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FIGURE 1 in Larvae of Ancyronyx Erichson, 1847 (Insecta: Coleoptera: Elmidae) from Sulawesi, using DNA sequences for the assignment of the larval stages
FIGURE 1. Statistical parsimony tree of Cox1 mtDNA haplotypes. The tree is scaled horizontally by the number of substitutions, "missing haplotypes" not indicated. Circles represent existing haplotypes from the sample set, with circle size indicating the number of specimens with identical haplotypes at the respective position.
FIGURES 2–11. Stenelmis puberula larva, 2 in Stenelmis puberula Reitter (Coleoptera: Elmidae)-description of larva and its association with adults, using DNA sequences
FIGURES 2–11. Stenelmis puberula larva, 2) head, dorsal view; 3) head, dorso-lateral view; 4) head, ventral view; 5) antenna, dorsal view; 6) antenna, detail of pedicel basis; 7) antennal flagellum and sensorium; 8) labrum, dorsal view; 9) labrum, detail of admedian apical tufted scale; 10) labrum, ventral view; 11) same, detail of serrate scales on anterior margin.
FIGURE 12 in The identity of the Javan Krait, Bungarus javanicus Kopstein, 1932 (Squamata: Elapidae): evidence from mitochondrial and nuclear DNA sequence analyses and morphology
FIGURE 12. Adult male Bungarus candidus (UK B36) from the area of Losarang (Kabupaten Indramayu, West Java, Indonesia) with reduced black bands on the posterior half of the body. Photo by Ulrich Kuch.
FIGURE 3 in The identity of the Javan Krait, Bungarus javanicus Kopstein, 1932 (Squamata: Elapidae): evidence from mitochondrial and nuclear DNA sequence analyses and morphology
FIGURE 3. Ventral view of the type specimen of Bungarus javanicus (RMNH 9007). Photo by Ulrich Kuch.
Figure 13 in Rediscovery and description of Paramormyrops sphekodes (Sauvage, 1879) and a new cryptic Paramormyrops (Mormyridae: Osteoglossiformes) from the Ogooué River of Gabon using morphometrics, DNA sequencing and electrophysiology
Figure 13. Variation in P. ntotom sp. nov. illustrated by five specimens from the Ogooué River: CUMV 96811 tag number JPS-1117, female, 117 mm; CUMV 98091 tag number JPS-1175, male 130 mm SL; CUMV 98092 tag number JPS-1176, male, 154 mm SL; CUMV 98134 tag number JPS-1185, male, 176 mm and CUMV 98136 tag number JPS-1187, male 165 mm.
Figure 15 in Rediscovery and description of Paramormyrops sphekodes (Sauvage, 1879) and a new cryptic Paramormyrops (Mormyridae: Osteoglossiformes) from the Ogooué River of Gabon using morphometrics, DNA sequencing and electrophysiology
Figure 15. EODs from (A) P. sphekodes from the Ogooué River, (B) P. curvifrons from the Ivindo River and (C) P. nototom sp. nov. from the Ogooué River. Each is plotted on the same time scale, all with head positivity upwards. (D) Scatter plot of EOD duration versus the ratio W1/W2 – the widths of the first and second phases of the EOD illustrated in Figs 10 and 14. EOD total duration and W1/W2 overlap for the allopatric pair, P. curvifrons and P. ntotom sp. nov., but not for the sympatric pair, P. sphekodes and P. ntotom sp. nov.
