Find research datasets worth reusing

Data from: Effects of host genetics and environment on egg-associated microbiotas in brown trout (Salmo trutta)

Recent studies found fish egg-specific bacterial communities that changed over the course of embryogenesis, suggesting an interaction between the developing host and its microbiota. Indeed, single-strain infections demonstrated that the virulence of opportunistic bacteria is influenced by environmental factors and host immune genes. However, the interplay between a fish embryo host and its microbiota has not been studied yet at the community level. To test whether host genetics affects the assemblage of egg-associated bacteria, adult brown trout (Salmo trutta) were sampled from a natural population. Their gametes were used for full-factorial in vitro fertilizations to separate sire from dam effects. In total, 2520 embryos were singly raised under experimental conditions that differently support microbial growth. High-throughput 16S rRNA amplicon sequencing was applied to characterize bacterial communities on milt and fertilized eggs across treatments. Dam and sire identity influenced embryo mortality, time until hatching and composition of egg-associated microbiotas, but no link between bacterial communities on milt and on fertilized eggs could be found. Elevated resources increased embryo mortality and modified bacterial communities with a shift in their putative functional potential. Resource availability did not significantly affect any parental effects on embryo performance. Sire identity affected bacterial diversity that turned out to be a significant predictor of hatching time: embryos associated with high bacterial diversity hatched later. We conclude that both host genetics and the availability of resources define diversity and composition of egg-associated bacterial communities that then affect the life history of their hosts.

Data from: Life on the edge: a comparative study of eco-physiological adaptations of frogs to tropical semiarid environments

A key goal of ecology and evolution is to understand the relative contributions of environment and history in determining the geographic distribution of organisms. For the Neotropical lowlands, where temperatures are similar across landscapes, we hypothesize that water balance may be a critical but understudied factor in determining the distribution of species. Amphibians are especially sensitive to variation in precipitation due to their permeable skin. Here we focused on lowland frogs of north-western South America and investigated variation among 17 species in potentially important ecologically relevant performance variables related to water balance, and tested for possible adaptations to semiarid conditions within species. We studied frogs from coastal xeric, savannah and wet forest biomes under common laboratory conditions and quantified rates of evaporative water loss, rates of water uptake, and variation in water-searching behavior and performance. We found significant differences in all three performance variables among species, even after accounting for shared evolutionary history. A phylogenetic ANCOVA showed that categorizing species by ecological habits (terrestrial vs. arboreal) explained much of the eco-performance trait variation among species. Secondarily, environment explained additional variation, e.g., coastal xeric species showed reduced rates of water loss and terrestrial savannah amphibians showed lower rates of water uptake. Conspecific frog populations from different biomes exhibited similar performance. We compare our results with previous studies and conclude that ecological habit is the principle factor that predicts eco-physiological trait variation and the possible geographic distribution of lowland Neotropical frogs.

opencc-zeroDec 2014View details →

Data from: Paternity analyses in wild-caught and lab-reared Caribbean cricket females reveal the influence of mating environment on post-copulatory sexual selection

Polyandry is ubiquitous in insects and provides the conditions necessary for male- and female-driven forms of post-copulatory sexual selection to arise. Populations of Amphiacusta sanctaecrucis exhibit significant divergence in portions of the male genitalia that are inserted directly into the female reproductive tract, suggesting that males may exercise some post-copulatory control over fertilization success. We examine the potential for male-male and male-female post-copulatory interactions to influence paternity in wild-caught females of A. sanctaecrucis and contrast our findings with those obtained from females reared in a high-density laboratory environment. We find that female A. sanctaecrucis exercise control by mating multiple times (females mount males), but that male-male post-copulatory interactions may influence paternity success. Moreover, post-copulatory interactions that affect reproductive success of males are not independent of mating environment: clutches of wild-caught females exhibit higher sire diversity and lower paternity skew than clutches of lab-reared females. There was no strong evidence for last-male precedence in either case. Most attempts at disentangling the contributions of male-male and male-female interactions toward post-copulatory sexual selection have been undertaken in a laboratory setting and may not capture the full context in which they take place —such as the relationship between pre-mating and post-mating interactions. Our results reinforce the importance of designing studies that can capture the multifaceted nature of sexual selection for elucidating the role of post-copulatory sexual selection in driving the evolution of male and female reproductive traits, especially when different components (e.g., pre-copulatory and post-copulatory interactions) do not exert independent effects on reproductive outcomes.

