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699 results for “Biofilm”
Three-dimensional biofilm growth supports a mutualism involving matrix and nutrient sharing - related scripts and data
<p>This dataset contains the supporting scripts and data used to analyze co-culture fluorescent colony images in high-throughput. It contains some example images that can be used in the scripts.</p> <p>It also contains the script used to plot and determine the maximum growth rates of growth curves of some of the mutants and the script used to stitch overlapping microscope images.</p>
Data from: Quorum sensing and cheating in bacterial biofilms
The idea from human societies that self-interest can lead to a breakdown of cooperation at the group level is sometimes termed the public goods dilemma. We tested this idea in the opportunistic bacterial pathogen, Pseudomonas aeruginosa, by examining the influence of putative cheats that do not cooperate via cell-to-cell signalling (quorum-sensing, QS). We found that: (i) QS cheating occurs in biofilm populations owing to exploitation of QS-regulated public goods; (ii) the thickness and density of biofilms was reduced by the presence of non-cooperative cheats; (iii) population growth was reduced by the presence of cheats, and this reduction was greater in biofilms than in planktonic populations; (iv) the susceptibility of biofilms to antibiotics was increased by the presence of cheats; and (v) coercing cooperator cells to increase their level of cooperation decreases the extent to which the presence of cheats reduces population productivity. Our results provide clear support that conflict over public goods reduces population fitness in bacterial biofilms, and that this effect is greater than in planktonic populations. Finally, we discuss the clinical implications that arise from altering the susceptibility to antibiotics.
Data from: Spatial and successional dynamics of microbial biofilm communities in a grassland stream ecosystem
Biofilms represent a metabolically active and structurally complex component of freshwater ecosystems. Ephemeral prairie streams are hydrologically harsh and prone to frequent perturbation. Elucidating both functional and structural community changes over time within prairie streams provides a general understanding of microbial responses to environmental disturbance. We examined microbial succession of biofilm communities at three sites in a third-order stream at Konza Prairie over a 2- to 64-day period. Microbial abundance (bacterial abundance, chlorophyll a concentrations) increased and never plateaued during the experiment. Net primary productivity (net balance of oxygen consumption and production) of the developing biofilms did not differ statistically from zero until 64 days suggesting a balance of the use of autochthonous and allochthonous energy sources until late succession. Bacterial communities (MiSeq analyses of the V4 region of 16S rRNA) established quickly. Bacterial richness, diversity and evenness were high after 2 days and increased over time. Several dominant bacterial phyla (Beta-, Alphaproteobacteria, Bacteroidetes, Gemmatimonadetes, Acidobacteria, Chloroflexi) and genera (Luteolibacter, Flavobacterium, Gemmatimonas, Hydrogenophaga) differed in relative abundance over space and time. Bacterial community composition differed across both space and successional time. Pairwise comparisons of phylogenetic turnover in bacterial community composition indicated that early-stage succession (≤16 days) was driven by stochastic processes, whereas later stages were driven by deterministic selection regardless of site. Our data suggest that microbial biofilms predictably develop both functionally and structurally indicating distinct successional trajectories of bacterial communities in this ecosystem.
Data from: Biofilm morphotypes and population structure among Staphylococcus epidermidis from commensal and clinical samples
Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host's immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in biofilm formation in 98 S. epidermidis isolates from various sources, and investigated population structure based on ribosomal multilocus typing (rMLST) and the presence/absence of genes involved in adhesion and biofilm formation. All isolates were able to adhere and form biofilms in in vitro growth assays and confocal microscopy allowed classification into 5 biofilm morphotypes based on their thickness, biovolume and roughness. Phylogenetic reconstruction grouped isolates into three separate clades, with the isolates in the main disease associated clade displaying diversity in morphotype. Of the biofilm morphology characteristics, only biofilm thickness had a significant association with clade distribution. The distribution of some known adhesion-associated genes (aap and sesE) among isolates showed a significant association with the species clonal frame, with the exception of. These data challenge the assumption that biofilm-associated genes, such as those on the ica operon, are genetic markers for less invasive S. epidermidis isolates, and suggest that phenotypic characteristics, such as adhesion and biofilm formation, are not fixed by clonal descent but are influenced by the presence of various genes that are mobile among lineages.
