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opencc-by-4.0Oct 2024View details →

Intravital imaging data of resident tissue macrophages in the peritoneum

Imaging data for the manuscript titled 'Cellular morphodynamics as quantifiers for functional states of resident tissue macrophages in vivo', to be submitted to PLOS Computational Biology

Fig. 4. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways

Fig. 4. S. frutescens inhibits LPS-induced HMOX-1 upregulation in RAW 264.7 cells. Data represent mean ± SEM of three independent experiments, each conducted in duplicate, +++ = p <0.001 compared to DMSO control; *** = p <0.001 compared to LPS control; ## = p <0.01 ethanolic extract compared to hot aqueous extract.

Fig. 5 in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways

Fig. 5. Effect of S. frutescens on A) CD86 (M1) and B) CD206 (M2) expression in LPS-induced RAW 264.7 cells. Data represent mean ± SEM of three independent experiments, each conducted in triplicate++ = p <0.05, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract.

Fig. 3. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways

Fig. 3. S. frutescens modulated the expression of pro-inflammatory cytokines in LPS-induced Raw 264.7 cells. Data represent mean ± SEM of three independent experiments, ++ = p <0.05, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared with LPS control; ### = p <0.001 ethanolic extract compared to hot aqueous extract.

Fig. 1. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways

Fig. 1. S.frutescens reduces (A) NO and (B) ROS production in LPS-induced RAW 264.7 cells. 10 μM Curcumin was used as an anti-inflammatory control. Data represent mean ± SEM of three independent experiments,each conducted in triplicate, +++ = p <0.001 compared to DMSO control; * = p <0.05,** = p <0.01,*** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract.

Fig. 2. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways

Fig. 2. S. frutescens reduces (A) iNOS and (B) COX-2 expression in LPS-induced RAW 264.7 cells. Data represent mean ± SEM of three independent experiments, each conducted in triplicate, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract.

Fig. 6. S in Regulation of M1/M2 macrophage polarization by Sutherlandia frutescens via NFkB and MAPK signaling pathways

Fig. 6. S. frutescens inhibits NFKB and MAPK activation in LPS-induced RAW 264.7 cells. (A) Quantitative analysis of nuclear NFKB localization determined using the cell scoring analysis module on MetaXpress® software (Version 5.1) of the ImageXpress Micro XLS (Molecular Devices, USA), with expression recorded as percentage of LPS induction. Changes in phosphorylation level of (B) p-p65 NFKB, (C) p-ERK 1/2 and (D) p-p38 detected by flow cytometry analysis, with expression recoded as percentage of untreated control. Data represents mean ± SEM of three independent experiments, each conducted in triplicate, +++ = p <0.001 compared to DMSO control; * = p <0.05, ** = p <0.01, *** = p <0.001 compared to LPS control; # = p <0.05 ethanolic extract compared to hot aqueous extract. Corresponding immunofluorescence images of control cells (Ei), LPS induced cells (Eii), LPS induced cells co-treated with 50 μg mL −1 and 200 μg mL −1 hot aqueous S. frutescens extract (Eiii, Eiv), LPS induced cells co-treated with 50 μg mL −1 and 200 μg mL −1 ethanolic S. frutescens extract (Ev, Evi). Cells were stained with anti-p65 NFKB antibody and co-stained with DAPI. Increased nuclear localization and increased cytoplasmic expression is seen following LPS treatment (Eii), which is reduced following co-treatment with S. frutescens (Eiii-Evi). Scale bar = 50 μm.

opencc-zeroOct 2022View details →

dryad32/100

Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM

Macrophages (ΜΦs) are important immune effector cells that promote (M1 ΜΦs) or inhibit (M2 ΜΦs) inflammation and are involved in numerous physiological and pathogenic immune responses. Their precise role and relevance, however, are not fully understood for lack of non-invasive quantification methods. Here, we show that two-photon excited fluorescence lifetime imaging (TPE-FLIM), a label-free non-invasive method, can visualize ΜΦs in the human dermis in vivo. We demonstrate in vitro that human dermal ΜΦs exhibit specific TPE-FLIM properties that distinguish them from the main components of the extracellular matrix and other dermal cells. We visualized ΜΦs, their phenotypes and phagocytosis in the skin of healthy individuals in vivo using TPE-FLIM. Additionally, machine learning identified M1 and M2 MФs with a sensitivity of 0.88±0.04 and 0.82±0.03 and a specificity of 0.89±0.03 and 0.90±0.03, respectively. In clinical research, TPE-FLIM can advance the understanding of the role of MФs in health and disease.

opencc-by-4.0Dec 2022View details →

Data storage for AOs exhibiting macrophage- and lysosome-like functions

Raw and processed data storage for study on artifical organelles which are able to mimick or to imitate macrophage- and lysosome-like functions. Integration of trypsin in two different pH-responsive polymersomes. Data storage for manuscript submission "Artificial organelles: Lysosome- or macrophage-like functions by pH- and salt-driven membrane permeability of trypsin loaded polymersomes".

opennotspecifiedNov 2017View details →

FIG. 3 in A potent anti-inflammatory response in bat macrophages may be linked to extended longevity and viral tolerance