opencc-zeroDec 2014View details →

Data from: Extreme operative temperatures are better descriptors of the thermal environment than mean temperatures

In ecological studies of thermal biology the thermal environment is most frequently described using the mean or other measures of central tendency in environmental temperatures. However, this procedure may hide biologically relevant thermal variation for ectotherms, potentially misleading interpretations. Extremes of operative temperatures (EOT) can help with this problem by bracketing the thermal environment of focal animals. Within this paper, we quantify how mean operative temperatures relate to the range of simultaneously available operative temperatures (a measure of error). We also show how EOT: 1) detect more thermal differences among microsites than measures of central tendency, like the mean OT, 2) allow inferring on microsite use by ectothermic animals, and 3) clarify the relationships between field operative temperatures and temperatures measured at weather stations (WS). To do that, we explored operative temperatures measured at four sites of the Brazilian Caatingas and their correspondent nearest weather stations. We found that the daily mean OT can hide temperature ranges of 41 °C simultaneously available at our study sites. In addition, EOT detected more thermal differences among microsites than central quantiles. We also show how EOT allow inferring about microsite use of ectothermic animals in a given site. Finally, the daily maximum temperature and the daily temperature range measured at WSs predicted well the minimum available field OT at localities many kilometers away. Based on our results, we recommend the use of EOT, instead of mean OT, in thermal ecology studies.

Data from: Thirdhand smoke uptake to aerosol particles in the indoor environment

Aerosol composition measurements made in an indoor classroom indicate the uptake of thirdhand smoke (THS) species to indoor particles, a novel exposure route for THS to humans indoors. Chemical speciation of the organic aerosol fraction using mass spectrometric data and factor analysis identified a reduced nitrogen component, predominantly found in the indoor environment, contributing 29% of the indoor submicron aerosol mass. We identify this factor as THS compounds partitioning from interior surfaces to gas phase and then aerosol phase. Partitioning of THS vapors to aerosols requires an aqueous phase for reactive uptake of the reduced nitrogen species (RdNS), leading to seasonal differences in THS concentration indoors. RdNS protonate under the acidic conditions expected for indoor aerosols of outdoor origin. Controlled laboratory measurements performed using cigarette smoke deposited into a Pyrex vessel showed a similar partitioning behavior to aerosol of outdoor origin and mass spectral features comparable to the measured indoor THS factor after 1 week of residence time in the closed vessel. This study reports a new, potentially large THS exposure route from partitioning of surface volatile organic compounds into the aerosol phase and subsequent dispersion in a mechanically ventilated building.

opencc-zeroDec 2012View details →

Data from: Adaptation of diploid and tetraploid Chamerion angustifolium to elevation but not local environment

Polyploid organisms often have different geographic ranges than their diploid relatives. However, it is unclear whether this divergence is maintained by adaptation or results from historical differences in colonization. Here we conducted a reciprocal transplant experiment with diploid and autotetraploid Chamerion angustifolium to test for adaptation at the ploidy and population level. In the Rocky Mountains, pure diploid populations occur at high elevations and pure autotetraploid populations occur at low elevations with mixed-ploidy populations between. We planted 3134 seedlings in 2004 and 3890 juveniles (bolting) in 2005 among nine plots, three in each of the diploid, mixed-ploidy and tetraploid zones, and monitored survival until 2008. For both seedlings and juvenile plants, elevation significantly influenced survival. The juvenile plants also showed a significant ploidy by elevation interaction, indicating that diploids and tetraploids survived best at their native elevations. In contrast, we found no evidence of local adaptation to plot within elevation. This suggests that the current distribution of diploids and tetraploids across elevations is the result of adaptation and that genome duplication may have facilitated the invasion of lower elevation habitats by limiting the movement of maladapted alleles from diploid populations at higher elevations.

