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7,849 results for “Conservancy”
Fig. 5 in Hornets (Vespidae: Vespinae) of Singapore: ecology, identification, and national conservation assessment
Fig. 5. Singaporean distribution of Vespa affinis.
Nitrogen redistribution and seasonal trait fluctuation facilitate plant N conservation and ecosystem N retention
<ol> <li><span>Low available soil nitrogen (N) limits plant productivity in alpine regions, and alpine plants thus resorb and reallocate N from senescing tissues to conserve this limited N during the nongrowing season. However, the destination and extent of N redistribution during plant senescence among above- and below-ground organs, let alone other processes of translocation outside of plants and into the soil components, remain poorly understood. </span></li> <li><span>Utilizing the <sup>15</sup>N stable isotope as a tracer, we quantified N redistribution among above- and below-ground plant organs and different soil components during senescence in an alpine meadow ecosystem, and explored the relationship between <sup>15</sup>N among plant-soil N pools with seasonal fluctuations of plant functional traits.</span></li> <li><span>We found a substantial depletion of <sup>15</sup>N in fine roots (-40% ± 2.8%) and aboveground tissues (-51% ± 5.1%), and an enhanced <sup>15</sup>N storage primarily in coarse roots (+79% ± 27%) and soil organic matter (+37% ± 10%) during plant senescence. In parallel, we observed a temporal variation in plant functional traits, representing a shift from more acquisitive to more conservative strategies as the growing season ends, such as higher coarse root N and coarse root to fine root ratio. The seasonal trait variations were highly correlated with the <sup>15</sup>N retention in coarse roots and soil organic matter. Particularly, <sup>15</sup>N retention in particulate and mineral-associated organic matter increased by 30% ± 12% and 24% ± 9%, respectively, suggesting a potential pathway through which fine root and microbial mortality contribute to <sup>15</sup>N redistribution into soil N pools during senescence.</span></li> <li> <span><em>Synthesis</em>. </span><span>N redistribution and seasonal plant trait fluctuation facilitate plant N conservation and ecosystem N retention in the alpine system. This study suggests a coupled aboveground-belowground N conserving strategy that may optimize the temporal coupling between plant N demand and ecosystem N supply in N-limited alpine ecosystems. </span> </li> </ol>
Livestock guardian dogs establish a landscape of fear for wild predators: implications for the role of guardian dogs in reducing human-wildlife conflict and supporting biodiversity conservation
<ol> <li>Livestock guardian dogs (LGDs) are increasingly used to protect livestock from predators, but their effects on the distribution and behaviour of wild predators are mostly unknown. A key question is whether LGDs exclude predators from grazing land, or if predators continue to use areas with LGDs but modify their behaviour in ways that reduce impacts on livestock.</li> <li>We studied effects of LGDs (Maremma sheepdogs) on distribution and behaviour of red foxes Vulpes vulpes in north-eastern Victoria, Australia. We mapped the activity of LGDs across the study areas using GPS tracking and measured fox activity using remote cameras. We also measured risk-sensitive foraging in foxes to test if they reduced feeding time at sites regularly used by LGDs.</li> <li>Foxes occurred throughout areas occupied by LGDs, but their probability of detection was negatively related to probability of LGD presence. Foxes extracted fewer food items from experimental food stations in proportion to the intensity of local activity of LGDs. This indicates that though foxes overlapped with LGDs, they responded to risk of encountering LGDs by allocating less time to foraging. </li> <li>While LGDs do not necessarily exclude wild predators from areas used for livestock production, they can have strong effects on predator behaviour. Reduction in time allocated to foraging in areas regularly used by LGDs could lead to suppression of hunting behaviour and therefore a reduction in attacks on livestock. The flexible response of predators to LGDs should facilitate coexistence of wild predators with livestock farming, by allowing predators to continue to use areas occupied by livestock while still preventing attacks on those livestock. Our results therefore strengthen the case for use of LGDs in the conservation of predators threatened by conflict with farming. Suppression of hunting behaviour should also mean that prey species experience reduced rates of predation on farmland with LGDs. This effect could be valuable for conservation of threatened species of prey.</li> </ol>
Shiva Nataraja (after conservation), c. 1100 CE