Figure 8 in Rediscovery and description of Paramormyrops sphekodes (Sauvage, 1879) and a new cryptic Paramormyrops (Mormyridae: Osteoglossiformes) from the Ogooué River of Gabon using morphometrics, DNA sequencing and electrophysiology
Figure 8. Electric organ discharges (EODs) from P. sphekodes. (A) Paramormyrops sphekodes specimen CUMV 98177 (tag JPS-1238), a male from type locality showing normalized voltage as a function of time, that is 1.0 V peak-to-peak. Head positivity is upwards on all traces. There are two peaks to the waveform: P1, which is head-positive, and P2, which is headnegative. EOD duration is measured between points marked by open circles – the first and last points exceed ±0.02 V. W1 and W2 are the widths of the first and second peaks. The thin line is a 20× vertical expansion of the waveform which rises gradually from the baseline with no indication of a head-negative pre-pulse, P0, that is present in some other species. The final overshoot, due to AC-coupling of the amplifier, is absent when making DC-coupled recordings. The dashed line indicates the zero baseline. (B) Time derivative, dV/dt, of EOD waveform in (A) has a single positive peak (arrow) in advance of peak P1 and there is no inflection point on the rising phase before P1. (C) EODs from nine specimens of P. sphekodes showing the stereotypy of the species waveform. After normalization to unity peak-to-peak height, all EODs are centred on the zero-crossing between P1 and P2 [zc in (A)]. Males are plotted in blue and females are plotted in red. (D) Power spectral density of EODs of P. sphekodes in (A) with its maximum power at 1125 Hz. Flow and Fhi are the frequencies where the spectral power drops 3 dB below the peak power of the FFT. The bandwidth of the power spectrum is Fhi–Flow. (E) Superimposed power spectra for the nine EODs shown in (C) with peak frequencies marked with 'x'. Axes units are as in D.
Figure 14 in Rediscovery and description of Paramormyrops sphekodes (Sauvage, 1879) and a new cryptic Paramormyrops (Mormyridae: Osteoglossiformes) from the Ogooué River of Gabon using morphometrics, DNA sequencing and electrophysiology
Figure 14. Electric organ discharges (EODs) from P. ntotom sp. nov. EOD waveforms plot voltage versus time. Voltage is normalized by setting the peak-to-peak voltage to 1.0 V. The time base is in (C). (A) EOD of holotype, CUMV 98138 tag number JPS-1189, male, SL 178 mm. The first peak, P1, is head-positive and the second, P2, is head-negative. Overall duration is 5.813 ms, measured between the two red dots. The 20× expanded trace (black) indicates the absence of a head-negative phase preceding P1. Abbreviations are as in Figure 10. (B) Time derivative of EOD shown in (A), indicating two points where dV/dt goes through a local maximum leading to inflection points in the EOD (dashed lines). (C) Superimposed EODs from 18 males (blue) and 13 females and juveniles (red) show a clear sex difference in EOD duration. (D) Power spectrum of the EOD of holotype, with magnitude measured in dB relative to the peak power plotted against frequency in Hz. Frequency at peak power indicated by F max. The frequencies where the magnitude of the power spectrum drops 3 dB below the peak power are indicated by Flow and Fhi. (E) Superimposed power spectra of traces shown in (C) demonstrate that spectral power emphasizes higher frequencies among females compared to males, as expected.
FIGURE 2 in Colour patterns to sequences: a perspective on the systematics of the Hyperolius viridiflavus group (Anura: Hyperoliidae) using mitochondrial DNA
FIGURE 2. ML phylogeny based on 16S rRNA sequences. Branch support is given as SH-aLT/Ultrafast bootstrap, both as percentages. Scale bar equals substitutions per site.
FIGURE 13 in Taxonomy of European Damaeidae (Acari, Oribatida) XI. European species of the genus Piribelba Miko 2021: redescriptions of P. rossica (Bulanova-Zachvatkina 1957) and P. piriformis (Mihelčič, 1964) using morphology and DNA sequence data
FIGURE 13. Piribelba rossica (Bulanova-Zachvatkina, 1957), protonymph from Kemerovo region: A—leg I, right, antiaxial view; B—leg II, right, antiaxial view; C—leg III, right, antiaxial view; D—leg IV, right, antiaxial view. Scale bar 100 μm.
FIGURE 6 in Description of nymphs and female subimago of Sparsorythus multilabeculatus Sroka & Soldán, 2008 (Ephemeroptera: Tricorythidae) associated with male imago based on DNA sequence data
FIGURE 6. Sparsorythus multilabeculatus, nymph, 6a. labrum; 6b. maxilla; 6c. left mandible; 6d. right mandible; 6e. left prostheca; 6f. right prostheca; 6g. hypopharyngeal lingua; 6h. labium. Scale bars: 0.1 mm (6a–6d, 6g, 6h); 0.01 mm (6e, 6f).