Data from: Fragile coexistence of a global chytrid pathogen with amphibian populations is mediated by environment and demography

Unravelling the multiple interacting drivers of host pathogen co-existence is crucial in understanding how an apparently stable state of endemism may shift towards an epidemic and lead to biodiversity loss. Here, we investigate the apparent co-existence of the global amphibian pathogen *Batrachochytrium dendrobatidis* (Bd) with *Bombina variegata* populations in the Netherlands over a seven-year period. We used a multi-season mark-recapture data set and assessed potential drivers of co-existence (individual condition, environmental mediation and demographic compensation) at the individual and population level. We show that even in a situation with a clear cost incurred by endemic Bd, population sizes remain largely stable. Current environmental conditions and an over-dispersed pathogen load likely stabilize disease dynamics, but as higher temperatures increase infection probability, changing environmental conditions, for example a climate change-driven rise in temperature, could unbalance the current fragile host-pathogen equilibrium. Understanding the proximate mechanisms of such environmental mediation and of site-specific differences in infection dynamics can provide vital information for mitigation actions.

Data from: Isolation by environment in the highly mobile olive ridley turtle (Lepidochelys olivacea) in the eastern Pacific

Spatial and temporal scales at which processes modulate genetic diversity over the landscape are usually overlooked, impacting the design of conservation management practices for widely distributed species. We examine processes shaping population divergence in highly mobile species by re-assessing the case of panmixia in the iconic olive ridley turtle from the eastern Pacific. We implemented a biophysical model of connectivity and a seascape genetic analysis based on nuclear DNA variation of 634 samples collected from 27 nesting areas. Two genetically distinct populations largely isolated during reproductive migrations and mating were detected, each composed of multiple nesting sites linked by high connectivity. This pattern was strongly associated with a steep environmental gradient and also influenced by ocean currents. These findings relate to meso-scales features of a dynamic oceanographic interface in the eastern tropical Pacific (ETP) region, a scenario that possibly provides different cost-benefit solutions and selective pressures for sea turtles during both the mating and migration periods. We reject panmixia and propose a new paradigm for olive ridley turtles where reproductive isolation due to assortative mating is linked to its environment. Our study demonstrates the relevance of integrative approaches for assessing the role of environmental gradients and oceanographic currents as drivers of genetic differentiation in widely distributed marine species. This is relevant for the conservation management of species of highly mobile behaviour; and assists the planning and development of large-scale conservation strategies for the threatened olive ridley turtles in the ETP.

opencc-zeroDec 2018View details →

Data from: How has the environment shaped geographical patterns of insect body sizes? A test of hypotheses using sphingid moths

Aim: We mapped the geographical pattern of body sizes in sphingid moths and investigated latitudinal clines. We tested hypotheses concerning their possible environmental control, i.e., effects of temperature (negative: temperature size rule or Bergmann's rule; positive: converse Bergmann rule), food availability, robustness to starvation during extreme weather, and seasonality. Location: Old World and Australia/Pacific region Methods: Body size data of 950 sphingid species were compiled and related to their distribution maps. Focusing on body length, we mapped the median and maximum size of all species occurring in 100 km grid cells. In a comparative approach we tested the predictions from explanatory hypotheses by correlating species' size to the average environmental conditions encountered throughout their range, under univariate and multivariate models. We accounted for phylogeny by stepwise inclusion of phylogenetically informed taxonomic classifications into hierarchical random-intercept mixed models. Results: Median body sizes showed a distinctive geographical pattern, with large species in the Middle East and the Asian tropics, and smaller species in temperate regions and the Afrotropics. Absolute latitude explained very little body size variation, but there was a latitudinal cline of maximum size. Species' median size was correlated to net primary productivity, supporting the food availability hypothesis, whereas support for other hypotheses was weak. Environmental correlations contributed much less (i.e., <10%) to explaining overall size variation than phylogeny (inclusion of which led to models explaining >70% of variability). Main conclusion: The intuitive impression of larger species in the tropics is shaped by larger size maxima. Median body sizes are only very weakly related to latitude. Most of the geographic variation in body size in sphingid moths is explained by their phylogenetic past. NPP and forest cover correlate positively with the body size, which supports the idea that food availability allowed the evolution of larger sizes.