Supplementary images and table for the manuscript "A-type natriuretic peptide alters the impact of azithromycin on plank-tonic culture and on (monospecies and binary) biofilms of skin bacteria Kytococcus schroeteri and Staphylococcus aureus"
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Peri-implant biofilm HiPR-FISH imaging 2024-04-27 and 2024-05-03
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Peri-implant biofilm HiPR-FISH images 2024-04-24 (2)
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Peri-implant biofilm HiPR-FISH images 2024-04-16 and 2024-04-19(1)
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Peri-implant biofilm HiPR-FISH images 2023-10-18
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Peri-implant biofilm HiPR-FISH images 2022-12-16 and 2023-02-08
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Peri-implant biofilm HiPR-FISH images 2023-10-16
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Peri-implant biofilm HiPR-FISH images 2024-02-18
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Figure 11 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 11. Fibrous brown biofilms that grow in narrow low areas of the landscape of the middle and upper pseudolittoral in presence of water flow. General view of biofilms in a thin layer of water flowing to the sea (a) and fragments of diatom microfouling on the surface of Enteromorpha sp. (b, c). Designations: csh - colonial settlements of Halamphora borealis, ad – araphid diatoms, e.g. Tabularia fasciculata. Scale bar: a - 5 cm, b – 100 µm, c- 10 µm. Photos by Philipp Sapozhnikov, Olga Kalinina.
Figure 8 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 8. Biofilm on clay soil in the "lagoon" (location 1, April 2023). General view of the community in the pseudolittoral landscape (a) and its fragment when assembled x400 (b). Designations: h — cells of different Halamphora species. Scale bar: a - 5 cm, b - 20 µm. Photos by Philipp Sapozhnikov.
Figure 5 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 5. Fragments of biofilms lining the bottom of cauldrons and washout furrows on the tops of sheer cliffs in October 2022. Designations: lm - Lyngbya cf. major, br - Berkeleya rutilans. Scale bar: 20 µm. Photos by Philipp Sapozhnikov.
Figure 4 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 4. Fragments of a black biofilm formed on rocks in the surf zone: a — October 2022, b — April 2023. Scale bar: 20 μm. Designations: gc - Gloeocapsa crepidinum, cg - Calothrix geitonos, cm - Calothrix micromeres, br - Berkeleya rutilans. Photos by Philipp Sapozhnikov.
Figure 7 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 7. Algal-bacterial mat (a) formed in longitudinal puddles (marl folds) on the upper pseudolittoral by mid-April 2023 (location 5). A fragment of the mat at x400 magnification (b), the structural basis is formed by mucous colonies of the smallest cyanobacteria. Designations: np – Navicymbula pusilla, chl – Chroococcus limneticus, lpo - Leptolyngbya ochridana, gl – Gomphosphaeria aponina. Scale bar: a — 5 cm, b — 20 µm. Photos by Philipp Sapozhnikov.
Figure 6 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 6. Fragments of biofilms that covered the bottom of puddles in marl folds on the upper pseudolittoral: a — filamentous green algae; b — microfouling (microepiphyton) on the surface of the thallus of Rhizoclunium sp. Designations: c – Cladophora sp., rh – Rhizoclunium sp., tw - Tabularia waernii, chq - Chroococcus quaternarius. Scale bar: a – 50 µm, b – 20 µm. Photos by Philipp Sapozhnikov.
Figure 2 in Epilithic biofilms of the Eastern Caspian (Aktau region, Kazakhstan) under conditions of falling sea level
Figure 2. Examples of biotops at the newly dry coast of the Caspian Sea in the City of Aktau: a, b - sheer cliffs irrigated by surf; c, d - folded marl forming flat areas of the pseudolittoral. Photos by Philipp Sapozhnikov.
Biofilms manuscript
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International Brain Laboratory public data
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OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.