FIGURE 3. A in Description of nymphs and female subimago of Sparsorythus multilabeculatus Sroka & Soldán, 2008 (Ephemeroptera: Tricorythidae) associated with male imago based on DNA sequence data
FIGURE 3. A Maximum Likelihood phylogenetic reconstruction based on sequences of the mitochondrial COI gene. Sparsorythus buntawensis, Sparsorythus gracilis, Sparsorythus sescarorum and Tricorythodes explicates sequences from GenBank from aligned COI sequence of 452 bp. Nodal support values are bootstrap values (percentage of 1000 replicate). Scale bar indicates 0.05 nucleotide substitutions.
FIGURE 8 in Description of nymphs and female subimago of Sparsorythus multilabeculatus Sroka & Soldán, 2008 (Ephemeroptera: Tricorythidae) associated with male imago based on DNA sequence data
FIGURE 8. Sparsorythus multilabeculatus, section of segment VII abdominal terga. Scale bars: 0.1 mm.
Fig. 4 in Phylogenetic analysis of the genus Argia Rambur, 1842 (Odonata: Coenagrionidae), based on morphological characters of larvae and mitochondrial DNA sequences
Fig. 4 Evolutionary scenario for the larval morphology inferred by direct optimization of characters on one of the two most parsimonious trees recovered in the phylogenetic analysis after applying implied weights. Black circles indicate transformation of unique characters; white circles indicate parallelism or reversion. The number above the circle indicates
Fig. 2 in Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences
Fig. 2 Phylogenetic tree for Ranunculus species based on plastid regions (rpl32-trnL, rps16-trnQ, trnK-matK, ycf6-psbM). a Consensus tree inferred from the two most parsimonious trees (CI=0.76; RI=0.93). Numbers above branches show bootstrap values (3,000 replicates). b
Fig. 4 in Mitochondrial DNA sequences suggest unexpected phylogenetic position of Corso-Sardinian grass snakes (Natrix cetti) and do not support their species status, with notes on phylogeography and subspecies delineation of grass snakes
Fig. 4 Geographic distribution of mitochondrial clades in grass snakes. Symbols correspond to Fig. 1
Fig. 1 in Mitochondrial DNA sequences suggest unexpected phylogenetic position of Corso-Sardinian grass snakes (Natrix cetti) and do not support their species status, with notes on phylogeography and subspecies delineation of grass snakes
Fig. 1 Maximum likelihood (ML) tree for Natrix sequences calculated with RAxML based on 3,806 bp of mtDNA (ND1, ND2, ND4, cyt b). Numbers above nodes are thorough bootstrap values (RAxML); below nodes, Bayesian posterior probabilities and bootstrap values obtained under maximum parsimony (MP; not shown for some terminal clades with short branch lengths). For new samples, voucher codes (Table 1)
Fig. 3 in Mitochondrial DNA sequences suggest unexpected phylogenetic position of Corso-Sardinian grass snakes (Natrix cetti) and do not support their species status, with notes on phylogeography and subspecies delineation of grass snakes
Fig. 3 Estimated split ages of grass snake clades and their 95% HPD intervals (grey bars). Narrow grey bars are derived from the dating approach using the post-Messinian reopening of the Strait of Gibraltar as age constraint (calibration point I); wide grey bars, using the Sardinian fossil node constraint (calibration point II). Numbers along nodes refer to Table 2; see there for exact values. The depicted nodal ages are based on calibration point I
Fig. 2 in Mitochondrial DNA sequences suggest unexpected phylogenetic position of Corso-Sardinian grass snakes (Natrix cetti) and do not support their species status, with notes on phylogeography and subspecies delineation of grass snakes
Fig. 2 Parsimony networks for haplotypes of Natrix natrix helvetica, N. n. lanzai, a N. n. helvetica x natrix hybrid (left) and Corso-Sardinian grass snakes (right) based on 3,806 bp of mtDNA (ND1, ND2, ND4, cyt b). The large symbol for N. n. helvetica indicates that this haplotype was found twice; small black circles, missing node haplotypes. Connections between haplotypes show number of mutation steps. Connection of haplotypes in left network enforced; 95% connection limit: 27 steps
Supplementary Table S2. DNA polymorphisms in gene sequences of 56 sea buckthorn accessions based on the analysis of whole-genome sequencing data.
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