Bronze Shiva Nataraja (Lord of the Dance), circa 1100 CE, now in the collection of the Minneapolis Institute of Art. (This scan is from after Shiva's fall 2020 conservation.) From artsmia.org: *'The Hindu god Shiva appears in several incarnations. Here, Shiva appears as Nataraja, or Lord of the Dance. He raises his left foot in a graceful dance pose. In his upper right hand, Nataraja holds a drum, the sound of which manifests creation. The flame that he holds in his upper left hand symbolizes destruction. Together, they symbolize the creation and destruction of the cosmic universe. The posture of the icon's lower left and right palms signifies protection. The foot that rests on the demon's back symbolizes the elimination of ignorance, while the raised left foot signifies salvation. A now missing ring of fire, symbolic of the simultaneous and continuous creation and destruction of the universe, would have encircled the bronze icon.'* More: https://collections.artsmia.org/art/376/shiva-nataraja-india Source: Objaverse 1.0 / Sketchfab
Addressing the Challenge of Wildlife Conservation in Urban Landscapes by Increasing Human Tolerance for Wildlife
<p>Data that supports the journal manuscript: Puri, M., Johannsen, K.L., Goode, K.O., & Pienaar, E.F. (2024) Addressing the challenge of wildlife conservation in urban landscapes by increasing human tolerance for wildlife. <em>People and Nature.</em></p>
Flying, nectar-loaded honey bees conserve water and improve heat tolerance by reducing wingbeat frequency and metabolic heat production
<p><span>Heat waves are becoming increasingly common due to climate change, making it crucial to identify and understand the capacities for insect pollinators, such as honey bees, to avoid overheating. We examined the effects of hot, dry air temperatures on the physiological and behavioral mechanisms that honey bees use to fly when carrying nectar loads, to assess how foraging is limited by overheating or desiccation. We found that flight muscle temperatures increased linearly with load mass at air temperatures of 20</span><span> or 30°C, but, remarkably, there was no change with increasing nectar loads at an air temperature of 40°C. Flying, nectar-loaded bees were able to avoid overheating at 40°C by reducing their flight metabolic rates and increasing evaporative cooling. At high body temperatures, bees apparently increase flight efficiency by lowering their wingbeat frequency and increasing stroke amplitude to compensate, reducing the need for evaporative cooling. However, even with reductions in metabolic heat production, desiccation likely limits foraging at temperatures well below bees' critical thermal maxima in hot, dry conditions.</span></p>
Bacterial zoonoses impacts to conservation of wildlife populations: A global synthesis
<p>Emerging infectious diseases have significantly increased in recent years; approximately 60% of these emerging diseases are of zoonotic origin, and of those, around 70% were identified to start with wild animals. To better understand the impacts of zoonotic diseases on wildlife, there is a need to identify the distribution and impact of zoonotic pathogens in wildlife, particular those species with threatened populations. To investigate this question, we constructed a zoonotic disease database identifying wildlife hosts for each bacterial zoonotic disease and recorded clinical signs of disease for each host-pathogen relationship if found. Species of least concern were found to have a significantly higher prevalence and richness of zoonotic bacterial diseases (n= 0.0608, x<sup>2</sup>= 5.898, p=0.01516). The taxonomic level Order was found to be the best predictor for pathogen richness, and the Artiodactyla and Carnivora contain a significantly higher pathogen richness than other vertebrate Orders. Species with the greatest pathogen richness include the wild boar (<em>Sus scrofa</em>, n=66), Northern raccoon (<em>Procyon lotor</em>, n=16) and the red fox (<em>Vulpes vulpes</em>, n=41). Our results indicate bacterial zoonoses are not likely to be a driving factor of species population declines, and future zoonotic disease surveillance efforts should target species in the Artiodactyla and Carnivora families. The wild boar (<em>Sus scrofa</em>) in particular may be a good candidate for zoonotic disease monitoring due to its high pathogen richness, wide range, and large population. Understanding the impacts and distribution of bacterial zoonoses in wildlife populations can help in planning for future wildlife management efforts, particularly in species of conservation concern and wildlife disease monitoring.</p>
Data from: mapping endemic freshwater fish richness to identify high priority areas for conservation: an ecoregion approach
