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903 results for “Domestication”
Leaf trait covariation and controls on leaf mass per area (LMA) following cotton domestication
<p class="MsoNormal"><span>The process of domestication has driven dramatic shifts in plant functional traits including leaf mass per area (LMA). It remains unclear whether domestication has produced concerted shifts in the lower-level anatomical traits that underpin LMA and how these traits in turn affect photosynthesis. </span><span>In this study, we investigated controls of LMA and leaf gas exchange by leaf anatomical properties at the cellular, tissue and whole leaf levels, comparing 26 wild and 31 domesticated genotypes of cotton </span><span>(<em>Gossypium</em>). </span><span>As expected, domesticated plants expressed lower LMA, higher photosynthesis and stomatal conductance</span><span>, suggesting a shift towards the 'faster' end of the leaf economics spectrum. At whole-leaf level, variation in LMA was predominantly determined by leaf density (LD) both in wild and domesticated genotypes. At tissue level, higher leaf volume per area (<em>V</em><sub>leaf</sub>) in domesticated genotypes was driven by a simultaneous increase in the volume of epidermal, mesophyll and vascular bundle tissue and airspace, while lower LD resulted from a dilution effect of lower increased volume of palisade tissue and vascular bundle of high mass density by higher increased volume of epidermis and airspace of low mass density. The volume of spongy mesophyll exerted direct control on photosynthesis in domesticated genotypes but only indirect control in wild genotypes. At cellular level, a shift to larger but less numerous cells with thinner cell walls underpinned a lower proportion of cell wall mass, and thus a reduction in LD. </span><span>Taken together, cotton domestication has triggered synergistic shifts in the underlying determinants of LMA but also photosynthesis, at cell, tissue and whole-leaf level, resulting in a marked shift in plant ecological strategy.</span></p>
Comparing conservation and animal welfare professionals' perspectives on domestic cat management (data and scripts)
<p>This repository contains the final datasets and R scripts to run the analyses and create the plots in the main text of this publication.</p> <p> </p>
Evidence of an additional center of apple domestication in Iran, with contributions from the Caucasian crab apple <i>Malus orientalis</I> Uglitzk. to the cultivated apple gene pool
<p>Abstract</p> <p>Divergence processes in crop-wild fruit tree complexes in pivotal regions for plant domestication such as the Caucasus and Iran remain little studied. We investigated anthropogenic and natural divergence processes in apples in these regionsusing 26 microsatellite markers amplified in 550 wild and cultivated samples. We found two genetically distinct cultivated populations in Iran that are differentiated from <em>Malus domestica</em>, the standard cultivated apple worldwide. Coalescent-based inferences showed that these two cultivated populations originated from specific domestication events of <em>Malus orientalis</em> in Iran. We found evidence of substantial wild-crop and crop-crop gene flow in the Caucasus and Iran, as has been described in apple in Europe. In addition, we identified seven genetically differentiated populations of wild apple (<em>M. orientalis</em>), not introgressed by the cultivated apple. Niche modeling combined with genetic diversity estimates indicated that these wild populations likely resulted from range changes during past glaciations. This study identifies Iran as a key region in the domestication of apple and <em>M. orientalis</em> as an additional contributor to the cultivated apple gene pool. Domestication of the apple tree therefore involved multiple origins of domestication in different geographic locations and substantial crop-wild hybridization, as found in other fruit trees. This study also highlights the impact of climate change on the natural divergence of a wild fruit tree and provides a starting point for apple conservation and breeding programs in the Caucasus and Iran.</p> <p>Methods</p> <p>Microsatellite genotyping data for <em>M. orientalis</em>, <em>M. sieversii</em> from Kazakhstan, <em>M. domestica</em> and <em>M. baccata</em> were from previously published studies, including: 207 <em>M. orientalis</em> individuals from Turkey, Armenia and Russia (23 sites, Tables S1 and S2, (Cornille et al., 2013; Cornille et al., 2012)), four apple cultivars from Armenia, 40 “pure” European cultivated <em>M. domestica</em> individuals (<em>i.e.