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11,105 results for “single cells”
Microscopy data for the paper: Analysis and design of single-cell experiments to harvest fluctuation information while rejecting measurement noise.
<p>Microscopy data for the paper: Analysis and design of single-cell experiments to harvest fluctuation information while rejecting measurement noise.</p> <p> </p> <p>List of files used for each dataset.</p> <p> </p> <p>Dataset 0 : MS2-CY5_Cyto543_560_woStim</p> <p> Images in the dataset :</p> <p> ROI001_XY1657814108_Z00_T0_merged.tif - Image Id Number: 0</p> <p> ROI002_XY1657815441_Z00_T0_merged.tif - Image Id Number: 1</p> <p> ROI003_XY1657814110_Z00_T0_merged.tif - Image Id Number: 2</p> <p> ROI004_XY1657814111_Z00_T0_merged.tif - Image Id Number: 3</p> <p> ROI005_XY1657814112_Z00_T0_merged.tif - Image Id Number: 4</p> <p> ROI006_XY1657814113_Z00_T0_merged.tif - Image Id Number: 5</p> <p> ROI007_XY1657814114_Z00_T0_merged.tif - Image Id Number: 6</p> <p> ROI008_XY1657814115_Z00_T0_merged.tif - Image Id Number: 7</p> <p> ROI009_XY1657814116_Z00_T0_merged.tif - Image Id Number: 8</p> <p> ROI010_XY1657814117_Z00_T0_merged.tif - Image Id Number: 9</p> <p> ROI011_XY1657814118_Z00_T0_merged.tif - Image Id Number: 10</p> <p> ROI012_XY1657814119_Z00_T0_merged.tif - Image Id Number: 11</p> <p> </p> <p>Datset 1 : MS2-CY5_Cyto543_560_18minTPL_5uM</p> <p> Images in the dataset :</p> <p> ROI001 - Position 1_XY1657818948_Z00_T0_merged.tif - Image Id Number: 0</p> <p> ROI001 - Position 2_XY1657818949_Z00_T0_merged.tif - Image Id Number: 1</p> <p> ROI001 - Position 4_XY1657818951_Z00_T0_merged.tif - Image Id Number: 2</p> <p> ROI001 - Position 5_XY1657818952_Z00_T0_merged.tif - Image Id Number: 3</p> <p> ROI001 - Position 6_XY1657818953_Z00_T0_merged.tif - Image Id Number: 4</p> <p> ROI001 - Position 7_XY1657818954_Z00_T0_merged.tif - Image Id Number: 5</p> <p> ROI001 - Position 8_XY1657818955_Z00_T0_merged.tif - Image Id Number: 6</p> <p> ROI001 - Position 9_XY1657818956_Z00_T0_merged.tif - Image Id Number: 7</p> <p> ROI001 - Position 10_XY1657818957_Z00_T0_merged.tif - Image Id Number: 8</p> <p> ROI001 - Position 11_XY1657818958_Z00_T0_merged.tif - Image Id Number: 9</p> <p> ROI001 - Position 12_XY1657818959_Z00_T0_merged.tif - Image Id Number: 10</p> <p> </p> <p>Dataset 2: MS2-CY5_Cyto543_560_5hTPL_5uM</p> <p> Images in the datset :</p> <p> ROI001_XY1657822809_Z00_T0_merged.tif - Image Id Number: 0</p> <p> ROI002_XY1657822933_Z00_T0_merged.tif - Image Id Number: 1</p> <p> ROI003_XY1657822934_Z00_T0_merged.tif - Image Id Number: 2</p> <p> ROI005_XY1657822936_Z00_T0_merged.tif - Image Id Number: 3</p> <p> ROI006_XY1657822937_Z00_T0_merged.tif - Image Id Number: 4</p> <p> ROI007_XY1657822938_Z00_T0_merged.tif - Image Id Number: 5</p> <p> ROI008_XY1657822939_Z00_T0_merged.tif - Image Id Number: 6</p> <p> ROI010_XY1657822941_Z00_T0_merged.tif - Image Id Number: 7</p> <p> ROI013_XY1657822944_Z00_T0_merged.tif - Image Id Number: 8</p> <p> ROI014_XY1657822945_Z00_T0_merged.tif - Image Id Number: 9</p> <p> ROI015_XY1657822946_Z00_T0_merged.tif - Image Id Number: 10</p> <p> ROI016_XY1657822947_Z00_T0_merged.tif - Image Id Number: 11</p> <p> ROI017_XY1657822948_Z00_T0_merged.tif - Image Id Number: 12</p> <p> ROI018_XY1657822949_Z00_T0_merged.tif - Image Id Number: 13</p> <p> </p> <p> </p>
Integrated single-cell profiling dissects cell-state-specific enhancer landscapes of human tumor-infiltrating CD8+ T cells_Supplemental_Data
<p>Processed Datasets for:</p> <p>EGA Study ID: EGAS00001006141</p> <p>EGA Dataset ID: EGAD00001008662</p> <p> </p> <p>Find processed files and arrow files</p> <p> </p> <p>Abstract:</p> <p>Despite extensive studies on the chromatin landscape of exhausted T cells, the transcriptional wiring underlying the heterogeneous functional and dysfunctional states of human tumor-infiltrating lymphocytes (TILs) is incompletely understood. Here, we identify gene-regulatory landscapes in a wide breadth of functional and dysfunctional CD8<sup>+</sup> TIL states covering four cancer entities using single-cell chromatin profiling. We map enhancer-promoter interactions in human TILs by integrating single-cell chromatin accessibility with single-cell RNA-seq data from tumor-entity-matching samples and prioritize cell-state-specific genes by super-enhancer analysis. Besides revealing entity-specific chromatin remodeling in exhausted TILs, our analyses identify a common chromatin trajectory to TIL dysfunction and determine key enhancers, transcriptional regulators, and deregulated genes involved in this process. Finally, we validate enhancer regulation at immunotherapeutically relevant loci by targeting non-coding regulatory elements with potent CRISPR activators and repressors. In summary, our study provides a framework for understanding and manipulating cell-state-specific gene-regulatory cues from human tumor-infiltrating lymphocytes.</p>
