Find research datasets worth reusing

opencc-zeroDec 2016View details →

Data from: The magnitude of ivacaftor effects on fluid secretion via R117H-CFTR channels: human in vivo measurements

We optically measured effects of orally available ivacaftor (Kalydeco®) on sweat rates of identified glands in 3 R117H subjects, each having a unique set of additional mutations, and compared them with 5 healthy control subjects tested contemporaneously. We injected β-adrenergic agonists intradermally to stimulate CFTR-dependent 'C-sweat' and methacholine to stimulate 'M-sweat', which persists in CF subjects. We focused on an R117H-7T/F508del subject who produced quantifiable C-sweat off ivacaftor and was available for 1 blinded, 3 off ivacaftor, and 3 on ivacaftor tests, allowing us to estimate in vivo fold-increase in sweat rates produced by ivacaftor's effect on the open probability (PO) of R117H-CFTR. Measured sweat rates must be corrected for sweat losses. With estimated sweat losses of 0.023 to 0.08 nl·gland-1·min-1, ivacaftor increased the average C-sweat rates 3-7 fold, and estimated function as % of WT were 4.1-12% off ivacaftor and 21.9-32% on ivacaftor (larger values reflect increased loss estimates). Based on single tests, an R117H-7T/ R117H-7T subject showed 6-9% WT function off ivacaftor and 28-43% on ivacaftor. Repeat testing of an R117H-5T/F508del subject detected only trace responding to ivacaftor. We conclude that in vivo, R117H PO is strongly increased by ivacaftor, but channel number, mainly determined by variable deletion of exon 10, has a marked influence on outcomes.

opencc-zeroDec 2011View details →

Data from: Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus

How epistatic interactions between mutations determine the genetic architecture of fitness is of central importance in evolution. The study of epistasis is particularly interesting for RNA viruses because of their genomic compactness, lack of genetic redundancy, and apparent low complexity. Moreover, interactions between mutations in viral genomes determine traits such as resistance to antiviral drugs, virulence and host range. In this study we generated 53 Tobacco etch potyvirus genotypes carrying pairs of single-nucleotide substitutions and measured their separated and combined deleterious fitness effects. We found that up to 38% of pairs had significant epistasis for fitness, including both positive and negative deviations from the null hypothesis of multiplicative effects. Interestingly, the sign of epistasis was correlated with viral protein–protein interactions in a model network, being predominantly positive between linked pairs of proteins and negative between unlinked ones. Furthermore, 55% of significant interactions were cases of reciprocal sign epistasis (RSE), indicating that adaptive landscapes for RNA viruses maybe highly rugged. Finally, we found that the magnitude of epistasis correlated negatively with the average effect of mutations. Overall, our results are in good agreement to those previously reported for other viruses and further consolidate the view that positive epistasis is the norm for small and compact genomes that lack genetic robustness.

opencc-zeroDec 2018View details →

Data from: Litter carbon and nutrient chemistry control the magnitude of soil priming effect

1. Plant litter inputs can promote the decomposition of soil organic matter (OM) through the priming effect (PE). However, whereas leaf litter chemistry has long been identified as the primary driver of litter decomposition within biomes worldwide, little is known about how litter chemical traits influence the occurrence and strength of the PE. 2. Here, we studied the effects of 15 co-occurring C3 leaf litters of contrasting chemistry on C4 soil respiration by analyzing changes in 13C natural abundance during early and later stages of litter decomposition (up to 125 days). 3. Besides an apparent PE of 16% in the first three days, soil C respiration was increased by 24% on average with leaf litter addition in the initial stage of decomposition (426 d) and by 8% at later stages (27125 d). Most interestingly, soil PE related well to initial litter chemistry and the dominant factors influencing the magnitude of the PE changed with decomposition stage. In the early stage of decomposition, litter leachate C content and litter hemicellulose concentration were positively correlated with the strength of the PE, whereas tannin concentration was negatively associated with soil PE. Together, tannin and hemicellulose explained half of the observed variation in the PE (R2 = 0.58). In the later phase of decomposition, lignin and lignin:N ratios were negatively related to the PE, whereas Ca, K and Mg concentrations were positively related to the PE; lignin alone gave the best prediction of the PE (R2 = 0.58) at later decomposition stages. 4. Our findings provide evidence that the magnitude and direction of the PE is influenced by the chemistry of organic matter inputs and suggest that, as decomposition proceeds differently among litter of contrasting chemistry, litters can also have variable effect on soil PE through time. The predictive power of litter chemical traits on soil PE opens new perspectives for improving our mechanistic understanding of soil PE and improving our abilities to model soil C dynamics at variable scales.

