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opennotspecifiedJul 2021View details →

Fig. 1 in MaNgROVIVIRga CUNICULI gen. nov., sp. nov., a moderately halophilic bacterium isolated from bioturbated Red Sea mangrove sediment, and proposal of the novel family MaNgROVIVIRgaCeae fam. nov.

Fig. 1. (a) Sediments in the mangrove forest at KAUST bioturbated by Uca species crabs; crab barrows are indicated by yellow arrows. Leaves forming mangrove litter are also visible. Bar, 10 cm. (b) Aquarium filled with mangrove bioturbated sediments and FSW for the incubation of DCs; bar, 6 cm. (c) Aerial view of DCs placed on the surface of mangrove sediments and cover with FSW inside the aquarium; bar, 6 cm.

FIGURE 9 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 9. Some goby-associated species of Alpheus mentioned in the text, photographed in situ: A—Alpheus thompsoni Anker, 2022 [colour pattern type A], Sulawesi, Indonesia; B, C—Alpheus aff. thompsoni Anker, 2022 [colour pattern type C] associated with Amblyeleotris periophthalma (Bleeker, 1853) in Bali, Indonesia; D—Alpheus cf. mannarensis Purushothaman, Abhilash, Kumar & Lal, 2021 [colour pattern type D] associated with Amblyeleotris sp. in Papua New Guinea; E, F—Alpheus sp. [colour pattern type E] associated with Amblyeleotris sungami (Klausewitz, 1969) in Sharm-El-Sheikh, Egypt. See text for further details. Photographs by Andrey Ryanskiy [A, B, D–F] and Scott and Jeanette Johnson [C].

FIGURE 5 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 5. Alpheus aff. karplusi, shrimps with their goby partners photographed in situ: A—shrimp associated with Lotilia klausewitzi Shibukawa, Hirata & Senou, 2012 in Romblon, Philippines; B, C—shrimp associated with L. klausewitzi in Kwajalein, Marshall Islands; D—shrimp associated with Cryptocentrus albidorsus (Yanagisawa, 1978) in Dongbei Jiao, Taiwan; E—shrimp associated with Cryptocentrus malindiensis (Smith, 1959) in Trou aux Biches, Mauritius; F—shrimp associated with Cryptocentrus leucostictus (Günther, 1872) in Bali, Indonesia. See text for further details. Photographs by Rickard Zerpe [A], Scott and Jeanette Johnson [B, C], Jimmy Cheng [D], Charlene Stenton-Dozey / Dive Spirit Mauritius [E] and Vincent Chalias [F].

FIGURE 6. Alpheus thompsoni Anker, 2022 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 6. Alpheus thompsoni Anker, 2022: male (cl 10.0 mm) from the Farasan Islands, Saudi Arabia (FLMNH UF 37002): A—frontal region, dorsal; B—same, lateral; C—telson, dorsal (plumose setae omitted); D—tooth on ventromesial carina of first article of antennular peduncle, lateral; E—third maxilliped, lateral; F—second pleopod, detail of appendix masculina and appendix interna; G—uropod, dorsal (plumose setae omitted); H—same, detail of spiniform setae on distal margin of endopod (plumose setae omitted).

FIGURE 7. Alpheus thompsoni Anker, 2022 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 7. Alpheus thompsoni Anker, 2022: male (cl 10.0 mm) from the Farasan Islands, Saudi Arabia (FLMNH UF 37002): A—major (right) cheliped, mesial; B—same, carpus and chela, lateral; C—same, dactylus, lateral (setae omitted); D—minor (left) cheliped, mesial; E—same, carpus and chela, lateral; F—same, ischium, merus and carpus, lateral (setae omitted); G—second pereiopod, lateral; H—third pereiopod, lateral; I—same, propodus and dactylus, ventrolateral; J—same, dactylus, ventral (setae on propodus omitted); K—fourth pereiopod, propodus and dactylus; L—fifth pereiopod, lateral; M—same, propodus and dactylus, lateral.

FIGURE 2 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 2. Alpheus karplusi sp. nov.: paratype male (cl 11.4 mm) (FLMNH UF 37016) [A–C, F–K] and holotype male (cl 10.5 mm) (FLMNH UF 37011) [D, E], both from Farasan Islands, Saudi Arabia; A—major (right) cheliped, lateral; B—same, chela, mesial; C—same, ischium, merus and carpus, mesial; D—minor (right) cheliped, chela and carpus, lateral; E, same, ischium, merus and carpus, lateral; F—second pereiopod, lateral; G—third pereiopod, lateral; H—same, propodus and dactylus, lateral; I—same, dactylus, ventral (setae omitted); J—fifth pereiopod, lateral; K—same, propodus and dactylus, mesial.