</em>, not introgressed by <em>M. sylvestris</em>) (Tables S1 and S2, (Cornille et al., 2013, 2012)), 20 <em>M. sieversii</em> individuals from Kazakhstan (Cornille et al., 2013) and 22 <em>M. baccata</em> individuals from Russia (Cornille et al., 2012). We collected 257 new samples in 2017 in Iran and in 2018 in Kyrgyzstan (<em>M. sieversii</em>) for this study: 167 <em>M. orientalis</em> individuals from the Hyrcanian Forests and the Zagros region in Iran (Table S1), 48 local Iranian apple cultivars from the <em>Seed and Plant Improvement Institute</em> (Karaj, Iran) (Table S1) and 42 <em>M. sieversii</em> individuals from Kyrgyzstan. Note that for 18 of the 48 local Iranian samples, fruit size was measured (Table S1). Collections meet the requirements of the recently enacted Nagoya protocol on access to genetic resources and the fair and equitable sharing of benefits. Thus, a total of 550 individuals were analyzed, comprising 374 <em>M. orientalis</em>, 48 Iranian and four Armenian apple cultivars, 40 European apple cultivars belonging to <em>M. domestica, </em>62<em> M. sieversii </em>(from Kyrgyzstan and Kazakhstan) and 22<em> M. baccata</em> individuals (details are provided in Table S1).</p> <p>DNA from the new samples (<em>N </em>= 257) was extracted from dried leaves with the NucleoSpin plant II DNA extraction kit (Macherey & Nagel, Düren, Germanyâ) following the manufacturer’s instructions. Multiplex microsatellite PCR amplifications were performed with a multiplex PCR kit (Qiagen Inc.â) for 26 microsatellite markers as previously described (Cornille et al., 2012; Patocchi, Frei, Frey, & Kellerhals, 2009). Note that on each DNA plate, we included three controls, <em>i.e.,</em> one sample of <em>M. orientalis, </em>one of <em>M. sieversii</em> and one of <em>M. domestica</em> for which data were already available (Cornille et al., 2013). Genotypes of the controls were compared with the 2013 dataset. We retained only multilocus genotypes for which < 20 % of the data was missing. The suitability of these markers for population genetics analyses has been demonstrated in previous studies (Cornille et al., 2013; Cornille, Gladieux, & Giraud, 2013; Cornille et al., 2012). </p> <p>Software used</p> <p>-STRUCTURE <a href="https://web.stanford.edu/group/pritchardlab/structure.html">https://web.stanford.edu/group/pritchardlab/structure.html</a></p> <p>-ABCtoolbox: <a href="http://cmpg.unibe.ch/software/ABCtoolbox/">http://cmpg.unibe.ch/software/ABCtoolbox/</a></p> <p>-fastsimcoal2 <a href="http://cmpg.unibe.ch/software/fastsimcoal27/">http://cmpg.unibe.ch/software/fastsimcoal27/</a></p> <p>-Genepop: <a href="https://genepop.curtin.edu.au/">https://genepop.curtin.edu.au</a></p> <p>-R software</p>
Low levels of hybridization between domestic and wild Mallards wintering in the Lower Mississippi Flyway
<p>Mallard ducks are a ubiquitous and socio-economically important game bird in North America. Despite their generally abundant midcontinent population, Mallards in eastern North America are declining, which may be partially explained by extensive hybridization with human-released domestically-derived game-farm Mallards. We investigated the genetic composition of Mallards in the middle and lower Mississippi flyway, key wintering regions for the species. We found that nearly 30% of wild Mallards carried mitochondrial haplotypes derived from domestic mallards present in North America, indicating that the individuals had female game-farm Mallard lineage in their past; however nuclear results identified only 4% of the same sample set as putative hybrids. Recovering 30% of samples with OW A mtDNA haplotypes is concordant with general trends across the Mississippi flyway and this percentage was stable across Mallards we sampled a decade apart. The capture and perpetuation of OW A mtDNA haplotypes is likely due to female breeding structure, whereas reversal of the nuclear signal back to wild ancestry is due to sequential backcrossing and lower and/or declining admixture with game-farm Mallards. Future studies of wild ancestry of Mississippi flyway Mallards will benefit from coupling molecular and spatial technology across flyways, seasons, and years to search for potential transitions of Mallard populations with different genetic ancestry, and whether the genetic ancestry is somehow linked to an individual's natal and subsequent breeding location.</p>
Data from: Pre-domestication bottlenecks of the cultivated seaweed Gracilaria chilensis
