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FIGURE 11. Humbertium ithorense. RMNH.VER.20261.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 11. Humbertium ithorense. RMNH.VER.20261.a (field number RS83). Representation of four different structures of the copulatory apparatus (fgd, female genital duct; od, ovovitelline duct; sd, sperm duct; sv, seminal vesicle), based on a 3D reconstruction obtained from sagittal sections using the program Free-D (Andrey & Maurin 2005). A. High angle view of the female genital duct and oviducts; B. Dorsal view; C. Lateral view from the left side. Postero-dorsal entrance is indicated by yellow arrowheads. Capital letters at grey arrowheads indicate orientation: P, posterior; A, anterior; D, dorsal; V, ventral; L, left; R, right.

opennotspecifiedAug 2023View details →
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FIGURE 10. Humbertium ithorense. RMNH.VER.20261.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 10. Humbertium ithorense. RMNH.VER.20261.a (field number RS83). Sagittal reconstruction (A) and photomicrograph of sagittal section (B) of the copulatory apparatus; anterior to the right.

opennotspecifiedAug 2023View details →
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FIGURE 15. Humbertium ithorense. RMNH.VER.20261.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 15. Humbertium ithorense. RMNH.VER.20261.a (field number RS83). Photomicrographs. A. Sagittal section of the female genital duct, anterior to the right. Notice the number of erythrophil secretions indicated by white arrowheads; B. Section showing a part of the copulatory apparatus; anterior to the right. Notice the fold of the common atrium filled with erythrophil secretion, indicated by a white arrowhead.

opennotspecifiedAug 2023View details →
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FIGURE 9. Bipalium kewense. RMNH.VER.20259.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 9. Bipalium kewense. RMNH.VER.20259.a (field number KSL2400.2). Photomicrograph of sagittal section of the copulatory apparatus; anterior to the left.

opennotspecifiedAug 2023View details →
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FIGURE 19. Novibipalium rhynchophorum. A and B. Holotype ZMA.V in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 19. Novibipalium rhynchophorum. A and B. Holotype ZMA.V.Pl.3053.a (field number KSL2381.1). Sagittal reconstruction of the copulatory apparatus (A) and photomicrograph of sagittal section of the copulatory apparatus (B); anterior to the left; C. Paratype ZMA.V.Pl.3053.b (field number KSL2381.2). Photomicrograph of sagittal section of the copulatory apparatus; anterior to the right.

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FIGURE 8. Bipalium rugiantrum. RMNH.VER.20258.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 8. Bipalium rugiantrum. RMNH.VER.20258.a (field number KSL2402). Sagittal reconstruction (A) and photomicrograph of sagittal section (B) of the copulatory apparatus; anterior to the left.

opennotspecifiedAug 2023View details →
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FIGURE 7. Bipalium rugiantrum. RMNH.VER.20258.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 7. Bipalium rugiantrum. RMNH.VER.20258.a (field number KSL2402). A. Schematic drawing of a live specimen based on the photograph published by Yamamoto et al. (2003, Fig. 32) and notes on the preserved specimen; scale bar not available; B. Photograph of the dorsal pattern at the prepharyngeal region of the preserved specimen; C. Schematic drawing of the ventral side, based on Yamamoto et al. (2003, Fig. 38) and notes on the preserved specimen

opennotspecifiedAug 2023View details →
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FIGURE 27. Diversibipalium marginatanigrum. RMNH.VER.21027.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 27. Diversibipalium marginatanigrum. RMNH.VER.21027.a (field number RS84). A. Photograph of live specimen; scale bar not available; B. Schematic drawing of the preserved specimen on its left side; dorsal dark band is not visible.

opennotspecifiedAug 2023View details →
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FIGURE 31. Diversibipalium contortolineatum. Photographs. A. Holotype RMNH.VER.21030.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 31. Diversibipalium contortolineatum. Photographs. A. Holotype RMNH.VER.21030.a (field number RS581.2), lateral view of its right side, and the smaller paratype RMNH.VER.21030.c (field number RS581.3) in dorsal view; B. Holotype RMNH.VER.21030.a (field number RS581.2), lateral view of its left side and partial view of the smaller paratype RMNH. VER.21030.c (RS581.3); C. Holotype RMNH.VER.21030.a (field number RS581.2) in dorsal view and the smaller paratype RMNH.VER.21030.c (field number RS581.3) on its ventral side; D. Holotype RMNH.VER.21030.a (field number RS581.2) with dorsal view of the head and anterior end, and ventral view of the median part of the body; E. Paratype RMNH.VER.21030. b (field number RS581.1), anterior part of the body in lateral view of its right side; F. Paratype RMNH.VER.21030.b (field number RS581.1), dorsal view of the posterior half of the body.

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FIGURE 30. Diversibipalium duplalaticlavium. RMNH.VER.21029.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 30. Diversibipalium duplalaticlavium. RMNH.VER.21029.a (field number RS580). A. Schematic drawing of the posterior part of the specimen after it accidentally broke into two parts. Anterior to the left.; B. Photograph of posterior part of the preserved specimen; C. Photograph of entire preserved specimen on its left side. The clear budge posterior to the white patches may correspond to the copulatory apparatus (cap?). Asterisks highlight white dorsal patches. Curly brackets indicate the part where mucus is not covering the body and the true brownish colouration can be distinguished.

opennotspecifiedAug 2023View details →
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FIGURE 6. Bipalium rhytidoantrum. RMNH.VER.20257.1 in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 6. Bipalium rhytidoantrum. RMNH.VER.20257.1 (field number KSL2401). Sagittal reconstruction (A) and photomicrograph of sagittal section (B) of the copulatory apparatus; anterior to the right.