Data from: Genetic variation in social environment construction influences the development of aggressive behavior in Drosophila melanogaster

Individuals are not merely subject to their social environments; they choose and create them, through a process called social environment (or social niche) construction. When genotypes differ in social environment-constructing behaviors, different genotypes are expected to experience different social environments. As social experience often affects behavioral development, quantitative genetics and psychology theories predict that genetic variation in social environment construction should have an important role in determining phenotypic variation; however, this hypothesis has not been tested directly. I identify multiple mechanisms of social environment construction that differ among natural genotypes of Drosophila melanogaster and investigate their consequences for the development of aggressive behavior. Male genotypes differed in the group sizes that they preferred and in their aggressive behavior; both of these behaviors influenced social experience, demonstrating that these behaviors function as social environment-constructing traits. Further, the effects of social experience—as determined in part by social environment construction—carried over to affect focal male aggression at a later time and with a new opponent. These results provide manipulative experimental support for longstanding hypotheses in psychology, that genetic variation in social environment construction has a causal role in behavioral development. More broadly, these results imply that studies of the genetic basis of complex traits should be expanded to include mechanisms by which genetic variation shapes the environments that individuals experience.

Data from: Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment

Effective marine management requires comprehensive data on the status of marine biodiversity. However, efficient methods that can document biodiversity in our oceans are currently lacking. Environmental DNA (eDNA) sourced from seawater offers a new avenue for investigating the biota in marine ecosystems. Here, we investigated the potential of eDNA to inform on the breadth of biodiversity present in a tropical marine environment. Directly sequencing eDNA from seawater using a shotgun approach resulted in only 0.34% of 22.3 million reads assigning to eukaryotes, highlighting the inefficiency of this method for assessing eukaryotic diversity. In contrast, using 'tree of life' (ToL) metabarcoding and 20-fold fewer sequencing reads, we could detect 287 families across the major divisions of eukaryotes. Our data also show that the best performing 'universal' PCR assay recovered only 44% of the eukaryotes identified across all assays, highlighting the need for multiple metabarcoding assays to catalogue biodiversity. Lastly, focusing on the fish genus Lethrinus, we recovered intra- and inter-specific haplotypes from seawater samples, illustrating that eDNA can be used to explore diversity beyond taxon identifications. Given the sensitivity and low cost of eDNA metabarcoding we advocate this approach be rapidly integrated into biomonitoring programs.

opencc-zeroJun 2019View details →

Data from: Phenotype-environment mismatch in metapopulations - implications for the maintenance of maladaptation at the regional scale