<p>Freshwater ecosystems are experiencing accelerating global biodiversity loss. Thus, knowing where these unique ecosystems' species richness reaches a peak can facilitate their conservation planning. By hosting more than 290 freshwater fishes, Iran is a major freshwater fish hotspot in the Middle East. Considering the accelerating rate of biodiversity loss, there is an urgent need to identify species rich areas and understanding of the mechanisms driving biodiversity distribution. In this study, we gathered distribution records of all endemic freshwater fishes of Iran (85 species) to develop their richness map and determine the most critical drivers of their richness patterns from an ecoregion approach. We performed a generalized linear model (GLM) with quasi-Poisson distribution to identify contemporary and historical determinants of endemic freshwater fish richness. We also quantified endemic fish similarity among the 15 freshwater ecoregions of Iran. Results showed that endemic freshwater fish richness is highest in the Zagros Mountains while moderate level of richness was observed between Zagros and Alborz Mountains. High, moderate and low richness of endemic freshwater fish match with Upper Tigris & Euphrates, Namak, and Kavir & Lut Deserts ecoregions respectively. Kura - South Caspian Drainages and Caspian Highlands were the most similar ecoregions and Orumiyeh was the most unique ecoregion according to endemic fish presence. Precipitation and precipitation change velocity since the Last Glacial Maximum were the most important predictors of endemic freshwater fish richness. Areas identified to have the highest species richness have high priority for the conservation of freshwater fish in Iran, therefore, should be considered in future protected areas development.</p>
Data from: Cross ocean-basin population genetic dynamics in a pelagic top predator of high conservation concern, the oceanic whitetip shark, Carcharhinus longimanus
<p>The oceanic whitetip shark, <em>Carcharhinus longimanus</em>, is a Critically Endangered, circumtropical, and highly migratory, pelagic shark. Yet, little information exists on its population genetic dynamics to guide conservation management practice. We present a first worldwide, mitochondrial and nuclear DNA assessment of the population genetic status of this imperiled species based on sequences of the complete mitochondrial control region (n = 173) and partial ND4 gene (n = 172), and genotypes from 12 nuclear microsatellites (n = 164). Statistically significant mitochondrial and nuclear DNA population genetic differentiation was detected across all marker datasets between Western Atlantic and Indo-Pacific oceanic whitetip sharks. Additionally, our data, combined with previously published, partial (701-base pairs) mitochondrial control region sequences from additional locations in the Atlantic and Indian Oceans, confirmed significant matrilineal population structure between the Western and Eastern Atlantic. The combined data also provisionally (i.e., with <em>F</em><sub>ST </sub>but not Φ<sub>ST</sub>) indicated differentiation between Western North and Central-South Atlantic sharks, pointing to the need for further assessment in this region. Matrilineal differentiation was also detected between Indian and Pacific Ocean sharks via pairwise analyses, albeit with the ND4 gene sequence only (Φ<sub>ST</sub> = 0.051; F<sub>ST</sub> = 0.092). Limited sampling in the Pacific leaves open questions about the connectivity dynamics in this large region. Despite the presence of geographic population genetic structure, the mitochondrial data showed no evidence of across ocean basin phylogeographic lineages. A provisional assessment of mitochondrial and nuclear genetic diversity indicated the oceanic whitetip shark's status falls in the middle to upper ranges compared to other shark species, potentially lending some optimism for the present adaptability and resiliency of this species if strong conservation measures are effectively implemented.</p>
Data from: Statistical stream temperature modelling with SSN and INLA: an introduction for conservation practitioners
<p>Statistical stream temperature models can predict the fine-scale spatial distribution of water temperatures and guide species recovery and habitat restoration efforts. However, stream temperature modelling is complicated by spatial autocorrelation arising from non-independence data collected within dendritic networks. We used data from miniature sensors deployed in Canadian Rocky Mountain streams to develop and validate two statistical stream temperature modelling techniques that account for spatial autocorrelation. The first was based on spatial steam network models (SSNs) specifically developed to account for spatial autocorrelation in dendritic stream networks. The second used integrated nested Laplace approximation (INLA) that accounts for spatial autocorrelation but was not designed to address anisotropic stream network data. We evaluated the best-fitted SSN and INLA models using leave-one-out cross validation from the data collected along the stream network. Both modelling techniques had similar RMSE and MAE (near 1<sup>o</sup>C) and r<sup>2</sup> (> 0.6) values, and proved flexible with respect to implementation; however, the SSN models required more preprocessing steps before incorporating spatially correlated random errors. We provide practical advice and open-access data and r-script to help non-experts develop statistical stream temperature models of their own.</p>