<p><em><span>Gracilaria</span></em><span><em> chilensis</em> is the main cultivated seaweed in Chile.</span> <span>The low genetic diversity observed in the Chilean population has been associated with the over-exploitation of natural beds and/or the founder effect that occurred during the post-glacial colonization from New Zealand. How these processes have affected its evolutionary trajectory before farming and incipient domestication is poorly understood. </span><span>In this study, we used 2,232 SNPs to assess how the species' evolutionary history in New Zealand (its region of origin), the founder effect linked to transoceanic dispersion and colonization of South America, and the recent over-exploitation of natural populations have influenced the genetic architecture of <em>G</em>. <em>chilensis</em> in Chile. The contrasting patterns of genetic diversity and structure observed between the two main islands in New Zealand attest to the important effects of Quaternary glacial cycles on <em>G</em>. <em>chilensis</em>. ABC analyses indicated that Chatham Island and South America were colonized independently near the end of the Last Glacial Maximum and emphasized the importance of coastal and oceanic currents during that period. Furthermore, ABC analyses inferred the existence of a recent and strong genetic bottleneck in Chile, matching the period of over-exploitation of the natural beds during the 1970s, followed by rapid demographic expansion linked to active clonal propagation used in farming. Recurrent genetic bottlenecks strongly eroded the genetic diversity of <em>G</em>. <em>chilensis</em> prior to its cultivation, raising important challenges for the management of genetic resources in this incipiently domesticated species. </span></p>
Naturally occurring metals in unregulated domestic wells in Nevada, USA
<p>The dominant source of drinking water in rural Nevada, United States, is privately-owned domestic wells. Because the water from these wells is unregulated with respect to government guidelines, it is the owner's responsibility to test their groundwater for heavy metals and other contaminants. Arsenic, lead, cadmium, and uranium have been previously measured at concentrations above Environmental Protection Agency (EPA) guidelines in Nevada groundwater. This is a public health concern because elevated levels of these metals are known to have negative health effects.</p> <p>We recruited individuals through a population health study, the Healthy Nevada Project, to submit drinking water samples from domestic wells for testing. Water samples were returned from 174 households with private wells. We found 22% had arsenic concentrations exceeding the EPA maximum contaminant level (MCL) of 10 mg/L. Additionally, federal, state, or health-based guidelines were exceeded for 8% of the households for uranium and iron, 6% for lithium and manganese, 4% for molybdenum, and 1% for lead. The maximum observed concentrations of arsenic, uranium, and lead were ~80, ~5, and ~1.5 times the EPA guideline values, respectively. 41% of households had a treatment system and submitted both pre- and post-treatment water samples from their well. The household treatments were shown to reduce metal concentrations, but concentrations above guideline values were still observed. Many treatment systems cannot reduce the concentration below guideline values because of water chemistry, treatment failure, or improper treatment techniques. These results show the pressing need for continued education and outreach on regular testing of domestic well waters, proper treatment types, and health effects of metal contamination. These findings are potentially applicable to other arid areas where groundwater contamination of naturally occurring heavy metals occurs.</p>
Data for: Domestic cat accelerometer data calibrated with behaviours
<p>Observing animals in the wild often poses extreme challenges, but animal-borne accelerometers are increasingly revealing unobservable behaviours. Automated machine learning streamlines behaviour identification from the substantial datasets generated during multi-animal, long-term studies, however, the accuracy of such models depends on the qualities of the training data. We examined how data processing influenced the predictive accuracy of random forest (RF) models, leveraging the easily observed domestic cat (<em>Felis catus</em>) as a model organism for terrestrial mammalian behaviours.</p> <p>Nine indoor domestic cats were equipped with collar-mounted tri-axial accelerometers, and behaviours were recorded alongside video footage. From this calibrated data, eight datasets were derived with; (i) additional descriptive variables; (ii) altered frequencies of acceleration data (40 Hz vs. a mean over 1 second); and (iii) standardised durations of different behaviours. These training datasets were used to generate RF models which were validated against calibrated cat behaviours before identifying behaviours of five free-ranging tag-equipped cats. These predictions were compared to those identified manually to validate the accuracy of the RF models for free-ranging animal behaviours.