FIG. 3. Bat-specific sites under positive selection in the investigated immune genes. Sites that had statistically significant evidence of positive selection acting across eutherian mammals were investigated for any amino acid substitutions unique to the ancestral bat lineage, the vespertilionid lineage or the pteropodid lineage. Bat lineages-specific sites are highlighted in yellow

opennotspecifiedNov 2017View details →

FIG. 2 in A potent anti-inflammatory response in bat macrophages may be linked to extended longevity and viral tolerance

FIG. 2. Signatures of adaptive evolution in genes involved in sensing of viral infection and activation of antiviral/proinflammatory signalling. Genes under positive selection (site-test) in mammals are highlighted in green. Genes with sites under positive selection in bat lineages are indicated with an asterix

opennotspecifiedNov 2017View details →

FIG. 1. Myotis myotis and M in A potent anti-inflammatory response in bat macrophages may be linked to extended longevity and viral tolerance

FIG. 1. Myotis myotis and M. musculus macrophages present differential response to LPS and Poly(I:C) challenge. Change in gene expression of A — IFN-β, B — IL-1β, C — TNF, D — Il-10 in response to LPS and Poly(I:C) treatment at 1, 4 and 24 hr time points are presented as changes in delta Ct values normalized to actin-β. Ratios of the fold induction of anti-inflammatory Il-10 to proinflammatory TNF (E) and Il-1β (F) are also presented. G — Literature derived Il-10/TNF ratios for other mammals. H — NO generated by stimulated M. myotis and mouse macrophages. Bars represent the mean of two experimental repeats ± SEM. Presented P -values indicate the significance of the difference between species from analysis of variance (ANOVA)

opencc-by-4.0Jul 2023View details →

18, 28, 42 DPI neuron and macrophage quantifications

MHC-II (-/-) mice expressing HLA DRB1*15:01 were immunized with a-synuclein32-46 peptide in complete Freund's adjuvant (CFA), CFA alone, or PBS alone. Enteric neuron density (ANNA1+), tyrosine hydroxylase (TH) neuron density, TH+ area and mean fluorescence intensity, and macrophage density (IBA1+) were quantified from the submucosal plexus and myenteric plexus of mice at 18, 28, and 42 days post immunization. These values were normalized to the CFA-only condition.

opencc-by-4.0Jul 2023View details →

Enteric neuron and macrophage quantification following T cell depletion

MHC-II (-/-) mice expressing HLA DRB1*15:01 were immunized with a-synuclein32-46. Data includes three conditions: isotype control, anti-CD4, and anti-CD8 T cell depleting antibodies. Enteric neuron (ANNA1+), macrophage density (IBA1+), tyrosine hydroxylase (TH+) neuron, TH+ area and mean fluorescence intensity in the myenteric and submucosal plexus of the ileum were quantified.

opencc-by-4.0Jul 2023View details →

Novel M2-like tumor-associated macrophage-related biomarkers predict prognosis of Acute myeloid leukemia patients.

The supplementary material for the Novel M2-like tumor-associated macrophage-related biomarkers predict prognosis of Acute myeloid leukemia patients.

Fig. 6 in Undescribed ecdysteroids and phenolic glycosides from the roots of Cyathula officinalis Kuan and their anti-inflammatory activity in LPS-induced RAW 264.7 macrophages in vitro

Fig. 6. Effects of 1 on iNOS, COX-2, and NF-κB protein expression in LPS-induced RAW 264.7 cells in vitro. "Dexamethasone" represents the positive control group, and "Control" represents the blank control, which was not treated with LPS or the tested compounds. (A) Protein expression of iNOS, COX-2, P65 and P–P65 was determined by Western blotting. (B) Relative protein expression of P–P65 to P65 is shown as a histogram. (C) Protein expression of iNOS and COX-2 relative to the control is shown as a histogram. The experiment was repeated three times. Data are presented as the mean ± SD. *P <0.05 vs. the LPS group, **P <0.01 vs. the LPS group.

Fig. 3 in Undescribed ecdysteroids and phenolic glycosides from the roots of Cyathula officinalis Kuan and their anti-inflammatory activity in LPS-induced RAW 264.7 macrophages in vitro

Fig. 3. NMR calculation results of two plausible stereoisomers of 1 at the B3LYP-D3 (BJ)/6-31G** level.

Fig. 5 in Undescribed ecdysteroids and phenolic glycosides from the roots of Cyathula officinalis Kuan and their anti-inflammatory activity in LPS-induced RAW 264.7 macrophages in vitro

Fig. 5. Effects of different concentrations (10, 20, 40 μM)) of 1 on LPS-stimulated release of (A) TNF-α, (B) IL-6, and (C) IL-1β in RAW 264.7 cells. The values represent the mean ± SD of three independent experiments, and differences between the mean values were assessed by Student's t-test. ##P <0.01 vs. the control group, *P <0.05 vs. the LPS group, **P <0.01 vs. the LPS group.

Fig. 4 in Undescribed ecdysteroids and phenolic glycosides from the roots of Cyathula officinalis Kuan and their anti-inflammatory activity in LPS-induced RAW 264.7 macrophages in vitro

Fig. 4. Effect of 1–6 (40 μM) on NO release in LPS-induced RAW 264.7 cells in vitro. Dexamethasone was used as a positive control in this experiment, and the concentration was 5 μM. The data represent the mean ± SD of three independent experiments.