Database of Ca2+ transients from single cell E. coli measurements
<p><strong>Database configuration</strong><br> To efficiently compare conditions from over 7 Tb of image data, we constructed an HDF5 database with the extracted fluorescent traces for each experiment. The database was configured such that the fluorophore specifies the first group (<em>e.g.,</em> '/gcamp'). The second group is the unique experimental identifier generated at the time of the experiment (<em>e.g.,</em> '/20210322_5 Hz_gcamp_90pts'). The third group specifies the variable name (See Table below for description). The database is also available from https://bitbucket.org/meyerct6/soc_bacteria_repo/src/master/ in (Database/soc_bacteria_database.h5) and can be used to recreate most figures in the manuscript.</p> <table> <caption>Description of HDF5 database variables for each experiment</caption> <tbody> <tr> <td>/meanintMatG</td> <td>Matrix of mean intensity within each segmented cell (rows) at all time points (columns).</td> </tr> <tr> <td>/corrIntMatG</td> <td>Matrix of mean intensity corrected for photobleaching.</td> </tr> <tr> <td>/fitRsqr</td> <td>Vector of R-squared fit values for photobleach equation.</td> </tr> <tr> <td> /timeMat</td> <td>Matrix of acquisition times for each cell (rows) at every timepoint (columns).</td> </tr> <tr> <td>/heat_kill</td> <td>Boolean vector denoting whether the cell was heat killed</td> </tr> </tbody> </table> <p> </p>
Dataset for the paper "Low-Cost Three-Quadrant Single Solar Cell I-V Tracer"
<p>Dataset related to the publication "Low-Cost Three-Quadrant Single Solar Cell I-V Tracer" in Applied Sciences 2022, <em>12</em>(13), 6623; <a href="https://doi.org/10.3390/app12136623">https://doi.org/10.3390/app12136623</a></p>
Processed data and scripts supporting the manuscript "Single-cell transcriptomics reveals immune suppression and cell states predictive of patient outcomes in rhabdomyosarcoma"
<p>This submission contains the compiled count table, processed R objects and various scripts and output files accompanying our manuscript "Single-cell transcriptomics reveals immune suppression and cell states predictive of patient outcomes in rhabdomyosarcoma" (Nature Communications, 2023, https://doi.org/10.1038/s41467-023-38886-8)</p>
Source data for paper "Mapping disease regulatory circuits at cell-type resolution from single-cell multiomics data"
<p>Sample-paired scRNA-seq and scATAC-seq data collected from human peripheral blood mononuclear cells with <em>Staphylococcus aureus</em><em> </em>infection. ScATAC-seq data collected from human peripheral blood mononuclear cells with <em>COVID-19</em> infection. </p>
Gene expression dataset of the Spatially Resolved Single-cell Translatomics at Molecular Resolution
<p>Here are the gene expression datasets of RIBOmap included in "<strong>Spatially Resolved Single-cell Translatomics at Molecular Resolution</strong>" from Zeng et al. Please refer to the README file for more detailed information. </p> <p> </p> <p><strong>Abstract</strong></p> <p>The precise control of mRNA translation is a crucial step in post-transcriptional gene regulation of cellular physiology. However, it remains a major challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of RIBOmap, a three-dimensional (3D) in situ profiling method to detect mRNA translation of thousands of genes simultaneously in intact cells and tissues. By applying RIBOmap to 981 genes in HeLa cells, we revealed a remarkable dependency of translation on cell-cycle stages and subcellular localization. Furthermore, we profiled single-cell translatomes of 5,413 genes in adult mouse brain tissues yielding a spatial cell atlas of 119,173 cells. The pairwise spatial mapping of single-cell translatome and transcriptome in two adjacent mouse brain slices revealed cell-type and brain-region-dependent translational regulation and suggested a translation remodeling during oligodendrocyte lineage maturation. The spatial translatome profiling detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks. Together, RIBOmap presents the first spatially resolved single-cell translatomics technology, accelerating our understanding of protein synthesis in the context of subcellular architecture, cell types, and tissue anatomy.</p>