</p> <p>RF models accurately predicted the behaviours of indoor domestic cats (F-measure up to 0.96) with discernible improvements observed with post-data-collection processing. Additional variables, standardized durations of behaviours, and higher recording frequencies improved model accuracy. However, prediction accuracy varied with different behaviours, where high-frequency models excelled in identifying fast paced behaviours (e.g. locomotion), while lower frequency models (1 Hz) more accurately identified slower, aperiodic behaviours such as grooming and feeding, particularly when examining free-ranging cat behaviours.</p> <p>While RF modelling offered a robust means of behaviour identification from accelerometer data, field validations were important to validate model accuracy for free-ranging individuals. Future studies may benefit from employing similar data processing methods that enhance RF behaviour identification accuracy, with extensive advantages for investigations into ecology, welfare, and management of wild animals.</p>
Figure 1 in Genetic structure of Trypanosoma congolense "forest type" circulating in domestic animals and tsetse flies in the South-West region of Cameroon
Figure 1. Allelic frequency at each locus by host.
Interacting effects of surface water and temperature on wild and domestic large herbivore aggregations and contact rates
<p>Earth's climate is rapidly changing, bringing forth questions of how domestic and wild animals will alter their behavior in response to increasing temperatures and dryland expansion. Dwindling water availability will likely impact animal behavior and water foraging, potentially increasing animal aggregations and interspecific contacts. These interspecific contacts are especially important for competition, predation, and disease transmission among wildlife and domestic animals.</p> <p>In this study, we analyzed interspecific wildlife and cattle contacts using two years of camera trap data at an experimental water manipulation site at a conservancy in central Kenya.</p> <p>We found that on average, the hourly probability of any interspecific contact was approximately 3.4 times higher at water sources versus drained water sources, and 18 times higher than surrounding matrix areas, and that this relationship was amplified by dry and hot conditions.</p> <p>Species-specific analyses revealed variation in the magnitude of responses across wildlife and domestic cattle, although all animals had approximately 2-3 times higher interspecific contact probability with other species at water in hot conditions versus other conditions. Notably, we observed the largest behavioral changes for relatively water-independent species, such as giraffe, which had 3.6 times higher interspecific contact probability at water sources in hot versus other conditions.</p> <p><em>Synthesis and applications:</em> These findings show how elevated temperatures that will become increasingly common with future climate changes can increase interspecific contacts around critical water resources. In mixed wildlife-livestock systems, maintaining wildlife-only water sources may be a practical management tool to mitigate human-wildlife conflict and disease transmission at this interface, especially during dry and hot conditions.</p>
Uncovering the little known impact of a millennia-old traditional use of temperate oak forests: free-ranging domestic pigs markedly change the herb layer, but barely affect the shrub layer
<p>This excel file contains data on ground, herb and shrub layer characteristics of hardwood floodplain forests under different disturbance intensity by free-ranging domestic pigs, a millennia-old practice in Eurasia. We used these dataset to create figures 3, 4, 5, 6 (a) (b), 7, 8 presented in the paper „Uncovering the little known impact of a millennia-old traditional use of temperate oak forests: free-ranging pigs markedly change the herb layer, but barely affect the shrub layer”. Vegetation surveys were conducted in the spring and late summer periods of 2017 and 2018. The bare soil surface and litter cover were visually estimated within each 0.25 ha sampling plot. The cover and composition of the herb layer were recorded using 30 subsamples (1 m2) at each SP (altogether 1680 subsamples). 28 subsamples were located along the four cardinal directions at 2 m distances running from the centre of sampling plots and 2 other subsamples were randomly positioned. The total cover of the upper (higher than 130 cm) and lower (50-130 cm) shrub layer, and the cover of each species by layers, were visually estimated in each 0.25 ha SP. The browsing, rooting and rubbing impacts of pigs were measured along a 56-m-long transect running through the centre of the SPs from north to south. We sampled altogether from 3138 to 3282 woody specimens depending on variables used to describe pigs impacts on specimens.</p>
Figure 1 in Fish domestication in aquaculture: reassessment and emerging questions
Figure 1. – Evolution of global marine capture fisheries since 1950 (based on the FAO database).