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FIGURE 26. Umbotectum flagellum. A in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 26. Umbotectum flagellum. A. Drawing of the anterior part of the preserved specimen; modified after de Beauchamp (1940). B. Reconstruction of the copulatory apparatus; modified after de Beauchamp (1940). Asterisk (*) indicates the characteristic flagellum present in this species.

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FIGURE 29. Diversibipalium duplalaticlavium. RMNH.VER.21029.a in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 29. Diversibipalium duplalaticlavium. RMNH.VER.21029.a (field number RS580). Photographs. A. Dorsal view of preserved specimen; most of the specimen is covered by a mucus layer but at the posterior part the real brownish colouration can be distinguished; B. Dorsal view of the anterior part of the head; C. Ventral view.

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FIGURE 3 in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 3. Calibrated tree on the basis of the ribosomal gene 18S, including a large number of representatives of the phylum Platyhelminthes. Calibration points (CP) indicated with filled blue circles on the nodes. Yellow bars represent highest posterior density (HPD) 95% confidence intervals for the estimated ages of the nodes. In this tree, Bornean Diversibipalium species D. contortolineatum (RMNH.VER.21030.b, field number RS581.1) and D. duplalaticlavium (RMNH.VER.21029.a, field number RS580) are set as sister to the rest of the Bipaliinae.

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FIGURE 5. Bipalium rhytidoantrum. RMNH.VER.20257.1 in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 5. Bipalium rhytidoantrum. RMNH.VER.20257.1 (field number KSL2401). A and B. Photomicrographs of sagittal sections of the anterior part, showing various anatomical features; anterior to the right.

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FIGURE 1 in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 1. Approximate native distribution (in yellow) of the subfamily Bipaliinae, modified from von Graff (1899) and Ogren et al. (1992). Localities of new species described in the present paper are indicated with numbers: 1) Bipalium rhytidoantrum; 2) B. rugiantrum; 3) Humbertium ithorense; 4) Novibipalium rhynchophorum; 5) Umbotectum capitofalcatum; 6) U. t-maculatum; 7) U. flagellum; 8) Diversibipalium marginatanigrum; 9) D. castaneum; 10) D. cephalolacteum; 11) D. contortolineatum; 12) D. duplalaticlavium. Introduced species (present worldwide, excepting Antarctica) have been excluded from the map.

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FIGURE 4. Bipalium rhytidoantrum. A in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 4. Bipalium rhytidoantrum. A. Schematic drawing of a live specimen based on a photograph published by Yamamoto et al. (2003, Fig. 28) and on notes and drawings of the preserved specimen (B and C); scale bar not available; B and C. Schematic drawing of the dorsal side of the anterior (B) and posterior (C) parts of preserved holotype specimen RMNH.VER.20256.a (field number KSL2401). A small piece between these two parts was used for DNA extraction.

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FIGURE 2. A in Molecular phylogenetics facilitates the first historical biogeographic analysis of the hammerhead worms (Platyhelminthes: Tricladida: Bipaliinae), with the description of twelve new species and two new genera

FIGURE 2. A. Bayesian phylogenetic tree obtained from three concatenated genes (COI, 18S, 28S), including most of the species described in the present work, plus other Bipaliinae representatives available from GenBank. Supports of Bayesian Inference (Posterior Probability) as well as Maximum Likelihood (Bootstrap) indicated at the nodes (PP/BS), with filled black circles denoting full support (1/100). Scale bar: substitutions per site. Geographic names in red in the right-hand column indicate that the species have been introduced into these regions and that the original distributional range is unknown, excepting D. multilineatum, which probably originates from Japan. Note that taxonomic assignment to the genus Bipalium has been restricted to those species from Japan that are anatomically similar to the type species B. fuscatum; B. Summary of the basic topology of the tree.

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FIGURE 4 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 4. Maximum Likelihood (ML) phylogeny inferred with dataset 3 that contains cox1 and ITS1 sequences concatenated. Tree scale corresponds to the number of substitutions per site. Numbers at nodes reflect the UFB support values proportional to size (showing only values from 70% to 100%). Countries of origin labelled as 3-digit alpha code: ESP (Spain), GBR (UK), NZL (New Zealand), PAN (Panama). Results from species in this study are highlighted in red (Australopacifica atrata), blue (Artioposthia exulans), and green (Marionfyfea adventor).

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FIGURE 2. Maximum likelihood phylogeny inferred from cox1 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 2. Maximum likelihood phylogeny inferred from cox1 sequences (dataset 1). Tree scale corresponds to the number of substitutions per site. Numbers at nodes correspond to the ultrafast bootstrap support values (showing only values from 70% to 100%). Countries of origin labelled as 3-digit alpha code: AUS (Australia), BRA (Brazil), ESP (Spain), FRA (France), GBR (UK), NZL (New Zealand), PAN (Panama). Results from species in this study are highlighted in red (Australopacifica atrata), blue (Artioposthia exulans), and green (Marionfyfea adventor).

opennotspecifiedAug 2023View details →

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