Supporting data for "Dissecting the cellular architecture of neuroblastoma bone marrow metastasis using single-cell transcriptomics and epigenomics unravels the role of monocytes at the metastatic niche"
<p>This data repository contains several datasets supplementing the paper “Dissecting the cellular architecture of neuroblastoma bone marrow metastasis using single-cell transcriptomics and epigenomics unravels the role of monocytes at the metastatic niche” by Fetahu, Esser-Skala, Dnyansagar et al. (2023).</p> <ul> <li>HOMER_Results.zip: detailed results of the HOMER analysis</li> <li>nblast_scopen_gene_activity_normalized_motifs_added.rds: Seurat object with scATAC-seq data</li> <li>snp_array.tgz: SNP array data</li> <li>R_data_generated.tgz: Files generated by the scRNA-seq analysis scripts in the GitHub repository associated with the publication.</li> </ul>
Comparative Analysis of Droplet- vs. Microwell-based Whole Transcriptome Single-Cell Sequencing Technologies in Complex Human Tissues
<p>In the past decade, high-dimensional single-cell omics tools have enabled scientists to study the tumor microenvironment (TME) in unprecedented detail. However, recent investigations suggest that each technique has its unique strengths but also technology-inherent limitations. Here we directly compared two commercially available high-throughput single-cell RNA sequencing (scRNA-seq) technologies - droplet-based 10X Chromium <em>vs.</em> microwell-based BD Rhapsody - using paired samples from patients with localized prostate cancer (PCa) undergoing a radical prostatectomy.</p> <p>Although high technical consistency was observed in unraveling the whole transcriptome, the relative abundance of detectable cell populations differed. This could in part be ascribed to differences in the performance to recover cells with low-mRNA content. Hence, immune cells such as neutrophils are underrepresented in data generated with the widely used droplet-based scRNA-seq protocol, highlighting the importance of considering platform limitations in low mRNA content cell recovery. In contrast, droplet-based scRNA-seq demonstrated superiority in terms of recovering cells of epithelial origin. Moreover, we discovered platform-dependent variabilities in mRNA quantification and cell-type marker annotation, affecting the composition of identified tissue profiles and the exploratory value of the generated datasets. Overall, our study emphasizes the importance of carefully selecting the appropriate scRNA-seq platform to improve cell type representation and obtain a more comprehensive and accurate understanding of the TME.</p>
Datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells
<p>Different datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells. The repository contains .zip archives, mostly containing a readme file with additional information.</p> <pre>Cell imaging experiments.zip contains the raw image files acquired on arrays of version 1 or version 2 using a Nikon TI inverted microscope and used in figures 4-6. </pre> <p>Gla_0127_14.zip contains SEM images of the fabrication of arrays of version 1</p> <p>Gla_29_Pd_1.zip contains SEM images of the fabrication of arrays of version 2</p> <p>SM experiments.zip contains the raw single-molecule fluorescence data acquired on an array of version 1 using a PicoQuant Microtime microscope together with the analysis files.</p> <p>FDTD simulations.zip contains the simulation files for the use in the software Lumerical</p>
Imaging data from "Live-cell 3D single-molecule tracking reveals modulation of enhancer dynamics by NuRD"
<p>3D 20ms, 3D 500ms and 2D dCas9 raw videos, localisation, tracking and trajectory analysis data</p> <p>From 'Live-cell 3D single-molecule tracking reveals modulation of enhancer dynamics by NuRD" (2021). Biorxiv. https://doi.org/10.1101/2020.04.03.003178</p>
NewSOC, supplementary information to WT2.3 "Development of doped lanthanum chromite based fuel electrodes", WT2.5 "Electrochemical characterization of single cells"