opencc-zeroDec 2017View details →

Data from: Environmental stress increases the magnitude of nonadditive genetic variation in offspring fitness in the frog Crinia georgiana

When organisms encounter heterogeneous environments, selection may favor the ability of individuals to tailor their phenotypes to suit the prevailing conditions. Understanding the genetic basis of plastic responses is therefore vital for predicting whether susceptible populations can adapt and persist under new selection pressures. Here, we investigated whether there is potential for adaptive plasticity in development time in the quacking frog Crinia georgiana, a species experiencing a drying climate. Using a North Carolina II breeding design, we exposed 90 family groups to two water depth treatments (baseline and low-water) late in larval development. We estimated the contribution of additive and non-additive sources of genetic variation to early offspring fitness under both environments. Our results revealed a marked decline in larval fitness under the stressful (low-water) rearing environment, but also that additive genetic variation was negligible for all traits. However, in most cases we found significant sire-by-dam interactions, indicating the importance of non-additive genetic variation for offspring fitness. Moreover, sire-by-dam interactions were modified by the treatment, indicating that patterns of non-additive genetic variance depend on environmental context. For all traits, we found higher levels of non-additive genetic variation (relative to total phenotypic variation) when larvae were reared under stressful conditions, suggesting that the fitness costs associated with incompatible parental crosses (e.g. homozygous deleterious recessive alleles) will only be expressed when water availability is low. Taken together, our results highlight the need to consider patterns of non-additive genetic variation under contrasting selective regimes when considering the resilience of species to environmental change.

openJan 2022View details →

Dataset: Implicit Learning of Parity and Magnitude Associations with Number Color

Dataset for accepted manuscript. SAV files for SPSS or open-source PSPP (https://www.gnu.org/software/pspp/). Behavioral data (accuracy and response time) for all 54 participants (human adults) included in the sample. Separate datasets for the parity and magnitude experiments. Variables: <ul> <li>Subj = randomized subject identification number</li> <li>Group = Experimental (category-level) or control (item-level) <ul> <li>Group 1 = Control</li> <li>Group 2 = Experimental</li> </ul> </li> <li>Acc_Inc = Accuracy (proportion correct) for incongruent (low-probability) trials</li> <li>Acc_Con = Accuracy (proportion correct) for congruent (high-probability) trials</li> <li>Acc_Effect = Congruent minus incongruent (%)</li> <li>RT_Con = Response time (s) for congruent (high-probability) trials</li> <li>RT_Inc = Response time (s) for incongruent (low-probability) trials</li> <li>RT_Effect = Incongruent minus congruent (ms)</li> <li>DD_Acc = Explicit association report task (with double-digits) accuracy (proportion correct)</li> <li>DD_RT = Explicit association report task (with double-digits) response time (s)</li> <li>TTR = Tempo-Test Rekenen score of mathematical fluency; score from 0-200</li> <li>Data across 5 experimental blocks <ul> <li>Acc_Inc_1 ... Acc_Inc_5 = Accuracy (proportion correct) for incongruent (low-probability) trials</li> <li>Acc_Con_1 ... Acc_Con_5 = Accuracy (proportion correct) for congruent (high-probability) trials</li> <li>RT_Inc_1 ... RT_Inc_5 = Response time (s) for incongruent (low-probability) trials</li> <li>RT_Con_1 ... RT_Con_5 = Response time (s) for congruent (high-probability) trials</li> </ul> </li> </ul>

opencc-by-4.0May 2024View details →

Attenuated sex-related DNA methylation differences in cancer highlight the magnitude bias mediating existing disparities

