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146
datasets available to search
ShareScore release 0.9.0
Dataset results
146 results for “Ca2+”
Fight or flight cardiac responses require augmentation of Ca2+ influx via Rad phosphorylation [RNAseq]
GEO Series GSE198903. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
cAMP Ca2+ NFAT signalling in RPTEC control vs. CAA
GEO Series GSE4956. Homo sapiens. 8 samples. Type: Expression profiling by array.
Dataset related to the article " Nicotinic Acid Adenine Dinucleotide Phosphate Induces Intracellular Ca2+ Signalling and Stimulates Proliferation in Human Cardiac Mesenchymal Stromal Cells"
<p>This record contains raw data related to the article “Nicotinic Acid Adenine Dinucleotide Phosphate Induces Intracellular Ca<sup>2+</sup> Signalling and Stimulates Proliferation in Human Cardiac Mesenchymal Stromal Cells"</p> <p>Abstract</p> <p>Nicotinic acid adenine dinucleotide phosphate (NAADP) is a newly discovered second messenger that gates two pore channels 1 (TPC1) and 2 (TPC2) to elicit endo-lysosomal (EL) Ca<sup>2+</sup> release. NAADP-induced lysosomal Ca<sup>2+</sup> release may be amplified by the endoplasmic reticulum (ER) through the Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release (CICR) mechanism. NAADP-induced intracellular Ca<sup>2+</sup> signals were shown to modulate a growing number of functions in the cardiovascular system, but their occurrence and role in cardiac mesenchymal stromal cells (C-MSCs) is still unknown. Herein, we found that exogenous delivery of NAADP-AM induced a robust Ca<sup>2+</sup> signal that was abolished by disrupting the lysosomal Ca<sup>2+</sup> store with Gly-Phe β-naphthylamide, nigericin, and bafilomycin A1, and blocking TPC1 and TPC2, that are both expressed at protein level in C-MSCs. Furthermore, NAADP-induced EL Ca<sup>2+</sup> release resulted in the Ca<sup>2+</sup>-dependent recruitment of ER-embedded InsP<sub>3</sub>Rs and SOCE activation. Transmission electron microscopy revealed clearly visible membrane contact sites between lysosome and ER membranes, which are predicted to provide the sub-cellular framework for lysosomal Ca<sup>2+</sup> to recruit ER-embedded InsP<sub>3</sub>Rs through CICR. NAADP-induced EL Ca<sup>2+</sup> mobilization via EL TPC was found to trigger the intracellular Ca<sup>2+</sup> signals whereby Fetal Bovine Serum (FBS) induces C-MSC proliferation. Furthermore, NAADP-evoked Ca<sup>2+</sup> release was required to mediate FBS-induced extracellular signal-regulated kinase (ERK), but not Akt, phosphorylation in C-MSCs. These finding support the notion that NAADP-induced TPC activation could be targeted to boost proliferation in C-MSCs and pave the way for future studies assessing whether aberrant NAADP signaling in C-MSCs could be involved in cardiac disorders.</p>
Dataset related to the article "Ca2+ dysregulation in cardiac stromal cells sustains fibro-adipose remodeling in Arrhythmogenic Cardiomyopathy and can be modulated by flecainide"
<p>This record contains raw data related to the article "Ca2+ dysregulation in cardiac stromal cells sustains fibro-adipose remodeling in Arrhythmogenic Cardiomyopathy and can be modulated by flecainide" </p> <p>Abstract</p> <p><strong>Background: </strong>Cardiac mesenchymal stromal cells (C-MSC) were recently shown to differentiate into adipocytes and myofibroblasts to promote the aberrant remodeling of cardiac tissue that characterizes arrhythmogenic cardiomyopathy (ACM). A calcium (Ca<sup>2+</sup>) signaling dysfunction, mainly demonstrated in mouse models, is recognized as a mechanism impacting arrhythmic risk in ACM cardiomyocytes. Whether similar mechanisms influence ACM C-MSC fate is still unknown. Thus, we aim to ascertain whether intracellular Ca<sup>2+</sup> oscillations and the Ca<sup>2+</sup> toolkit are altered in human C-MSC obtained from ACM patients, and to assess their link with C-MSC-specific ACM phenotypes.</p> <p><strong>Methods and results: </strong>ACM C-MSC show enhanced spontaneous Ca<sup>2+</sup> oscillations and concomitant increased Ca<sup>2+</sup>/Calmodulin dependent kinase II (CaMKII) activation compared to control cells. This is manly linked to a constitutive activation of Store-Operated Ca<sup>2+</sup> Entry (SOCE), which leads to enhanced Ca<sup>2+</sup> release from the endoplasmic reticulum through inositol-1,4,5-trisphosphate receptors. By targeting the Ca<sup>2+</sup> handling machinery or CaMKII activity, we demonstrated a causative link between Ca<sup>2+</sup> oscillations and fibro-adipogenic differentiation of ACM C-MSC. Genetic silencing of the desmosomal gene PKP2 mimics the remodelling of the Ca<sup>2+</sup> signalling machinery occurring in ACM C-MSC. The anti-arrhythmic drug flecainide inhibits intracellular Ca<sup>2+</sup> oscillations and fibro-adipogenic differentiation by selectively targeting SOCE.</p> <p><strong>Conclusions: </strong>Altogether, our results extend the knowledge of Ca<sup>2+</sup> dysregulation in ACM to the stromal compartment, as an etiologic mechanism of C-MSC-related ACM phenotypes. A new mode of action of flecainide on a novel mechanistic target is unveiled against the fibro-adipose accumulation in ACM.</p>
Ca2+ influx through ORAI1 and ORAI2 channels regulates chromatin accessibility in CD4+ T cells
GEO Series GSE253187. Mus musculus. 13 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
DREAM regulates gene expression and Ca2+ mobilization in sperm cells
GEO Series GSE27829. Mus musculus. 6 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.