Maladaptation is widespread in natural populations. However, maladaptation has most often been associated with absolute population decline in local habitats rather than on a spectrum of relative fitness variation that can assist natural populations in their persistence at larger regional scales. We report results from a field experiment that tested for relative maladaptation between pond habitats with spatial heterogeneity and (a)symmetric selection in pH. In the experiment, we quantified relative maladaptation in a copepod metapopulation as a mismatch between the mean population phenotype and the optimal trait value that would maximize mean population fitness under either stable or fluctuating pH environmental conditions. To complement the field experiment, we constructed a metapopulation model that addressed both relative (distance from the optimum) and absolute (negative population growth) maladaptation, with the aim of forecasting maladaptation to pH at the regional scale in relation to spatial structure (environmental heterogeneity and connectivity) and temporal environmental fluctuations. The results from our experiment indicated that maladaptation to pH at the regional scale depended on the asymmetry of the fitness surface at the local level. The results from our metapopulation model revealed how dispersal and (a)symmetric selection can operate on the fitness surface to maintain maladaptive phenotype-environment mismatch at local and regional scales in a metapopulation. Environmental stochasticity resulted in the maintenance of maladaptation that was robust to dispersal, but also revealed an interaction between the asymmetry in selection and environmental correlation. Our findings emphasize the importance of maladaptation for planning conservation strategies that can support adaptive potential in fragmented and changing landscapes.

Data from: Adaptive patterns of phenotypic plasticity in laboratory and field environments in Drosophila melanogaster

Identifying mechanisms of adaptation to variable environments is essential in developing a comprehensive understanding of evolutionary dynamics in natural populations. Phenotypic plasticity allows for phenotypic change in response to changes in the environment, and as such may play a major role in adaptation to environmental heterogeneity. Here, the plasticity of stress response in D. melanogaster originating from two distinct geographic regions and ecological habitats was examined. Adults were given a short-term, 5-day exposure to combinations of temperature and photoperiod to elicit a plastic response for three fundamental aspects of stress tolerance that vary adaptively with geography. This was replicated in both the laboratory and in outdoor enclosures in the field. In the laboratory, geographic origin was the primary determinant of the stress response. Temperature and the interaction between temperature and photoperiod also significantly affected stress resistance. In the outdoor enclosures, plasticity was distinct among traits and between geographic regions. These results demonstrate that short-term exposure of adults to ecologically relevant environmental cues results in predictable effects on multiple aspects of fitness. These patterns of plasticity vary among traits and are highly distinct between the two examined geographic regions, consistent with patterns of local adaptation to climate and associated environmental parameters.

Data from: AudioMoth: evaluation of a smart open acoustic device for monitoring biodiversity and the environment

1. The cost, usability and power efficiency of available wildlife monitoring equipment currently inhibits full ground-level coverage of many natural systems. Developments over the last decade in technology, open science, and the sharing economy promise to bring global access to more versatile and more affordable monitoring tools, to improve coverage for conservation researchers and managers. 2. Here we describe the development and proof-of-concept of a low-cost, small-sized and low-energy acoustic detector: 'AudioMoth'. The device is open-source and programmable, with diverse applications for recording animal calls or human activity at sample rates of up to 384kHz. We briefly outline two ongoing real-world case studies of large-scale, long-term monitoring for biodiversity and exploitation of natural resources. These studies demonstrate the potential for AudioMoth to enable a substantial shift away from passive continuous recording by individual devices, towards smart detection by networks of devices flooding large and inaccessible ecosystems. 3. The case studies demonstrate one of the smart capabilities of AudioMoth, to trigger event logging on the basis of classification algorithms that identify specific acoustic events. An algorithm to trigger recordings of the New Forest cicada (Cicadetta montana) demonstrates the potential for AudioMoth to vastly improve the spatial and temporal coverage of surveys for the presence of cryptic animals. An algorithm for logging gunshot events has potential to identify a shotgun blast in tropical rainforest at distances of up to 500 m, extending to 1km with continuous recording. 4. AudioMoth is more energy efficient than currently available passive acoustic monitoring (PAM) devices, giving it considerably greater portability and longevity in the field with smaller batteries. At a build cost of ~US$43 per unit, AudioMoth has potential for varied applications in large-scale, long-term acoustic surveys. With continuing developments in smart, energy-efficient algorithms and diminishing component costs, we are approaching the milestone of local communities being able to afford to remotely monitor their own natural resources.

opencc-zeroDec 2011View details →

Data from: Fitness effects of thermoregulation in a thermally changing environment

