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Fig. 3 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 3. Analysis of monomeric fraction of PmSTS Wild Type and mutant proteins using (A) 12% SDS PAGE and (B) 10% Native PAGE. The SDS PAGE showed high purity of PmSTS protein were obtained for wild type and for all mutants except for PmSTSΔ24W286A and PmSTSΔ24Y390S. The native PAGE showed several species of PmSTSΔ24WT, PmSTSΔ24V466E, and PmSTSΔ24Y390S/L454G that can be resolved on a 10% native gel. However, only one species of PmSTSΔ24L454G and PmSTSΔ24L454A was resolved on a 10% native gel. Smearing bands were observed for PmSTSΔ24W286A and PmSTSΔ24Y390S on the 10% native gel, probably due to the low purity as judged by 12% SDS PAGE.
Fig. 1 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 1. Superimposition of PmSTS homology model (green) with the crystal structure of Nicotiana tabacum 5-epi-aristolochene synthase (NtEAS, PDB ID:3M01). NtEAS has a FPP ligand analog (2-trans, 6-trans)-2- fluorofarnesyl diphosphate (FPF) bound in the active site (pink) shows an RMSD of 1.4 Å over 518 aligned Cα atoms. The Mg2+ ions in the active site of NtEAS are shown in yellow spheres. PmSTS and NtEAS share ~38% sequence identify. The putative active residues of PmSTS (W286, Y390, L454 and V466) shown heavier sticks were selected for mutagenesis studies. The structure superimposition was carried out using SSM in COOT (Emsley et al., 2010). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 2 in Site-directed mutagenesis of β sesquiphellandrene synthase enhances enzyme promiscuity
Fig. 2. Size exclusion chromatography (SEC) profiles of affinity chromatography purified wild-type and mutant proteins. The chromatography was performed on a HiLoad Superdex column 200 PG. Detection was at 280 nm. (A) PmSTSΔ24 WT, (B) PmSTSΔ24 V466E, (C) PmSTSΔ24 W286A, (D) PmSTSΔ24 Y390S, (E) PmSTSΔ24L454A, (F) PmSTSΔ24L454G, and (G) PmSTSΔ24Y390S/L454G were run over a size exclusion column to verify the oligomeric status of generated proteins. Elution profiles of β-sesquiphellandrene synthase proteins are represented together with protein standard: bovine thyroglobulin (670 kDa), bovine gamma globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), vitamin B12 (1.35 kDa). The peaks eluted at ~48 mL correspond to protein aggregated during the purification process, and peaks eluted at ~80 mL correspond to the monomeric size of PmSTSΔ24 protein (65 kDa).
Bibliometric Analysis of Data Mining Techniques in Human Motion Research: Future Directions
<p>bibliometric dataset</p>
Supplementary dataset for paper Long-period directivity pulses of strong ground motion during the 2023 Mw7.8 Kahramanmaraş earthquake
<p>Supplementary dataset for paper Long-period directivity pulses of strong ground motion during the 2023 Mw7.8 Kahramanmaraş earthquake by František Čejka, Jiří Zahradník, Fatih Turhan, Efthimios Sokos, and František Gallovič</p>
Figure 1. A in Directional orientation of migration in an aseasonal explosive-breeding toad from Brazil
Figure 1. A breeding pair of Melanophrŋniscus cambaraensis (snout–vent length approximately 35 mm) at the study site.
Figure 3 in Directional orientation of migration in an aseasonal explosive-breeding toad from Brazil
Figure 3. Schematic map showing arrangement of drift fences and funnel traps around the temporary stream where Melanophrŋniscus cambaraensis form explosive breeding aggregations. The areas on both sides of the road are composed of planted Araucaria angustifolia forest.
Figure 2 in Directional orientation of migration in an aseasonal explosive-breeding toad from Brazil
Figure 2. Method of data collection at the Melanophrŋniscus cambaraensis breedingsite.Layoutof driftfencesandfunneltraps (a). Thebreedingsite was completelyenclosed with two plastic sheets and funnel traps facing inwardsandoutwards weredistributedaround thefences. Notedirtroad adjacent to the breeding site. Close-up showing fence and funnel trap design (b). To hold the fence tightly to the rocky substrate, we weighted the base of the fence by folding over and stapling the lower edge of the plastic sheets and filling the resulting pocket with soil and small rocks. We inserted soil and rocks next to the funnel traps to seal spaces in the fence and placed soil on the floor of the funnel to facilitate toad entry.
Figure 4 in Directional orientation of migration in an aseasonal explosive-breeding toad from Brazil
Figure 4. Circular histograms showing the frequency and mean vector for the directional orientation of pre- and post-reproductive migration in Melanophrŋniscus cambaraensis, excluding pseudoreplicate captures. The observed data were significantly different from expected under a uniform distribution for both pre- and post-reproductive migration, regardless of gender (Rao's spacing test, all P <0.001).
Transcranial Direct Current Stimulation (tDCS) for Depression in Pregnancy: A Pilot Study
ClinicalTrials.gov study NCT02116127. IPD Sharing: Not stated. Countries: 1. Publications: 106.
Assessing Functional Capacity in Directly and Remotely Monitored Home-based Settings: A Protocol for a Multinational Validation Study in Individuals With Chronic Respiratory Diseases
ClinicalTrials.gov study NCT06447831. IPD Sharing: UNDECIDED. Countries: 2. Publications: 1.
Phase II Umbrella Study Directed by Next Generation Sequencing
ClinicalTrials.gov study NCT03574402. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Geriatric Assessment Directed Trial to Evaluate Gemcitabine +/- Nab-paclitaxel in Elderly Pancreatic Cancer Patients
ClinicalTrials.gov study NCT02812992. IPD Sharing: NO. Countries: 1. Publications: 1.
Sperms Activation by Direct Electrical Shocks on Testis in Infertile Males
ClinicalTrials.gov study NCT04173052. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Decreasing Postoperative Complications by Goal-Directed Fluid Therapy During Esophageal Resection
ClinicalTrials.gov study NCT01416077. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Medical Herbs Inhibit Inflammation Directing T Cells to Kill the COVID-19 Virus (COVID)
ClinicalTrials.gov study NCT04790240. IPD Sharing: YES. Countries: 1. Publications: 8.
Transcranial Direct Current Stimulation in Acute Ischemic Stroke Treatment
ClinicalTrials.gov study NCT04801446. IPD Sharing: NO. Countries: 1. Publications: 1.
"Real Life" Evaluation of Efficacy and Safety of Direct Antiviral Agents (DAAs) for the Treatment of Hepatitis C Virus in Egypt
ClinicalTrials.gov study NCT03510637. IPD Sharing: Not stated. Countries: 1. Publications: 3.
A Study of BCMA-directed CAR-T Cells Treatment in Subjects With r/r Multiple Myeloma
ClinicalTrials.gov study NCT04322292. IPD Sharing: NO. Countries: 1. Publications: 1.
Transcranial Direct Current Stimulation (tDCS) in Human Subjects With PTSD Receiving an Exposure-based, Behavioral Therapy
ClinicalTrials.gov study NCT05419999. IPD Sharing: YES. Countries: 1. Publications: 0.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.