The dataset of the manuscript "GPU-HADVPPM4HIP V1.0: higher model accuracy on China's domestically GPU-like accelerator using heterogeneous compute interface for portability (HIP) technology to accelerate the piecewise parabolic method (PPM) in an air quality model (CAMx V6.10)"
<p><strong>bcfile.zip:</strong> the clean boundary condition files.</p> <p><strong>CAMxv6x_cpp.zip: </strong>the source code of CAMx-HIP version which coupled with HIP-HADVPPM scheme.</p> <p><strong>data.zip:</strong> final data tables used to plot figures.</p> <p><strong>emisfile.zip: </strong>the emission files.</p> <p><strong>icfile.zip:</strong> the clean initial condition files.</p> <p><strong>tuvfile.zip </strong>and <strong>o3mapfile.zip:</strong> the photolysis files.</p> <p><strong>outputfile.zip:</strong> the computation results outputted by CAMx model for Fortran version on the Intel Xeon E5-2682 v4 CPU, CUDA version on the NVIDIA K40m and V100 clusters, and HIP version on the China' s domestically heterogeneous cluster A.</p> <p><strong>wrfcamx.zip:</strong> the meteorological files.</p> <p><strong>offline_test_cuda.zip: </strong>the advection module code written in CUDA C language</p> <p><strong>offline_test_fortran.zip:</strong> the advection module code written in Fortran language</p> <p><strong>offline_test_hip.zip: </strong>the advection module code written in HIP C language</p>
Fig. 2 in Generalists at the interface: Nematode transmission between wild and domestic ungulates
Fig. 2. Histogram of liability index, L, for domestic and wild species with degree greater than 10.
Fig. 1 in Wild horse populations in south-east Australia have a high prevalence of Strongylus vulgaris and may act as a reservoir of infection for domestic horses
Fig. 1. Map illustrating location of sampling sites.
VoiceHome-2 corpus : A corpus dedicated to distant-microphone speech processing in domestic environments
<p><strong>Purpose: </strong></p> <p>This corpus includes reverberated, noisy speech signals spoken by 12 native French talkers in 4 houses (3 rooms per house) and recorded by an 8-microphone device at various angles and distances and in various noise conditions.</p> <p>This corpus stands apart from other corpora in the field by the number of rooms and homes considered by the diversity of acoustic conditions recorded and by the facts that it is publicly available at no cost.</p> <p><strong>Other materials:</strong></p> <ul> <li>Article : N. Bertin, E. Camberlein, R. Lebarbenchon, E. Vincent, S. Sivasankaran, I. Illina and F. Bimbot: <a href="https://hal.inria.fr/hal-01923108"><strong>VoiceHome-2, an extended corpus for multichannel speech processing in real homes</strong></a>, <em>Speech Communication</em>, Elsevier : North-Holland, 2019, 106, pp.68-78. <a href="https://dx.doi.org/10.1016/j.specom.2018.11.002">⟨10.1016/j.specom.2018.11.002⟩</a>.</li> <li>Code baseline to reproduce article's results: <ul> <li><a href="https://hal.inria.fr/hal-02963528">Localization and speech enhancement</a></li> <li><a href="https://doi.org/10.5281/zenodo.4079314">Acoustic models</a> and <a href="https://hal.inria.fr/hal-02963802">recognition scripts</a> for automatic speech recognition</li> </ul> </li> <li>Related software to execute the baseline: <ul> <li><a href="https://gitlab.inria.fr/bass-db/mbss_locate">MBSS Locate (v2.0)</a></li> <li><a href="https://gitlab.inria.fr/bass-db/fasst">FASST</a></li> </ul> </li> </ul> <p><strong>Documentation:</strong></p> <p>The corpus documentation is both available into the archive and hereafter by clicking on voiceHome-2_corpus_v1.0_documentation.pdf .</p> <p><strong>Terms of use</strong></p> <p>You may exploit the corpus for a non-commercial scientific purpose provided you mention it in any written work or software you derive from its use. Within a published article, paper or report, the corpus must appear in the bibliographical references.</p> <p><strong>Speaker records diffusion consent</strong></p> <p>All participants have given an informed and signed consent about public diffusion of recorded sentences.</p> <p><strong>Contact:</strong></p> <p>nancy [dot] bertin [at] irisa [dot] fr</p>