<p>These are experimental data related to CERTH participation in NewSOC project (874577) regarding WT2.3 “Development of doped lanthanum chromite based fuel electrodes”, WT2.5 “Electrochemical characterization of single cells”. The data set includes:</p> <ul> <li>physicochemical characterization (ICP, BET, XRD and SEM) of the powders and electrodes or cells produced (SEM)</li> <li>electrochemical characterization (iV characteristics, impedance, stability tests)</li> </ul> <p> </p> <p><strong>LSCrF0.1.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.9</sub>Fe<sub>0.1</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM, (B) SEM images of electrodes and cells and (C) data set including iV, EIS and stability testing for button cells using different electrolyte substrates (formulation and thickness) and oxygen electrode materials operating under steam electrolysis, co-electrolysis of steam and carbon dioxide and reversible operation in steam or carbon dioxide cycle.</p> <p><strong>LSCrF0.5.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.5</sub>Fe<sub>0.5</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM, (B) SEM images of electrodes and cells and (C) data set including iV and EIS for button cells operating under steam electrolysis and co-electrolysis of steam and carbon dioxide. </p> <p><strong>LSCrNi0.1.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.9</sub>Ni<sub>0.1</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM, (B) SEM images of electrodes and cells and (C) data set including iV and EIS for button cells operating under steam electrolysis and co-electrolysis of steam and carbon dioxide. </p> <p><strong>LSCrNi0.5.rar:</strong> The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.5</sub>Ni<sub>0.5</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM, and (B) data set including iV and EIS for button cells operating under steam electrolysis and co-electrolysis of steam and carbon dioxide.</p> <p><strong>LSCrMn0.1.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.9</sub>Mn<sub>0.1</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM, (B) SEM images of electrodes and cells and (C) data set including iV and EIS for button cells operating under steam electrolysis and co-electrolysis of steam and carbon dioxide. </p> <p><strong>LSCrF0.05Ti0.05.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.9</sub>Fe<sub>0.05</sub>Ti<sub>0.05</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM and (B) data set including iV and EIS for button cells operating under steam electrolysis, co-electrolysis of steam and carbon dioxide.</p> <p><strong>LSCrF0.05V0.05.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.9</sub>Fe<sub>0.05</sub>V<sub>0.05</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM and (B) data set including iV and EIS for button cells operating under steam electrolysis, co-electrolysis of steam and carbon dioxide.</p> <p><strong>LSCrF0.25Ti0.25.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.5</sub>Fe<sub>0.25</sub>Ti<sub>0.25</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM and (B) data set including iV and EIS for button cells operating under steam electrolysis, co-electrolysis of steam and carbon dioxide. </p> <p><strong>LSCrF0.25V0.25.rar</strong>: The zip folder contains data related to La<sub>0.75</sub>Sr<sub>0.25</sub>Cr<sub>0.5</sub>Fe<sub>0.25</sub>V<sub>0.25</sub>O<sub>3-δ</sub> material. (A) powder physicochemical characterization including ICP, BET, XRD and SEM, (B) SEM images of electrodes and cells and (C) data set including iV and EIS for button cells operating under steam electrolysis and co-electrolysis of steam and carbon dioxide.</p> <p><strong>Ni-GDC.rar</strong>: The zip folder contains data related to button cell stability testing using a commercial Ni-GDC fuel electrode material operating under steam electrolysis. </p> <p><strong>LARGE CELLS.rar</strong>: Data set for large cells (5x5 cm2) including iV, EIS and short-term stability testing employing Keracell III cell, Ni-GDC and LSCrF0.1 fuel electrodes operating under steam electrolysis and co-electrolysis of steam and carbon dioxide.</p> <p> </p> <p> </p> <p> </p>
Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing
<p>Supplementary data that are needed to rerun the reproducible notebooks from the first steps using Alevin output and configuration files.</p> <p>Intermediate R data object that can be used to rerun the reproducible notebooks after the filtering steps.</p> <p>Validation data for inferring the sample index hopping rate. The <em>hiseq4000_joined_datatable_plexed_nonplexed.zip file contains read counts for four samples (two non-multiplexed and two multiplexed) joined by a cell-barcode, UMI, and gene-ID (CUG) key combination. The hiseq4000_inner_joined_with_labels.zip file contains only those CUGs that are observed in both the non-multiplexed and multiplexed samples.</em><em> </em></p>
Spatial organization of the mouse retina at single cell resolution by MERFISH
<p><strong>Abstract:</strong><br> <br> The visual signal processing in the retina requires the precise organization of diverse neuronal types working in concert. While single-cell omics studies have identified more than 120 different neuronal subtypes in the mouse retina1, little is known about their spatial organization. Here, we generated the first single-cell spatial atlas of the mouse retina using multiplexed error-robust fluorescence in situ hybridization (MERFISH). We profiled over 390,000 cells and identified all major cell types and nearly all subtypes through the integration with reference single-cell RNA sequencing (scRNA-seq) data. Our spatial atlas allowed simultaneous examination of nearly all cell subtypes in the retina, revealing 8 previously unknown displaced amacrine cell subtypes and establishing the first connection between the molecular classification of many cell subtypes and their spatial arrangement. Furthermore, we identified spatially dependent differential gene expression between subtypes, suggesting the possibility of functional tuning of neuronal types based on location.<br> <br> <strong>Data description:</strong><br> 1. VZA105a_integrated_368genes.h5ad<br> This file contains the raw MERFISH count matrix for four samples with 368 gene features. The "sampleid" column represents the unique sample ID, while the "region" column corresponds to the tissue section ID. The "majorclass" and "subclass" columns indicate annotated retinal cell types. Finally, the "center_x" and "center_y" columns provide the coordinates of the cell centers.</p> <p><br> 2. VA45_integrated.h5ad<br> This file contains the raw MERFISH count matrix for six samples with 500 gene features. The "sampleid" column represents the unique sample ID, while the "region" column corresponds to the tissue section ID. The "majorclass" and "subclass" columns indicate annotated retinal cell types. Finally, the "center_x" and "center_y" columns provide the coordinates of the cell centers.<br> <br> 3. merfish_impute.h5ad<br> This file contains the imputed count matrix for ten samples. The "sampleid" column represents the unique sample ID, while the "region" column corresponds to the tissue section ID. The "majorclass" and "subclass" columns indicate annotated retinal cell types. Finally, the "center_x" and "center_y" columns provide the coordinates of the cell centers.<br> </p>
Supplementary Data: Identification of experimentally-supported poly(A) sites in single-cell RNA-seq data with SCINPAS
<p>Supplementary data for the SCINPAS analysis as part of the manuscript "Identification of experimentally-supported poly(A) sites in single-cell RNA-seq data with SCINPAS".</p> <p>It includes the output of SCINPAS, available here: <a href="https://github.com/zavolanlab/SCINPAS">https://github.com/zavolanlab/SCINPAS</a>, used for the analyses. Additionally, the scripts and data for the comparison with other tools is provided. Please read the README.md for more information.</p>
Analysis Products: Transcription factor stoichiometry, motif affinity and syntax regulate single cell chromatin dynamics during fibroblast reprogramming to pluripotency