Data from: Lineage diversity within a widespread endemic Australian skink to better inform conservation in response to regional-scale disturbance
<p>This dataset was used to examine the phylogeographic genetic structure of Eastern three lined skink<em> Bassiana duperreyi</em>. It comprises SNP data used for population genetics and phylogenetic reconstruction. The data were used to provide foundational work for the detailed taxonomic re-evaluation of this species complex and to reinforce the need for biodiversity assessment to include an examination of cryptic species and/or cryptic diversity below the level of species. Such information on lineage diversity within species and its distribution in the context of disturbance at a regional scale can be factored into conservation planning regardless of whether a decision is made to formally diagnose new species taxonomically and nomenclaturally.</p>
The conservation genetics of Iris lacustris (Dwarf Lake Iris), a Great Lakes endemic
<p><em>Iris lacustris</em>, a northern Great Lakes endemic, is a rare species known from 165 occurrences across Lake Michigan and Huron in the United States and Canada. Due to multiple factors, including habitat loss, lack of seed dispersal, patterns of reproduction, and forest succession, the species is threatened. Early population genetic studies using isozymes and allozymes recovered no to limited genetic variation within the species. To better explore genetic variation across the geographic range of <em>I. lacustris </em>and to identify units for conservation, we used tunable Genotyping-by-Sequencing (tGBS) with 171 individuals across 24 populations from Michigan and Wisconsin, and because the species is polyploid, we filtered the single nucleotide polymorphism (SNP) matrices using polyRAD to recognize diploid and tetraploid loci. Based on multiple population genetic approaches, we resolved three to four population clusters that are geographically structured across the two ranges of the species. The species migrated from west to east across its geographic range, and minimal genetic exchange has occurred among populations. Four units for conservation are recognized, but nine adaptive units were identified, providing evidence for local adaptation across the geographic range of the species. Population genetic analyses with all, diploid, and tetraploid loci recovered similar results, which suggests that methods may be robust to variation in ploidy level. </p>
Targeting fin whale conservation in the North-Western Mediterranean Sea: Insights on movements and behaviour from biologging and habitat modelling
<p>Biologging and habitat modelling are key tools supporting the development of conservation measures and mitigating the effects of anthropogenic pressures on marine species. Here, we analysed satellite telemetry data and foraging habitat preferences in relation to chlorophyll-a productivity fronts to understand the movements and behaviour of endangered Mediterranean fin whales (<em>Balaenoptera physalus)</em> during their spring-summer feeding aggregation in the North-Western Mediterranean Sea. Eleven individuals were equipped with Argos satellite transmitters across three years, with transmissions averaging 23.5 ± 11.3 days. Hidden Markov Models were used to identify foraging behaviour, revealing how individuals showed consistency in their use of seasonal core feeding grounds; this was supported by the distribution of potential foraging habitat. Importantly, tracked whales spent most of their time in areas with no explicit protected status within the study region. This highlights the need for enhanced time- and place-based conservation actions to mitigate the effects of anthropogenic impacts for this species, notably ship strike risk and noise disturbance in an area of exceptionally high maritime traffic levels. These findings strengthen the need to further assess critical habitats and Important Marine Mammal Areas that are crucial for focussed conservation, management, and mitigation efforts.</p>
Data for: Shifts in plant-invertebrate interactions between wild and ex-situ conservation populations of a critically endangered tree
