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1,853 results for “cell culture”
Global gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
Efficient generation of cardiomyocytes from human pluripotent stem cells is critical for their regenerative applications. Microgravity and 3D culture can profoundly modulate cell proliferation and survival. Here we engineered microscale progenitor cardiac spheres from human pluripotent stem cells and exposed the spheres to simulated microgravity using a random positioning machine for 3 days during their differentiation to cardiomyocytes. Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq1000 system. Reads were mapped on the Human Genome Reference and normalized expression table was generated. Results: Among differentially expressed genes 53 of them were up-regulated and 75 were down-regulated. Conclusions: Data demonstrate increased expression of genes associated with growth development and pro-survival in cardiac progenitors cultured under simulated microgravity compared with those cultured under standard gravity. RNA-sequencing analysis was performed to compare global gene expression profiles of cells at differentiation day 8 under simulated microgravity vs. standard gravity.
Comparison of gene expression profiles of normal human bronchial epithelial cells in 2D and 3D cultural conditions
The experiment is part of a project to study DNA repair process after ionizing radiation in organotypic 3-dimentional human bronchial epithlial cell culture. Human bronchial epithelial cells were grown in tissue culture flask (2D) or in matrics gel (3D). Three independent cultures were done for each condition.
A whole-genome microarray study of Arabidopsis thaliana cell cultures exposed to microgravity for 5 days on board of Shenzhou 8
Arabidopsis thaliana wild type cell cultures were exposed to a 5-day space flight onboard of Shenzhou 8 to identify microgravity and space effect related gene expression.
mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity
DLD-1 and MOLT-4 cell lines were cultured in a rotating cell culture system to simulate microgravity and mRNA expression profile in comparison to Static controls. Cells were grown in 10mL rotating vessels in a Rotary Cell Culture System (RCCS) and in 60mm Petri dishes (test control respectively). Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1.
E2F1 mediates competition, proliferation and drug sensitivity of co-cultured resistant and sensitive ovarian cancer cells.
GEO Series GSE244626. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Synergy of Nras mutation and Nup98-Hoxa10HD gene fusion efficiently preserves the stemness of single hematopoietic stem cells after feeder-free culture
GEO Series GSE118809. Mus musculus. 11 samples. Type: Expression profiling by high throughput sequencing.
Comparison of gene expression in human sarcoma cell line SaOS-2 cells between differentiation and maintenance culture
GEO Series GSE71677. Homo sapiens. 8 samples. Type: Expression profiling by array.
HK-2 cell cultured with normal glucose, high glucose, dapagliflozin, metformin and vildagliptin
GEO Series GSE179226. Homo sapiens. 15 samples. Type: Non-coding RNA profiling by array.
Comparison of DNA methylomes of human monocyte-derived dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) generated in co-culture with ovarian carcinoma cell line (OVCAR8) during 6 day
GEO Series GSE103211. Homo sapiens. 6 samples. Type: Methylation profiling by genome tiling array.
Single-cell RNA-sequenceing analysis of mouse intestinal organoids cultured in Matriel or type I collagen
GEO Series GSE300601. Mus musculus. 2 samples. Type: Expression profiling by high throughput sequencing.
Gene expression of LNCaP cells grown in 2D cultures and polyethlene glycol-based hydrogels with the presence of synthetic androgen, R1881
GEO Series GSE33610. Homo sapiens. 12 samples. Type: Expression profiling by array.
Impact of BMP and WNT signaling modulation on mesendoderm progenitor cells specification from hPSCs in 3D culture conditions
GEO Series GSE184302. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
miR-486-5p inhibits eNOS and angiogenesis in cultured endothelial cells by targeting MAML3
GEO Series GSE281065. Homo sapiens. 6 samples. Type: Other.
Transcriptome profiling of influenza-specific memory CD8 T cells following transfer into the airways or in vitro culture
GEO Series GSE137370. Mus musculus. 30 samples. Type: Expression profiling by high throughput sequencing.
Co-culture of hepatic stellate cells and hepatic progenitor cells
GEO Series GSE264276. Mus musculus. 18 samples. Type: Expression profiling by high throughput sequencing.
Fully defined human PSC-derived microglia and tri-culture system reveals cell type specific potentiation of complement C3 production in a model of Alzheimer’s disease [smarter-seq]
GEO Series GSE139549. Homo sapiens. 23 samples. Type: Expression profiling by high throughput sequencing.
Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures
GEO Series GSE292412. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Transcriptional changes on lymphatic endothelial cells upon co-culture with human melanoma cells
GEO Series GSE247542. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome profiles of mouse embryonic stem cells cultured in different conditions by RNA-seq analysis
GEO Series GSE109418. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Analysis of single-cell RNA-seq data from human PBMCs and from in vitro cultures of human cord blood CD34+ progenitors encompassing different DC types
GEO Series GSE115405. Homo sapiens. 3 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.