<p>This record contains analysis products for the paper "Transcription factor stoichiometry, motif affinity and syntax regulate single cell chromatin dynamics during fibroblast reprogramming to pluripotency" by Nair, Ameen <em>et al</em>. Please refer to the READMEs in the directories, which are summarized below.</p> <p>The record contains the following files:<br> <br> `clusters.tsv`: <strong> </strong>contains the cluster id, name and colour of clusters in the paper</p> <p><strong>scATAC.zip</strong></p> <p>Analysis products for the single-cell ATAC-seq data. Contains:</p> <p>- `cells.tsv`: list of barcodes that pass QC. Columns include:<br> - `barcode`<br> - `sample`: (time point)<br> - `umap1`<br> - `umap2`<br> - `cluster`<br> - `dpt_pseudotime_fibr_root`: pseudotime values treating a fibroblast cell as root<br> - `dpt_pseudotime_xOSK_root`: pseudotime values treating xOSK cell as root<br> - `peaks.bed`: list of peaks of 500bp across all cell states. 4th column contains the peak set label. Note that ~5000 peaks are not assigned to any peak set and are marked as NA.<br> - `features.tsv`: 50 dimensional representation of each cell <br> - `cell_x_peak.mtx.gz`: sparse matrix of fragment counts within peaks. Load using scipy.io.mmread in python or readMM in R. Columns correspond to cells from `cells.tsv` (combine sample + barcode). Rows correspond to peaks in `peaks.bed` </p> <p><strong>scATAC_clusters.zip</strong></p> <p>Analysis products corresponding to cluster pseudo-bulks of the single-cell ATAC-seq data. </p> <p>- `clusters.tsv`: contains the cluster id, name and colour used in the paper<br> - `peaks`: contains `overlap_reproducibilty/overlap.optimal_peak` peaks called using ENCODE bulk ATAC-seq pipeline in the narrowPeak format.<br> - `fragments`: contains per cluster fragment files </p> <p><strong>scATAC_scRNA_integration.zip</strong></p> <p>Analysis products from the integration of scATAC with scRNA. Contains:</p> <p>- `peak_gene_links_fdr1e-4.tsv`: file with peak gene links passing FDR 1e-4. For analyses in the paper, we filter to peaks with absolute correlation >0.45.<br> - `harmony.cca.30.feat.tsv`: 30 dimensional co-embedding for scATAC and scRNA cells obtained by CCA followed by applying Harmony over assay type.<br> - `harmony.cca.metadata.tsv`: UMAP coordinates for scATAC and scRNA cells derived from the Harmony CCA embedding. First column contains barcode.</p> <p><strong>scRNA.zip</strong></p> <p>Analysis products for the single-cell RNA-seq data. Contains:</p> <p>- `seurat.rds`: seurat object that contains expression data (raw counts, normalized, and scaled), reductions (umap, pca), knn graphs, all associated metadata. Note that barcode suffix (1-9 corresponds to samples D0, D2, ..., D14, iPSC)<br> - `genes.txt`: list of all genes<br> - `cells.tsv`: list of barcodes that pass QC across samples. Contains:<br> - `barcode_sample`: barcode with index of sample (1-9 corresponding to D0, D2, ..., D14, iPSC) <br> - `sample`: sample name (D0, D2, .., D14, iPSC)<br> - `umap1`<br> - `umap2`<br> - `nCount_RNA`<br> - `nFeature_RNA`<br> - `cluster`<br> - `percent.mt`: percent of mitochondrial transcripts in cell<br> - `percent.oskm`: percent of OSKM transcripts in cell<br> - `gene_x_cell.mtx.gz`: sparse matrix of gene counts. Load using scipy.io.mmread in python or readMM in R. Columns correspond to cells from `cells.tsv` (barcode suffix contains sample information). Rows correspond to genes in `genes.txt` <br> - `pca.tsv`: first 50 PC of each cell<br> - `oskm_endo_sendai.tsv`: estimated raw counts (cts, may not be integers) and log(1+ tp10k) normalized expression (norm) for endogenous and exogenous (Sendai derived) counts of POU5F1 (OCT4), SOX2, KLF4 and MYC genes. Rows are consistent with `seurat.rds` and `cells.tsv`</p> <p><strong>multiome.zip</strong></p> <p><em>multiome/snATAC:</em></p> <p>These files are derived from the integration of nuclei from multiome (D1M and D2M), with cells from day 2 of scATAC-seq (labeled D2). </p> <p>- `cells.tsv`: This is the list of nuclei barcodes that pass QC from multiome AND also cell barcodes from D2 of scATAC-seq. Includes:<br> - `barcode`<br> - `umap1`: These are the coordinates used for the figures involving multiome in the paper.<br> - `umap2`: ^^^ <br> - `sample`: D1M and D2M correspond to multiome, D2 corresponds to day 2 of scATAC-seq<br> - `cluster`: For multiome barcodes, these are labels transfered from scATAC-seq. For D2 scATAC-seq, it is the original cluster labels. <br> - `peaks.bed`: This is the same file as scATAC/peaks.bed. List of peaks of 500bp. 4th column contains the peak set label. Note that ~5000 peaks are not assigned to any peak set and are marked as NA.<br> - `cell_x_peak.mtx.gz`: sparse matrix of fragment counts within peaks. Load using scipy.io.mmread in python or readMM in R. Columns correspond to cells from `cells.tsv` (combine sample + barcode). Rows correspond to peaks in `peaks.bed`.