<p>Ex-situ conservation is an effective approach to prevent the extinction of endangered species. Biotic interactions (eg herbivory and pollination) are critical to ex-situ conservation success, including plant establishment, survival, and reproduction. However, shifts in biotic interactions between wild and ex-situ populations are still poorly understood. We compared herbivory and pollination characteristics between the only wild population (WP) and three ex-situ populations (LP, local population, nearby WP; NP, north population, ca. 850 km; and SP, south population, ca. 750 km) of a critically endangered tree species (<em>Sinojackia huangmeiensis</em>) to explore the latitudinal changes in plant-invertebrate interactions. Larvae of the Limacodidae family were the dominant herbivores in WP, LP, and NP, while the only herbivore observed in SP was a snail. Compared to WP, the leaf herbivory rate was unchanged in LP but decreased in NP and SP. Leaf defense traits (total phenols, tannins, leaf thickness, and leaf dry matter content) increased or remained unchanged in the three ex-situ populations. A pollinator (<em>Apis cerana</em>) of <em>S. huangmeiensis</em> was present in the four populations. NP and SP lacked some pollinators that were found in both WP and LP, but they shared one pollinator that was not observed in WP and LP. The pollinator visiting frequency increased in SP, while it did not change significantly in LP and NP. Synthesis and applications: Our results suggested that both herbivory and pollination of <em>S. huangmeiensis</em> changed in ex-situ populations, with complete or partial changes in herbivores, leaf herbivory rate, pollinators, pollinator visiting frequency, and fruit set in the two distant ex-situ populations. This work provides a unique empirical study of shifts in both antagonistic and mutualistic biotic interactions between wild and ex-situ populations. We emphasized that it is essential to integrate herbivore and pollinator management in future ex-situ conservation of plant species.</p>
Figure 4 in Natural regeneration in Atlantic Forest Fragments: using ants (Hymenoptera: Formicidae) for monitoring a conservation unit
Figure 4. Camponotus cillae recorded for the RPPN Botujuru: (A) front view; (B) dorsal view; (C) side view.
Population genetics of caribou in the Alaska-Yukon border region: implications for designation of conservation units and small herd persistence
<p>Better knowledge of genetic relationships between the Fortymile caribou herd and its neighbors is needed for conservation decision-making in Canada. Here, we contribute the first fine-scale analysis of genetic population structure in nine contiguous caribou herds at the geographic boundaries between Barren-ground and Northern Mountain caribou, and at the Alaska-Yukon border. Using pairwise differentiation metrics, STRUCTURE, and discriminant analysis of principal components (DAPC) to analyze 15 microsatellite loci in 379 caribou, we found complex patterns of genetic differentiation. The Fortymile was the only herd assigned to more than one genetic cluster, indicative of its history as a larger herd whose range expansions and gene flow to other herds were likely important to maintaining diversity across a functioning genetic metapopulation. Some small herds (Chisana, Klaza, and White Mountains) were genetically distinct, while others (Hart River, Clear Creek, Mentasta) exhibited little differentiation from herds they occasionally overlap, including herds assigned to different conservation units (DUs). This genetic connectivity does not result from demographic connectivity, as episodic contact during rut, rather than herd switching, is the likely mechanism. Unusually, one small herd (White Mountains) maintained genetic differentiation despite rut overlap with Fortymile. Our data reveal that some herds with different ecological and behavioral attributes are demographically independent but nonetheless genetically connected. Thus, we suggest that managing caribou for an appropriate level of genetic connectivity, while also supporting herd persistence, will be essential to conserve caribou genetic diversity in the region.</p>
Conserved patterns and locomotor-related evolutionary constraints in the hominoid vertebral column
<div> <div> <div> <p>The evolution of the hominoid lineage is characterized by pervasive homoplasy, notably in regions such as the vertebral column, which plays a central role in body support and locomotion. Few isolated and fewer associated vertebrae are known for most fossil hominoid taxa, but identified specimens indicate potentially high levels of convergence in terms of both form and number. Homoplasy thus complicates attempts to identify the anatomy of the last common ancestor of hominins and other taxa and stymies reconstructions of evolutionary scenarios. One way to clarify the role of homoplasy is by investigating constraints via phenotypic integration, which assesses covariation among traits, shapes evolutionary pathways, and itself evolves in response to selection. We assessed phenotypic integration and evolvability