To better understand the evolution of phenotypic plasticity and thermoregulation and their potential value for ectotherms in the face of global warming, we conducted field experiments to measure their effects on fitness and their association with reproductive phenology in Plantago lanceolata in a thermally variable environment. We measured the reproductive timing and success of genotypes varying in thermoregulation, as mediated by floral-reflectance plasticity. Results were consistent with the hypothesis that thermoregulation is more adaptive when thermally variable reproductive seasons are shorter and cooler. Strong thermoregulation/plasticity increased reproductive success during the cool portion of the reproductive season but not during the warm portion. Directional selection that favored strongly thermoregulating genotypes early in the season shifted to stabilizing selection that favored genotypes with weaker thermoregulation later in the season. Thermoregulation and reproductive phenology were negatively correlated. Although reproductive onset and duration were similar between genotypes, strong thermoregulators produced more and larger spikes (clutches) early; weak thermoregulators produced more spikes late. Results suggest that with atmospheric warming, the benefit of raising body temperature via thermoregulation when it is cool should decline in extant populations. The negative correlation between thermoregulation and phenology should accelerate the evolutionary shift toward thermoconformity, that is, reduced plasticity.

Data from: The importance of growing up: juvenile environment influences dispersal of individuals and their neighbours

Dispersal is a key ecological process that is strongly influenced by both phenotype and environment. Here, we show that juvenile environment influences dispersal not only by shaping individual phenotypes, but also by changing the phenotypes of neighbouring conspecifics, which influence how individuals disperse. We used a model system (Tribolium castaneum, red flour beetles) to test how the past environment of dispersing individuals and their neighbours influences how they disperse in their current environment. We found that individuals dispersed especially far when exposed to a poor environment as adults if their phenotype, or even one‐third of their neighbours' phenotypes, were shaped by a poor environment as juveniles. Juvenile environment therefore shapes dispersal both directly, by influencing phenotype, as well as indirectly, by influencing the external social environment. Thus, the juvenile environment of even a minority of individuals in a group can influence the dispersal of the entire group.

Data from: The pervasive effects of lighting environments on sensory drive in bluefin killifish: an investigation into male/male competition, female choice, and predation

Sensory drive predicts that the conditions under which signaling takes place have large effects on signals, sensory systems, and behavior. The coupling of an ecological genetics approach with sensory drive has been fruitful. An ecological genetics approach compares populations that experience different environments and asks whether population differences are adaptive and are the result of genetic and/or environmental variation. The multi-faceted effects of signaling environments are well-exemplified by the bluefin killifish. In this system, males with blue anal fins are abundant in tannin-stained swamps that lack UV/blue light but are absent in clear springs where UV/blue light is abundant. Past work indicates that lighting environments shape genetic and environmental variation in color patterns, visual systems, and behavior. Less is known about the selective forces creating the across population correlations between UV/blue light and the abundance of blue males. Here, we present three new experiments that investigate the roles of lighting environments on male competition, female mate choice, and predation. We found strong effects of lighting environments on male competition where blue males were more likely to emerge as dominant in tea-stained water than in clear water. Our preliminary study on predation indicated that blue males may be less susceptible to predation in tea-stained water than in clear water. However, there was little evidence for female preferences favoring blue males. The resulting pattern is one where the effects of lighting environments on genetic variation and phenotypic plasticity match the direction of selection and favor the expression of blue males in swamps.