<br> - `features.no.harmony.50d.tsv`: 50 dimensional representation of each cell prior to running Harmony (to correct for batch effect between D2 scATAC and D1M,D2M snMultiome). Rows correspond to cells from `cells.tsv`.<br> - `features.harmony.10d.tsv`: 10 dimensional representation of each cell after running Harmony. Rows correspond to cells from `cells.tsv`.</p> <p><em>multiome/snRNA:</em></p> <p>- `seurat.rds`: seurat object that contains expression data (raw counts, normalized, and scaled), reductions (umap, pca),associated metadata. Note that barcode suffix (1,2 corresponds to samples D1M, D2M). Please use the UMAP/features from snATAC/ for consistency.<br> - `genes.txt`: list of all genes (this is different from the list in scRNA analysis)<br> - `cells.tsv`: list of barcodes that pass QC across samples. Contains:<br> - `barcode_sample`: barcode with index of sample (1,2 corresponding to D1M, D2M respectively) <br> - `sample`: sample name (D1M, D2M)<br> - `nCount_RNA`<br> - `nFeature_RNA`<br> - `percent.oskm`: percent of OSKM genes in cell<br> - `gene_x_cell.mtx.gz`: sparse matrix of gene counts. Load using scipy.io.mmread in python or readMM in R. Columns correspond to cells from `cells.tsv` (barcode suffix contains sample information). Rows correspond to genes in `genes.txt` </p>
Single-cell RNA sequencing of sclerotome-derived fibroblasts in zebrafish
<p>Despite their importance in tissue maintenance and repair, fibroblast diversity and plasticity remain poorly understood. Using single-cell RNA sequencing, we uncover distinct sclerotome-derived fibroblast populations in zebrafish, including progenitor-like perivascular/interstitial fibroblasts, and specialized fibroblasts such as tenocytes. To determine fibroblast plasticity <em>in vivo</em>, we develop a laser-induced tendon ablation and regeneration model. Lineage tracing reveals that laser-ablated tenocytes are quickly regenerated by preexisting fibroblasts. By combining single-cell clonal analysis and live imaging, we demonstrate that perivascular/interstitial fibroblasts actively migrate to the injury site, where they proliferate and give rise to new tenocytes. By contrast, perivascular fibroblast-derived pericytes or specialized fibroblasts, including tenocytes, exhibit no regenerative plasticity. Interestingly, active Hedgehog (Hh) signaling is required for the proliferation of activated fibroblasts to ensure efficient tenocyte regeneration. Together, our work highlights the functional diversity of fibroblasts and establishes perivascular/interstitial fibroblasts as tenocyte progenitors that promote tendon regeneration in a Hh signaling-dependent manner.</p>
scProAtlas: an atlas of multiplexed single-cell spatial proteomics imaging in human tissues
<p>All analysis results for the spatial proteomics imaging techniques in the scProAtlas database are stored in compressed files named accordingly. Within each compressed file, the folders are organized in a fixed storage structure in the following order: Analysis module > Imaging Technique > Dataset > Tissue > ROI.</p> <p>Each folder contains the corresponding metadata (including original sample information, cell type annotations, and neighborhood annotations) stored in a file named <code>cells.tsv</code>. Additionally, the module used to identify spatial pattern genes includes an <code>anndata</code> format file, named <code>adata_moran.h5ad</code>, which stores the integrated results of scRNA-seq and spatial proteomics.</p> <p>scProAtlas_analysis_code.tar.gz contains example codes for all analysis modules in scProAtlas. Here, we provide the example using <strong>SCP_CODEX1 - Large intestine. </strong>The codes include all the scripts used for the entire workflow, from image segmentation to scRNA-spatial proteomics integration, and spatial analysis.</p> <p>We have also uploaded the raw protein channel matrices with AnnData format in <strong>version 3 and 4.</strong></p>
Vizgen MERFISH files for Single-cell analysis reveals M. tuberculosis ESX-1-mediated accumulation of anti-inflammatory macrophages in infected mouse lungs
Open the record for dataset details and reuse information.
Single-cell analysis reveals M. tuberculosis ESX-1-mediated accumulation of anti-inflammatory macrophages in infected mouse lungs
Open the record for dataset details and reuse information.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.