across the subaxial (cervical, thoracic, lumbar, sacral) vertebral column of macaques (<em>n</em> = 96), gibbons (<em>n</em> = 77), chimpanzees (<em>n</em> = 92), and modern humans (<em>n</em> = 151). We found a mid-cervical cluster that may have shifted cranially in hominoids, a persistent thoracic cluster that is most marked in chimpanzees, and an expanded lumbosacral cluster in hominoids that is most expanded in gibbons. Our results highlight the highly conserved nature of the vertebral column. Taxa appear to exploit existing patterns of integration and ontogenetic processes to shift, expand, or reduce cluster boundaries. Gibbons appear to be the most highly derived taxon in our sample, possibly in response to their highly specialized locomotion.</p> </div> </div> </div>
Population genomics of flat-tailed horned lizards (Phrynosoma mcallii) informs conservation and management across a fragmented Colorado Desert landscape
<p><em>Phrynosoma mcallii</em> (flat-tailed horned lizards) is a species of conservation concern in the Colorado Desert of the United States and Mexico. We analyzed ddRADseq data from 45 lizards to estimate population structure, infer phylogeny, identify migration barriers, map genetic diversity hotspots, and model demography. We identified the Colorado River as the main geographic feature contributing to population structure, with the populations west of this barrier further subdivided by the Salton Sea. Phylogenetic analysis confirms that northwestern populations are nested within southeastern populations. The best-fit demographic model indicates Pleistocene divergence across the Colorado River, with significant bidirectional gene flow, and a severe Holocene population bottleneck. These patterns suggest that management strategies should focus on maintaining genetic diversity on both sides of the Colorado River and Salton Sea. We recommend additional lands in the U.S. and Mexico that should be considered for similar conservation goals as those in the Rangewide Management Strategy (RMS). We also recommend periodic rangewide genomic sampling to monitor ongoing attrition of diversity, hybridization, and changing structure due to habitat fragmentation, climate change and other long-term impacts.</p>
Conservation opportunities through improved management of recently established protected areas in Southeast Asia
<p> </p> <ul> <li>The 'Table_PA_info' contains information on all 80 Protected Areas analyzed in this study.</li> <li>The 'Synth_plots' includes synthetic control model fits for all 80 Protected Areas analyzed in this study. The number displayed on the top-left of each plot corresponds to the WDPA-ID of the respective protected area. For further details on each protected area, please refer to the 'Table_PA_info' document.</li> <li>The 'placebos' document consists of placebo test plots. The number on the top-left of each plot indicates the WDPA-ID of the protected area. Refer to the 'Table_PA_info' document for more information on each protected area.</li> </ul>
Collecting baleen whale blow samples by drone: a minimally intrusive tool for conservation genetics
<p>In coastal British Columbia, Canada, marine megafauna such as humpback whales (<em>Megaptera novaeangliae</em>) and fin whales (<em>Balaenoptera physalus velifera</em>) have been subject to a history of exploitation and near extirpation. While their populations have been in recovery, significant threats are posed by proposed natural resource ventures in this region, in addition to the compounding effects of increasingly severe marine heatwaves. Genetic tools play a vital role in informing conservation efforts, but the associated collection of tissue biopsy samples can be challenging for the investigators and disruptive to the ongoing behaviour of the targeted whales. Here we evaluate a minimally intrusive approach based on collecting exhaled breath condensate, or respiratory 'blow' samples, from baleen whales using an unoccupied aerial system (UAS), within Gitga'at First Nation territory for conservation genetics. Minimal behavioural responses to the sampling technique were observed, with no response detected 87% of the time (of 112 UAS deployments). DNA from whale blow (<em>n</em> = 88 samples) was extracted, and DNA profiles consisting of 10 nuclear microsatellite loci, sex identification, and mitochondrial (mt) DNA haplotypes, were constructed. An average of 7.5 microsatellite loci per individual were successfully genotyped. The success rates for mtDNA and sex assignment were 80% and 89% respectively. Thus, this minimally intrusive sampling method can be used to describe genetic diversity and generate genetic profiles for individual identification. The results of this research show the potential of UAS-collected whale blow for conservation genetics from a remote location.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.