This is supplementary data for the manuscript, "Attenuated sex-related DNA methylation differences in cancer highlight the magnitude bias mediating existing disparities"

opencc-by-4.0Nov 2024View details →

Dynamic weakening in sandstone-derived fault gouges during simulated small-magnitude earthquakes under variable loading and environmental conditions

Faults exhibit dynamic weakening during large displacements (>1 m) at seismic slip velocities (>0.1 m/s), but the role of this weakening in small-displacement induced earthquakes (M 3–4), such as those in the Groningen Gas Field (the Netherlands), remains unclear. We conducted seismic slip-pulse experiments on Slochteren sandstone gouges (SSG) using a rotary-shear apparatus to investigate their dynamic behavior. Pre-sheared gouge layers, confined between ~1.5 mm thick sandstone host blocks, were subjected to slip pulses at initial effective normal stresses of 4.9–16.6 MPa and pore fluid pressures of 0.1 and 1 MPa under undrained conditions. Slip pulses reached peak velocities of 1.8 m/s, accelerations up to 42 m/s², and displacements of 7.5–15 cm, using either dry Argon or water as pore fluid at ambient temperatures. Water-saturated gouges showed rapid weakening from a peak friction of ~0.7 to ~0.3, with early dilatancy followed by slower ongoing dilation. In contrast, Argon-filled samples exhibited only subtle weakening. Our findings confirm that water-saturated SSG weakens substantially during slip, with minimal dependence on normal stress, slip acceleration, or displacement, while dry samples do not. Microstructural analysis indicates no systematic relationship between PSZ width and frictional work or power input densities, suggesting that wear or heat production alone does not govern PSZ growth. Instead, thermal pore fluid pressurization, potentially involving water phase transitions at asperity scales, may drive weakening in short-displacement, induced seismic events.

opencc-by-4.0Nov 2022View details →

From greenhouse to coolhouse: timing and magnitude of the first Cretaceous cooling event

LA-ICP-MS U-Pb dating results trace elements, stable isotope, and clumped isotope results

restrictedIPD-UNDECIDEDFeb 2026View details →

The Magnitude of Effect of PCP Counseling on Participation Rate and Sedation Rate in Colonoscopy-based Screening Program

ClinicalTrials.gov study NCT01688817. IPD Sharing: Not stated. Countries: 0. Publications: 1.

restrictedIPD-UNDECIDEDFeb 2026View details →

Bone Health in Pediatric Crohn's Disease: A Low Magnitude Mechanical Stimulus Trial

ClinicalTrials.gov study NCT00364130. IPD Sharing: Not stated. Countries: 1. Publications: 0.

closedIPD-NOFeb 2026View details →

Micromechanical Modeling Using Low Magnitude Mechanical Stimulation

ClinicalTrials.gov study NCT01921517. IPD Sharing: NO. Countries: 1. Publications: 0.

restrictedIPD-UNDECIDEDFeb 2026View details →

Safety and Efficacy Study of the Amaranth Medical MAGNITUDE Bioresorbable Drug-Eluting Coronary Stent (RENASCENT III)

ClinicalTrials.gov study NCT02900937. IPD Sharing: UNDECIDED. Countries: 2. Publications: 0.

controlledIPD-YESFeb 2026View details →

Flexible Attention to Magnitudes and Early Math

ClinicalTrials.gov study NCT05682729. IPD Sharing: YES. Countries: 1. Publications: 0.