FIGURE 1 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 1. Alpheus karplusi sp. nov.: paratype male (cl 11.4 mm) from Farasan Islands, Saudi Arabia (FLMNH UF 37016); A—frontal region, dorsal; B—same, lateral; C—telson, dorsal (plumose setae omitted); D—tooth on ventromesial carina of first article of antennular peduncle, lateral; E—third maxilliped, lateral; F—second pleopod, detail of appendix masculina and appendix interna, anterior (lateral); G—uropod, dorsal (plumose setae omitted); H—same, detail of spiniform setae on distal margin of endopod (plumose setae omitted).

FIGURE 3 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 3. Alpheus karplusi sp. nov., shrimp in life: A, B—paratype female (cl 7.4 mm) from Thuwal, Saudi Arabia (FLMNH UF 37070), general dorsal (A) and lateral (B) views. Photographs by the author.

FIGURE 8. Alpheus thompsoni Anker, 2022 in On two goby-associated snapping shrimps from the Red Sea, one of them new to science (Malacostraca: Decapoda: Alpheidae: Alpheus)

FIGURE 8. Alpheus thompsoni Anker, 2022, shrimps in life, colour pattern type B (see text for details): A, B—male (cl 10.0 mm) from the Farasan Islands, Saudi Arabia (FLMNH UF 37002), general dorsal view (A) and detail of frontal region (B); C—male from Kenting, Taiwan (deposited in Academia Sinica, Taipei, specimen examined by the author in 2008), general dorsal view; D—shrimp in situ in association with the goby Amblyeleotris sp., Kwajalein, Marshall Islands. Photographs by the author [A, B], Dr. Ming-Shiou Jeng [C] and Scott and Jeanette Johnson [D].

FIGURE 2 in Cynosurus peltieri (Pooideae, Poaceae), a poorly known species endemic to the Algerian-Tunisian terrestrial flora: further insights on its occurrence, IUCN Red List assessment, and lectotypification

FIGURE 2. Cynosurus polybracteatus in its habitat in Kroumirian area. A. Habit of the plant. B–C. Details of its unilateral and linear, indistinctly lobed panicle, with very short branches. All photographs were taken by Ridha El Mokni in Tabarka (Jendouba, NW of Tunisia, North Africa), 14 May 2013 and 3 May 2017.

FIGURE 1 in Cynosurus peltieri (Pooideae, Poaceae), a poorly known species endemic to the Algerian-Tunisian terrestrial flora: further insights on its occurrence, IUCN Red List assessment, and lectotypification

FIGURE 1. Cynosurus peltieri in its typical habitat in Kroumirian oak forests. A. Habit of the plant. B. Detail of its unilateral and linear, clearly lobed panicle. All photographs were taken by Ridha El Mokni in Aîn Zana-Aîn Draham (Jendouba, NW of Tunisia, North Africa), 16 June 2020.

opencc-by-4.0Feb 2022View details →

Raw gel and membrane figures associated with the publication "Iron-loaded deferiprone can support full hemoglobinization of cultured red blood cells"

Raw gel and membrane figures associated with the publication Iron-loaded deferiprone can support full hemoglobinization of cultured red blood cells.   For Figure 1a: Raw uncropped image of the gel run with samples combining apotransferrin with iron chelators. After staining with Coomassie, gel was scanned in an Epson V500 scanner (full color, 1200 dpi, bit depth = 24).   For Figure 3: Raw uncropped images of the membranes obtained from western blots of cultured erythroblasts derived from three different donors, showing the expression level of proteins involved in iron metabolism regulation (ferritin, transferrin receptor, total EIF2, phospho-EIF2). Actin was used as housekeeping protein for relative quantification of protein abundance for further analysis. After staining, membranes were scanned in an Epson V500 scanner (full color, 1200 dpi, bit depth = 24). For all membranes, the loading order is the same: - Day 0: 1000 μg/mL holotransferrin - Day 0: 100 μg/mL holotransferrin - Day 0: 100 μg/mL holotransferrin + 52 μM iron-loaded deferiprone - Day 1: 52 μM iron-loaded deferiprone - Day 1: 1000 μg/mL apotransferrin - (empty lane) - Day 1: 1000 μg/mL holotransferrin - Day 1: 100 μg/mL holotransferrin - Day 1: 100 μg/mL holotransferrin + 52 μM iron-loaded deferiprone - Day 1: 52 μM iron-loaded deferiprone - Day 1: 1000 μg/mL apotransferrin - (empty lane) - Day 2: 1000 μg/mL holotransferrin - Day 2: 100 μg/mL holotransferrin - Day 2: 100 μg/mL holotransferrin + 52 μM iron-loaded deferiprone - Day 2: 52 μM iron-loaded deferiprone - Day 2: 1000 μg/mL apotransferrin More details on the protocol for western blot available in the manuscript at https://doi.org/10.1101/2021.08.02.454758   For Figure 4: Raw uncropped images of the membranes obtained from western blots of cultured erythroblasts derived from two different donors with different iron supplementations (no hTf or Def, 52 μM iron-loaded deferiprone, or 300 μg/mL holotransferrin), showing the phosphorylation level of STAT5 upon withdrawal of erythropoietin (Epo) (3 hours) and restimulation with 0.2 or 1.0 U/mL Epo. After staining, membranes were scanned in an Epson V500 scanner (full color, 1200 dpi, bit depth = 24). For both membranes, the loading order is the same: - Lane 1: cells cultured without hTf nor Def, and no Epo restimulation - Lane 2: cells cultured with 52 μM iron-loaded deferiprone, and no Epo restimulation - Lane 3: cells cultured with 300 μg/mL holotransferrin, and no Epo restimulation - Lane 4: cells cultured without hTf nor Def, and restimulation with 0.2 U/mL Epo. - Lane 5: cells cultured with 52 μM iron-loaded deferiprone, and restimulation with 0.2 U/mL Epo. - Lane 6: cells cultured with 300 μg/mL holotransferrin, and restimulation with 0.2 U/mL Epo. - Lane 7: cells cultured without hTf nor Def, and restimulation with 1.0 U/mL Epo. - Lane 8: cells cultured with 52 μM iron-loaded deferiprone, and restimulation with 1.0 U/mL Epo. - Lane 9: cells cultured with 300 μg/mL holotransferrin, and restimulation with 1.0 U/mL Epo.

