. b Claibornicardia sp. and c Caestocorbula sp. Aragonite (Ara) is detected in the composition of all the analysed bivalve genera. Blue lines indicate the obtained curves of the respective samples and green lines indicate the standard d-spacings of aragonite (based on Profex 5.0.2 software)
Fig. 1 in Stable isotopes provide evidence of a trophic shift in the lesser spotted dogfish Scyliorhinus canicula from the Central Tyrrhenian Sea Abstract
Fig. 1: Fishing area in the Tyrrhenian Sea (black triangle) where specimens of S. canicula were obtained during 2017-2018.
Data for the paper: The Role of Glycerol in Manufacturing Freeze-Dried Chitosan and Cellulose Foams for Mechanically Stable Scaffolds in Skin Tissue Engineering
<p>The Dataset contains all the data, described in the article "<strong>The Role of Glycerol in Manufacturing Freeze-Dried Chitosan </strong><br><strong>and Cellulose Foams for Mechanically Stable Scaffolds in Skin Tissue Engineering</strong>."</p> <p><strong><em>Abstract</em></strong><br>Various strategies have extensively explored enhancing the physical and biological properties of chitosan and cellulose scaffolds for skin tissue engineering. This study presents a straightforward method involving the addition of glycerol into highly porous structures of two polysaccharide complexes: chitosan/carboxymethyl cellulose (Chit/CMC) and chitosan/oxidized cellulose (Chit/OC); during a one-step freeze-drying process. Adding glycerol, especially to Chit/CMC, significantly increased stability, prevented degradation, and improved mechanical strength by nearly 50%. Importantly, after 21 days of incubation in enzymatic medium Chit/CMC scaffold has almost completely decomposed, while foams reinforced with glycerol exhibited only 40% mass loss. It is possible due to differences in multivalent cations and polymer chain contraction, resulting in varied hydrogen bonding <br>and, consequently, distinct physicochemical outcomes. Additionally, the scaffolds with glycerol improved the cellular activities resulting in over 40% higher proliferation of fibroblast after 21 days of incubation. It was achieved by imparting water resistance to the highly absorbent material and aiding in achieving a balance between hydrophilic and hydrophobic properties. This study clearly indicates the possible elimination of additional crosslinkers and multiple fabrication steps that can reduce the cost of scaffold production for skin tissue engineering applications while tailoring mechanical strength and degradation.</p> <p><strong>Figure 2.</strong> Morphology. SEM micrographs of the internal structure of the freeze-dried scaffolds. Results of porosity analysis. The methodology and data are described in the README file in the folder.</p> <p><strong>Figure 3.</strong> Mechanical test results. Representative stress-strain curves from the tensile test of all freeze-dried scaffolds, where (A)<br>– measurement performed in dry conditions, (B) – measurement performed in wet conditions. All are described in the README file in the folder.</p> <p><strong>Figure 4. </strong>Swelling behavior of all scaffolds. B – Two representative vials with a visual demonstration of swelling, samples marked with circles: Chit/CMC sample submerged in the PBS (blue circle) and Chit/CMC/Glyc sample floating on the surface (green circle). The arrows lead to photos of scaffolds taken from vials directly after swelling. C – Gel fraction analysis in aqueous solution after 24 <br>6 h. D – Time after which the water droplet is absorbed into the scaffold. E – Photographs of water droplet shape changes on Chit/CMC and Chit/CMC/Glyc scaffolds over time. All are described in the README file in the folder.</p> <p><strong>Figure 5</strong>. Fourier Transform Infrared Spectroscopy (ATR-FTIR) analysis results. Details are in the README file in the folder.</p> <p><strong>Figure 6.</strong> The FTIR spectra of eluates from degraded scaffolds collected on a microscopic glass slide. Details are in the README file in the folder.</p> <p><strong>Figure 7.</strong> The degradation studies of all scaffolds over 21 days of experiments in A – enzymatic medium. B – cell culture medium. Details are in the README file in the folder.</p> <p><strong>Figure 9. </strong>Cell experiments and toxicity analysis. Cytotoxicity of eluates taken from degraded scaffolds. B – Direct fibroblast seeding on scaffolds during 14 days of culture period. C – Direct fibroblast seeding on scaffolds during 14 days of culture period without control to better see the effect of glycerol. Details are in the README file in the folder.</p>
Fig. 3 in Mercury and stable isotopes ( N and C) as tracers during the ontogeny of Trichiurus lepturus
Fig. 3. Relationship between δ15N and δ13C in the muscle of sub-adult and adult specimens of Trichiurus lepturus. Bars represent the standard deviation.
Fig. 1 in Mercury and stable isotopes ( N and C) as tracers during the ontogeny of Trichiurus lepturus
Fig. 1. Northern Rio de Janeiro, in south-eastern Brazil. The sampling area where the Trichiurus lepturus specimens were collected is marked with a dashed polygon.
Fig. 2 in Mercury and stable isotopes ( N and C) as tracers during the ontogeny of Trichiurus lepturus
Fig. 2. Length (cm), weight (g), total mercury concentration (THg) in dry and wet weight basis and isotopic signatures (δ15N and δ13C) of sub-adult and adult specimens of Trichiurus lepturus, considering dry season, rainy season, and all sampling periods. Data is shown as a mean and standard deviation. The scale for length, weight, and THg is different for the two ontogenetic phases.
FIGURE 1 in Stable isotope (ẟ C, ẟ O) paleoecology of the late Early Miocene mammalian fauna from Buluk, Kenya
FIGURE 1. (A) Geographic map of East Africa with the location of Buluk indicated by the red star. The location of sites with comparative data included in these analyses, Moroto, Maboko, and Fort Ternan, are indicated by black circles. (B) Simplified stratigraphic column of the Bakate Formation, adapted from McDougall and Watkins (1985) and Watkins (1989).
Fig. 4 in Stable isotope evidence for trophic overlap of sympatric Mexican Lake Chapala silversides (Teleostei: Atherinopsidae: Chirostoma spp.)
Fig. 4. Canonical variate analysis (CVA) plot and deformation grids for twelve head landmarks (shown in inset photograph) of five species of Chirostoma from Lake Chapala, Mexico. Deformation grids exaggerated 3x times to better visualize shape differences. Analysis based on museum specimens from lots at Tulane University (TU), the University of Michigan Museum of Zoology (UMMZ) and Southeastern Louisiana State University (SLU): C. consocium: UMMZ 240102 (n=30); C. jordani: SLU 5031 (n=2), 5033 (n=2), 6678 (n=2); C. labarcae: SLU 6679 (n=23), TU 31966 (n=15); C. promelas: TU 31987 (n=1), 40843 (n=5), and C. sphyraena: SLU 5032 (n=1), SLU 6676 (n=6), 6841 (n=1). Analysis carried out using tps DIG V.2 (SUNY, Stonybrook University), Procrustes fit and data alignment by principle axes. CVA carried out using MorphoJ v105a.
Fig. 1 in Stable isotope evidence for trophic overlap of sympatric Mexican Lake Chapala silversides (Teleostei: Atherinopsidae: Chirostoma spp.)
Fig. 1. Lake Chapala in West-Central Mexico with eleven sampling sites identified (sites represented by circles were located> 1 km from the shoreline, sites represented by triangles were located <1 km from the shoreline). The Lerma – Santiago River Basin is shown as reference. The Lerma (inlet) and Santiago (outlet) rivers are identified. See methods section for a description of sites, including site 11, not shown in the image.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.