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5 results for “16S barcodes”
Fig. 2. Minimum spanning network depicting relationships among 16S in Which frog's legs do froggies eat? The use of DNA barcoding for identification of deep frozen frog legs (Dicroglossidae, Amphibia) commercialized in France
Fig. 2. Minimum spanning network depicting relationships among 16S haplotypes of Fejervarya cancrivora (Gravenhorst, 1829). The size of each circle is proportional to the haplotype frequency and the lengths of the connecting lines are proportional to the number of mutations. Colors refer to distinct regions (Indonesia: Java, Sumatra, Bali, Kalimantan, Bangka; Malaysia; Taiwan) and commercialized frogs of unknown origin are in black.
F I G U R E 3 A 16S in Assessing the diversity of Australian tarantulas (Araneae: Theraphosidae) using DNA barcoding and iterative species delimitation
F I G U R E 3 A 16S neighbour-joining tree constructed under an HKY substitution model used for identifying putative species.
Data from: COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)
The 5' region of the mitochondrial DNA (mtDNA) gene cytochrome c oxidase I (COI) is the standard marker for DNA barcoding. However, because COI tends to be highly variable in amphibians, sequencing is often challenging. Consequently, another mtDNA gene, 16S rRNA gene, is often advocated for amphibian barcoding. Herein, we directly compare the usefulness of COI and 16S in discriminating species of hynobiid salamanders using 130 individuals. Species identification and classification of these animals, which are endemic to Asia, is often based on morphology only. Analysis of Kimura 2-parameter genetic distances (K2P) documents the mean intraspecific variation for COI and 16S rRNA genes to be 1.4% and 0.3%, respectively. Whereas COI can always identify species, sometimes 16S cannot. Intra- and interspecific genetic divergences occasionally overlap in both markers thus reducing the value of a barcoding gap to identify genera. Regardless, COI is the better DNA barcoding marker for hynobiids. In addition to the comparison of two potential markers, high levels of intraspecific divergence in COI (>5%) suggest that both Onychodactylus fischeri and Salamandrella keyserlingii might be composites of cryptic species.
Fig. 8. 16S in DNA barcoding of some Pandeidae species (Cnidaria, Hydrozoa, Anthoathecata)
Fig. 8. 16S maximum likelihood phylogenetic tree of Pandeidae species obtained with PhyML (GTR+G+I model) and based on 595 bp positions of the mitochondrial 16S gene. Node-support values are bootstrap values of 100 pseudoreplicates (shown only if> 70%). For more details see text and Table 1. Samples based on the polyp stage are indicated, all others are medusa samples.
Data from: COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)
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Allen Brain Atlas
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International Brain Laboratory public data
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OpenNeuro
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