Figure 8 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 8. Potential refugial areas of different mtDNA haplogroups of Cervus elaphus and their post-LGM migration routes hypothesised based on the results of this study. The northern limit of the range of C. elaphus during the LGM is determined based on the study of Niedziałkowska et al. (2021). C. elaphus records are listed in the Supporting Information, Tables S1, S2.

Figure 7 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 7. Distribution of mtDNA red deer s.l. haplogroups in Eurasia after the LGM. Upper panel, between 18 and 9 kya; Middle panel, between 9 and 4 kya; Lower panel, between 4 kya and today. The colours of samples correspond to haplogroups as in previous figures. Red deer s.l. records are listed in the Supporting Information, Tables S1, S2.

Figure 6 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 6. Distribution of mtDNA red deer s.l. haplogroups in Eurasia before the LGM. Upper panel, in the period older than 41 kya; Lower panel, between 41 and 26 kya. The colours of samples correspond to haplogroups as in previous figures. Red deer s.l. records are listed in the Supporting Information, Tables S1, S2.

Figure 5 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 5. Dated phylogeny of the elaphoid deer obtained in BEAST from radiocarbon-dated ancient sequences and randomly chosen modern sequences. Black circles at nodes indicate posterior probabilities above 0.7 and red circles posterior probabilities above 0.9. Rectangles at nodes represent 95% HPD of selected node ages. The graph below the phylogeny shows temperature change according to data from the Vostock ice core (Petit et al., 1999).

Figure 4 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 4. Dated phylogeny of the elaphoid and wapitoid deer obtained in BEAST from radiocarbon-dated ancient sequences and randomly chosen modern sequences, displayed in the context of temperature change. Black circles at nodes indicate posterior probabilities above 0.7. and red circles posterior probabilities above 0.9. Purple rectangles at well-supported nodes represent 95% HPD of estimated ages. For better resolution, the deepest node interval is not fully shown. Ancient samples placed outside haplogroups are described by sample name, geographic location and median radiocarbon age. The graph below the phylogeny shows temperature oscillations according to data from the Vostock ice core (Petit et al., 1999).

Figure 3 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 3. Median-joining haplotype network calculated with PopART. Black circles represent missing haplotypes. Hatch marks represent mutational steps between haplotypes. Haplotype names and haplogroup colours correspond to those in the Bayesian tree (Fig. 2).

Figure 2 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 2. Bayesian phylogeny of Cytb haplotypes (1131 bp) from ancient and modern specimens calculated with MrBayes. Black circles at nodes indicate posterior probabilities above 0.7 and red circles posterior probabilities above 0.9. Names of haplotypes occurring only in modern samples are represented by haplotype names in bold, whereas those occurring in both modern and ancient samples are represented by italicized names, and those found only in ancient samples are in normal font. Names in bigger grey or black font indicate intermediate ancient haplotypes from elaphoid and wapitoid clades, respectively, that do not belong to any of the distinguished haplogroups. Detailed descriptions of haplotypes are presented in the Supporting Information, Tables S1, S2.

Figure 1 in Phylogenetics and phylogeography of red deer mtDNA lineages during the last 50 000 years in Eurasia

Figure 1. Geographic distribution of ancient and historical red deer s.l. samples analysed in this study. Modern range of C. elaphus was according to Niedziałkowska et al. (2021) and references therein. Modern range of C. canadensis refers to its range in Asia only and was created based on IUCN data and data from Stepanova (2010). The localization of borders of the easternmost range of elaphoids and the westernmost range of wapitiods is based on the results of the present study and published data (Meiri et al., 2018).