voiceHome corpus: A corpus dedicated to distant-microphone speech processing in domestic environments
<p><strong>Purpose: </strong></p> <p>This corpus includes reverberated, noisy speech signals spoken by native French talkers in a lounge and recorded by an 8-microphone device at various angles and distances and in various noise conditions.</p> <p>Room impulse responses and noise-only signals recorded in various real rooms and homes and baseline speaker localization and enhancement software are also provided.</p> <p>This corpus stands apart from other corpora in the field by the number of rooms and homes considered and by the fact that it is publicly available at no cost.</p> <p> </p> <p><strong>Other materials:</strong></p> <ul> <li>Article: N. Bertin, E. Camberlein, E. Vincent, R. Lebarbenchon, S. Peillon, E. Lamandé, S. Sivasankaran, F. Bimbot, I. Illina, A. Tom, S. Fleury and E. Jamet: <a href="https://hal.inria.fr/hal-01343060"><strong>A French corpus for distant-microphone speech processing in real homes</strong></a>, Interspeech2016, Sep 2016, San Francisco, United States, 2016.</li> <li>Related software to reproduce article's results: <ul> <li><a href="https://gitlab.inria.fr/bass-db/mbss_locate">Multi-Channel BSS Locate (v1.3)</a></li> <li><a href="https://gitlab.inria.fr/bass-db/fasst">FASST (v2.2.1)</a></li> </ul> </li> </ul> <p><strong>Documentation (in french):</strong></p> <p>The corpus documentation is both available into the archive and hereafter by clicking on voiceHome_corpus_french_documentation_v1.2.pdf .</p> <p><strong>Terms of use</strong></p> <p>You may exploit the corpus for a non-commercial scientific purpose provided you mention it in any written work or software you derive from its use. Within a published article, paper or report, the corpus must appear in the bibliographical references.</p> <p><strong>Speaker records diffusion consent</strong></p> <p>All participants have given an informed and signed consent about public diffusion of recorded sentences.</p> <p><strong>New corpus version available : voiceHome-2 corpus</strong></p> <p>A new version of the corpus is available : <a href="https://doi.org/10.5281/zenodo.1252143"><strong>voiceHome-2 corpus web page</strong></a></p> <p> </p>
Raw data belonging to paper "Calculating Retail Prices from Demand Response Target Schedules to Operate Domestic Electric Water Heaters"
<p>The Zip file contains the raw data used for drawing conclusions in the paper "Calculating Retail Prices from Demand Response Target Schedules to Operate Domestic Electric Water Heaters" accepted for publication in Energy Informatics 2018.</p> <p>The raw data is the parameters of a sample of 50 domestic electric water heaters (DEWHs)<br> used for evaluating the algorithm in the paper. It is explained in the readme.txt.</p>
Data for: Soil domestication by rice cultivation results in plant-soil feedback through shifts in soil microbiota
<p>This repository contains the data for the manuscript entitled "Soil domestication by rice cultivation results in plant-soil feedback through shifts in soil microbiota." Analysis scripts can be found at <a href="https://github.com/bulksoil/SoilDomestication">https://github.com/bulksoil/SoilDomestication</a></p>
Figure 1 in Competition for food between the markhor and domestic goat in Chitral, Pakistan
Figure 1. Location of study area.
Fig. 1 in Taenia lynciscapreoli in semi-domesticated reindeer (Rangifer tarandus tarandus, L.) in Sweden
Fig. 1. Taenia lynciscapreoli. Photo Anton de Jong, SVA.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.