Data from: Are 2D space-use analyses adapted to animals living in 3D environments? A case study on a fish shoal

Methodologies enabling the monitoring of animal movement and behavior in 3-dimensions (3D; x, y, z, the latter accounting for the vertical dimension) are becoming increasingly accessible and can be deployed on entire groups of animals inhabiting 3D habitats. When 2-dimensional (2D; x, y) space-use analyses are used on such groups, their spatial organization is represented as a planar projection of individuals' space-use. Movement on the vertical dimension is ignored and could biased ecological inference made from the spatial structure of the group. We used a digital imaging technique to track movements and feeding behavior of individual animals within a free-range aggregation of juvenile drift-feeding fish (Galaxias anomalus) in 3D and at fine spatiotemporal scales. We estimated spatiotemporal overlap of space-use and feeding territories between group-members using 2D (x, y) and 3D spatial analysis to: (1) describe the spatial structure of the group, (2) identify patterns of resource partitioning, and (3) investigate the relationship between space-use overlap and feeding behavior. We found that overlapping ratios of space-use and feeding territories were over-estimated in 2D, while 3D analysis of space-use provided evidence of spatial partitioning between group-members. We also found that, regardless of the computation used, the overlapping ratios of space-use were positively correlated with overlapping ratios of feeding territories while no effect was found on feeding activity. In conclusion, whilst 3D analysis provided valuable information on the spatial structure of a group, inferences on the ecological function of space-use can also be obtained from 2D analysis.

Data from: Genet dynamics of a regenerating dwarf bamboo population across heterogeneous light environments in a temperate forest understorey

Despite the advantage of plant clonality in patchy environments, studies focusing on genet demography in relation to spatially heterogeneous environments remain scarce. Regeneration of bamboos in forest understoreys after synchronous die-off provides an opportunity for assessing how they come to proliferate across heterogeneous light environments. In a Japanese forest, we examined genet demography of a population of Sasa kurilensis over a 7-year period starting 10 years after die-off, shortly after which some genets began spreading horizontally by rhizomes. The aboveground biomass was estimated and genets were discriminated in 9-m2 plots placed under both canopy gaps and closed canopies. Overall, the results suggest that the survival and spread of more productive genets and the spatial expansion of genets into closed canopies underlie the proliferation of S. kurilensis. Compared to canopy gaps, the recovery rate of biomass was much slower under closed canopies for the first 10 years after the die-off, but became accelerated during the next seven years. Genet survival was greater for more productive genets (with greater initial number of culms), and the spaces occupied by genets that died were often colonized afterward by clonal growth of surviving genets. The number of genets decreased under canopy gaps due to greater mortality, but increased under closed canopies where greater number of genets colonized clonally from outside the plots than genets died. The colonizing genets were more productive (having larger culms) than those originally germinated within the plots, and the contribution of colonizing genets to the biomass was greater under closed canopies. Our study emphasizes the importance of investigating genet dynamics over relevant spatio-temporal scales to reveal processes underlying the success of clonal plants in heterogeneous habitats.

Data from: Evidence for selection-by-environment but not genotype-by-environment interactions for fitness-related traits in a wild mammal population

How do environmental conditions influence selection and genetic variation in wild populations? There is widespread evidence for selection-by-environment interactions (S*E), but we reviewed studies of natural populations estimating the extent of genotype-by-environment interactions (G*E) in response to natural variation in environmental conditions, and found that evidence for G*E appears to be rare within single populations in the wild. Studies estimating the simultaneous impact of environmental variation on both selection and genetic variation are especially scarce. Here, we used 24 years of data collected from a wild Soay sheep population to quantify how an important environmental variable, population density, impacts upon (1) selection through annual contribution to fitness and (2) expression of genetic variation, in six morphological and life-history traits: body weight; hind leg length; parasite burden; horn length; horn growth; and testicular circumference. Our results supported the existence of S*E: selection was stronger in years of higher population density in all traits apart from horn growth, with directional selection being stronger under more adverse conditions. Quantitative genetic models revealed significant additive genetic variance for body weight, leg length, parasite burden, horn length and testes size, but not for horn growth or our measure of annual fitness. However, random regression models found variation between individuals in their responses to the environment in only three traits, and did not support the presence of G*E for any trait. Our analyses of St Kilda Soay sheep data thus concurs with our cross-study review that, while natural environmental variation within a population can profoundly alter the strength of selection on phenotypic traits, there is less evidence for its effect on the expression of genetic variance in the wild.